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Protein

Neuropeptide FF receptor 1

Gene

Npffr1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Neuropeptide FF receptor 1
Alternative name(s):
G-protein coupled receptor 147
RFamide-related peptide receptor OT7T022
Gene namesi
Name:Npffr1
Synonyms:Gpr147, Npff1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621570. Npffr1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 43ExtracellularSequence analysisAdd BLAST43
Transmembranei44 – 64Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini65 – 80CytoplasmicSequence analysisAdd BLAST16
Transmembranei81 – 101Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini102 – 117ExtracellularSequence analysisAdd BLAST16
Transmembranei118 – 138Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini139 – 158CytoplasmicSequence analysisAdd BLAST20
Transmembranei159 – 179Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini180 – 214ExtracellularSequence analysisAdd BLAST35
Transmembranei215 – 235Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini236 – 273CytoplasmicSequence analysisAdd BLAST38
Transmembranei274 – 294Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini295 – 309ExtracellularSequence analysisAdd BLAST15
Transmembranei310 – 330Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini331 – 432CytoplasmicSequence analysisAdd BLAST102

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000699141 – 432Neuropeptide FF receptor 1Add BLAST432

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi10N-linked (GlcNAc...)Sequence analysis1
Glycosylationi18N-linked (GlcNAc...)Sequence analysis1
Glycosylationi113N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi116 ↔ 203PROSITE-ProRule annotation
Glycosylationi195N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9EP86.
PRIDEiQ9EP86.

PTM databases

PhosphoSitePlusiQ9EP86.

Expressioni

Tissue specificityi

Expressed at high levels in the hypothalamus. Moderate levels found in the midbrain, thalamus, medulla oblongata, testis, eye, whole brain, cerebral cortex, striatum, hippocampus, cerebellum, optic nerve, placenta, spinal cord, pituitary gland and ovary.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000675.

Structurei

3D structure databases

ProteinModelPortaliQ9EP86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
HOGENOMiHOG000041285.
HOVERGENiHBG099624.
InParanoidiQ9EP86.
PhylomeDBiQ9EP86.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR005395. NPFF_rcpt.
IPR005396. NPFF_rcpt_1.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01570. NPFFRECEPTOR.
PR01571. NPFFRECEPTR1.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EP86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAEPSQPPN GSWPLGQNGS DVETSMATSL TFSSYYQHSS PVAAMFIAAY
60 70 80 90 100
VLIFLLCMVG NTLVCFIVLK NRHMRTVTNM FILNLAVSDL LVGIFCMPTT
110 120 130 140 150
LVDNLITGWP FDNATCKMSG LVQGMSVSAS VFTLVAIAVE RFRCIVHPFR
160 170 180 190 200
EKLTLRKALF TIAVIWALAL LIMCPSAVTL TVTREEHHFM LDARNRSYPL
210 220 230 240 250
YSCWEAWPEK GMRKVYTAVL FAHIYLVPLA LIVVMYVRIA RKLCQAPGPA
260 270 280 290 300
RDTEEAVAEG GRTSRRRARV VHMLVMVALF FTLSWLPLWV LLLLIDYGEL
310 320 330 340 350
SELQLHLLSV YAFPLAHWLA FFHSSANPII YGYFNENFRR GFQAAFRAQL
360 370 380 390 400
CWPPWAAHKQ AYSERPNRLL RRRVVVDVQP SDSGLPSESG PSSGVPGPGR
410 420 430
LPLRNGRVAH QDGPGEGPGC NHMPLTIPAW NI
Length:432
Mass (Da):48,324
Last modified:March 1, 2001 - v1
Checksum:i827325849444C408
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040103 mRNA. Translation: BAB17676.1.
AF268901 mRNA. Translation: AAG41400.1.
UniGeneiRn.64483.

Genome annotation databases

UCSCiRGD:621570. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040103 mRNA. Translation: BAB17676.1.
AF268901 mRNA. Translation: AAG41400.1.
UniGeneiRn.64483.

3D structure databases

ProteinModelPortaliQ9EP86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000675.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSitePlusiQ9EP86.

Proteomic databases

PaxDbiQ9EP86.
PRIDEiQ9EP86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:621570. rat.

Organism-specific databases

RGDi621570. Npffr1.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
HOGENOMiHOG000041285.
HOVERGENiHBG099624.
InParanoidiQ9EP86.
PhylomeDBiQ9EP86.

Miscellaneous databases

PROiQ9EP86.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR005395. NPFF_rcpt.
IPR005396. NPFF_rcpt_1.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01570. NPFFRECEPTOR.
PR01571. NPFFRECEPTR1.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNPFF1_RAT
AccessioniPrimary (citable) accession number: Q9EP86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.