Q9EP82Q80V01WDR4_MOUSEtRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4Protein Wuho homologmWHWD repeat-containing protein 4Wdr4Mus musculusMouseEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMusIsolation and characterization of a human chromosome 21q22.3 gene (WDR4) and its mouse homologue that code for a WD-repeat protein.NUCLEOTIDE SEQUENCE [MRNA]The transcriptional landscape of the mammalian genome.NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]A tissue-specific atlas of mouse protein phosphorylation and expression.IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]Wuho is a new member in maintaining genome stability through its interaction with flap endonuclease 1.DISRUPTION PHENOTYPEWuho/WDR4 deficiency inhibits cell proliferation and induces apoptosis via DNA damage in mouse embryonic fibroblasts.FUNCTIONMettl1/Wdr4-mediated m7G tRNA methylome is required for normal mRNA translation and embryonic stem cell self-renewal and differentiation.FUNCTIONPATHWAYNon-catalytic component of the METTL1-WDR4 methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:29983320). In the METTL1-WDR4 methyltransferase complex, WDR4 acts as a scaffold for tRNA-binding (By similarity). Required for the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop (PubMed:29983320). M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay (By similarity). Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs (By similarity). Also required for methylation of a specific subset of miRNAs, such as let-7 (By similarity). Acts as a regulator of embryonic stem cell self-renewal and differentiation (PubMed:29983320). Independently of METTL1, also plays a role in genome stability: localizes at the DNA replication site and regulates endonucleolytic activities of FEN1 (PubMed:29574139).tRNA modification; N(7)-methylguanine-tRNA biosynthesis.Non-catalytic component of the METTL1-WDR4 complex, composed of METTL1 and WDR4. Interacts with FEN1; the interaction is direct.NucleusChromosomeLocalizes at the site of nascent DNA synthesis.Embryonic lethality at E9.5-10.5, possibly caused by DNA damage.Belongs to the WD repeat TRM82 family.AcetylationChromosomeDNA damageNucleusPhosphoproteinReference proteomeRepeattRNA processingWD repeatTAMASSAGLALCAQTLVVRGGSRFLAFSTTGSDDDCVFTYDCSTAEKKATPEDKGEDGQPADTGSDSILASTFSKSGRYFALTDDSKRLILFRTKPWQCLSVRMVVRRCTALTFTASEDRVLVADKSGDVYSFSVLEPDGCGRLELGHLSMLLDVAVSPDDQFVLTADRDEKIRVSWAAAPHSIESFCLGHTEFVSRILVVPSHPELLLSSSGDGTLRLWEYRSGRQLQCCDLAGLQEPGEQPGHKGLAASRIAFWGQESYVVLLCECVPVVFVFQLDASRQQLVFRQRLTFPHRVWDVVFEEARGLWVLQDCRDAPLVLWRPVGGEWQAAPDGAVSPRLCSHLRESWAMLEGSVGTDDSFRSLYKATFDNMTSYLKKKEERLQQQLKKKRQRSPFPGSPEQTKKACPGQSALSC
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