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Q9EP73

- PD1L1_MOUSE

UniProt

Q9EP73 - PD1L1_MOUSE

Protein

Programmed cell death 1 ligand 1

Gene

Cd274

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Involved in the costimulatory signal essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. cell surface receptor signaling pathway Source: Ensembl
    2. immune response Source: Ensembl
    3. negative regulation of T cell proliferation Source: MGI
    4. positive regulation of interleukin-10 secretion Source: Ensembl
    5. T cell costimulation Source: Ensembl

    Keywords - Molecular functioni

    Receptor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Programmed cell death 1 ligand 1
    Short name:
    PD-L1
    Short name:
    PDCD1 ligand 1
    Short name:
    Programmed death ligand 1
    Alternative name(s):
    B7 homolog 1
    Short name:
    B7-H1
    CD_antigen: CD274
    Gene namesi
    Name:Cd274
    Synonyms:B7h1, Pdcd1l1, Pdcd1lg1, Pdl1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:1926446. Cd274.

    Subcellular locationi

    GO - Cellular componenti

    1. external side of plasma membrane Source: MGI
    2. extracellular vesicular exosome Source: Ensembl
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi27 – 271L → A: PDCD1 binding. 1 Publication
    Mutagenesisi31 – 311E → S: Significantly reduces the binding to PDCD1. 1 Publication
    Mutagenesisi34 – 341S → Y: No effect on PDCD1 binding. 1 Publication
    Mutagenesisi37 – 371T → Y: Significantly reduces the binding to PDCD1. 1 Publication
    Mutagenesisi49 – 491D → S: No effect on PDCD1 binding. 1 Publication
    Mutagenesisi56 – 561Y → S: No effect on PDCD1 binding. 1 Publication
    Mutagenesisi58 – 581E → S: No effect on PDCD1 binding. 1 Publication
    Mutagenesisi62 – 621E → S: No effect on PDCD1 binding. 1 Publication
    Mutagenesisi67 – 671F → A: Abolishes the binding to PDCD1. Costimulates proliferation and IFNG production of T-cells. 1 Publication
    Mutagenesisi69 – 691A → F: No effect on PDCD1 binding. 1 Publication
    Mutagenesisi72 – 721E → S: No effect on PDCD1 binding. 1 Publication
    Mutagenesisi75 – 751K → S: No effect on PDCD1 binding. 1 Publication
    Mutagenesisi89 – 891K → S: No effect on PDCD1 binding. 1 Publication
    Mutagenesisi98 – 981A → F: No effect on PDCD1 binding. 1 Publication
    Mutagenesisi100 – 1001Q → S: No effect on PDCD1 binding.
    Mutagenesisi113 – 1131C → Y: No effect on PDCD1 binding. 1 Publication
    Mutagenesisi115 – 1151I → A: Abolishes the binding to PDCD1. 1 Publication
    Mutagenesisi117 – 1171S → Y: No effect on PDCD1 binding. 1 Publication
    Mutagenesisi124 – 1241K → A: Abolishes the binding to PDCD1. 1 Publication
    Mutagenesisi126 – 1261I → A: Abolishes the binding to PDCD1. Costimulates proliferation and IFNG production of T-cells. 1 Publication
    Mutagenesisi129 – 1291K → S: Abolishes the binding to PDCD1. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 290272Programmed cell death 1 ligand 1PRO_0000014554Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi40 ↔ 114PROSITE-ProRule annotation
    Disulfide bondi154 ↔ 208PROSITE-ProRule annotation
    Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi199 – 1991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi218 – 2181N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ9EP73.

    PTM databases

    PhosphoSiteiQ9EP73.

    Expressioni

    Tissue specificityi

    Highly expressed in the heart, thymus, skeletal muscle, and lung. Weakly expressed in the kidney, spleen, thyroid, and liver. Expressed on activated dendritic cells, B-cells and macrophages. Expressed in numerous tumor cells lines of lymphoid origin.3 Publications

    Inductioni

    Up-regulated by IFNG treatment in monocytes. Up-regulated on dendritic cells, B-cells and macrophages after activation by LPS and INFG.2 Publications

    Gene expression databases

    ArrayExpressiQ9EP73.
    BgeeiQ9EP73.
    CleanExiMM_CD274.
    GenevestigatoriQ9EP73.

    Interactioni

    Subunit structurei

    Interacts with PDCD1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Cd80Q006094EBI-5258879,EBI-5258929

    Protein-protein interaction databases

    DIPiDIP-46167N.
    IntActiQ9EP73. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9EP73.
    SMRiQ9EP73. Positions 19-229.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini19 – 239221ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini261 – 29030CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei240 – 26021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini19 – 127109Ig-like V-typeAdd
    BLAST
    Domaini133 – 22492Ig-like C2-typeAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG43849.
    GeneTreeiENSGT00650000093373.
    HOGENOMiHOG000059625.
    HOVERGENiHBG082112.
    InParanoidiQ9EP73.
    KOiK06745.
    OMAiAEVIWTS.
    OrthoDBiEOG7T7GTP.
    PhylomeDBiQ9EP73.
    TreeFamiTF331083.

