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Q9EPH0

- S26A5_RAT

UniProt

Q9EPH0 - S26A5_RAT

Protein

Prestin

Gene

Slc26a5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site By similarity.By similarity

    GO - Molecular functioni

    1. motor activity Source: RGD
    2. secondary active sulfate transmembrane transporter activity Source: InterPro

    GO - Biological processi

    1. metabolic process Source: GOC
    2. regulation of cell shape Source: UniProtKB-KW
    3. regulation of membrane potential Source: Ensembl
    4. sensory perception of sound Source: UniProtKB-KW

    Keywords - Molecular functioni

    Motor protein

    Keywords - Biological processi

    Cell shape, Hearing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prestin
    Alternative name(s):
    Solute carrier family 26 member 5
    Gene namesi
    Name:Slc26a5
    Synonyms:Pres
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi69334. Slc26a5.

    Subcellular locationi

    Cell membrane; Multi-pass membrane protein
    Note: Lateral wall of outer hair cells.

    GO - Cellular componenti

    1. basolateral plasma membrane Source: Ensembl
    2. integral component of membrane Source: UniProtKB-KW
    3. lateral plasma membrane Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi154 – 1541D → N: Shifts the voltage-sensitivity to more negative values. 1 Publication
    Mutagenesisi155 – 1551D → N: Shifts the voltage-sensitivity to more negative values. 1 Publication
    Mutagenesisi169 – 1691E → Q: No effect. 1 Publication
    Mutagenesisi177 – 1771K → Q: No effect. 1 Publication
    Mutagenesisi197 – 1971R → Q: Shifts the voltage-sensitivity to more negative values. 1 Publication
    Mutagenesisi233 – 2331K → Q: Shifts the voltage-sensitivity to more negative values; when associated with Q-235 and Q-236. 1 Publication
    Mutagenesisi235 – 2351K → Q: Shifts the voltage-sensitivity to more negative values; when associated with Q-233 and Q-236. 1 Publication
    Mutagenesisi236 – 2361R → Q: Shifts the voltage-sensitivity to more negative values; when associated with Q-233 and Q-235. 1 Publication
    Mutagenesisi277 – 2771E → Q: Shifts the voltage-sensitivity to slightly more positive values. 1 Publication
    Mutagenesisi281 – 2811R → Q: No effect; when associated with Q-283 and Q-285. 1 Publication
    Mutagenesisi283 – 2831K → Q: No effect; when associated with Q-218 and Q-285. 1 Publication
    Mutagenesisi285 – 2851K → Q: No effect; when associated with Q-281 and Q-283. 1 Publication
    Mutagenesisi332 – 3321D → Q: No effect. 1 Publication
    Mutagenesisi342 – 3421D → Q: Shifts the voltage-sensitivity to more positive values. 1 Publication
    Mutagenesisi409 – 4091K → Q: No effect. 1 Publication
    Mutagenesisi557 – 5571K → Q: No effect; when associated with Q-558 and Q-559. 1 Publication
    Mutagenesisi558 – 5581R → Q: No effect; when associated with Q-557 and Q-559. 1 Publication
    Mutagenesisi559 – 5591K → Q: No effect; when associated with Q-557 and Q-558. 1 Publication
    Mutagenesisi571 – 5711R → Q: Shifts the voltage-sensitivity to slightly more positive values; when associated with Q-572 and Q-577. 1 Publication
    Mutagenesisi572 – 5721R → Q: Shifts the voltage-sensitivity to slightly more positive values; when associated with Q-571 and Q-577. 1 Publication
    Mutagenesisi577 – 5771K → Q: Shifts the voltage-sensitivity to slightly more positive values; when associated with Q-571 and Q-572. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 744744PrestinPRO_0000080170Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9EPH0.
    PRIDEiQ9EPH0.

    Expressioni

    Tissue specificityi

    Specifically expressed in outer hair cells. Not detected in other cells of the organ of Corti.1 Publication

    Developmental stagei

    Low levels are present in newborn rats and up to day 6. Subsequently, levels increase strongly. Adult levels are detected starting from day 9 in the basal turn of the cochlea, from day 10-11 in the middle turn, and from day 12 in the apical turn.

    Gene expression databases

    GenevestigatoriQ9EPH0.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000015733.

