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Q9EP82

- WDR4_MOUSE

UniProt

Q9EP82 - WDR4_MOUSE

Protein

tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4

Gene

Wdr4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (05 May 2009)
      Previous versions | rss
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    Functioni

    Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit.UniRule annotation

    Pathwayi

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: Ensembl

    GO - Biological processi

    1. tRNA modification Source: UniProtKB

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4UniRule annotation
    Alternative name(s):
    WD repeat-containing protein 4UniRule annotation
    Gene namesi
    Name:Wdr4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1889002. Wdr4.

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nucleoplasm Source: Ensembl
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 413412tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4PRO_0000051349Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei392 – 3921PhosphoserineBy similarity
    Modified residuei412 – 4121PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ9EP82.
    PRIDEiQ9EP82.

    PTM databases

    PhosphoSiteiQ9EP82.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9EP82.
    BgeeiQ9EP82.
    CleanExiMM_WDR4.
    GenevestigatoriQ9EP82.

    Interactioni

    Subunit structurei

    Forms a heterodimer with the catalytic subunit METTL1.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ9EP82.
    SMRiQ9EP82. Positions 72-294.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati61 – 10040WD 1Add
    BLAST
    Repeati102 – 14140WD 2Add
    BLAST
    Repeati145 – 18541WD 3Add
    BLAST
    Repeati188 – 22841WD 4Add
    BLAST
    Repeati289 – 32941WD 5Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat TRM82 family.UniRule annotation
    Contains 5 WD repeats.UniRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    HOGENOMiHOG000154849.
    HOVERGENiHBG022880.
    InParanoidiQ80V01.
    KOiK15443.
    PhylomeDBiQ9EP82.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    HAMAPiMF_03056. TRM82.
    InterProiIPR028884. Trm82.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 2 hits.
    [Graphical view]
    SMARTiSM00320. WD40. 4 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9EP82-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASSAGLALC AQTLVVRGGS RFLAFSTTGS DDDCVFTYDC STAEKKATPE    50
    DKGEDGQPAD TGSDSILAST FSKSGRYFAL TDDSKRLILF RTKPWQCLSV 100
    RMVVRRCTAL TFTASEDRVL VADKSGDVYS FSVLEPDGCG RLELGHLSML 150
    LDVAVSPDDQ FVLTADRDEK IRVSWAAAPH SIESFCLGHT EFVSRILVVP 200
    SHPELLLSSS GDGTLRLWEY RSGRQLQCCD LAGLQEPGEQ PGHKGLAASR 250
    IAFWGQESYV VLLCECVPVV FVFQLDASRQ QLVFRQRLTF PHRVWDVVFE 300
    EARGLWVLQD CRDAPLVLWR PVGGEWQAAP DGAVSPRLCS HLRESWAMLE 350
    GSVGTDDSFR SLYKATFDNM TSYLKKKEER LQQQLKKKRQ RSPFPGSPEQ 400
    TKKACPGQSA LSC 413
    Length:413
    Mass (Da):45,756
    Last modified:May 5, 2009 - v2
    Checksum:i8EAFB152CFE2884E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 281T → A in CAC05584. (PubMed:10950928)Curated
    Sequence conflicti28 – 281T → A in CAC05585. (PubMed:10950928)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ271893 mRNA. Translation: CAC05585.1.
    AJ271892 mRNA. Translation: CAC05584.1.
    AK144821 mRNA. Translation: BAE26082.1.
    AK152271 mRNA. Translation: BAE31087.1.
    AK153161 mRNA. Translation: BAE31771.1.
    BC039272 mRNA. Translation: AAH39272.1.
    RefSeqiNP_067297.2. NM_021322.2.
    UniGeneiMm.143771.

    Genome annotation databases

    GeneIDi57773.
    KEGGimmu:57773.
    UCSCiuc008bvd.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ271893 mRNA. Translation: CAC05585.1 .
    AJ271892 mRNA. Translation: CAC05584.1 .
    AK144821 mRNA. Translation: BAE26082.1 .
    AK152271 mRNA. Translation: BAE31087.1 .
    AK153161 mRNA. Translation: BAE31771.1 .
    BC039272 mRNA. Translation: AAH39272.1 .
    RefSeqi NP_067297.2. NM_021322.2.
    UniGenei Mm.143771.

    3D structure databases

    ProteinModelPortali Q9EP82.
    SMRi Q9EP82. Positions 72-294.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9EP82.

    Proteomic databases

    PaxDbi Q9EP82.
    PRIDEi Q9EP82.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 57773.
    KEGGi mmu:57773.
    UCSCi uc008bvd.2. mouse.

    Organism-specific databases

    CTDi 10785.
    MGIi MGI:1889002. Wdr4.

    Phylogenomic databases

    eggNOGi COG2319.
    HOGENOMi HOG000154849.
    HOVERGENi HBG022880.
    InParanoidi Q80V01.
    KOi K15443.
    PhylomeDBi Q9EP82.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Miscellaneous databases

    NextBioi 313940.
    PROi Q9EP82.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9EP82.
    Bgeei Q9EP82.
    CleanExi MM_WDR4.
    Genevestigatori Q9EP82.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    HAMAPi MF_03056. TRM82.
    InterProi IPR028884. Trm82.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 2 hits.
    [Graphical view ]
    SMARTi SM00320. WD40. 4 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of a human chromosome 21q22.3 gene (WDR4) and its mouse homologue that code for a WD-repeat protein."
      Michaud J., Kudoh J., Berry A., Bonne-Tamir B., Lalioti M.D., Rossier C., Shibuya K., Kawasaki K., Asakawa S., Minoshima S., Shimizu N., Antonarakis S.E., Scott H.S.
      Genomics 68:71-79(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Bone marrow and Lung.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiWDR4_MOUSE
    AccessioniPrimary (citable) accession number: Q9EP82
    Secondary accession number(s): Q80V01
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 11, 2002
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3