    Family and domain databases

    Gene3Di2.60.40.10. 2 hits.
    InterProiIPR013162. CD80_C2-set.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    [Graphical view]
    PfamiPF08205. C2-set_2. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 1 hit.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9EP73-1 [UniParc]FASTAAdd to Basket

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    MRIFAGIIFT ACCHLLRAFT ITAPKDLYVV EYGSNVTMEC RFPVERELDL    50
    LALVVYWEKE DEQVIQFVAG EEDLKPQHSN FRGRASLPKD QLLKGNAALQ 100
    ITDVKLQDAG VYCCIISYGG ADYKRITLKV NAPYRKINQR ISVDPATSEH 150
    ELICQAEGYP EAEVIWTNSD HQPVSGKRSV TTSRTEGMLL NVTSSLRVNA 200
    TANDVFYCTF WRSQPGQNHT AELIIPELPA THPPQNRTHW VLLGSILLFL 250
    IVVSTVLLFL RKQVRMLDVE KCGVEDTSSK NRNDTQFEET 290
    Length:290
    Mass (Da):32,780
    Last modified:March 1, 2001 - v1
    Checksum:iAB7C46CF853EBB02
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF233517 mRNA. Translation: AAG18509.1.
    AF317088 mRNA. Translation: AAG31810.1.
    BC066841 mRNA. Translation: AAH66841.1.
    CCDSiCCDS29735.1.
    RefSeqiNP_068693.1. NM_021893.3.
    UniGeneiMm.245363.

    Genome annotation databases

    EnsembliENSMUST00000016640; ENSMUSP00000016640; ENSMUSG00000016496.
    GeneIDi60533.
    KEGGimmu:60533.
    UCSCiuc008hdi.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF233517 mRNA. Translation: AAG18509.1 .
    AF317088 mRNA. Translation: AAG31810.1 .
    BC066841 mRNA. Translation: AAH66841.1 .
    CCDSi CCDS29735.1.
    RefSeqi NP_068693.1. NM_021893.3.
    UniGenei Mm.245363.

    3D structure databases

    ProteinModelPortali Q9EP73.
    SMRi Q9EP73. Positions 19-229.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-46167N.
    IntActi Q9EP73. 2 interactions.

    PTM databases

    PhosphoSitei Q9EP73.

    Proteomic databases

    PRIDEi Q9EP73.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000016640 ; ENSMUSP00000016640 ; ENSMUSG00000016496 .
    GeneIDi 60533.
    KEGGi mmu:60533.
    UCSCi uc008hdi.2. mouse.

    Organism-specific databases

    CTDi 29126.
    MGIi MGI:1926446. Cd274.

    Phylogenomic databases

    eggNOGi NOG43849.
    GeneTreei ENSGT00650000093373.
    HOGENOMi HOG000059625.
    HOVERGENi HBG082112.
    InParanoidi Q9EP73.
    KOi K06745.
    OMAi AEVIWTS.
    OrthoDBi EOG7T7GTP.
    PhylomeDBi Q9EP73.
    TreeFami TF331083.

    Miscellaneous databases

    ChiTaRSi CD274. mouse.
    NextBioi 314969.
    PROi Q9EP73.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9EP73.
    Bgeei Q9EP73.
    CleanExi MM_CD274.
    Genevestigatori Q9EP73.

    Family and domain databases

    Gene3Di 2.60.40.10. 2 hits.
    InterProi IPR013162. CD80_C2-set.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    [Graphical view ]
    Pfami PF08205. C2-set_2. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 1 hit.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Engagement of the PD-1 immunoinhibitory receptor by a novel B7-family member leads to negative regulation of lymphocyte activation."
      Freeman G.J., Long A.J., Iwai Y., Bourque K., Chernova T., Nishimura H., Fitz L.J., Malenkovich N., Okazaki T., Byrne M.C., Horton H.F., Fouser L., Carter L., Ling V., Bowman M.R., Carreno B.M., Collins M., Wood C.R., Honjo T.
      J. Exp. Med. 192:1027-1034(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH PDCD1, TISSUE SPECIFICITY, INDUCTION.
      Tissue: Spleen.
    2. "B7-H1 costimulation preferentially enhances CD28-independent T-helper cell function."
      Tamura H., Dong H., Zhu G., Sica G.L., Flies D.B., Tamada K., Chen L.
      Blood 97:1809-1816(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
      Strain: C57BL/6.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: CD-1.
      Tissue: Neural stem cell.
    4. Cited for: TISSUE SPECIFICITY.
    5. "Molecular modeling and functional mapping of B7-H1 and B7-DC uncouple costimulatory function from PD-1 interaction."
      Wang S., Bajorath J., Flies D.B., Dong H., Honjo T., Chen L.
      J. Exp. Med. 197:1083-1091(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF LEU-27; GLU-31; SER-34; THR-37; ASP-49; TYR-56; GLU-58; GLU-62; PHE-67; ALA-69; GLU-72; LYS-75; LYS-89; ALA-98; CYS-113; ILE-115; SER-117; LYS-124; ILE-126 AND LYS-129.

    Entry informationi

    Entry nameiPD1L1_MOUSE
    AccessioniPrimary (citable) accession number: Q9EP73
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2005
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3