Reviewed, UniProtKB/Swiss-Prot Q9EP75 (CP4FE_MOUSE)

Names and origin

Protein names

Recommended name:
    Leukotriene-B4 omega-hydroxylase 3
    EC=1.14.13.30
Alternative name(s):
    Cytochrome P450 4F14
    Leukotriene-B4 20-monooxygenase 3
    Cytochrome P450-LTB-omega
    Cyp4f-14

Gene names

Name:

Cyp4f14

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. This enzyme requires molecular oxygen and NADPH for the omega-hydroxylation of LTB4 By similarity.
Catalytic activity	(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2 = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; leukotriene B4 degradation.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Microsome membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro leukotriene-B4 20-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	Leukotriene-B4 omega-hydroxylase 3		PRO_0000051858
Sites
	Metal binding	468	1	Iron (heme axial ligand) By similarity
	Binding site	328	1	Heme (covalent; via 1 link) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9EP75-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: A89F80E10925E9F4	FASTA	524	59,800
	10 20 30 40 50 60 MSQLSLSWLG LGPEVAFPWK TLLLLGASWI LARILIQIYA AYRNYRHLHG FPQPPKRNWL 70 80 90 100 110 120 MGHVGMVTPT EQGLKELTRL VGTYPQGFLM WIGPMVPVIT LCHSDIVRSI LNASAAVALK 130 140 150 160 170 180 DVIFYSILKP WLGDGLLVSA GDKWSRHRRM LTPAFHFNIL KPYVKIFNDS TNIMHAKWQR 190 200 210 220 230 240 LISDGSARLD MFEHVSLMTL DSLQKCVFSF DSNCQEKSSE YIAAILELSA LVAKRHQQPL 250 260 270 280 290 300 MFMDLLYNLT PDGMRFRKAC NVVHEFTDAV IRERHRTLPD QGLDDFLKSK AKSKTLDFID 310 320 330 340 350 360 VLLLSKDEDG KELSDEDIRA EADTFMFEGH DTTASGLSWI LYNLARHPEY QERCRQEVQE 370 380 390 400 410 420 LLRGREPEEI EWDDLAQLPF LTMCIKESLR LHPPVTVISR CCTQDILLPD GRTIPKGIIC 430 440 450 460 470 480 LISIFGIHHN PSVWPDPEVY DPFRFDPENI KDSSPLAFIP FSAGPRNCIG QTFAMSEMKV 490 500 510 520 ALALTLLRFR LLPDDKEPRR QPELILRAEG GLWLRVEPLS AGAH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse liver leukotriene B4 omega-hydroxylase." Kikuta Y., Kasyu H., Kusunose E., Kusunose M. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Liver.
[2]	"Protein expression and catalytic activity assessment of mouse 4F clones." Antonovic L., Kawashima H., Strobel H. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129/Sv. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Cecum and Liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB037541 mRNA. Translation: BAB12564.1. AB037540 mRNA. Translation: BAB12563.1. AF233644 mRNA. Translation: AAK15010.1. AK005007 mRNA. Translation: BAB23740.1. AK018676 mRNA. Translation: BAB31338.1. BC011228 mRNA. Translation: AAH11228.1. BC094016 mRNA. Translation: AAH94016.1.
IPI	IPI00109257.
RefSeq	NP_071879.1.
UniGene	Mm.426027
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9EP75.
Proteomic databases
PRIDE	Q9EP75.
Genome annotation databases
Ensembl	ENSMUST00000054174; ENSMUSP00000050478; ENSMUSG00000024292; Mus musculus. [Genome view]
GeneID	64385.
KEGG	mmu:64385.
UCSC	uc008bxq.1. mouse.
Organism-specific databases
CTD	64385.
MGI	MGI:1927669. Cyp4f14.
Phylogenomic databases
HOGENOM	Q9EP75.
HOVERGEN	Q9EP75.
OMA	ERHRTLP.
Enzyme and pathway databases
BRENDA	1.14.13.30. 244.
Gene expression databases
ArrayExpress	Q9EP75.
Bgee	Q9EP75.
Genevestigator	Q9EP75.
GermOnline	ENSMUSG00000024292. Mus musculus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	320067.
SOURCE	Search...

Entry information

Entry name

CP4FE_MOUSE

Accession

Primary (citable) accession number: Q9EP75
Secondary accession number(s): Q52L93

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)