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Protein

Neuropeptide FF receptor 1

Gene

Npffr1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Neuropeptide FF receptor 1
Alternative name(s):
G-protein coupled receptor 147
RFamide-related peptide receptor OT7T022
Gene namesi
Name:Npffr1
Synonyms:Gpr147, Npff1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621570. Npffr1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4343ExtracellularSequence analysisAdd
BLAST
Transmembranei44 – 6421Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini65 – 8016CytoplasmicSequence analysisAdd
BLAST
Transmembranei81 – 10121Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini102 – 11716ExtracellularSequence analysisAdd
BLAST
Transmembranei118 – 13821Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini139 – 15820CytoplasmicSequence analysisAdd
BLAST
Transmembranei159 – 17921Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini180 – 21435ExtracellularSequence analysisAdd
BLAST
Transmembranei215 – 23521Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini236 – 27338CytoplasmicSequence analysisAdd
BLAST
Transmembranei274 – 29421Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini295 – 30915ExtracellularSequence analysisAdd
BLAST
Transmembranei310 – 33021Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini331 – 432102CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 432432Neuropeptide FF receptor 1PRO_0000069914Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi10 – 101N-linked (GlcNAc...)Sequence analysis
Glycosylationi18 – 181N-linked (GlcNAc...)Sequence analysis
Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence analysis
Disulfide bondi116 ↔ 203PROSITE-ProRule annotation
Glycosylationi195 – 1951N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9EP86.
PRIDEiQ9EP86.

PTM databases

PhosphoSiteiQ9EP86.

Expressioni

Tissue specificityi

Expressed at high levels in the hypothalamus. Moderate levels found in the midbrain, thalamus, medulla oblongata, testis, eye, whole brain, cerebral cortex, striatum, hippocampus, cerebellum, optic nerve, placenta, spinal cord, pituitary gland and ovary.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000675.

Structurei

3D structure databases

ProteinModelPortaliQ9EP86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
HOGENOMiHOG000041285.
HOVERGENiHBG099624.
InParanoidiQ9EP86.
PhylomeDBiQ9EP86.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR005395. NPFF_rcpt.
IPR005396. NPFF_rcpt_1.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01570. NPFFRECEPTOR.
PR01571. NPFFRECEPTR1.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EP86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAEPSQPPN GSWPLGQNGS DVETSMATSL TFSSYYQHSS PVAAMFIAAY
60 70 80 90 100
VLIFLLCMVG NTLVCFIVLK NRHMRTVTNM FILNLAVSDL LVGIFCMPTT
110 120 130 140 150
LVDNLITGWP FDNATCKMSG LVQGMSVSAS VFTLVAIAVE RFRCIVHPFR
160 170 180 190 200
EKLTLRKALF TIAVIWALAL LIMCPSAVTL TVTREEHHFM LDARNRSYPL
210 220 230 240 250
YSCWEAWPEK GMRKVYTAVL FAHIYLVPLA LIVVMYVRIA RKLCQAPGPA
260 270 280 290 300
RDTEEAVAEG GRTSRRRARV VHMLVMVALF FTLSWLPLWV LLLLIDYGEL
310 320 330 340 350
SELQLHLLSV YAFPLAHWLA FFHSSANPII YGYFNENFRR GFQAAFRAQL
360 370 380 390 400
CWPPWAAHKQ AYSERPNRLL RRRVVVDVQP SDSGLPSESG PSSGVPGPGR
410 420 430
LPLRNGRVAH QDGPGEGPGC NHMPLTIPAW NI
Length:432
Mass (Da):48,324
Last modified:March 1, 2001 - v1
Checksum:i827325849444C408
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040103 mRNA. Translation: BAB17676.1.
AF268901 mRNA. Translation: AAG41400.1.
UniGeneiRn.64483.

Genome annotation databases

UCSCiRGD:621570. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040103 mRNA. Translation: BAB17676.1.
AF268901 mRNA. Translation: AAG41400.1.
UniGeneiRn.64483.

3D structure databases

ProteinModelPortaliQ9EP86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000675.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ9EP86.

Proteomic databases

PaxDbiQ9EP86.
PRIDEiQ9EP86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:621570. rat.

Organism-specific databases

RGDi621570. Npffr1.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
HOGENOMiHOG000041285.
HOVERGENiHBG099624.
InParanoidiQ9EP86.
PhylomeDBiQ9EP86.

Miscellaneous databases

NextBioi13959885.
PROiQ9EP86.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR005395. NPFF_rcpt.
IPR005396. NPFF_rcpt_1.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01570. NPFFRECEPTOR.
PR01571. NPFFRECEPTR1.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Brain stem.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Hypothalamus.
  3. Lubec G., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 185-194, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiNPFF1_RAT
AccessioniPrimary (citable) accession number: Q9EP86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 1, 2001
Last modified: May 11, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.