ID   GPR88_MOUSE             Reviewed;         384 AA.
AC   Q9EPB7;
DT   29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   27-MAR-2024, entry version 139.
DE   RecName: Full=G protein-coupled receptor 88 {ECO:0000305};
DE   AltName: Full=Striatum-specific G-protein coupled receptor;
GN   Name=Gpr88; Synonyms=Strg;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=11056049; DOI=10.1006/geno.2000.6340;
RA   Mizushima K., Miyamoto Y., Tsukahara F., Hirai M., Sakaki Y., Ito T.;
RT   "A novel G-protein-coupled receptor gene expressed in striatum.";
RL   Genomics 69:314-321(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=26188600; DOI=10.1016/j.biopsych.2015.05.020;
RA   Meirsman A.C., Le Merrer J., Pellissier L.P., Diaz J., Clesse D.,
RA   Kieffer B.L., Becker J.A.;
RT   "Mice lacking GPR88 show motor deficit, improved spatial learning, and low
RT   anxiety reversed by delta opioid antagonist.";
RL   Biol. Psychiatry 79:917-927(2016).
CC   -!- FUNCTION: Orphan G protein-coupled receptor implicated in a large
CC       repertoire of behavioral responses that engage motor activities,
CC       spatial learning, and emotional processing (By similarity). May play a
CC       role in the regulation of cognitive and motor function
CC       (PubMed:26188600). Couples with the heterotrimeric G protein complex of
CC       the G(i) subfamily, consisting of GNAI1, GNB1 and GNG2, thereby acting
CC       through a G(i)-mediated pathway (By similarity). Plays a role in the
CC       attenuation of D1 dopamine receptor (D1R)-mediated cAMP response in
CC       ciliated cells (By similarity). In on-ciliated cells, involved in the
CC       inhibition of the beta-2 adrenergic receptor (B2AR) response (By
CC       similarity). {ECO:0000250|UniProtKB:Q9ESP4,
CC       ECO:0000250|UniProtKB:Q9GZN0, ECO:0000269|PubMed:26188600}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9ESP4};
CC       Multi-pass membrane protein {ECO:0000255}. Cell projection, cilium
CC       membrane {ECO:0000250|UniProtKB:Q9GZN0}; Multi-pass membrane protein
CC       {ECO:0000255}. Cytoplasm {ECO:0000250|UniProtKB:Q9ESP4}. Nucleus
CC       {ECO:0000250|UniProtKB:Q9ESP4}. Note=Localized to cilia in ciliated
CC       cells; whereas in non-ciliated cells, distributed throughout the cell
CC       membrane (By similarity). During cortical lamination, subcellular
CC       location shifts, on the day of birth, from expression at the plasma
CC       membrane and in the cytoplasm to the nuclei of neurons. This
CC       intranuclear localization remains throughout adulthood.
CC       {ECO:0000250|UniProtKB:Q9ESP4, ECO:0000250|UniProtKB:Q9GZN0}.
CC   -!- TISSUE SPECIFICITY: Expressed predominantly in the striatum.
CC       {ECO:0000269|PubMed:11056049}.
CC   -!- DISRUPTION PHENOTYPE: Mice have increased locomotion, increased motor
CC       stereotypical behavior, and impaired motor skill learning. Mutant mice
CC       also show facilitated hippocampal-mediated behaviors and decreased
CC       anxiety. Chronic blockade of delta opioid receptors and mu opioid, but
CC       not other Gi/o coupled receptors, using delta opioid receptor
CC       antagonist partially improved motor coordination and normalized spatial
CC       navigation and anxiety of mutant mice. {ECO:0000269|PubMed:26188600}.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB042408; BAB18245.1; -; mRNA.
DR   EMBL; AB042409; BAB18246.1; -; Genomic_DNA.
DR   EMBL; BC051941; AAH51941.1; -; mRNA.
DR   CCDS; CCDS38610.1; -.
DR   RefSeq; NP_071872.1; NM_022427.2.
DR   RefSeq; XP_006501919.1; XM_006501856.1.
DR   RefSeq; XP_006501920.1; XM_006501857.1.
DR   AlphaFoldDB; Q9EPB7; -.
DR   SMR; Q9EPB7; -.
DR   STRING; 10090.ENSMUSP00000087959; -.
DR   BindingDB; Q9EPB7; -.
DR   ChEMBL; CHEMBL3879835; -.
DR   GlyCosmos; Q9EPB7; 1 site, No reported glycans.
DR   GlyGen; Q9EPB7; 1 site.
DR   PhosphoSitePlus; Q9EPB7; -.
DR   PaxDb; 10090-ENSMUSP00000087959; -.
DR   ProteomicsDB; 271072; -.
