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Q9E8F2

- NSP5_ROTRF

UniProt

Q9E8F2 - NSP5_ROTRF

Protein

Non-structural protein 5

Gene
N/A
Organism
Rotavirus A (strain Cow/France/RF/1975 G6-P6[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3) (RV-A)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 29 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Involved in genome replication. Plays a crucial role, together with NSP2, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where core-like replication intermediates are assembled and RNA replication takes place. May regulate NSP2-RNA interactions during genome replication, since NSP5 competes with RNA for the same binding site on the NSP2 octamer. Binds to either ssRNA or dsRNA with similar affinities. Displays ATPase and autokinase activities.

    Cofactori

    Magnesium for ATPase activity.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi92 – 921MagnesiumSequence Analysis

    GO - Molecular functioni

    1. ATPase activity Source: InterPro
    2. magnesium ion binding Source: InterPro
    3. nucleotide binding Source: UniProtKB-KW
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. viral genome replication Source: InterPro

    Keywords - Ligandi

    Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-structural protein 5
    Short name:
    NSP5
    Alternative name(s):
    NS26
    OrganismiRotavirus A (strain Cow/France/RF/1975 G6-P6[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3) (RV-A)
    Taxonomic identifieri10933 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    ProteomesiUP000007179: Genome

    Subcellular locationi

    Host cytoplasm 1 Publication
    Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 198198Non-structural protein 5PRO_0000367824Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei67 – 671Phosphoserine; by host CK1By similarity
    Modified residuei154 – 1541Phosphoserine; by hostBy similarity
    Modified residuei156 – 1561Phosphoserine; by hostBy similarity
    Modified residuei164 – 1641Phosphoserine; by hostBy similarity
    Modified residuei166 – 1661Phosphoserine; by hostBy similarity

    Post-translational modificationi

    O-glycosylated.By similarity
    Hyperphosphorylated on serine residues, when in dimeric form. Ser-67 phosphorylation by CK1 is required for the hyperphosphorylation of NSP5 dimer. Impaired phosphorylation is associated with a profound morphological change in virus factories and a moderate decrease in virus replication.

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Interactioni

    Subunit structurei

    Homodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories. Interacts with NSP6 By similarity.By similarity

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 4848Interaction with VP1By similarityAdd
    BLAST
    Regioni189 – 19810Homodimerization and interaction with NSP6By similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi2 – 101100Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the rotavirus A NSP5 family.Curated

    Family and domain databases

    InterProiIPR002512. Rotavirus_A/C_NSP5.
    [Graphical view]
    PfamiPF01525. Rota_NS26. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004006. Rota_NS26. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9E8F2-1 [UniParc]FASTAAdd to Basket

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    MSLSIDVTSL PSISSSIYKN ESSSTTSTLS GKSIGRSEQY ISPDAEAFSK    50
    YMLSKSPEDI GPSDSASNDP LTSFSIRSNA VKTNADAGVS MDSSTQSRPS 100
    SNVGCDQVDF SFNKGISMNA NLDSSISIST SSKKEKSKSD HKSRKHYPKI 150
    EAESDSDDYI LDDSDSDDGK CKNCKYKRKY FALRMRMKQV AMQLIEDL 198
    Length:198
    Mass (Da):21,644
    Last modified:March 1, 2001 - v1
    Checksum:iF8CE19C16E0EA4B6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF188126 mRNA. Translation: AAG15311.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF188126 mRNA. Translation: AAG15311.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002512. Rotavirus_A/C_NSP5.
    [Graphical view ]
    Pfami PF01525. Rota_NS26. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004006. Rota_NS26. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Duarte M., Castagne N., Poncet D.
      Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "In vivo and in vitro phosphorylation of rotavirus NSP5 correlates with its localization in viroplasms."
      Poncet D., Lindenbaum P., L'Haridon R., Cohen J.
      J. Virol. 71:34-41(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, SUBCELLULAR LOCATION.
    3. "Rotavirus nonstructural protein NSP5 interacts with major core protein VP2."
      Berois M., Sapin C., Erk I., Poncet D., Cohen J.
      J. Virol. 77:1757-1763(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH VP2.

    Entry informationi

    Entry nameiNSP5_ROTRF
    AccessioniPrimary (citable) accession number: Q9E8F2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 29 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3