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Q9EP52

- TWSG1_MOUSE

UniProt

Q9EP52 - TWSG1_MOUSE

Protein

Twisted gastrulation protein homolog 1

Gene

Twsg1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    May be involved in dorsoventral axis formation. Seems to antagonize BMP signaling by forming ternary complexes with CHRD and BMPs, thereby preventing BMPs from binding to their receptors. In addition to the anti-BMP function, also has pro-BMP activity, partly mediated by cleavage and degradation of CHRD, which releases BMPs from ternary complexes. May be an important modulator of BMP-regulated cartilage development and chondrocyte differentiation. May play a role in thymocyte development.4 Publications

    GO - Molecular functioni

    1. protein binding Source: MGI

    GO - Biological processi

    1. BMP signaling pathway Source: MGI
    2. camera-type eye development Source: MGI
    3. cell differentiation Source: MGI
    4. forebrain development Source: MGI
    5. hemopoiesis Source: MGI
    6. mesoderm formation Source: MGI
    7. negative regulation of BMP signaling pathway Source: MGI
    8. negative regulation of osteoblast differentiation Source: MGI
    9. ossification Source: MGI
    10. positive regulation of BMP signaling pathway Source: MGI
    11. salivary gland morphogenesis Source: MGI
    12. tissue development Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Twisted gastrulation protein homolog 1
    Gene namesi
    Name:Twsg1
    Synonyms:Tsg
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:2137520. Twsg1.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular space Source: MGI

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Embryonic lethality and sirenomelia were observed only in BMP null embryos in which one or two copies of TSG were also missing. When TSG and BMP7 are mutated, the siren phenotype results from the fusion of the limb buds in the ventroposterior midline owing to a paucity of posterior ventral mesoderm.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 222198Twisted gastrulation protein homolog 1PRO_0000278809Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi146 – 1461N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9EP52.
    PRIDEiQ9EP52.

    Expressioni

    Tissue specificityi

    Expressed in lymph node, liver, kidney, and lung. Expression in the kidney was stronger in the medulla than in the cortex, particularly in the cells surrounding the medullary tubules. Expressed in growth plate cartilage of long bones, ribs, and digits and to a lesser extent also in the resting zone of the epiphysis, trabecular bone, and vertebral cartilage. Expression seems to be absent from other skeletal tissues including muscle, skin, and fibroblasts.1 Publication

    Developmental stagei

    Expressed at all embryonic stages examined. Expression was low and distribution was diffuse. At the primitive streak stage, detected in the extraembryonic region. Signal first appeared in the embryo during the neural plate stage. Stronger and more localized expression is seen after embryonic turning. Higher expression is seen in the branchial arch mesenchyme, the endoderm of the developing pharynx, all levels of the developing gut, the myotome compartment of the somites, and some regions of surface ectoderm. At 8.25 and 9.0 dpc expressed in head mesenchyme and ventral mesoderm.2 Publications

    Gene expression databases

    BgeeiQ9EP52.
    CleanExiMM_TWSG1.
    GenevestigatoriQ9EP52.

    Interactioni

    Subunit structurei

    Interacts with CHRD and/or BMP4. This interaction enhances CHRD/BMP4 complex formation. Interacts with BMP7.3 Publications

    Protein-protein interaction databases

    BioGridi211157. 1 interaction.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi18 – 7659Cys-richAdd
    BLAST

    Domaini

    The N-terminal domain is sufficient to interact with BMP4.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG70213.
    GeneTreeiENSGT00390000007058.
    HOGENOMiHOG000044674.
    HOVERGENiHBG055753.
    InParanoidiQ9EP52.
    OMAiNCTVAFM.
    OrthoDBiEOG7J70GN.
    PhylomeDBiQ9EP52.
    TreeFamiTF323922.

    Family and domain databases

    InterProiIPR006761. Tsg.
    [Graphical view]
    PANTHERiPTHR12312. PTHR12312. 1 hit.
    PfamiPF04668. Tsg. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9EP52-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSHYIVLAL ASLTFLLCLP VSQSCNKALC ASDVSKCLIQ ELCQCRPGEG    50
    NCPCCKECML CLGALWDECC DCVGMCNPRN YSDTPPTSKS TVEELHEPIP 100
    SLFRALTEGD TQLNWNIVSF PVAEELSHHE NLVSFLETVN QLHHQNVSVP 150
    SNNVHAPFPS DKERMCTVVY FDDCMSIHQC KISCESMGAS KYRWFHNACC 200
    ECIGPECIDY GSKTVKCMNC MF 222
    Length:222
    Mass (Da):24,801
    Last modified:March 1, 2001 - v1
    Checksum:iF9B0CEE2A8DF8CA7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 61I → IA in AAG10080. 1 PublicationCurated
    Sequence conflicti75 – 751M → R in AAG10080. 1 PublicationCurated
    Sequence conflicti75 – 751M → R in AAH04850. (PubMed:15489334)Curated
    Sequence conflicti168 – 1681V → E in BAC28326. (PubMed:16141072)Curated
    Sequence conflicti203 – 2042IG → MR in AAG10080. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ297390 mRNA. Translation: CAC05586.1.
    AF292033 mRNA. Translation: AAG00605.1.
    AF295097 mRNA. Translation: AAG10080.1.
    AK018524 mRNA. Translation: BAC25557.1.
    AK033504 mRNA. Translation: BAC28326.1.
    AK049646 mRNA. Translation: BAC33857.1.
    AK168547 mRNA. Translation: BAE40422.1.
    AK168633 mRNA. Translation: BAE40493.1.
    BC004850 mRNA. Translation: AAH04850.1.
    CCDSiCCDS28942.1.
    RefSeqiNP_075540.1. NM_023053.3.
    UniGeneiMm.10153.

    Genome annotation databases

    EnsembliENSMUST00000024906; ENSMUSP00000024906; ENSMUSG00000024098.
    GeneIDi65960.
    KEGGimmu:65960.
    UCSCiuc008dgq.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ297390 mRNA. Translation: CAC05586.1 .
    AF292033 mRNA. Translation: AAG00605.1 .
    AF295097 mRNA. Translation: AAG10080.1 .
    AK018524 mRNA. Translation: BAC25557.1 .
    AK033504 mRNA. Translation: BAC28326.1 .
    AK049646 mRNA. Translation: BAC33857.1 .
    AK168547 mRNA. Translation: BAE40422.1 .
    AK168633 mRNA. Translation: BAE40493.1 .
    BC004850 mRNA. Translation: AAH04850.1 .
    CCDSi CCDS28942.1.
    RefSeqi NP_075540.1. NM_023053.3.
    UniGenei Mm.10153.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 211157. 1 interaction.

    Proteomic databases

    PaxDbi Q9EP52.
    PRIDEi Q9EP52.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000024906 ; ENSMUSP00000024906 ; ENSMUSG00000024098 .
    GeneIDi 65960.
    KEGGi mmu:65960.
    UCSCi uc008dgq.2. mouse.

    Organism-specific databases

    CTDi 57045.
    MGIi MGI:2137520. Twsg1.

    Phylogenomic databases

    eggNOGi NOG70213.
    GeneTreei ENSGT00390000007058.
    HOGENOMi HOG000044674.
    HOVERGENi HBG055753.
    InParanoidi Q9EP52.
    OMAi NCTVAFM.
    OrthoDBi EOG7J70GN.
    PhylomeDBi Q9EP52.
    TreeFami TF323922.

    Miscellaneous databases

    NextBioi 320380.
    PROi Q9EP52.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9EP52.
    CleanExi MM_TWSG1.
    Genevestigatori Q9EP52.

    Family and domain databases

    InterProi IPR006761. Tsg.
    [Graphical view ]
    PANTHERi PTHR12312. PTHR12312. 1 hit.
    Pfami PF04668. Tsg. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolutionary conservation, developmental expression, and genomic mapping of mammalian Twisted gastrulation."
      Graf D., Timmons P.M., Hitchins M., Episkopou V., Moore G., Ito T., Fujiyama A., Fisher A.G., Merkenschlager M.
      Mamm. Genome 12:554-560(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CHRD AND BMP4.
      Tissue: Thymus.
    2. "Characterization of the mouse homolog of Twisted gastrulation."
      Zakin L., Oelgeschlager M., Geissert D., De Robertis E.M.
      Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Mouse twisted gastrulation protein: a new signal pathway of spermatogenesis."
      Li J.M., Sha J.H., Zhou Z.M.
      Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Amnion, Colon, Liver and Spinal cord.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.
    6. "Homologues of Twisted gastrulation are extracellular cofactors in antagonism of BMP signalling."
      Scott I.C., Blitz I.L., Pappano W.N., Maas S.A., Cho K.W.Y., Greenspan D.S.
      Nature 410:475-478(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE, INTERACTION WITH CHRD AND BMP4.
    7. "The developmentally regulated expression of Twisted gastrulation reveals a role for bone morphogenetic proteins in the control of T cell development."
      Graf D., Nethisinghe S., Palmer D.B., Fisher A.G., Merkenschlager M.
      J. Exp. Med. 196:163-171(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Sirenomelia in Bmp7 and Tsg compound mutant mice: requirement for Bmp signaling in the development of ventral posterior mesoderm."
      Zakin L., Reversade B., Kuroda H., Lyons K.M., De Robertis E.M.
      Development 132:2489-2499(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, INTERACTION WITH BMP7.
    9. "Twisted gastrulation modulates bone morphogenetic protein-induced collagen II and X expression in chondrocytes in vitro and in vivo."
      Schmidl M., Adam N., Surmann-Schmitt C., Hattori T., Stock M., Dietz U., de Crombrugghe B., Poeschl E., von der Mark K.
      J. Biol. Chem. 281:31790-31800(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiTWSG1_MOUSE
    AccessioniPrimary (citable) accession number: Q9EP52
    Secondary accession number(s): Q8CCB1
    , Q8CEM6, Q99K77, Q9ERN7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 6, 2007
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3