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Q9E6N3

- DEN_GAHVM

UniProt

Q9E6N3 - DEN_GAHVM

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Protein

Deneddylase

Gene
MDV049
Organism
Gallid herpesvirus 2 (strain Chicken/Md5/ATCC VR-987) (GaHV-2) (Marek's disease herpesvirus type 1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei85 – 851Important for catalytic activity By similarity
Active sitei98 – 981 By similarity
Active sitei232 – 2321 By similarity
Active sitei234 – 2341 By similarity

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: InterPro
  2. omega peptidase activity Source: InterPro

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Gene namesi
Name:MDV049
OrganismiGallid herpesvirus 2 (strain Chicken/Md5/ATCC VR-987) (GaHV-2) (Marek's disease herpesvirus type 1)
Taxonomic identifieri10389 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeMardivirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]
ProteomesiUP000008072: Genome

Subcellular locationi

Virion tegument Reviewed prediction. Host nucleus By similarity

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-SubCell
  2. viral tegument Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 33423342DeneddylasePRO_0000406498Add
BLAST

Proteomic databases

PRIDEiQ9E6N3.

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9E6N3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini78 – 298221Peptidase C76Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili978 – 100831 Reviewed predictionAdd
BLAST
Coiled coili1779 – 180931 Reviewed predictionAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi490 – 4956Poly-Ser
Compositional biasi1212 – 12154Poly-Ala
Compositional biasi2296 – 22994Poly-Thr
Compositional biasi2676 – 2932257Pro-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9E6N3-1 [UniParc]FASTAAdd to Basket

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MTDSTDSRQA TTNCRKYSRT TSNAPMLAAN VLRDKSTSGL CSLDRKHDPY     50
FGQIMDNPEV ILDEWAKMVI DTTDVTVVAV GIRNQFAPDL SPASSVSCLR 100
SSLAFLRIVF AYGLDTVISS DAIDRLLLQG KAWTIATSED GTYTTCVPHD 150
LPNRIISKDA GGNLCVAFSS SYGEFEFYLE ENTPTILDTQ ISARTFIEQI 200
WKKKRGDVYC LIVVGVLGIG VYRSGDGIYI FDPHGHGHIG QACIVRVSEG 250
YFYQYLTSYA DPSATPDWSA TFVYFVSTVS ICPPRDEIIS TVSRIYGTSD 300
IVLDLGRARE EDNRKVVSAD FDPPSRPQPR LTKLVIGSTD TTIQGSDYPC 350
IQAEDLNGED SRPLDHNLSY NDAPTNTESV APPSTKDCRD CINIQNPGET 400
LDDTHSTIVD PSTKDSISVT AWQGVFSDVI EDPPPKTNFQ FSFGTFAKVA 450
ENKIGTTSVE GCIRNYARHK RRRPLWTPQS SSENISLDGS SSSLSRKHSR 500
KSKRTLESRI VEAVTSTESS DVTENVDTYP PVISNIPDEP TLGSSPTTSN 550
RGEDTTVEHL LKNRPLFNFK SLTEDEDGLV QDRLWSDEYL SHYPLADIRD 600
KIEDVACSID SGLRIIVHVG SPYDSDGGLL YVCMMDIFAR LFNYIIENGA 650
RTTSDRESVV GYEMAALLKA FTIPVYFTTF IASTGMVLSE ASESIDLIER 700
VLAENSKIGN LALSKMILVA LEVEEVTDEL HKSLDAIEKE VGTADPYGIY 750
ERMAAILVDT LYHNSGKLYS EKTSSNSNQT LTDRVISLCT LIRDIEAVAI 800
RKAELILAEM EALEAGVRWM NTTLDAFIMG GSGSSPMIDA ADIVAKTSSA 850
VVTQRLGDIG KTVIDVVGHS LREYYLKVAL YSVKALTASS SDVSRFKIVV 900
TDQYEKINRF ASSLSVIDDV MVLIASRSNA RVPSPVSQAF ESELLGNLLE 950
IGSDLDVPEK LTTWKNLMTS MQVGGWISRR ELDMLMKEID IVNEKATRHE 1000
TVLTELERLN ELETRFGSYT DLDTTVELQK LDEAIKIGED IVKLAIVLED 1050
KKNATSLSSD VREKLRDTRR KNETFITHLR ERYQEVKSTI EDLYSSIRKI 1100
LRPLPKFVGL RALDSKVKVI TESIPRGMGS FENFLASAPS DIIGSLQSDL 1150
WVLFIQYKTI LSRPTTEVAA ELSGLGVPFA LAIRLVFGPQ GSYPAASVFF 1200
GKHADVLSAT IAAAAVEPMS VEKTMAVVST LKAAISDIDR ANAIAPPQGI 1250
MSISSTERSD AFLFLKALLS TAEIAADVAN RGQHLESLIQ SVRTILDNLI 1300
TSNHKIRSLN PREVISENDT SVVASAKVEF SNAIQTVGNV TATLSTFEGL 1350
TYTSNPHIQR KIAELSKLIR SANQRAGELD IAIQTYEHNR ISAERSRSED 1400
LWISSINSLL LNAEVKSEFD AMEINRLEDA ARTGGYDTIR YKSRAEKIVI 1450
AHARVLESSI ESVLKFNPYS TQNMVHGLSP PIAALKSITW GDSFMTAAPY 1500
YTKLFGVNCD TVMDLLHISI AILRHANANS GNVDYYLLMG ELESALKSYP 1550
NLVKYVNFYR SGYVKFMSFL AHLEQRRVEA HHASGRVALE ISAALEDLAR 1600
THSPEGARRA LEYGVSIIIP SVNTIMSIAE ELKKDHVEEL EGTAYSEYGA 1650
HLLRRDTDAM TSLIHRVTTA IEDAKTRGEA ILKNLAEASY AADRESAELL 1700
ANLKNLLRLV SMPSHIAKAI DRSETVNDIV TQAALLLTKV EETKELDSQT 1750
VEWLRHAESV IDSHDLTVRI DESGPMSIYA DRIDALVNLS KRLEELKSEL 1800
ALAEVAWDDT WATFVHDKDR IDKSSEGFSS ARESAARTKV SVNMINALRS 1850
NAEYPRLPAK IIGLIDTKYR DRVVVLDAFL TTVKEIEATQ KQMEGLCEKI 1900
PSTFAINDLK KIYTQFEDIA KRLPKWYTKR VARYSRLLTL RLALYAGYSN 1950
TFEGNVGDPM LLPFDAGDAN NGANHTSNVG VVNRYLKHRV ASWIRPKVVA 2000
TLQEAFSEID SPGCLTYLDS TDKPLRYSLC FRTVGEKLAA CLCEPAAIGI 2050
KPQIPIQPIT TEETEAAAGM LSDIMTFRLG FVHDMANHLY SFTKYVRTKR 2100
HNWMQSDYIK ALGTIYCALI AITLTRKNRS NLSDIYFIPG RRTPIVDKKE 2150
LKKNAANGRG KQVVRLDPAD VMVTIMANIP GHMLTFSKLD LIDQYDFMDK 2200
TIYEVMTDSI STVAFVNCLS VQLSKDNIPD PNCRPLSLTG SVWDPAGGSL 2250
FSVRYSDWRQ GKLSDTDPLK LWEDLDGDAA DGLAKIRAAI PSSLLTTTTV 2300
LARMCIPPTA LAVIWSSLLP DGLEQNCKSY DDVVTARGDL ASSLDVTTSL 2350
LSCSENKNIS SITDSPNLYD LTGNVTTFTV VSTPPSRVLR VNAMDIATTA 2400
TLFGARIVIA AECPEAYSSE SGLSLCIRLF DSRHGSRGCF LEPTAVSSDM 2450
TSWGTKLLIT DNNPIENACL GQQLEHLSRI VASKPLASAP PCLLIVDSGM 2500
APIKVLWSKE ILDPIPIIRL ISEDDALISE LPYVDAGIRK EPELANEHMV 2550
IADVQEASKF FSDESRIYPC PIYNKCVSPD LSRDGDADIS SNRIDSEDDT 2600
YADSMGSGYL SIDPESMWDR VVENDMEGAQ VQLLLPGDII HHNDRHVSEN 2650
MNYPQIGHLD ISPNYRDPID ETSSNPPPFP KHSNLFPSDH DPTSESSSKP 2700
TPAPKPTPAP KPTPAPKPTP APKPTPAPKP TPAPKPTPAP KPKPPPDPDF 2750
KPSPAPKPSP ASKPTPAPKP SPAPKPKPPP DPDFKPTPAP KPSPASKPKP 2800
PPDPDFKPTP APKPKPPPDP DFKPSPAPKP SPAPKPKPPP DPDFKPTPAP 2850
KPSPASKPSP ASKPKPPPAP DSKPSPAPKP KPPPTPDSKP SPAPKPKSPS 2900
ASKPLPVLFP NSDSKTSPVP NPNTFSASKI PPTSSIAEET KPCQSNLPAI 2950
PLITKPDSVK NGYTTLKTDD KRKGSQSRYR NEKSRKHMHN THTAVYNTTF 3000
NWTGTAAASS RHDMELNQYS PGIVTFDNLI DKEGYRHESE TIPRESYSEH 3050
RKDLDWMSTP TVIANASSSL ITNNDYGGIG GIDLKKYRFK NGTGLLRQGS 3100
PTTRLHCRKN NSSRSITDIL VGTASPTNEP SPLLQRLKAH TISGDKKANM 3150
DAGTIRGRLY DYSSFWPPCI QGACSTSPKT IDLKYLSSEQ ATVAYPKHDD 3200
THHQTPTGLA PNDKHSDMHI SSIITETDTK ENSIPGYNNI PMRHSSESES 3250
LTSLDSDSDD SHLHVSGSTD TTTDGSSTSR VIPADALLTR RDFRNASRGA 3300
LYALTKACKK VARQIVYVRE QLRTKVATLA IELFKIKMIL TG 3342
Length:3,342
Mass (Da):367,314
Last modified:March 1, 2001 - v1
Checksum:i49B6E04418E54890
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF243438 Genomic DNA. Translation: AAG14229.1.
RefSeqiYP_001033965.1. NC_002229.3.

Genome annotation databases

GeneIDi4811510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF243438 Genomic DNA. Translation: AAG14229.1 .
RefSeqi YP_001033965.1. NC_002229.3.

3D structure databases

ProteinModelPortali Q9E6N3.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9E6N3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4811510.

Family and domain databases

InterProi IPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of a very virulent Marek's disease virus."
    Tulman E.R., Afonso C.L., Lu Z., Zsak L., Rock D.L., Kutish G.F.
    J. Virol. 74:7980-7988(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDEN_GAHVM
AccessioniPrimary (citable) accession number: Q9E6N3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi