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Protein

Cell surface glycoprotein MUC18

Gene

Mcam

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Cell surface glycoprotein MUC18
Alternative name(s):
Gicerin
Melanoma cell adhesion molecule
Melanoma-associated antigen MUC18
CD_antigen: CD146
Gene namesi
Name:Mcam
Synonyms:Muc18
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi620463. Mcam.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 560ExtracellularSequence analysisAdd BLAST537
Transmembranei561 – 581HelicalSequence analysisAdd BLAST21
Topological domaini582 – 648CytoplasmicSequence analysisAdd BLAST67

GO - Cellular componenti

  • external side of plasma membrane Source: Ensembl
  • extracellular space Source: RGD
  • focal adhesion Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • nucleus Source: RGD
  • perikaryon Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23By similarityAdd BLAST23
ChainiPRO_000004546124 – 648Cell surface glycoprotein MUC18Add BLAST625

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 118PROSITE-ProRule annotation
Glycosylationi58N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi163 ↔ 225PROSITE-ProRule annotation
Disulfide bondi274 ↔ 322PROSITE-ProRule annotation
Disulfide bondi367 ↔ 409PROSITE-ProRule annotation
Disulfide bondi454 ↔ 501PROSITE-ProRule annotation
Glycosylationi510N-linked (GlcNAc...)Sequence analysis1
Modified residuei608PhosphoserineBy similarity1
Modified residuei616PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9EPF2.
PRIDEiQ9EPF2.

PTM databases

PhosphoSitePlusiQ9EPF2.
SwissPalmiQ9EPF2.

Expressioni

Tissue specificityi

Detected in lung, uterus and placenta (at protein level). Detected in heart, lung, kidney, adrenal gland, intestine, testis, skeletal muscle and aorta. Detected at low levels in adult brain, in particular in brain stem and spinal cord, but also in hippocampus, olfactory bulb and striatum (at protein level).1 Publication

Developmental stagei

Detected at high levels in brain throughout embryonic development (at protein level). Levels are lower in neonates and decrease during the first days after birth (at protein level).

Gene expression databases

BgeeiENSRNOG00000007726.
GenevisibleiQ9EPF2. RN.

Interactioni

Protein-protein interaction databases

BioGridi249375. 1 interactor.
STRINGi10116.ENSRNOP00000010464.

Structurei

3D structure databases

ProteinModelPortaliQ9EPF2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 131Ig-like V-type 1Add BLAST108
Domaini141 – 244Ig-like V-type 2Add BLAST104
Domaini246 – 332Ig-like C2-type 1Add BLAST87
Domaini337 – 426Ig-like C2-type 2Add BLAST90
Domaini432 – 512Ig-like C2-type 3Add BLAST81

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEC2. Eukaryota.
ENOG4110RPG. LUCA.
GeneTreeiENSGT00530000063457.
HOGENOMiHOG000015427.
HOVERGENiHBG002808.
InParanoidiQ9EPF2.
KOiK06534.
OMAiPSRKSEF.
OrthoDBiEOG091G072E.
PhylomeDBiQ9EPF2.

Family and domain databases

Gene3Di2.60.40.10. 5 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
PROSITEiPS50835. IG_LIKE. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9EPF2-1) [UniParc]FASTAAdd to basket
Also known as: L-gicerin

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLPRLVCAF LFAACCCCRS ATGVPGEEKQ PTPTPDPVEV EVGNTALLKC
60 70 80 90 100
GPAHPSGNFS QVEWFLIHKE RQIPIFRVHQ GKGQSEPGEY EHRLSLHGPG
110 120 130 140 150
ATLALSQVTP HDDRMFLCKS KQPRPQDHYV QLQVYKAPEE PTIQANVLGI
160 170 180 190 200
HVDIQELKEV ATCVGRNGYP IPQVIWYKNG RPLQEEENRV HIQSSQTVES
210 220 230 240 250
SGLYTLKSVL SARLVKEDKD AQFYCELSYR LPSGNRMKES KEVTVPVLYP
260 270 280 290 300
AEKVWVEVEP VGLLKEGDHV KIRCLTDGNP QPHFTINKKN PSTEEMEEES
310 320 330 340 350
TDENGLLSLE PAQKHHSGVY QCQSLDLETT VMLSSDPLEL LVNYVSDVQV
360 370 380 390 400
DPTAPEVQEG DSLTLTCKAE SNQDLEFEWL RDKTGQLLGK GPILQLNNVK
410 420 430 440 450
REAGGRYLCV ASVPSVPGLN RTRRVSVGIF GSPWMAAKER KVWAQENAML
460 470 480 490 500
NLSCEASGHP QPTISWNING SATEWNPDPQ TVVSTLNVLV TPELLETGAE
510 520 530 540 550
CTASNSLGSN TTVIILKLVT LTTLTPDSSQ TTGLSTPTVS PHSRANSTST
560 570 580 590 600
EKKLPQQESK GVVIVAVIVC TLVLAVLGAT LYYFYKKGKL PCGRSGKQEI
610 620 630 640
TLPPTRKSEF VVEVKSDKLP EEMALLQGSN GDKRAPGDQG EKYIDLRH
Length:648
Mass (Da):71,327
Last modified:January 10, 2006 - v2
Checksum:i85B727C60D4BF4C3
GO
Isoform 2 (identifier: Q9EPF2-2) [UniParc]FASTAAdd to basket
Also known as: S-gicerin

