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Q9EP82 (WDR4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase subunit WDR4
Alternative name(s):
WD repeat-containing protein 4
Gene names
Name:Wdr4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length413 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational change of the catalytic subunit By similarity.

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis.

Subunit structure

Forms a complex with METTL1 By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the WD repeat TRM82 family.

Contains 5 WD repeats.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   DomainRepeat
WD repeat
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA modification

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Compara

nucleoplasm

Inferred from electronic annotation. Source: Compara

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: Compara

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 413412tRNA (guanine-N(7)-)-methyltransferase subunit WDR4
PRO_0000051349

Regions

Repeat61 – 10040WD 1
Repeat102 – 14140WD 2
Repeat145 – 18541WD 3
Repeat188 – 22841WD 4
Repeat289 – 32941WD 5

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue3921Phosphoserine Ref.4
Modified residue4121Phosphoserine By similarity

Experimental info

Sequence conflict281T → A in CAC05584. Ref.1
Sequence conflict281T → A in CAC05585. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9EP82 [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: 8EAFB152CFE2884E

FASTA41345,756
        10         20         30         40         50         60 
MASSAGLALC AQTLVVRGGS RFLAFSTTGS DDDCVFTYDC STAEKKATPE DKGEDGQPAD 

        70         80         90        100        110        120 
TGSDSILAST FSKSGRYFAL TDDSKRLILF RTKPWQCLSV RMVVRRCTAL TFTASEDRVL 

       130        140        150        160        170        180 
VADKSGDVYS FSVLEPDGCG RLELGHLSML LDVAVSPDDQ FVLTADRDEK IRVSWAAAPH 

       190        200        210        220        230        240 
SIESFCLGHT EFVSRILVVP SHPELLLSSS GDGTLRLWEY RSGRQLQCCD LAGLQEPGEQ 

       250        260        270        280        290        300 
PGHKGLAASR IAFWGQESYV VLLCECVPVV FVFQLDASRQ QLVFRQRLTF PHRVWDVVFE 

       310        320        330        340        350        360 
EARGLWVLQD CRDAPLVLWR PVGGEWQAAP DGAVSPRLCS HLRESWAMLE GSVGTDDSFR 

       370        380        390        400        410 
SLYKATFDNM TSYLKKKEER LQQQLKKKRQ RSPFPGSPEQ TKKACPGQSA LSC

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of a human chromosome 21q22.3 gene (WDR4) and its mouse homologue that code for a WD-repeat protein."
Michaud J., Kudoh J., Berry A., Bonne-Tamir B., Lalioti M.D., Rossier C., Shibuya K., Kawasaki K., Asakawa S., Minoshima S., Shimizu N., Antonarakis S.E., Scott H.S.
Genomics 68:71-79(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Bone marrow and Lung.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-392, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ271893 mRNA. Translation: CAC05585.1.
AJ271892 mRNA. Translation: CAC05584.1.
AK144821 mRNA. Translation: BAE26082.1.
AK152271 mRNA. Translation: BAE31087.1.
AK153161 mRNA. Translation: BAE31771.1.
BC039272 mRNA. Translation: AAH39272.1.
IPIIPI00985611.
RefSeqNP_067297.2. NM_021322.2.
UniGeneMm.143771.

3D structure databases

ProteinModelPortalQ9EP82.
SMRQ9EP82. Positions 72-294.
ModBaseSearch...

PTM databases

PhosphoSiteQ9EP82.

Proteomic databases

PaxDbQ9EP82.
PRIDEQ9EP82.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID57773.
KEGGmmu:57773.
UCSCuc008bvc.2. mouse.

Organism-specific databases

CTD10785.
MGIMGI:1889002. Wdr4.

Phylogenomic databases

eggNOGCOG2319.
HOGENOMHOG000154849.
HOVERGENHBG022880.
InParanoidQ80V01.
KOK15443.
OrthoDBEOG4G1MGZ.

Enzyme and pathway databases

UniPathwayUPA00989.

Gene expression databases

ArrayExpressQ9EP82.
BgeeQ9EP82.
CleanExMM_WDR4.
GenevestigatorQ9EP82.
GermOnlineENSMUSG00000024037. Mus musculus.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 2 hits.
[Graphical view]
SMARTSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMSSF50978. WD40_like. 1 hit.
PROSITEPS00678. WD_REPEATS_1. False negative.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio313940.
SOURCESearch...

Entry information

Entry nameWDR4_MOUSE
AccessionPrimary (citable) accession number: Q9EP82
Secondary accession number(s): Q80V01
Entry history
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: May 5, 2009
Last modified: May 1, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents