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Protein

Prestin

Gene

Slc26a5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • anion transmembrane transport Source: RGD
  • bicarbonate transport Source: GO_Central
  • chloride transmembrane transport Source: RGD
  • cochlea development Source: RGD
  • fructose transport Source: RGD
  • ion transmembrane transport Source: RGD
  • negative regulation of ion transmembrane transport Source: RGD
  • positive regulation of cell motility Source: RGD
  • positive regulation of cell size Source: RGD
  • protein tetramerization Source: RGD
  • regulation of cell shape Source: UniProtKB-KW
  • regulation of intracellular pH Source: GO_Central
  • regulation of membrane potential Source: GO_Central
  • response to auditory stimulus Source: RGD
  • response to drug Source: RGD
  • response to ischemia Source: RGD
  • response to potassium ion Source: RGD
  • response to salicylic acid Source: RGD
  • response to salt Source: RGD
  • response to thyroid hormone Source: RGD
  • sensory perception of sound Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell shape, Hearing

Names & Taxonomyi

Protein namesi
Recommended name:
Prestin
Alternative name(s):
Solute carrier family 26 member 5
Gene namesi
Name:Slc26a5
Synonyms:Pres
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi69334. Slc26a5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 79CytoplasmicSequence analysisAdd BLAST79
Transmembranei80 – 100Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini101 – 102ExtracellularSequence analysis2
Transmembranei103 – 123Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini124 – 131CytoplasmicSequence analysis8
Transmembranei132 – 152Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini153 – 183ExtracellularSequence analysisAdd BLAST31
Transmembranei184 – 204Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini205 – 211CytoplasmicSequence analysis7
Transmembranei212 – 232Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini233 – 253ExtracellularSequence analysisAdd BLAST21
Transmembranei254 – 274Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini275 – 286CytoplasmicSequence analysisAdd BLAST12
Transmembranei287 – 307Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini308 – 334ExtracellularSequence analysisAdd BLAST27
Transmembranei335 – 355Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini356 – 374CytoplasmicSequence analysisAdd BLAST19
Transmembranei375 – 395Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini396 – 411ExtracellularSequence analysisAdd BLAST16
Transmembranei412 – 432Helical; Name=10Sequence analysisAdd BLAST21
Topological domaini433 – 441CytoplasmicSequence analysis9
Transmembranei442 – 462Helical; Name=11Sequence analysisAdd BLAST21
Topological domaini463 – 479ExtracellularSequence analysisAdd BLAST17
Transmembranei480 – 500Helical; Name=12Sequence analysisAdd BLAST21
Topological domaini501 – 744CytoplasmicSequence analysisAdd BLAST244

GO - Cellular componenti

  • basolateral plasma membrane Source: RGD
  • cytoplasm Source: RGD
  • integral component of plasma membrane Source: GO_Central
  • lateral plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi154D → N: Shifts the voltage-sensitivity to more negative values. 1 Publication1
Mutagenesisi155D → N: Shifts the voltage-sensitivity to more negative values. 1 Publication1
Mutagenesisi169E → Q: No effect. 1 Publication1
Mutagenesisi177K → Q: No effect. 1 Publication1
Mutagenesisi197R → Q: Shifts the voltage-sensitivity to more negative values. 1 Publication1
Mutagenesisi233K → Q: Shifts the voltage-sensitivity to more negative values; when associated with Q-235 and Q-236. 1 Publication1
Mutagenesisi235K → Q: Shifts the voltage-sensitivity to more negative values; when associated with Q-233 and Q-236. 1 Publication1
Mutagenesisi236R → Q: Shifts the voltage-sensitivity to more negative values; when associated with Q-233 and Q-235. 1 Publication1
Mutagenesisi277E → Q: Shifts the voltage-sensitivity to slightly more positive values. 1 Publication1
Mutagenesisi281R → Q: No effect; when associated with Q-283 and Q-285. 1 Publication1
Mutagenesisi283K → Q: No effect; when associated with Q-218 and Q-285. 1 Publication1
Mutagenesisi285K → Q: No effect; when associated with Q-281 and Q-283. 1 Publication1
Mutagenesisi332D → Q: No effect. 1 Publication1
Mutagenesisi342D → Q: Shifts the voltage-sensitivity to more positive values. 1 Publication1
Mutagenesisi409K → Q: No effect. 1 Publication1
Mutagenesisi557K → Q: No effect; when associated with Q-558 and Q-559. 1 Publication1
Mutagenesisi558R → Q: No effect; when associated with Q-557 and Q-559. 1 Publication1
Mutagenesisi559K → Q: No effect; when associated with Q-557 and Q-558. 1 Publication1
Mutagenesisi571R → Q: Shifts the voltage-sensitivity to slightly more positive values; when associated with Q-572 and Q-577. 1 Publication1
Mutagenesisi572R → Q: Shifts the voltage-sensitivity to slightly more positive values; when associated with Q-571 and Q-577. 1 Publication1
Mutagenesisi577K → Q: Shifts the voltage-sensitivity to slightly more positive values; when associated with Q-571 and Q-572. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000801701 – 744PrestinAdd BLAST744

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi163N-linked (GlcNAc...)Sequence analysis1
Glycosylationi166N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9EPH0.
PRIDEiQ9EPH0.