    Structurei

    Secondary structure

    1
    744
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi507 – 5137
    Beta strandi520 – 5223
    Turni523 – 5253
    Beta strandi535 – 5406
    Helixi544 – 55411
    Beta strandi640 – 6456
    Helixi654 – 66815
    Turni669 – 6713
    Beta strandi673 – 6786
    Helixi681 – 6899
    Turni690 – 6934
    Helixi696 – 7016
    Beta strandi702 – 7054
    Helixi706 – 7127

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3LLOX-ray1.57A505-563[»]
    A637-718[»]
    ProteinModelPortaliQ9EPH0.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9EPH0.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 7979CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini101 – 1022ExtracellularSequence Analysis
    Topological domaini124 – 1318CytoplasmicSequence Analysis
    Topological domaini153 – 18331ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini205 – 2117CytoplasmicSequence Analysis
    Topological domaini233 – 25321ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini275 – 28612CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini308 – 33427ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini356 – 37419CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini396 – 41116ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini433 – 4419CytoplasmicSequence Analysis
    Topological domaini463 – 47917ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini501 – 744244CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei80 – 10021Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei103 – 12321Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei132 – 15221Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei184 – 20421Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei212 – 23221Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei254 – 27421Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei287 – 30721Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei335 – 35521Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei375 – 39521Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei412 – 43221Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei442 – 46221Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei480 – 50021Helical; Name=12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini525 – 713189STASPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 STAS domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0659.
    GeneTreeiENSGT00750000117367.
    HOGENOMiHOG000006546.
    HOVERGENiHBG000639.
    InParanoidiQ9EPH0.
    KOiK14703.
    OMAiQRFMPPG.
    OrthoDBiEOG76T9QT.
    PhylomeDBiQ9EPH0.
    TreeFamiTF313784.

    Family and domain databases

    Gene3Di3.30.750.24. 2 hits.
    InterProiIPR018045. S04_transporter_CS.
    IPR002645. STAS_dom.
    IPR001902. SulP_transpt.
    IPR011547. Sulph_transpt.
    [Graphical view]
    PfamiPF01740. STAS. 1 hit.
    PF00916. Sulfate_transp. 1 hit.
    [Graphical view]
    SUPFAMiSSF52091. SSF52091. 2 hits.
    TIGRFAMsiTIGR00815. sulP. 1 hit.
    PROSITEiPS01130. SLC26A. 1 hit.
    PS50801. STAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9EPH0-1 [UniParc]FASTAAdd to Basket

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    MDHAEENEIP AETQKYLVER PIFSHPVLQE RLHVKDKVTD SIGDKLKQAF    50
    TCTPKKVRNI IYMFLPITKW LPAYKFKEYV LGDLVSGIST GVLQLPQGLA 100
    FAMLAAVPPV FGLYSSFYPV IMYCFFGTSR HISIGPFAVI SLMIGGVAVR 150
    LVPDDIVIPG GVNATNGTEA RDALRVKVAM SVTLLSGIIQ FCLGVCRFGF 200
    VAIYLTEPLV RGFTTAAAVH VFTSMLKYLF GVKTKRYSGI FSVVYSTVAV 250
    LQNVKNLNVC SLGVGLMVFG LLLGGKEFNE RFKEKLPAPI PLEFFAVVMG 300
    TGISAGFNLH ESYSVDVVGT LPLGLLPPAN PDTSLFHLVY VDAIAIAIVG 350
    FSVTISMAKT LANKHGYQVD GNQELIALGI CNSIGSLFQT FSISCSLSRS 400
    LVQEGTGGKT QLAGCLASLM ILLVILATGF LFESLPQAVL SAIVIVNLKG 450
    MFMQFSDLPF FWRTSKIELT IWLTTFVSSL FLGLDYGLIT AVIIALLTVI 500
    YRTQSPSYTV LGQLPDTDVY IDIDAYEEVK EIPGIKIFQI NAPIYYANSD 550
    LYSSALKRKT GVNPAIIMGA RRKAMRKYAK EVGNANIANA TVVKVDAEVD 600
    GENATKPEEE DDEVKFPPIV IKTTFPEELQ RFLPQGENIH TVILDFTQVN 650
    FMDSVGVKTL AGIVKEYGDV GIYVYLAGCS AQVVNDLTSN RFFENPALKE 700
    LLFHSIHDAV LGSQVREAMA EQETTVLPPQ EDMEPNATPT TPEA 744
    Length:744
    Mass (Da):81,279
    Last modified:March 1, 2001 - v1
    Checksum:iE49E842CF7A3CD58
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti251 – 2511L → V in AAG30297. 1 PublicationCurated
    Sequence conflicti567 – 5671I → M in AAG30297. 1 PublicationCurated
    Sequence conflicti572 – 5721R → S in AAG30297. 1 PublicationCurated
    Sequence conflicti612 – 6121D → G in AAG30297. 1 PublicationCurated
    Sequence conflicti662 – 6632GI → VM in AAG30297. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ303372 mRNA. Translation: CAC21555.1.
    AF315652 mRNA. Translation: AAG30297.1.
    AJ428404 Genomic DNA. Translation: CAD21439.1.
    RefSeqiNP_110467.1. NM_030840.1.
    UniGeneiRn.64631.