DR   Antibodypedia; 19999; 211 antibodies from 26 providers.
DR   DNASU; 64378; -.
DR   Ensembl; ENSMUST00000090473.7; ENSMUSP00000087959.6; ENSMUSG00000068696.7.
DR   GeneID; 64378; -.
DR   KEGG; mmu:64378; -.
DR   UCSC; uc008rbz.2; mouse.
DR   AGR; MGI:1927653; -.
DR   CTD; 54112; -.
DR   MGI; MGI:1927653; Gpr88.
DR   VEuPathDB; HostDB:ENSMUSG00000068696; -.
DR   eggNOG; KOG3656; Eukaryota.
DR   GeneTree; ENSGT00390000009609; -.
DR   HOGENOM; CLU_053532_0_0_1; -.
DR   InParanoid; Q9EPB7; -.
DR   OMA; LLYTWKN; -.
DR   OrthoDB; 5362232at2759; -.
DR   PhylomeDB; Q9EPB7; -.
DR   TreeFam; TF336499; -.
DR   BioGRID-ORCS; 64378; 2 hits in 76 CRISPR screens.
DR   PRO; PR:Q9EPB7; -.
DR   Proteomes; UP000000589; Chromosome 3.
DR   RNAct; Q9EPB7; Protein.
DR   Bgee; ENSMUSG00000068696; Expressed in caudate-putamen and 138 other cell types or tissues.
DR   ExpressionAtlas; Q9EPB7; baseline and differential.
DR   GO; GO:0060170; C:ciliary membrane; ISS:UniProtKB.
DR   GO; GO:0005929; C:cilium; ISO:MGI.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR   GO; GO:0004941; F:beta2-adrenergic receptor activity; ISS:UniProtKB.
DR   GO; GO:0003774; F:cytoskeletal motor activity; IMP:UniProtKB.
DR   GO; GO:0008020; F:G protein-coupled photoreceptor activity; IBA:GO_Central.
DR   GO; GO:0004930; F:G protein-coupled receptor activity; ISS:MGI.
DR   GO; GO:0004888; F:transmembrane signaling receptor activity; ISS:MGI.
DR   GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR   GO; GO:0071482; P:cellular response to light stimulus; IBA:GO_Central.
DR   GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR   GO; GO:0007626; P:locomotory behavior; IMP:MGI.
DR   GO; GO:0061743; P:motor learning; IMP:UniProtKB.
DR   GO; GO:0050885; P:neuromuscular process controlling balance; IMP:MGI.
DR   GO; GO:0019228; P:neuronal action potential; IMP:MGI.
DR   GO; GO:0007602; P:phototransduction; IBA:GO_Central.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR000276; GPCR_Rhodpsn.
DR   InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR   PANTHER; PTHR24240:SF165; G-PROTEIN COUPLED RECEPTOR 88-RELATED; 1.
DR   PANTHER; PTHR24240; OPSIN; 1.
DR   Pfam; PF00001; 7tm_1; 1.
DR   PRINTS; PR00237; GPCRRHODOPSN.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR   Genevisible; Q9EPB7; MM.
PE   2: Evidence at transcript level;
KW   Cell membrane; Cell projection; Cytoplasm; G-protein coupled receptor;
KW   Glycoprotein; Membrane; Nucleus; Receptor; Reference proteome; Transducer;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..384
FT                   /note="G protein-coupled receptor 88"
FT                   /id="PRO_0000069598"
FT   TOPO_DOM        1..35
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        36..56
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        57..73
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        74..94
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        95..116
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        117..136
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        137..158
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        159..179
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        180..195
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        196..216
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        217..285
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        286..306
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        307..310
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        311..331
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        332..384
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        3
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   384 AA;  40240 MW;  1E5BB8A5B5B3B37D CRC64;
     MTNSSSTSTS TTTGGSLLLL CEEEESWAGR RIPVSLLYSG LAIGGTLANG MVIYLVSSFR
     KLQTTSNAFI VNGCAADLSV CALWMPQEAV LGLLPSGSAE PPGDWDGGGG SYRLLRGGLL
     GLGLTVSLLS HCLVALNRYL LITRAPATYQ VLYQRRHTVG MLALSWALAL GLVLLLPPWA
     PKPGAEPPQV HYPALLAAGA LLAQTALLLH CYLGIVRRVR VSVKRVSVLN FHLLHQLPGC
     AAAAAAFPAA PHAPGPGGAA HPAQPQPLPA ALQPRRAQRR LSGLSVLLLC CVFLLATQPL
     VWVSLASGFS LPVPWGVQAA SWLLCCALSA LNPLLYTWRN EEFRRSVRSV LPGVGDAAAA
     AAAATAVPAM SQAQLGTRAA GQHW
//