The sequence of this isoform differs from the canonical sequence as follows:
     600-648: ITLPPTRKSEFVVEVKSDKLPEEMALLQGSNGDKRAPGDQGEKYIDLRH → MERNTSI

Show »
Length:606
Mass (Da):66,683
Checksum:i157F859B6C5EBF28
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti214L → V in BAB16048 (PubMed:14755543).Curated1
Sequence conflicti214L → V in BAB16049 (PubMed:14755543).Curated1
Sequence conflicti227L → V in BAB16048 (PubMed:14755543).Curated1
Sequence conflicti227L → V in BAB16049 (PubMed:14755543).Curated1
Sequence conflicti231L → P in BAB16048 (PubMed:14755543).Curated1
Sequence conflicti231L → P in BAB16049 (PubMed:14755543).Curated1
Sequence conflicti510N → Y in BAB16048 (PubMed:14755543).Curated1
Sequence conflicti510N → Y in BAB16049 (PubMed:14755543).Curated1
Sequence conflicti526P → H in BAB16048 (PubMed:14755543).Curated1
Sequence conflicti526P → H in BAB16049 (PubMed:14755543).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016941600 – 648ITLPP…IDLRH → MERNTSI in isoform 2. 2 PublicationsAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035506 mRNA. Translation: BAB16048.1.
AB035507 mRNA. Translation: BAB16049.1.
BC070916 mRNA. Translation: AAH70916.1.
RefSeqiNP_001029181.1. NM_001034009.1. [Q9EPF2-2]
NP_076473.2. NM_023983.3. [Q9EPF2-1]
UniGeneiRn.2694.

Genome annotation databases

EnsembliENSRNOT00000010463; ENSRNOP00000010464; ENSRNOG00000007726. [Q9EPF2-1]
ENSRNOT00000090780; ENSRNOP00000069860; ENSRNOG00000007726. [Q9EPF2-2]
GeneIDi78967.
KEGGirno:78967.
UCSCiRGD:620463. rat. [Q9EPF2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035506 mRNA. Translation: BAB16048.1.
AB035507 mRNA. Translation: BAB16049.1.
BC070916 mRNA. Translation: AAH70916.1.
RefSeqiNP_001029181.1. NM_001034009.1. [Q9EPF2-2]
NP_076473.2. NM_023983.3. [Q9EPF2-1]
UniGeneiRn.2694.

3D structure databases

ProteinModelPortaliQ9EPF2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249375. 1 interactor.
STRINGi10116.ENSRNOP00000010464.

PTM databases

PhosphoSitePlusiQ9EPF2.
SwissPalmiQ9EPF2.

Proteomic databases

PaxDbiQ9EPF2.
PRIDEiQ9EPF2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010463; ENSRNOP00000010464; ENSRNOG00000007726. [Q9EPF2-1]
ENSRNOT00000090780; ENSRNOP00000069860; ENSRNOG00000007726. [Q9EPF2-2]
GeneIDi78967.
KEGGirno:78967.
UCSCiRGD:620463. rat. [Q9EPF2-1]

Organism-specific databases

CTDi4162.
RGDi620463. Mcam.

Phylogenomic databases

eggNOGiENOG410IEC2. Eukaryota.
ENOG4110RPG. LUCA.
GeneTreeiENSGT00530000063457.
HOGENOMiHOG000015427.
HOVERGENiHBG002808.
InParanoidiQ9EPF2.
KOiK06534.
OMAiPSRKSEF.
OrthoDBiEOG091G072E.
PhylomeDBiQ9EPF2.

Miscellaneous databases

PROiQ9EPF2.

Gene expression databases

BgeeiENSRNOG00000007726.
GenevisibleiQ9EPF2. RN.

Family and domain databases

Gene3Di2.60.40.10. 5 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
PROSITEiPS50835. IG_LIKE. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMUC18_RAT
AccessioniPrimary (citable) accession number: Q9EPF2
Secondary accession number(s): Q6IRH8, Q9ESS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: November 2, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.