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Protein

Leukotriene-B4 omega-hydroxylase 3

Gene

Cyp4f14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. This enzyme requires molecular oxygen and NADPH for the omega-hydroxylation of LTB4 (By similarity).By similarity

Catalytic activityi

(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2 = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O.

Cofactori

hemeBy similarity

Pathwayi: leukotriene B4 degradation

This protein is involved in the pathway leukotriene B4 degradation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway leukotriene B4 degradation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei328Heme (covalent; via 1 link)By similarity1
Metal bindingi468Iron (heme axial ligand)By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.30 3474
ReactomeiR-MMU-211935 Fatty acids
R-MMU-211958 Miscellaneous substrates
R-MMU-211979 Eicosanoids
R-MMU-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-MMU-2142816 Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE)
UniPathwayiUPA00883

Names & Taxonomyi

Protein namesi
Recommended name:
Leukotriene-B4 omega-hydroxylase 3 (EC:1.14.13.30)
Alternative name(s):
Cyp4f-14
Cytochrome P450 4F14
Cytochrome P450-LTB-omega
Leukotriene-B4 20-monooxygenase 3
Gene namesi
Name:Cyp4f14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1927669 Cyp4f14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000518581 – 524Leukotriene-B4 omega-hydroxylase 3Add BLAST524

Proteomic databases

MaxQBiQ9EP75
PaxDbiQ9EP75
PeptideAtlasiQ9EP75
PRIDEiQ9EP75

PTM databases

iPTMnetiQ9EP75
PhosphoSitePlusiQ9EP75
SwissPalmiQ9EP75

Expressioni

Gene expression databases

BgeeiENSMUSG00000024292
GenevisibleiQ9EP75 MM

Interactioni

Protein-protein interaction databases

IntActiQ9EP75, 1 interactor
MINTiQ9EP75
STRINGi10090.ENSMUSP00000050478

Structurei

3D structure databases

ProteinModelPortaliQ9EP75
SMRiQ9EP75
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0157 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00900000140829
HOGENOMiHOG000233833
HOVERGENiHBG000182
InParanoidiQ9EP75
KOiK17726
OMAiNYLPRIT
OrthoDBiEOG091G06KN
PhylomeDBiQ9EP75
TreeFamiTF105088

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

Q9EP75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQLSLSWLG LGPEVAFPWK TLLLLGASWI LARILIQIYA AYRNYRHLHG
60 70 80 90 100
FPQPPKRNWL MGHVGMVTPT EQGLKELTRL VGTYPQGFLM WIGPMVPVIT
110 120 130 140 150
LCHSDIVRSI LNASAAVALK DVIFYSILKP WLGDGLLVSA GDKWSRHRRM
160 170 180 190 200
LTPAFHFNIL KPYVKIFNDS TNIMHAKWQR LISDGSARLD MFEHVSLMTL
210 220 230 240 250
DSLQKCVFSF DSNCQEKSSE YIAAILELSA LVAKRHQQPL MFMDLLYNLT
260 270 280 290 300
PDGMRFRKAC NVVHEFTDAV IRERHRTLPD QGLDDFLKSK AKSKTLDFID
310 320 330 340 350
VLLLSKDEDG KELSDEDIRA EADTFMFEGH DTTASGLSWI LYNLARHPEY
360 370 380 390 400
QERCRQEVQE LLRGREPEEI EWDDLAQLPF LTMCIKESLR LHPPVTVISR
410 420 430 440 450
CCTQDILLPD GRTIPKGIIC LISIFGIHHN PSVWPDPEVY DPFRFDPENI
460 470 480 490 500
KDSSPLAFIP FSAGPRNCIG QTFAMSEMKV ALALTLLRFR LLPDDKEPRR
510 520
QPELILRAEG GLWLRVEPLS AGAH
Length:524
Mass (Da):59,800
Last modified:March 1, 2001 - v1
Checksum:iA89F80E10925E9F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB037541 mRNA Translation: BAB12564.1
AB037540 mRNA Translation: BAB12563.1
AF233644 mRNA Translation: AAK15010.1
AK005007 mRNA Translation: BAB23740.1
AK018676 mRNA Translation: BAB31338.1
BC011228 mRNA Translation: AAH11228.1
BC094016 mRNA Translation: AAH94016.1
CCDSiCCDS28621.1
RefSeqiNP_001191262.1, NM_001204333.1
NP_001191263.1, NM_001204334.1
NP_001191264.1, NM_001204335.1
NP_001191265.1, NM_001204336.1
NP_071879.1, NM_022434.2
XP_006524845.1, XM_006524782.3
UniGeneiMm.486449

Genome annotation databases

EnsembliENSMUST00000054174; ENSMUSP00000050478; ENSMUSG00000024292
ENSMUST00000179434; ENSMUSP00000136139; ENSMUSG00000024292
GeneIDi64385
KEGGimmu:64385
UCSCiuc008bxq.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCP4FE_MOUSE
AccessioniPrimary (citable) accession number: Q9EP75
Secondary accession number(s): Q52L93
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: March 1, 2001
Last modified: March 28, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health