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Protein

Programmed cell death 1 ligand 1

Gene

Cd274

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the costimulatory signal essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.3 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Enzyme and pathway databases

ReactomeiR-MMU-389948. PD-1 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Programmed cell death 1 ligand 1
Short name:
PD-L1
Short name:
PDCD1 ligand 1
Short name:
Programmed death ligand 1
Alternative name(s):
B7 homolog 1
Short name:
B7-H1
CD_antigen: CD274
Gene namesi
Name:Cd274
Synonyms:B7h1, Pdcd1l1, Pdcd1lg1, Pdl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1926446. Cd274.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 239ExtracellularSequence analysisAdd BLAST221
Transmembranei240 – 260HelicalSequence analysisAdd BLAST21
Topological domaini261 – 290CytoplasmicSequence analysisAdd BLAST30

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27L → A: PDCD1 binding. 1 Publication1
Mutagenesisi31E → S: Significantly reduces the binding to PDCD1. 1 Publication1
Mutagenesisi34S → Y: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi37T → Y: Significantly reduces the binding to PDCD1. 1 Publication1
Mutagenesisi49D → S: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi56Y → S: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi58E → S: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi62E → S: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi67F → A: Abolishes the binding to PDCD1. Costimulates proliferation and IFNG production of T-cells. 1 Publication1
Mutagenesisi69A → F: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi72E → S: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi75K → S: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi89K → S: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi98A → F: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi100Q → S: No effect on PDCD1 binding. 1
Mutagenesisi113C → Y: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi115I → A: Abolishes the binding to PDCD1. 1 Publication1
Mutagenesisi117S → Y: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi124K → A: Abolishes the binding to PDCD1. 1 Publication1
Mutagenesisi126I → A: Abolishes the binding to PDCD1. Costimulates proliferation and IFNG production of T-cells. 1 Publication1
Mutagenesisi129K → S: Abolishes the binding to PDCD1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000001455419 – 290Programmed cell death 1 ligand 1Add BLAST272

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi35N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi40 ↔ 114PROSITE-ProRule annotation
Disulfide bondi154 ↔ 208PROSITE-ProRule annotation
Glycosylationi191N-linked (GlcNAc...)Sequence analysis1
Glycosylationi199N-linked (GlcNAc...)Sequence analysis1
Glycosylationi218N-linked (GlcNAc...)Sequence analysis1
Glycosylationi236N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9EP73.
PaxDbiQ9EP73.
PRIDEiQ9EP73.

PTM databases

PhosphoSitePlusiQ9EP73.

Expressioni

Tissue specificityi

Highly expressed in the heart, thymus, skeletal muscle, and lung. Weakly expressed in the kidney, spleen, thyroid, and liver. Expressed on activated dendritic cells, B-cells and macrophages. Expressed in numerous tumor cells lines of lymphoid origin.3 Publications

Inductioni

Up-regulated by IFNG treatment in monocytes. Up-regulated on dendritic cells, B-cells and macrophages after activation by LPS and IFNG.2 Publications

Gene expression databases

BgeeiENSMUSG00000016496.
CleanExiMM_CD274.
ExpressionAtlasiQ9EP73. baseline and differential.
GenevisibleiQ9EP73. MM.

Interactioni

Subunit structurei

Interacts with PDCD1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Cd80Q006094EBI-5258879,EBI-5258929

Protein-protein interaction databases

DIPiDIP-46167N.
IntActiQ9EP73. 2 interactors.
STRINGi10090.ENSMUSP00000016640.

Structurei

3D structure databases

ProteinModelPortaliQ9EP73.
SMRiQ9EP73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 127Ig-like V-typeAdd BLAST109
Domaini133 – 224Ig-like C2-typeAdd BLAST92

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIBU. Eukaryota.
ENOG4111V14. LUCA.
GeneTreeiENSGT00650000093373.
HOGENOMiHOG000059625.
HOVERGENiHBG082112.
InParanoidiQ9EP73.
KOiK06745.
OMAiAEVIWTS.
OrthoDBiEOG091G0F5W.
PhylomeDBiQ9EP73.
TreeFamiTF331083.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9EP73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIFAGIIFT ACCHLLRAFT ITAPKDLYVV EYGSNVTMEC RFPVERELDL
60 70 80 90 100
LALVVYWEKE DEQVIQFVAG EEDLKPQHSN FRGRASLPKD QLLKGNAALQ
110 120 130 140 150
ITDVKLQDAG VYCCIISYGG ADYKRITLKV NAPYRKINQR ISVDPATSEH
160 170 180 190 200
ELICQAEGYP EAEVIWTNSD HQPVSGKRSV TTSRTEGMLL NVTSSLRVNA
210 220 230 240 250
TANDVFYCTF WRSQPGQNHT AELIIPELPA THPPQNRTHW VLLGSILLFL
260 270 280 290
IVVSTVLLFL RKQVRMLDVE KCGVEDTSSK NRNDTQFEET
Length:290
Mass (Da):32,780
Last modified:March 1, 2001 - v1
Checksum:iAB7C46CF853EBB02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233517 mRNA. Translation: AAG18509.1.
AF317088 mRNA. Translation: AAG31810.1.
BC066841 mRNA. Translation: AAH66841.1.
CCDSiCCDS29735.1.
RefSeqiNP_068693.1. NM_021893.3.
UniGeneiMm.245363.

Genome annotation databases

EnsembliENSMUST00000016640; ENSMUSP00000016640; ENSMUSG00000016496.
GeneIDi60533.
KEGGimmu:60533.
UCSCiuc008hdi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233517 mRNA. Translation: AAG18509.1.
AF317088 mRNA. Translation: AAG31810.1.
BC066841 mRNA. Translation: AAH66841.1.
CCDSiCCDS29735.1.
RefSeqiNP_068693.1. NM_021893.3.
UniGeneiMm.245363.

3D structure databases

ProteinModelPortaliQ9EP73.
SMRiQ9EP73.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46167N.
IntActiQ9EP73. 2 interactors.
STRINGi10090.ENSMUSP00000016640.

PTM databases

PhosphoSitePlusiQ9EP73.

Proteomic databases

MaxQBiQ9EP73.
PaxDbiQ9EP73.
PRIDEiQ9EP73.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000016640; ENSMUSP00000016640; ENSMUSG00000016496.
GeneIDi60533.
KEGGimmu:60533.
UCSCiuc008hdi.2. mouse.

Organism-specific databases

CTDi29126.
MGIiMGI:1926446. Cd274.

Phylogenomic databases

eggNOGiENOG410IIBU. Eukaryota.
ENOG4111V14. LUCA.
GeneTreeiENSGT00650000093373.
HOGENOMiHOG000059625.
HOVERGENiHBG082112.
InParanoidiQ9EP73.
KOiK06745.
OMAiAEVIWTS.
OrthoDBiEOG091G0F5W.
PhylomeDBiQ9EP73.
TreeFamiTF331083.

Enzyme and pathway databases

ReactomeiR-MMU-389948. PD-1 signaling.

Miscellaneous databases

ChiTaRSiCd274. mouse.
PROiQ9EP73.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000016496.
CleanExiMM_CD274.
ExpressionAtlasiQ9EP73. baseline and differential.
GenevisibleiQ9EP73. MM.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPD1L1_MOUSE
AccessioniPrimary (citable) accession number: Q9EP73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.