SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9EP73

- PD1L1_MOUSE

UniProt

Q9EP73 - PD1L1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Programmed cell death 1 ligand 1

Gene
Cd274, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the costimulatory signal essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.3 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. cell surface receptor signaling pathway Source: Ensembl
  2. immune response Source: Ensembl
  3. negative regulation of T cell proliferation Source: MGI
  4. positive regulation of interleukin-10 secretion Source: Ensembl
  5. T cell costimulation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Programmed cell death 1 ligand 1
Short name:
PD-L1
Short name:
PDCD1 ligand 1
Short name:
Programmed death ligand 1
Alternative name(s):
B7 homolog 1
Short name:
B7-H1
CD_antigen: CD274
Gene namesi
Name:Cd274
Synonyms:B7h1, Pdcd1l1, Pdcd1lg1, Pdl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:1926446. Cd274.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 239221Extracellular Reviewed predictionAdd
BLAST
Transmembranei240 – 26021Helical; Reviewed predictionAdd
BLAST
Topological domaini261 – 29030Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. external side of plasma membrane Source: MGI
  2. extracellular vesicular exosome Source: Ensembl
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi27 – 271L → A: PDCD1 binding. 1 Publication
Mutagenesisi31 – 311E → S: Significantly reduces the binding to PDCD1. 1 Publication
Mutagenesisi34 – 341S → Y: No effect on PDCD1 binding. 1 Publication
Mutagenesisi37 – 371T → Y: Significantly reduces the binding to PDCD1. 1 Publication
Mutagenesisi49 – 491D → S: No effect on PDCD1 binding. 1 Publication
Mutagenesisi56 – 561Y → S: No effect on PDCD1 binding. 1 Publication
Mutagenesisi58 – 581E → S: No effect on PDCD1 binding. 1 Publication
Mutagenesisi62 – 621E → S: No effect on PDCD1 binding. 1 Publication
Mutagenesisi67 – 671F → A: Abolishes the binding to PDCD1. Costimulates proliferation and IFNG production of T-cells. 1 Publication
Mutagenesisi69 – 691A → F: No effect on PDCD1 binding. 1 Publication
Mutagenesisi72 – 721E → S: No effect on PDCD1 binding. 1 Publication
Mutagenesisi75 – 751K → S: No effect on PDCD1 binding. 1 Publication
Mutagenesisi89 – 891K → S: No effect on PDCD1 binding. 1 Publication
Mutagenesisi98 – 981A → F: No effect on PDCD1 binding. 1 Publication
Mutagenesisi100 – 1001Q → S: No effect on PDCD1 binding.
Mutagenesisi113 – 1131C → Y: No effect on PDCD1 binding. 1 Publication
Mutagenesisi115 – 1151I → A: Abolishes the binding to PDCD1. 1 Publication
Mutagenesisi117 – 1171S → Y: No effect on PDCD1 binding. 1 Publication
Mutagenesisi124 – 1241K → A: Abolishes the binding to PDCD1. 1 Publication
Mutagenesisi126 – 1261I → A: Abolishes the binding to PDCD1. Costimulates proliferation and IFNG production of T-cells. 1 Publication
Mutagenesisi129 – 1291K → S: Abolishes the binding to PDCD1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 Reviewed predictionAdd
BLAST
Chaini19 – 290272Programmed cell death 1 ligand 1PRO_0000014554Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi40 ↔ 114 By similarity
Disulfide bondi154 ↔ 208 By similarity
Glycosylationi191 – 1911N-linked (GlcNAc...) Reviewed prediction
Glycosylationi199 – 1991N-linked (GlcNAc...) Reviewed prediction
Glycosylationi218 – 2181N-linked (GlcNAc...) Reviewed prediction
Glycosylationi236 – 2361N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ9EP73.

PTM databases

PhosphoSiteiQ9EP73.

Expressioni

Tissue specificityi

Highly expressed in the heart, thymus, skeletal muscle, and lung. Weakly expressed in the kidney, spleen, thyroid, and liver. Expressed on activated dendritic cells, B-cells and macrophages. Expressed in numerous tumor cells lines of lymphoid origin.3 Publications

Inductioni

Up-regulated by IFNG treatment in monocytes. Up-regulated on dendritic cells, B-cells and macrophages after activation by LPS and INFG.2 Publications

Gene expression databases

ArrayExpressiQ9EP73.
BgeeiQ9EP73.
CleanExiMM_CD274.
GenevestigatoriQ9EP73.

