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Protein

Leukotriene-B4 omega-hydroxylase 3

Gene

Cyp4f14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. This enzyme requires molecular oxygen and NADPH for the omega-hydroxylation of LTB4 (By similarity).By similarity

Catalytic activityi

(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2 = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O.

Cofactori

hemeBy similarity

Pathwayi: leukotriene B4 degradation

This protein is involved in the pathway leukotriene B4 degradation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway leukotriene B4 degradation and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei328 – 3281Heme (covalent; via 1 link)By similarity
Metal bindingi468 – 4681Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.30. 3474.
ReactomeiR-MMU-211935. Fatty acids.
R-MMU-211958. Miscellaneous substrates.
R-MMU-211979. Eicosanoids.
R-MMU-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-MMU-2142816. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
UniPathwayiUPA00883.

Names & Taxonomyi

Protein namesi
Recommended name:
Leukotriene-B4 omega-hydroxylase 3 (EC:1.14.13.30)
Alternative name(s):
Cyp4f-14
Cytochrome P450 4F14
Cytochrome P450-LTB-omega
Leukotriene-B4 20-monooxygenase 3
Gene namesi
Name:Cyp4f14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1927669. Cyp4f14.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 524524Leukotriene-B4 omega-hydroxylase 3PRO_0000051858Add
BLAST

Proteomic databases

EPDiQ9EP75.
MaxQBiQ9EP75.
PaxDbiQ9EP75.
PRIDEiQ9EP75.

PTM databases

iPTMnetiQ9EP75.
PhosphoSiteiQ9EP75.
SwissPalmiQ9EP75.

Expressioni

Gene expression databases

BgeeiQ9EP75.
GenevisibleiQ9EP75. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9EP75. 1 interaction.
MINTiMINT-1862770.
STRINGi10090.ENSMUSP00000050478.

Structurei

3D structure databases

ProteinModelPortaliQ9EP75.
SMRiQ9EP75. Positions 97-519.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiQ9EP75.
KOiK17726.
OMAiDKEPRRQ.
OrthoDBiEOG7CNZFK.
PhylomeDBiQ9EP75.
TreeFamiTF105088.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EP75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQLSLSWLG LGPEVAFPWK TLLLLGASWI LARILIQIYA AYRNYRHLHG
60 70 80 90 100
FPQPPKRNWL MGHVGMVTPT EQGLKELTRL VGTYPQGFLM WIGPMVPVIT
110 120 130 140 150
LCHSDIVRSI LNASAAVALK DVIFYSILKP WLGDGLLVSA GDKWSRHRRM
160 170 180 190 200
LTPAFHFNIL KPYVKIFNDS TNIMHAKWQR LISDGSARLD MFEHVSLMTL
210 220 230 240 250
DSLQKCVFSF DSNCQEKSSE YIAAILELSA LVAKRHQQPL MFMDLLYNLT
260 270 280 290 300
PDGMRFRKAC NVVHEFTDAV IRERHRTLPD QGLDDFLKSK AKSKTLDFID
310 320 330 340 350
VLLLSKDEDG KELSDEDIRA EADTFMFEGH DTTASGLSWI LYNLARHPEY
360 370 380 390 400
QERCRQEVQE LLRGREPEEI EWDDLAQLPF LTMCIKESLR LHPPVTVISR
410 420 430 440 450
CCTQDILLPD GRTIPKGIIC LISIFGIHHN PSVWPDPEVY DPFRFDPENI
460 470 480 490 500
KDSSPLAFIP FSAGPRNCIG QTFAMSEMKV ALALTLLRFR LLPDDKEPRR
510 520
QPELILRAEG GLWLRVEPLS AGAH
Length:524
Mass (Da):59,800
Last modified:March 1, 2001 - v1
Checksum:iA89F80E10925E9F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB037541 mRNA. Translation: BAB12564.1.
AB037540 mRNA. Translation: BAB12563.1.
AF233644 mRNA. Translation: AAK15010.1.
AK005007 mRNA. Translation: BAB23740.1.
AK018676 mRNA. Translation: BAB31338.1.
BC011228 mRNA. Translation: AAH11228.1.
BC094016 mRNA. Translation: AAH94016.1.
CCDSiCCDS28621.1.
RefSeqiNP_001191262.1. NM_001204333.1.
NP_001191263.1. NM_001204334.1.
NP_001191264.1. NM_001204335.1.
NP_001191265.1. NM_001204336.1.
NP_071879.1. NM_022434.2.
XP_006524845.1. XM_006524782.2.
UniGeneiMm.486449.

Genome annotation databases

EnsembliENSMUST00000054174; ENSMUSP00000050478; ENSMUSG00000024292.
ENSMUST00000179434; ENSMUSP00000136139; ENSMUSG00000024292.
GeneIDi64385.
KEGGimmu:64385.
UCSCiuc008bxq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB037541 mRNA. Translation: BAB12564.1.
AB037540 mRNA. Translation: BAB12563.1.
AF233644 mRNA. Translation: AAK15010.1.
AK005007 mRNA. Translation: BAB23740.1.
AK018676 mRNA. Translation: BAB31338.1.
BC011228 mRNA. Translation: AAH11228.1.
BC094016 mRNA. Translation: AAH94016.1.
CCDSiCCDS28621.1.
RefSeqiNP_001191262.1. NM_001204333.1.
NP_001191263.1. NM_001204334.1.
NP_001191264.1. NM_001204335.1.
NP_001191265.1. NM_001204336.1.
NP_071879.1. NM_022434.2.
XP_006524845.1. XM_006524782.2.
UniGeneiMm.486449.

3D structure databases

ProteinModelPortaliQ9EP75.
SMRiQ9EP75. Positions 97-519.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9EP75. 1 interaction.
MINTiMINT-1862770.
STRINGi10090.ENSMUSP00000050478.

PTM databases

iPTMnetiQ9EP75.
PhosphoSiteiQ9EP75.
SwissPalmiQ9EP75.

Proteomic databases

EPDiQ9EP75.
MaxQBiQ9EP75.
PaxDbiQ9EP75.
PRIDEiQ9EP75.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054174; ENSMUSP00000050478; ENSMUSG00000024292.
ENSMUST00000179434; ENSMUSP00000136139; ENSMUSG00000024292.
GeneIDi64385.
KEGGimmu:64385.
UCSCiuc008bxq.2. mouse.

Organism-specific databases

CTDi64385.
MGIiMGI:1927669. Cyp4f14.

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiQ9EP75.
KOiK17726.
OMAiDKEPRRQ.
OrthoDBiEOG7CNZFK.
PhylomeDBiQ9EP75.
TreeFamiTF105088.

Enzyme and pathway databases

UniPathwayiUPA00883.
BRENDAi1.14.13.30. 3474.
ReactomeiR-MMU-211935. Fatty acids.
R-MMU-211958. Miscellaneous substrates.
R-MMU-211979. Eicosanoids.
R-MMU-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-MMU-2142816. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

Miscellaneous databases

ChiTaRSiCyp4f14. mouse.
PROiQ9EP75.
SOURCEiSearch...

Gene expression databases

BgeeiQ9EP75.
GenevisibleiQ9EP75. MM.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse liver leukotriene B4 omega-hydroxylase."
    Kikuta Y., Kasyu H., Kusunose E., Kusunose M.
    Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Liver.
  2. "Protein expression and catalytic activity assessment of mouse 4F clones."
    Antonovic L., Kawashima H., Strobel H.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129/Sv.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cecum and Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiCP4FE_MOUSE
AccessioniPrimary (citable) accession number: Q9EP75
Secondary accession number(s): Q52L93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: March 1, 2001
Last modified: June 8, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.