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Q9E6N3

- DEN_GAHVM

UniProt

Q9E6N3 - DEN_GAHVM

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Protein

Deneddylase

Gene

MDV049

Organism
Gallid herpesvirus 2 (strain Chicken/Md5/ATCC VR-987) (GaHV-2) (Marek's disease herpesvirus type 1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei85 – 851Important for catalytic activityPROSITE-ProRule annotation
Active sitei98 – 981PROSITE-ProRule annotation
Active sitei232 – 2321PROSITE-ProRule annotation
Active sitei234 – 2341PROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: InterPro
  2. omega peptidase activity Source: InterPro

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Gene namesi
Name:MDV049
OrganismiGallid herpesvirus 2 (strain Chicken/Md5/ATCC VR-987) (GaHV-2) (Marek's disease herpesvirus type 1)
Taxonomic identifieri10389 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeMardivirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]
ProteomesiUP000008072: Genome

Subcellular locationi

Virion tegument Curated. Host nucleus By similarity

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-KW
  2. viral tegument Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 33423342DeneddylasePRO_0000406498Add
BLAST

Proteomic databases

PRIDEiQ9E6N3.

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9E6N3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini78 – 298221Peptidase C76PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili978 – 100831Sequence AnalysisAdd
BLAST
Coiled coili1779 – 180931Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi490 – 4956Poly-Ser
Compositional biasi1212 – 12154Poly-Ala
Compositional biasi2296 – 22994Poly-Thr
Compositional biasi2676 – 2932257Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the herpesviridae deneddylase family.Curated
Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9E6N3-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MTDSTDSRQA TTNCRKYSRT TSNAPMLAAN VLRDKSTSGL CSLDRKHDPY
60 70 80 90 100
FGQIMDNPEV ILDEWAKMVI DTTDVTVVAV GIRNQFAPDL SPASSVSCLR
110 120 130 140 150
SSLAFLRIVF AYGLDTVISS DAIDRLLLQG KAWTIATSED GTYTTCVPHD
160 170 180 190 200
LPNRIISKDA GGNLCVAFSS SYGEFEFYLE ENTPTILDTQ ISARTFIEQI
210 220 230 240 250
WKKKRGDVYC LIVVGVLGIG VYRSGDGIYI FDPHGHGHIG QACIVRVSEG
260 270 280 290 300
YFYQYLTSYA DPSATPDWSA TFVYFVSTVS ICPPRDEIIS TVSRIYGTSD
310 320 330 340 350
IVLDLGRARE EDNRKVVSAD FDPPSRPQPR LTKLVIGSTD TTIQGSDYPC
360 370 380 390 400
IQAEDLNGED SRPLDHNLSY NDAPTNTESV APPSTKDCRD CINIQNPGET
410 420 430 440 450
LDDTHSTIVD PSTKDSISVT AWQGVFSDVI EDPPPKTNFQ FSFGTFAKVA
460 470 480 490 500
ENKIGTTSVE GCIRNYARHK RRRPLWTPQS SSENISLDGS SSSLSRKHSR
510 520 530 540 550
KSKRTLESRI VEAVTSTESS DVTENVDTYP PVISNIPDEP TLGSSPTTSN
560 570 580 590 600
RGEDTTVEHL LKNRPLFNFK SLTEDEDGLV QDRLWSDEYL SHYPLADIRD
610 620 630 640 650
KIEDVACSID SGLRIIVHVG SPYDSDGGLL YVCMMDIFAR LFNYIIENGA
660 670 680 690 700
RTTSDRESVV GYEMAALLKA FTIPVYFTTF IASTGMVLSE ASESIDLIER
710 720 730 740 750
VLAENSKIGN LALSKMILVA LEVEEVTDEL HKSLDAIEKE VGTADPYGIY
760 770 780 790 800
ERMAAILVDT LYHNSGKLYS EKTSSNSNQT LTDRVISLCT LIRDIEAVAI
810 820 830 840 850
RKAELILAEM EALEAGVRWM NTTLDAFIMG GSGSSPMIDA ADIVAKTSSA
860 870 880 890 900
VVTQRLGDIG KTVIDVVGHS LREYYLKVAL YSVKALTASS SDVSRFKIVV
910 920 930 940 950
TDQYEKINRF ASSLSVIDDV MVLIASRSNA RVPSPVSQAF ESELLGNLLE
960 970 980 990 1000
IGSDLDVPEK LTTWKNLMTS MQVGGWISRR ELDMLMKEID IVNEKATRHE
1010 1020 1030 1040 1050
TVLTELERLN ELETRFGSYT DLDTTVELQK LDEAIKIGED IVKLAIVLED
1060 1070 1080 1090 1100
KKNATSLSSD VREKLRDTRR KNETFITHLR ERYQEVKSTI EDLYSSIRKI
1110 1120 1130 1140 1150
LRPLPKFVGL RALDSKVKVI TESIPRGMGS FENFLASAPS DIIGSLQSDL
1160 1170 1180 1190 1200
WVLFIQYKTI LSRPTTEVAA ELSGLGVPFA LAIRLVFGPQ GSYPAASVFF
1210 1220 1230 1240 1250
GKHADVLSAT IAAAAVEPMS VEKTMAVVST LKAAISDIDR ANAIAPPQGI
1260 1270 1280 1290 1300
MSISSTERSD AFLFLKALLS TAEIAADVAN RGQHLESLIQ SVRTILDNLI
1310 1320 1330 1340 1350
TSNHKIRSLN PREVISENDT SVVASAKVEF SNAIQTVGNV TATLSTFEGL
1360 1370 1380 1390 1400
TYTSNPHIQR KIAELSKLIR SANQRAGELD IAIQTYEHNR ISAERSRSED
1410 1420 1430 1440 1450
LWISSINSLL LNAEVKSEFD AMEINRLEDA ARTGGYDTIR YKSRAEKIVI
1460 1470 1480 1490 1500
AHARVLESSI ESVLKFNPYS TQNMVHGLSP PIAALKSITW GDSFMTAAPY
1510 1520 1530 1540 1550
YTKLFGVNCD TVMDLLHISI AILRHANANS GNVDYYLLMG ELESALKSYP
1560 1570 1580 1590 1600
NLVKYVNFYR SGYVKFMSFL AHLEQRRVEA HHASGRVALE ISAALEDLAR
1610 1620 1630 1640 1650
THSPEGARRA LEYGVSIIIP SVNTIMSIAE ELKKDHVEEL EGTAYSEYGA
1660 1670 1680 1690 1700
HLLRRDTDAM TSLIHRVTTA IEDAKTRGEA ILKNLAEASY AADRESAELL
1710 1720 1730 1740 1750
ANLKNLLRLV SMPSHIAKAI DRSETVNDIV TQAALLLTKV EETKELDSQT
1760 1770 1780 1790 1800
VEWLRHAESV IDSHDLTVRI DESGPMSIYA DRIDALVNLS KRLEELKSEL
1810 1820 1830 1840 1850
ALAEVAWDDT WATFVHDKDR IDKSSEGFSS ARESAARTKV SVNMINALRS
1860 1870 1880 1890 1900
NAEYPRLPAK IIGLIDTKYR DRVVVLDAFL TTVKEIEATQ KQMEGLCEKI
1910 1920 1930 1940 1950
PSTFAINDLK KIYTQFEDIA KRLPKWYTKR VARYSRLLTL RLALYAGYSN
1960 1970 1980 1990 2000
TFEGNVGDPM LLPFDAGDAN NGANHTSNVG VVNRYLKHRV ASWIRPKVVA
2010 2020 2030 2040 2050
TLQEAFSEID SPGCLTYLDS TDKPLRYSLC FRTVGEKLAA CLCEPAAIGI
2060 2070 2080 2090 2100
KPQIPIQPIT TEETEAAAGM LSDIMTFRLG FVHDMANHLY SFTKYVRTKR
2110 2120 2130 2140 2150
HNWMQSDYIK ALGTIYCALI AITLTRKNRS NLSDIYFIPG RRTPIVDKKE
2160 2170 2180 2190 2200
LKKNAANGRG KQVVRLDPAD VMVTIMANIP GHMLTFSKLD LIDQYDFMDK
2210 2220 2230 2240 2250
TIYEVMTDSI STVAFVNCLS VQLSKDNIPD PNCRPLSLTG SVWDPAGGSL
2260 2270 2280 2290 2300
FSVRYSDWRQ GKLSDTDPLK LWEDLDGDAA DGLAKIRAAI PSSLLTTTTV
2310 2320 2330 2340 2350
LARMCIPPTA LAVIWSSLLP DGLEQNCKSY DDVVTARGDL ASSLDVTTSL
2360 2370 2380 2390 2400
LSCSENKNIS SITDSPNLYD LTGNVTTFTV VSTPPSRVLR VNAMDIATTA
2410 2420 2430 2440 2450
TLFGARIVIA AECPEAYSSE SGLSLCIRLF DSRHGSRGCF LEPTAVSSDM
2460 2470 2480 2490 2500
TSWGTKLLIT DNNPIENACL GQQLEHLSRI VASKPLASAP PCLLIVDSGM
2510 2520 2530 2540 2550
APIKVLWSKE ILDPIPIIRL ISEDDALISE LPYVDAGIRK EPELANEHMV
2560 2570 2580 2590 2600
IADVQEASKF FSDESRIYPC PIYNKCVSPD LSRDGDADIS SNRIDSEDDT
2610 2620 2630 2640 2650
YADSMGSGYL SIDPESMWDR VVENDMEGAQ VQLLLPGDII HHNDRHVSEN
2660 2670 2680 2690 2700
MNYPQIGHLD ISPNYRDPID ETSSNPPPFP KHSNLFPSDH DPTSESSSKP
2710 2720 2730 2740 2750
TPAPKPTPAP KPTPAPKPTP APKPTPAPKP TPAPKPTPAP KPKPPPDPDF
2760 2770 2780 2790 2800
KPSPAPKPSP ASKPTPAPKP SPAPKPKPPP DPDFKPTPAP KPSPASKPKP
2810 2820 2830 2840 2850
PPDPDFKPTP APKPKPPPDP DFKPSPAPKP SPAPKPKPPP DPDFKPTPAP
2860 2870 2880 2890 2900
KPSPASKPSP ASKPKPPPAP DSKPSPAPKP KPPPTPDSKP SPAPKPKSPS
2910 2920 2930 2940 2950
ASKPLPVLFP NSDSKTSPVP NPNTFSASKI PPTSSIAEET KPCQSNLPAI
2960 2970 2980 2990 3000
PLITKPDSVK NGYTTLKTDD KRKGSQSRYR NEKSRKHMHN THTAVYNTTF
3010 3020 3030 3040 3050
NWTGTAAASS RHDMELNQYS PGIVTFDNLI DKEGYRHESE TIPRESYSEH
3060 3070 3080 3090 3100
RKDLDWMSTP TVIANASSSL ITNNDYGGIG GIDLKKYRFK NGTGLLRQGS
3110 3120 3130 3140 3150
PTTRLHCRKN NSSRSITDIL VGTASPTNEP SPLLQRLKAH TISGDKKANM
3160 3170 3180 3190 3200
DAGTIRGRLY DYSSFWPPCI QGACSTSPKT IDLKYLSSEQ ATVAYPKHDD
3210 3220 3230 3240 3250
THHQTPTGLA PNDKHSDMHI SSIITETDTK ENSIPGYNNI PMRHSSESES
3260 3270 3280 3290 3300
LTSLDSDSDD SHLHVSGSTD TTTDGSSTSR VIPADALLTR RDFRNASRGA
3310 3320 3330 3340
LYALTKACKK VARQIVYVRE QLRTKVATLA IELFKIKMIL TG
Length:3,342
Mass (Da):367,314
Last modified:March 1, 2001 - v1
Checksum:i49B6E04418E54890
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF243438 Genomic DNA. Translation: AAG14229.1.
RefSeqiYP_001033965.1. NC_002229.3.

Genome annotation databases

GeneIDi4811510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF243438 Genomic DNA. Translation: AAG14229.1 .
RefSeqi YP_001033965.1. NC_002229.3.

3D structure databases

ProteinModelPortali Q9E6N3.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9E6N3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4811510.

Family and domain databases

InterProi IPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of a very virulent Marek's disease virus."
    Tulman E.R., Afonso C.L., Lu Z., Zsak L., Rock D.L., Kutish G.F.
    J. Virol. 74:7980-7988(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDEN_GAHVM
AccessioniPrimary (citable) accession number: Q9E6N3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3