Q9E784 (L_BEFVB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 3 domains:

1. RNA-directed RNA polymerase
   EC=2.7.7.48
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56
3. mRNA guanylyltransferase
   EC=2.7.7.-

Gene names

Name:

L

Organism

Bovine ephemeral fever virus (strain BB7721) (BEFV)

Taxonomic identifier

928297 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Dimarhabdovirus supergroup › Ephemerovirus

Virus host

Bos taurus (Bovine) [TaxID: 9913]
Syncerus caffer (Cape buffalo) [TaxID: 9970]
Culicoides [TaxID: 58271]
Bubalus bubalis (Domestic water buffalo) [TaxID: 89462]

Protein attributes

Sequence length	2144 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the rhabdoviridae protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2144	2144	RNA-directed RNA polymerase L		PRO_0000297833
Regions
	Domain	624 – 810	187	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q9E784 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 4C6C3E6701D340BC	FASTA	2,144	249,787
	10 20 30 40 50 60 MDSVDFLDSE DHDQNSWNGE EFGDDFLEYI WDQDEEESMD LINNKDYNLN SPLIIDPLVE 70 80 90 100 110 120 LRNWINNEKG HSESDLRSQQ SFEFKEFDII KRVLRNFINN VNLRRPEDSH HIFAKFLNQA 130 140 150 160 170 180 IVTNDYWNLG ETLSKIVEDI REVGASFYRG LDLDENIIID NIRRNWNDVP QLGKSWFLKF 190 200 210 220 230 240 FELHRVICVM NARSNIELKN LQSKNKLKKI KLPKELEERG HKCWIFDLDI SGRWIIFDNY 250 260 270 280 290 300 AYWELQRIVL NREFILMMKD VLISRFQTVL SMNVCTDEYK YTEENIETMM SLYREGDLIL 310 320 330 340 350 360 EEHGNKSYKG LKLLESICNL RLIKIVRKSR PKIPEFPNFE NHIYSSLNDL RVERGIDLSK 370 380 390 400 410 420 FSNIILREES IDMVLAFYSS FRHFGHPWID YLTGLDKLES QVNKDCQVDI QYANLLASDL 430 440 450 460 470 480 AFKILRKNFL EKKCWSVDKN KMDKKHKLYH HISHNTWPTQ QIIDEFGDHW HELPIIQCYE 490 500 510 520 530 540 IPDMIDISQI YSDKSHSLDR SEVLKIIKEQ KHKRIPTKRV LQTLLEKPAT NWPEFLKAVN 550 560 570 580 590 600 DYGLDWEKLV IGLKAKEREL KEEGRFFSLM SYELRDYFVS TEYLIKKYFV PLFEGLTMAD 610 620 630 640 650 660 DLNTVIKKML DVSSGQGTRE YEYITIANNI DYEKWNNYQR IESNGPVFTV MGRFLGLPNL 670 680 690 700 710 720 FTRTHEFFQK SLIYYNQRPD LMMVRGRECL NRLGVKVCWE GQKGGLEGLR QKGWSILNYL 730 740 750 760 770 780 MIERESRVRN TRVKILAQGD NQTISMCYKT ESWQNEEELD NHIKNMVSNN NQIMQAIING 790 800 810 820 830 840 TEKLGLRINL DETMTSADYI NYGKVPIIEG TIKGLPTKRW SRVNFTSNDQ LPSTSTVINS 850 860 870 880 890 900 SSTNALTVGH FSERPHDAIN GHLLFGSLGL LLLDYHNPAI RGQISEFIPE ANINNKLYNI 910 920 930 940 950 960 LLLYLDPSLG GIAGTSLTRF FIRGFPDGVT ESLTFWKIVG EYTNDQDIKR LASTVGSPEL 970 980 990 1000 1010 1020 SPFKPEDLDK LIEKPESLNI KHGLSSSNMI KGEVKKNIIE NCSKIQNEII RDAARNLVSE 1030 1040 1050 1060 1070 1080 ENQLFLWLRT INPLFPRFLS QFAESTYYGV TKSLINLFTN SKTIRGIYKK KYRKELDQLM 1090 1100 1110 1120 1130 1140 IKGEVRSIFG LIKIVNRSKQ FVMPIWDCSA SLADSLRKRS WGKEVLGTTV PHPAEMFKGY 1150 1160 1170 1180 1190 1200 RGGEDSCSFC RGNGSNNNYL TVLMPRGIPM KCHYRGPYYP YLGSNTKEST SILQPWEKET 1210 1220 1230 1240 1250 1260 KVPVLKRACD LRKSINWFVT PDSLLAKSIF NNLKALTGED WEDQIKGYKR TGSSLHRFGC 1270 1280 1290 1300 1310 1320 SRVSSGGFSA SSPSCFTWCI ATTDTMCGLG EVNYDFMFQS TLVWCQMSSI IRERGNLHSK 1330 1340 1350 1360 1370 1380 IHHYHIKCNK CLREIQEPVL ESGWEYQPRN VSQILEKWRP KNMKTWGEEK IHMDIKDNDD 1390 1400 1410 1420 1430 1440 EWDNLTVEDK SYEIGKTIGW LVGDSLLSHK RNYEFKSLFP VSIRYKLEGL PFLEGILDGF 1450 1460 1470 1480 1490 1500 KICGSLNLTH RRNYMILKKP KLALQGTVFF LIDRSSFISE FTNFISHPKI YKAIKSLPHK 1510 1520 1530 1540 1550 1560 IPTSYPMNLS DLGSILRSFL KQLYYRRKEL IIKKSSWVFS DMRTNEVICS FGLSHLTYRI 1570 1580 1590 1600 1610 1620 LIQEGLNKDM KLRLQQCQDL YINIMTESKE ELDKSSAKRE IVECLRELKF VSSEIRHAVK 1630 1640 1650 1660 1670 1680 FRYITGEKGE VQLIKEMEER RTWGDEYTGK ANLLDVWYLT SQSSENKNLV KGIKIPYHSN 1690 1700 1710 1720 1730 1740 PTISGLRINQ IATGAHYKLR TLINMTRITY RDFICGGDGS GGMTSCLLRL KPLSRGVFNS 1750 1760 1770 1780 1790 1800 LLILDDKPLH GTRPSPPTAI MELGEDSLRC VNCYDVWKEP SDLSKQETWK YFVKLKKQNS 1810 1820 1830 1840 1850 1860 MMIDLIVLDM EIINDEVIED IYQNTKNHLI YLLEEGGCLI IKTYLTYLLK ENTNILDMLG 1870 1880 1890 1900 1910 1920 HLFTSVQLIN TNLSSMKTSE IYVLFKNYKN RLTPCLQFDR NIILDNWSFF YINKPIREEF 1930 1940 1950 1960 1970 1980 ERARELLNED LGMGMPKELE PNPLIELSQM LQNSGVDGVA MSAISQEENL SFFSTKELAV 1990 2000 2010 2020 2030 2040 ICLIIISESH LVTTKKHKND CNCLQKKPYS DQEIKSWMSG IIGIGLYLSL LDNKTNSFEI 2050 2060 2070 2080 2090 2100 LDYLINSDFK SIHVNFNKER ELCWSINYSS NEKKGKDLWK KRFSVKDKMA FMGNWIRLLH 2110 2120 2130 2140 RQKVKNQKCE YKEGRINNFL KFINKGLNLQ KVKSQYEEEI AKLL

References

[1]

"RNA polymerase (L) gene and genome terminal sequences of ephemeroviruses bovine ephemeral fever virus and Adelaide River virus indicate a close relationship to vesiculoviruses." Dhillon J., Cowley J.A., Wang Y., Walker P.J. Virus Res. 70:87-95(2000) [PubMed: 11074128] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF234533 Genomic DNA. Translation: AAG10420.1.
RefSeq	NP_065409.1. NC_002526.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	911734.
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. IPR017234. RNA-dir_pol_rhabdovirus. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF037546. RNA_pol_RhabdoV_sub. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_BEFVB

Accession

Primary (citable) accession number: Q9E784

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program