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Protein

Transcription termination factor 1, mitochondrial

Gene

Mterf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Transcription termination activity appears to be polarized with highest specificity for transcripts initiated on the light strand (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation, Transcription termination

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription termination factor 1, mitochondrial
Alternative name(s):
Mitochondrial transcription termination factor 1
Short name:
mTERF
Gene namesi
Name:Mterf1
Synonyms:Mterf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621318. Mterf1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3737MitochondrionSequence analysisAdd
BLAST
Chaini38 – 374337Transcription termination factor 1, mitochondrialPRO_0000021782Add
BLAST

Post-translational modificationi

Is a phosphoprotein. While the DNA-binding activity is unaffected by the phosphorylation/dephosphorylation state, only the phosphorylated form of the protein is active for termination activity. Functioning seems to be regulated by phosphorylation.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9EPI8.
PRIDEiQ9EPI8.

Interactioni

Subunit structurei

Monomer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei139 – 1391Interaction with DNABy similarity
Sitei179 – 1791Interaction with DNABy similarity
Sitei327 – 3271Interaction with DNABy similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010425.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni146 – 1472Interaction with DNABy similarity
Regioni224 – 2285Interaction with DNABy similarity
Regioni301 – 3088Interaction with DNABy similarity
Regioni332 – 3354Interaction with DNABy similarity
Regioni361 – 3688Interaction with DNABy similarity

Domaini

Contains nine structural repeats of about 35 residues, where each repeat contains three helices. The repeats form a left-handed superhelical assembly with a solenoid structure that wraps itself around DNA (By similarity).By similarity

Sequence similaritiesi

Belongs to the mTERF family.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG1267. Eukaryota.
ENOG410XT49. LUCA.
HOGENOMiHOG000252957.
HOVERGENiHBG052524.
InParanoidiQ9EPI8.
KOiK15031.
PhylomeDBiQ9EPI8.

Family and domain databases

InterProiIPR003690. MTERF.
[Graphical view]
PfamiPF02536. mTERF. 1 hit.
[Graphical view]
SMARTiSM00733. Mterf. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9EPI8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASRNIWRVR RNFLFDLRGW VPQYSAEVFL KSIPFRPFSV ECNSKDGENG
60 70 80 90 100
DLLNNLLTMG VDVDMARRRQ PGVFNKAVTN EQELKMFLLS KGASDKVIGS
110 120 130 140 150
IISRYPRAIT RTPESLSKRW DLWREIMASD LEIVNILERS PESFFRSNNN
160 170 180 190 200
LNLENNIKFL CSVGLTHKCL CRLLTSAPRT FSNSLNLNKQ MVEFLQETGI
210 220 230 240 250
SLGHNNPTDF VRKIISKNPS ILIQSTKRVK TNIEFLQSTF NLDKEDLLLL
260 270 280 290 300
ICGPGARILD LSNDCTKRNY TNIKKRLLSL GCTEEEVQKF VLSYLNMIFL
310 320 330 340 350
SEKKFNDKID CLLEEKISTS QILENPRVLD SSIHTLKTRI RELAHAGYDV
360 370
STSSIALLSW SQRRYEAKLK RLSG
Length:374
Mass (Da):42,937
Last modified:March 1, 2001 - v1
Checksum:i43FD94B40069B3F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ292524 mRNA. Translation: CAC20864.1.
RefSeqiNP_445951.1. NM_053499.1.
UniGeneiRn.64629.

Genome annotation databases

GeneIDi85261.
KEGGirno:85261.
UCSCiRGD:621318. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ292524 mRNA. Translation: CAC20864.1.
RefSeqiNP_445951.1. NM_053499.1.
UniGeneiRn.64629.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010425.

Proteomic databases

PaxDbiQ9EPI8.
PRIDEiQ9EPI8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi85261.
KEGGirno:85261.
UCSCiRGD:621318. rat.

Organism-specific databases

CTDi7978.
RGDi621318. Mterf1.

Phylogenomic databases

eggNOGiKOG1267. Eukaryota.
ENOG410XT49. LUCA.
HOGENOMiHOG000252957.
HOVERGENiHBG052524.
InParanoidiQ9EPI8.
KOiK15031.
PhylomeDBiQ9EPI8.

Miscellaneous databases

PROiQ9EPI8.

Family and domain databases

InterProiIPR003690. MTERF.
[Graphical view]
PfamiPF02536. mTERF. 1 hit.
[Graphical view]
SMARTiSM00733. Mterf. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Phosphorylation of rat mitochondrial transcription termination factor (mTERF) is required for transcription termination but not for binding to DNA."
    Prieto-Martin A., Montoya J., Martinez-Azorin F.
    Nucleic Acids Res. 32:2059-2068(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, PHOSPHORYLATION.
    Tissue: Liver.

Entry informationi

Entry nameiMTEF1_RAT
AccessioniPrimary (citable) accession number: Q9EPI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 1, 2001
Last modified: June 8, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.