PTM databases

iPTMnetiQ9EPH0.
PhosphoSitePlusiQ9EPH0.

Expressioni

Tissue specificityi

Specifically expressed in outer hair cells. Not detected in other cells of the organ of Corti.1 Publication

Developmental stagei

Low levels are present in newborn rats and up to day 6. Subsequently, levels increase strongly. Adult levels are detected starting from day 9 in the basal turn of the cochlea, from day 10-11 in the middle turn, and from day 12 in the apical turn.

Gene expression databases

BgeeiENSRNOG00000011616.
GenevisibleiQ9EPH0. RN.

Interactioni

GO - Molecular functioni

  • protein homodimerization activity Source: RGD
  • transcription factor binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015733.

Structurei

Secondary structure

1744
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi507 – 513Combined sources7
Beta strandi520 – 522Combined sources3
Turni523 – 525Combined sources3
Beta strandi535 – 540Combined sources6
Helixi544 – 554Combined sources11
Beta strandi640 – 645Combined sources6
Helixi654 – 668Combined sources15
Turni669 – 671Combined sources3
Beta strandi673 – 678Combined sources6
Helixi681 – 689Combined sources9
Turni690 – 693Combined sources4
Helixi696 – 701Combined sources6
Beta strandi702 – 705Combined sources4
Helixi706 – 712Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LLOX-ray1.57A505-563[»]
A637-718[»]
5EUSX-ray1.83A505-563[»]
A637-718[»]
5EUUX-ray1.87A505-563[»]
A637-718[»]
5EUWX-ray1.81A505-563[»]
A637-718[»]
5EUXX-ray2.04A505-563[»]
A637-718[»]
5EUZX-ray2.40A505-563[»]
A637-718[»]
ProteinModelPortaliQ9EPH0.
SMRiQ9EPH0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9EPH0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini525 – 713STASPROSITE-ProRule annotationAdd BLAST189

Sequence similaritiesi

Contains 1 STAS domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0236. Eukaryota.
COG0659. LUCA.
GeneTreeiENSGT00760000119026.
HOGENOMiHOG000006546.
HOVERGENiHBG000639.
InParanoidiQ9EPH0.
KOiK14703.
OMAiVGNANMA.
OrthoDBiEOG091G07RT.
PhylomeDBiQ9EPH0.
TreeFamiTF313784.

Family and domain databases

Gene3Di3.30.750.24. 2 hits.
InterProiIPR030282. Prestin.
IPR018045. S04_transporter_CS.
IPR011547. SLC26A/SulP_dom.
IPR001902. SLC26A/SulP_fam.
IPR002645. STAS_dom.
[Graphical view]
PANTHERiPTHR11814. PTHR11814. 2 hits.
PTHR11814:SF32. PTHR11814:SF32. 2 hits.
PfamiPF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMiSSF52091. SSF52091. 2 hits.
TIGRFAMsiTIGR00815. sulP. 1 hit.
PROSITEiPS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EPH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDHAEENEIP AETQKYLVER PIFSHPVLQE RLHVKDKVTD SIGDKLKQAF
60 70 80 90 100
TCTPKKVRNI IYMFLPITKW LPAYKFKEYV LGDLVSGIST GVLQLPQGLA
110 120 130 140 150
FAMLAAVPPV FGLYSSFYPV IMYCFFGTSR HISIGPFAVI SLMIGGVAVR
160 170 180 190 200
LVPDDIVIPG GVNATNGTEA RDALRVKVAM SVTLLSGIIQ FCLGVCRFGF
210 220 230 240 250
VAIYLTEPLV RGFTTAAAVH VFTSMLKYLF GVKTKRYSGI FSVVYSTVAV
260 270 280 290 300
LQNVKNLNVC SLGVGLMVFG LLLGGKEFNE RFKEKLPAPI PLEFFAVVMG
310 320 330 340 350
TGISAGFNLH ESYSVDVVGT LPLGLLPPAN PDTSLFHLVY VDAIAIAIVG
360 370 380 390 400
FSVTISMAKT LANKHGYQVD GNQELIALGI CNSIGSLFQT FSISCSLSRS
410 420 430 440 450
LVQEGTGGKT QLAGCLASLM ILLVILATGF LFESLPQAVL SAIVIVNLKG
460 470 480 490 500
MFMQFSDLPF FWRTSKIELT IWLTTFVSSL FLGLDYGLIT AVIIALLTVI
510 520 530 540 550
YRTQSPSYTV LGQLPDTDVY IDIDAYEEVK EIPGIKIFQI NAPIYYANSD
560 570 580 590 600
LYSSALKRKT GVNPAIIMGA RRKAMRKYAK EVGNANIANA TVVKVDAEVD
610 620 630 640 650
GENATKPEEE DDEVKFPPIV IKTTFPEELQ RFLPQGENIH TVILDFTQVN
660 670 680 690 700
FMDSVGVKTL AGIVKEYGDV GIYVYLAGCS AQVVNDLTSN RFFENPALKE
710 720 730 740
LLFHSIHDAV LGSQVREAMA EQETTVLPPQ EDMEPNATPT TPEA
Length:744
Mass (Da):81,279
Last modified:March 1, 2001 - v1
Checksum:iE49E842CF7A3CD58
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti251L → V in AAG30297 (Ref. 2) Curated1
Sequence conflicti567I → M in AAG30297 (Ref. 2) Curated1
Sequence conflicti572R → S in AAG30297 (Ref. 2) Curated1
Sequence conflicti612D → G in AAG30297 (Ref. 2) Curated1
Sequence conflicti662 – 663GI → VM in AAG30297 (Ref. 2) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ303372 mRNA. Translation: CAC21555.1.
AF315652 mRNA. Translation: AAG30297.1.
AJ428404 Genomic DNA. Translation: CAD21439.1.
RefSeqiNP_110467.1. NM_030840.1.
XP_017448406.1. XM_017592917.1.
UniGeneiRn.64631.

Genome annotation databases

EnsembliENSRNOT00000015733; ENSRNOP00000015733; ENSRNOG00000011616.
GeneIDi83819.
KEGGirno:83819.

Cross-referencesi

Web resourcesi

Protein Spotlight

Pump up the volume - Issue 22 of May 2002

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ303372 mRNA. Translation: CAC21555.1.
AF315652 mRNA. Translation: AAG30297.1.
AJ428404 Genomic DNA. Translation: CAD21439.1.
RefSeqiNP_110467.1. NM_030840.1.
XP_017448406.1. XM_017592917.1.
UniGeneiRn.64631.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LLOX-ray1.57A505-563[»]
A637-718[»]
5EUSX-ray1.83A505-563[»]
A637-718[»]
5EUUX-ray1.87A505-563[»]
A637-718[»]
5EUWX-ray1.81A505-563[»]
A637-718[»]
5EUXX-ray2.04A505-563[»]
A637-718[»]
5EUZX-ray2.40A505-563[»]
A637-718[»]
ProteinModelPortaliQ9EPH0.
SMRiQ9EPH0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015733.

PTM databases

iPTMnetiQ9EPH0.
PhosphoSitePlusiQ9EPH0.

Proteomic databases

PaxDbiQ9EPH0.
PRIDEiQ9EPH0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015733; ENSRNOP00000015733; ENSRNOG00000011616.
GeneIDi83819.
KEGGirno:83819.

Organism-specific databases

CTDi375611.
RGDi69334. Slc26a5.

Phylogenomic databases

eggNOGiKOG0236. Eukaryota.
COG0659. LUCA.
GeneTreeiENSGT00760000119026.
HOGENOMiHOG000006546.
HOVERGENiHBG000639.
InParanoidiQ9EPH0.
KOiK14703.
OMAiVGNANMA.
OrthoDBiEOG091G07RT.
PhylomeDBiQ9EPH0.
TreeFamiTF313784.

Miscellaneous databases

EvolutionaryTraceiQ9EPH0.
PROiQ9EPH0.

Gene expression databases

BgeeiENSRNOG00000011616.
GenevisibleiQ9EPH0. RN.

Family and domain databases

Gene3Di3.30.750.24. 2 hits.
InterProiIPR030282. Prestin.
IPR018045. S04_transporter_CS.
IPR011547. SLC26A/SulP_dom.
IPR001902. SLC26A/SulP_fam.
IPR002645. STAS_dom.
[Graphical view]
PANTHERiPTHR11814. PTHR11814. 2 hits.
PTHR11814:SF32. PTHR11814:SF32. 2 hits.
PfamiPF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMiSSF52091. SSF52091. 2 hits.
TIGRFAMsiTIGR00815. sulP. 1 hit.
PROSITEiPS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS26A5_RAT
AccessioniPrimary (citable) accession number: Q9EPH0
Secondary accession number(s): Q9ERC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.