    Genome annotation databases

    EnsembliENSRNOT00000015733; ENSRNOP00000015733; ENSRNOG00000011616.
    GeneIDi83819.
    KEGGirno:83819.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Pump up the volume - Issue 22 of May 2002

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ303372 mRNA. Translation: CAC21555.1 .
    AF315652 mRNA. Translation: AAG30297.1 .
    AJ428404 Genomic DNA. Translation: CAD21439.1 .
    RefSeqi NP_110467.1. NM_030840.1.
    UniGenei Rn.64631.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3LLO X-ray 1.57 A 505-563 [» ]
    A 637-718 [» ]
    ProteinModelPortali Q9EPH0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000015733.

    Proteomic databases

    PaxDbi Q9EPH0.
    PRIDEi Q9EPH0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000015733 ; ENSRNOP00000015733 ; ENSRNOG00000011616 .
    GeneIDi 83819.
    KEGGi rno:83819.

    Organism-specific databases

    CTDi 375611.
    RGDi 69334. Slc26a5.

    Phylogenomic databases

    eggNOGi COG0659.
    GeneTreei ENSGT00750000117367.
    HOGENOMi HOG000006546.
    HOVERGENi HBG000639.
    InParanoidi Q9EPH0.
    KOi K14703.
    OMAi QRFMPPG.
    OrthoDBi EOG76T9QT.
    PhylomeDBi Q9EPH0.
    TreeFami TF313784.

    Miscellaneous databases

    EvolutionaryTracei Q9EPH0.
    NextBioi 616430.
    PROi Q9EPH0.

    Gene expression databases

    Genevestigatori Q9EPH0.

    Family and domain databases

    Gene3Di 3.30.750.24. 2 hits.
    InterProi IPR018045. S04_transporter_CS.
    IPR002645. STAS_dom.
    IPR001902. SulP_transpt.
    IPR011547. Sulph_transpt.
    [Graphical view ]
    Pfami PF01740. STAS. 1 hit.
    PF00916. Sulfate_transp. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52091. SSF52091. 2 hits.
    TIGRFAMsi TIGR00815. sulP. 1 hit.
    PROSITEi PS01130. SLC26A. 1 hit.
    PS50801. STAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Reciprocal electromechanical properties of rat prestin: the motor molecule from rat outer hair cells."
      Ludwig J., Oliver D., Frank G., Kloecker N., Gummer A.W., Fakler B.
      Proc. Natl. Acad. Sci. U.S.A. 98:4178-4183(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TOPOLOGY.
      Tissue: Cochlea.
    2. "Dynamic developmental expression of cochlear hair cell genes: prestin and otoferlin."
      Beisel K.W., Nelson N.C., Beisel C.L., Delimont D.C., He D.Z.Z., Fritzsch B.
      Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE OF 249-668.
      Strain: Sprague-Dawley.
    3. "Thyroid horomone is a critical determinant for the regulation of the cochlear motor protein prestin."
      Weber T., Zimmermann U., Winter H., Mack A., Koepschall I., Rohbock K., Zenner H.P., Knipper M.
      Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE OF 1-20.
      Strain: Sprague-Dawley.
    4. "Expression and localization of prestin and the sugar transporter GLUT-5 during development of electromotility in cochlear outer hair cells."
      Belyantseva I.A., Adler H.J., Curi R., Frolenkov G.I., Kachar B.
      J. Neurosci. 20:RC116-RC116(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    5. "Intracellular anions as the voltage sensor of prestin, the outer hair cell motor protein."
      Oliver D., He D.Z.Z., Kloecker N., Ludwig J., Schulte U., Waldegger S., Ruppersberg J.P., Dallos P., Fakler B.
      Science 292:2340-2343(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: MODE OF ACTION, MUTAGENESIS OF ASP-154; ASP-155; GLU-169; LYS-177; ARG-197; LYS-233; LYS-235; ARG-236; GLU-277; ARG-281; LYS-283; LYS-285; ASP-332; ASP-342; LYS-409; LYS-557; ARG-558; LYS-559; ARG-571; ARG-572 AND LYS-577.

    Entry informationi

    Entry nameiS26A5_RAT
    AccessioniPrimary (citable) accession number: Q9EPH0
    Secondary accession number(s): Q9ERC6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3