Interactioni

Subunit structurei

Interacts with PDCD1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Cd80Q006094EBI-5258879,EBI-5258929

Protein-protein interaction databases

DIPiDIP-46167N.
IntActiQ9EP73. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9EP73.
SMRiQ9EP73. Positions 19-229.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 127109Ig-like V-typeAdd
BLAST
Domaini133 – 22492Ig-like C2-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG43849.
GeneTreeiENSGT00650000093373.
HOGENOMiHOG000059625.
HOVERGENiHBG082112.
InParanoidiQ9EP73.
KOiK06745.
OMAiAEVIWTS.
OrthoDBiEOG7T7GTP.
PhylomeDBiQ9EP73.
TreeFamiTF331083.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9EP73-1 [UniParc]FASTAAdd to Basket

« Hide

MRIFAGIIFT ACCHLLRAFT ITAPKDLYVV EYGSNVTMEC RFPVERELDL    50
LALVVYWEKE DEQVIQFVAG EEDLKPQHSN FRGRASLPKD QLLKGNAALQ 100
ITDVKLQDAG VYCCIISYGG ADYKRITLKV NAPYRKINQR ISVDPATSEH 150
ELICQAEGYP EAEVIWTNSD HQPVSGKRSV TTSRTEGMLL NVTSSLRVNA 200
TANDVFYCTF WRSQPGQNHT AELIIPELPA THPPQNRTHW VLLGSILLFL 250
IVVSTVLLFL RKQVRMLDVE KCGVEDTSSK NRNDTQFEET 290
Length:290
Mass (Da):32,780
Last modified:March 1, 2001 - v1
Checksum:iAB7C46CF853EBB02
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF233517 mRNA. Translation: AAG18509.1.
AF317088 mRNA. Translation: AAG31810.1.
BC066841 mRNA. Translation: AAH66841.1.
CCDSiCCDS29735.1.
RefSeqiNP_068693.1. NM_021893.3.
UniGeneiMm.245363.

Genome annotation databases

EnsembliENSMUST00000016640; ENSMUSP00000016640; ENSMUSG00000016496.
GeneIDi60533.
KEGGimmu:60533.
UCSCiuc008hdi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF233517 mRNA. Translation: AAG18509.1 .
AF317088 mRNA. Translation: AAG31810.1 .
BC066841 mRNA. Translation: AAH66841.1 .
CCDSi CCDS29735.1.
RefSeqi NP_068693.1. NM_021893.3.
UniGenei Mm.245363.

3D structure databases

ProteinModelPortali Q9EP73.
SMRi Q9EP73. Positions 19-229.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-46167N.
IntActi Q9EP73. 2 interactions.

PTM databases

PhosphoSitei Q9EP73.

Proteomic databases

PRIDEi Q9EP73.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000016640 ; ENSMUSP00000016640 ; ENSMUSG00000016496 .
GeneIDi 60533.
KEGGi mmu:60533.
UCSCi uc008hdi.2. mouse.

Organism-specific databases

CTDi 29126.
MGIi MGI:1926446. Cd274.

Phylogenomic databases

eggNOGi NOG43849.
GeneTreei ENSGT00650000093373.
HOGENOMi HOG000059625.
HOVERGENi HBG082112.
InParanoidi Q9EP73.
KOi K06745.
OMAi AEVIWTS.
OrthoDBi EOG7T7GTP.
PhylomeDBi Q9EP73.
TreeFami TF331083.

Miscellaneous databases

ChiTaRSi CD274. mouse.
NextBioi 314969.
PROi Q9EP73.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9EP73.
Bgeei Q9EP73.
CleanExi MM_CD274.
Genevestigatori Q9EP73.

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
InterProi IPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view ]
Pfami PF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view ]
SMARTi SM00409. IG. 1 hit.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Engagement of the PD-1 immunoinhibitory receptor by a novel B7-family member leads to negative regulation of lymphocyte activation."
    Freeman G.J., Long A.J., Iwai Y., Bourque K., Chernova T., Nishimura H., Fitz L.J., Malenkovich N., Okazaki T., Byrne M.C., Horton H.F., Fouser L., Carter L., Ling V., Bowman M.R., Carreno B.M., Collins M., Wood C.R., Honjo T.
    J. Exp. Med. 192:1027-1034(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH PDCD1, TISSUE SPECIFICITY, INDUCTION.
    Tissue: Spleen.
  2. "B7-H1 costimulation preferentially enhances CD28-independent T-helper cell function."
    Tamura H., Dong H., Zhu G., Sica G.L., Flies D.B., Tamada K., Chen L.
    Blood 97:1809-1816(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    Strain: C57BL/6.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CD-1.
    Tissue: Neural stem cell.
  4. Cited for: TISSUE SPECIFICITY.
  5. "Molecular modeling and functional mapping of B7-H1 and B7-DC uncouple costimulatory function from PD-1 interaction."
    Wang S., Bajorath J., Flies D.B., Dong H., Honjo T., Chen L.
    J. Exp. Med. 197:1083-1091(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF LEU-27; GLU-31; SER-34; THR-37; ASP-49; TYR-56; GLU-58; GLU-62; PHE-67; ALA-69; GLU-72; LYS-75; LYS-89; ALA-98; CYS-113; ILE-115; SER-117; LYS-124; ILE-126 AND LYS-129.

Entry informationi

Entry nameiPD1L1_MOUSE
AccessioniPrimary (citable) accession number: Q9EP73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi