Carbohydrate sulfotransferase 7 - Mus musculus (Mouse)

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Q9EP78 (CHST7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 82. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Carbohydrate sulfotransferase 7
EC=2.8.2.-
EC=2.8.2.17

Alternative name(s):
Chondroitin 6-sulfotransferase 2
Short name=C6ST-2
Short name=mC6ST-2
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 5
Short name=GST-5
N-acetylglucosamine 6-O-sulfotransferase 4
Short name=GlcNAc6ST-4
Short name=Gn6st-4

Gene names

Name:	Chst7
Synonyms:	Gst5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. Preferentially acts on mannose-linked GlcNAc. Also able to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Also acts on core 2 mucin-type oligosaccharide and N-acetyllactosamine oligomer with a lower efficiency. Has weak or no activity toward keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc. Catalyzes 6-O-sulfation of beta-benzyl GlcNAc but not alpha- or beta-benzyl GalNAc. Ref.1
Catalytic activity	3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	Widely expressed. Highly expressed in kidney. Expressed at lower level in heart, lung and liver. Ref.1
Sequence similarities	Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-acetylglucosamine metabolic process Inferred from electronic annotation. Source: Compara carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW chondroitin sulfate biosynthetic process Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	N-acetylglucosamine 6-O-sulfotransferase activity Inferred from electronic annotation. Source: Compara chondroitin 6-sulfotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 484

484

Carbohydrate sulfotransferase 7

PRO_0000085199

Regions

Topological domain

1 – 12

Cytoplasmic Potential

Transmembrane

13 – 33

Helical; Signal-anchor for type II membrane protein; Potential

Topological domain

34 – 484

451

Lumenal Potential

Nucleotide binding

108 – 114

PAPS By similarity

Nucleotide binding

276 – 284

PAPS By similarity

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

184

N-linked (GlcNAc...) Potential

Glycosylation

405

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

E → K in BAB40372. Ref.3

Sequence conflict

130

E → D in BAB40372. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q9EP78 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 9B195537D7AB7193
Show »

FASTA

484

54,767

        10         20         30         40         50         60 
MKGRRRRRRE YCKFTLLLAL YTLLLLLVPS VLDSHSEQDK GRNCPGLQRS LGVWSLEAAA 

        70         80         90        100        110        120 
AGEREQGAEV RSLAEGNPDR SPGSPGNLSA VGEAVTQEKQ HIYVHATWRT GSSFLGELFN 

       130        140        150        160        170        180 
QHPDVFYLYE PMWHLWQALY PGDAESLQGA LRDMLRSLFR CDFSVLRLYA QPGDPGERAP 

       190        200        210        220        230        240 
DSANLTTAML FRWRTNKVIC SPPLCPAAPR ARADVGLVED KACESTCPPV SLRALEAECR 

       250        260        270        280        290        300 
KYPVVVIKDV RLLDLGVLVP LLRDPGLNLK VVQLFRDPRA VHNSRLKSRQ GLLRESIQVL 

       310        320        330        340        350        360 
RTRQRGDHFH RVLLAHGVDA RPGGQARALP SAPRADFFLT SALEVICEAW LRDLLFTRGA 

       370        380        390        400        410        420 
PAWLRRRYLR LRYEDLVWQP QAQLRRLLRF SGLRTLAALD AFAFNMTRGS AYGADRPFHL 

       430        440        450        460        470        480 
SARDAREAVH AWRERLSQEQ VRQVETACAP AMRLLAYPRS GDERDRKTVR EGETPLETKA 


NWAV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Diversity of N-acetylglucosamine-6-O-sulfotransferases: molecular cloning of a novel enzyme with different distribution and specificities." Uchimura K., Fasakhany F., Kadomatsu K., Matsukawa T., Yamakawa T., Kurosawa N., Muramatsu T. Biochem. Biophys. Res. Commun. 274:291-296(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Brain.
[2]	"Sulfation of N-acetylglucosamine by chondroitin 6-sulfotransferase 2 (GST-5)." Bhakta S., Bartes A., Bowman K.G., Kao W.-M., Polsky I., Lee J.-K., Cook B.N., Bruehl R.E., Rosen S.D., Bertozzi C.R., Hemmerich S. J. Biol. Chem. 275:40226-40234(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6.
[3]	"Cloning and expression of mouse chondroitin 6-sulfotransferase-2." Kitagawa H., Uyama T., Sugahara K. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mammary gland.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB040710 mRNA. Translation: BAB13769.1. AF280089 Genomic DNA. Translation: AAG48247.1. AB046929 mRNA. Translation: BAB40372.1. AK011202 mRNA. Translation: BAB27465.1. BC019204 mRNA. Translation: AAH19204.1.
IPI	IPI00321101.
PIR	JC7350.
RefSeq	NP_068361.1. NM_021715.1.
UniGene	Mm.44827.
3D structure databases
ProteinModelPortal	Q9EP78.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000043222.
PTM databases
PhosphoSite	Q9EP78.
Proteomic databases
PRIDE	Q9EP78.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000044138; ENSMUSP00000043222; ENSMUSG00000037347.
GeneID	60322.
KEGG	mmu:60322.
Organism-specific databases
CTD	56548.
MGI	MGI:1891767. Chst7.
Phylogenomic databases
eggNOG	NOG71363.
GeneTree	ENSGT00530000062902.
HOGENOM	HOG000261614.
HOVERGEN	HBG050949.
InParanoid	Q9EP78.
KO	K04743.
OrthoDB	EOG4TQM96.
Enzyme and pathway databases
BRENDA	2.8.2.17. 3474.
Gene expression databases
ArrayExpress	Q9EP78.
Bgee	Q9EP78.
Genevestigator	Q9EP78.
GermOnline	ENSMUSG00000037347. Mus musculus.
Family and domain databases
InterPro	IPR016469. Carbohydrate_sulfotransferase. IPR000863. Sulfotransferase_dom. [Graphical view]
Pfam	PF00685. Sulfotransfer_1. 1 hit. [Graphical view]
PIRSF	PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
ProtoNet	Search...
Other
NextBio	314817.
SOURCE	Search...

Entry information

Entry name

CHST7_MOUSE

Accession

Primary (citable) accession number: Q9EP78
Secondary accession number(s): Q99NB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents