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Q9E6N3 (DEN_GAHVM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length3342 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Subcellular location

Virion tegument Potential. Host nucleus By similarity.

Sequence similarities

Belongs to the herpesviridae deneddylase family.

Contains 1 peptidase C76 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 33423342Deneddylase
PRO_0000406498

Regions

Domain78 – 298221Peptidase C76
Coiled coil978 – 100831 Potential
Coiled coil1779 – 180931 Potential
Compositional bias490 – 4956Poly-Ser
Compositional bias1212 – 12154Poly-Ala
Compositional bias2296 – 22994Poly-Thr
Compositional bias2676 – 2932257Pro-rich

Sites

Active site981 By similarity
Active site2321 By similarity
Active site2341 By similarity
Site851Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9E6N3 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 49B6E04418E54890

FASTA3,342367,314
        10         20         30         40         50         60 
MTDSTDSRQA TTNCRKYSRT TSNAPMLAAN VLRDKSTSGL CSLDRKHDPY FGQIMDNPEV 

        70         80         90        100        110        120 
ILDEWAKMVI DTTDVTVVAV GIRNQFAPDL SPASSVSCLR SSLAFLRIVF AYGLDTVISS 

       130        140        150        160        170        180 
DAIDRLLLQG KAWTIATSED GTYTTCVPHD LPNRIISKDA GGNLCVAFSS SYGEFEFYLE 

       190        200        210        220        230        240 
ENTPTILDTQ ISARTFIEQI WKKKRGDVYC LIVVGVLGIG VYRSGDGIYI FDPHGHGHIG 

       250        260        270        280        290        300 
QACIVRVSEG YFYQYLTSYA DPSATPDWSA TFVYFVSTVS ICPPRDEIIS TVSRIYGTSD 

       310        320        330        340        350        360 
IVLDLGRARE EDNRKVVSAD FDPPSRPQPR LTKLVIGSTD TTIQGSDYPC IQAEDLNGED 

       370        380        390        400        410        420 
SRPLDHNLSY NDAPTNTESV APPSTKDCRD CINIQNPGET LDDTHSTIVD PSTKDSISVT 

       430        440        450        460        470        480 
AWQGVFSDVI EDPPPKTNFQ FSFGTFAKVA ENKIGTTSVE GCIRNYARHK RRRPLWTPQS 

       490        500        510        520        530        540 
SSENISLDGS SSSLSRKHSR KSKRTLESRI VEAVTSTESS DVTENVDTYP PVISNIPDEP 

       550        560        570        580        590        600 
TLGSSPTTSN RGEDTTVEHL LKNRPLFNFK SLTEDEDGLV QDRLWSDEYL SHYPLADIRD 

       610        620        630        640        650        660 
KIEDVACSID SGLRIIVHVG SPYDSDGGLL YVCMMDIFAR LFNYIIENGA RTTSDRESVV 

       670        680        690        700        710        720 
GYEMAALLKA FTIPVYFTTF IASTGMVLSE ASESIDLIER VLAENSKIGN LALSKMILVA 

       730        740        750        760        770        780 
LEVEEVTDEL HKSLDAIEKE VGTADPYGIY ERMAAILVDT LYHNSGKLYS EKTSSNSNQT 

       790        800        810        820        830        840 
LTDRVISLCT LIRDIEAVAI RKAELILAEM EALEAGVRWM NTTLDAFIMG GSGSSPMIDA 

       850        860        870        880        890        900 
ADIVAKTSSA VVTQRLGDIG KTVIDVVGHS LREYYLKVAL YSVKALTASS SDVSRFKIVV 

       910        920        930        940        950        960 
TDQYEKINRF ASSLSVIDDV MVLIASRSNA RVPSPVSQAF ESELLGNLLE IGSDLDVPEK 

       970        980        990       1000       1010       1020 
LTTWKNLMTS MQVGGWISRR ELDMLMKEID IVNEKATRHE TVLTELERLN ELETRFGSYT 

      1030       1040       1050       1060       1070       1080 
DLDTTVELQK LDEAIKIGED IVKLAIVLED KKNATSLSSD VREKLRDTRR KNETFITHLR 

      1090       1100       1110       1120       1130       1140 
ERYQEVKSTI EDLYSSIRKI LRPLPKFVGL RALDSKVKVI TESIPRGMGS FENFLASAPS 

      1150       1160       1170       1180       1190       1200 
DIIGSLQSDL WVLFIQYKTI LSRPTTEVAA ELSGLGVPFA LAIRLVFGPQ GSYPAASVFF 

      1210       1220       1230       1240       1250       1260 
GKHADVLSAT IAAAAVEPMS VEKTMAVVST LKAAISDIDR ANAIAPPQGI MSISSTERSD 

      1270       1280       1290       1300       1310       1320 
AFLFLKALLS TAEIAADVAN RGQHLESLIQ SVRTILDNLI TSNHKIRSLN PREVISENDT 

      1330       1340       1350       1360       1370       1380 
SVVASAKVEF SNAIQTVGNV TATLSTFEGL TYTSNPHIQR KIAELSKLIR SANQRAGELD 

      1390       1400       1410       1420       1430       1440 
IAIQTYEHNR ISAERSRSED LWISSINSLL LNAEVKSEFD AMEINRLEDA ARTGGYDTIR 

      1450       1460       1470       1480       1490       1500 
YKSRAEKIVI AHARVLESSI ESVLKFNPYS TQNMVHGLSP PIAALKSITW GDSFMTAAPY 

      1510       1520       1530       1540       1550       1560 
YTKLFGVNCD TVMDLLHISI AILRHANANS GNVDYYLLMG ELESALKSYP NLVKYVNFYR 

      1570       1580       1590       1600       1610       1620 
SGYVKFMSFL AHLEQRRVEA HHASGRVALE ISAALEDLAR THSPEGARRA LEYGVSIIIP 

      1630       1640       1650       1660       1670       1680 
SVNTIMSIAE ELKKDHVEEL EGTAYSEYGA HLLRRDTDAM TSLIHRVTTA IEDAKTRGEA 

      1690       1700       1710       1720       1730       1740 
ILKNLAEASY AADRESAELL ANLKNLLRLV SMPSHIAKAI DRSETVNDIV TQAALLLTKV 

      1750       1760       1770       1780       1790       1800 
EETKELDSQT VEWLRHAESV IDSHDLTVRI DESGPMSIYA DRIDALVNLS KRLEELKSEL 

      1810       1820       1830       1840       1850       1860 
ALAEVAWDDT WATFVHDKDR IDKSSEGFSS ARESAARTKV SVNMINALRS NAEYPRLPAK 

      1870       1880       1890       1900       1910       1920 
IIGLIDTKYR DRVVVLDAFL TTVKEIEATQ KQMEGLCEKI PSTFAINDLK KIYTQFEDIA 

      1930       1940       1950       1960       1970       1980 
KRLPKWYTKR VARYSRLLTL RLALYAGYSN TFEGNVGDPM LLPFDAGDAN NGANHTSNVG 

      1990       2000       2010       2020       2030       2040 
VVNRYLKHRV ASWIRPKVVA TLQEAFSEID SPGCLTYLDS TDKPLRYSLC FRTVGEKLAA 

      2050       2060       2070       2080       2090       2100 
CLCEPAAIGI KPQIPIQPIT TEETEAAAGM LSDIMTFRLG FVHDMANHLY SFTKYVRTKR 

      2110       2120       2130       2140       2150       2160 
HNWMQSDYIK ALGTIYCALI AITLTRKNRS NLSDIYFIPG RRTPIVDKKE LKKNAANGRG 

      2170       2180       2190       2200       2210       2220 
KQVVRLDPAD VMVTIMANIP GHMLTFSKLD LIDQYDFMDK TIYEVMTDSI STVAFVNCLS 

      2230       2240       2250       2260       2270       2280 
VQLSKDNIPD PNCRPLSLTG SVWDPAGGSL FSVRYSDWRQ GKLSDTDPLK LWEDLDGDAA 

      2290       2300       2310       2320       2330       2340 
DGLAKIRAAI PSSLLTTTTV LARMCIPPTA LAVIWSSLLP DGLEQNCKSY DDVVTARGDL 

      2350       2360       2370       2380       2390       2400 
ASSLDVTTSL LSCSENKNIS SITDSPNLYD LTGNVTTFTV VSTPPSRVLR VNAMDIATTA 

      2410       2420       2430       2440       2450       2460 
TLFGARIVIA AECPEAYSSE SGLSLCIRLF DSRHGSRGCF LEPTAVSSDM TSWGTKLLIT 

      2470       2480       2490       2500       2510       2520 
DNNPIENACL GQQLEHLSRI VASKPLASAP PCLLIVDSGM APIKVLWSKE ILDPIPIIRL 

      2530       2540       2550       2560       2570       2580 
ISEDDALISE LPYVDAGIRK EPELANEHMV IADVQEASKF FSDESRIYPC PIYNKCVSPD 

      2590       2600       2610       2620       2630       2640 
LSRDGDADIS SNRIDSEDDT YADSMGSGYL SIDPESMWDR VVENDMEGAQ VQLLLPGDII 

      2650       2660       2670       2680       2690       2700 
HHNDRHVSEN MNYPQIGHLD ISPNYRDPID ETSSNPPPFP KHSNLFPSDH DPTSESSSKP 

      2710       2720       2730       2740       2750       2760 
TPAPKPTPAP KPTPAPKPTP APKPTPAPKP TPAPKPTPAP KPKPPPDPDF KPSPAPKPSP 

      2770       2780       2790       2800       2810       2820 
ASKPTPAPKP SPAPKPKPPP DPDFKPTPAP KPSPASKPKP PPDPDFKPTP APKPKPPPDP 

      2830       2840       2850       2860       2870       2880 
DFKPSPAPKP SPAPKPKPPP DPDFKPTPAP KPSPASKPSP ASKPKPPPAP DSKPSPAPKP 

      2890       2900       2910       2920       2930       2940 
KPPPTPDSKP SPAPKPKSPS ASKPLPVLFP NSDSKTSPVP NPNTFSASKI PPTSSIAEET 

      2950       2960       2970       2980       2990       3000 
KPCQSNLPAI PLITKPDSVK NGYTTLKTDD KRKGSQSRYR NEKSRKHMHN THTAVYNTTF 

      3010       3020       3030       3040       3050       3060 
NWTGTAAASS RHDMELNQYS PGIVTFDNLI DKEGYRHESE TIPRESYSEH RKDLDWMSTP 

      3070       3080       3090       3100       3110       3120 
TVIANASSSL ITNNDYGGIG GIDLKKYRFK NGTGLLRQGS PTTRLHCRKN NSSRSITDIL 

      3130       3140       3150       3160       3170       3180 
VGTASPTNEP SPLLQRLKAH TISGDKKANM DAGTIRGRLY DYSSFWPPCI QGACSTSPKT 

      3190       3200       3210       3220       3230       3240 
IDLKYLSSEQ ATVAYPKHDD THHQTPTGLA PNDKHSDMHI SSIITETDTK ENSIPGYNNI 

      3250       3260       3270       3280       3290       3300 
PMRHSSESES LTSLDSDSDD SHLHVSGSTD TTTDGSSTSR VIPADALLTR RDFRNASRGA 

      3310       3320       3330       3340 
LYALTKACKK VARQIVYVRE QLRTKVATLA IELFKIKMIL TG 

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References

[1]"The genome of a very virulent Marek's disease virus."
Tulman E.R., Afonso C.L., Lu Z., Zsak L., Rock D.L., Kutish G.F.
J. Virol. 74:7980-7988(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF243438 Genomic DNA. Translation: AAG14229.1.
RefSeqYP_001033965.1. NC_002229.3.

3D structure databases

ProteinModelPortalQ9E6N3.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9E6N3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4811510.

Family and domain databases

InterProIPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamPF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view]
PROSITEPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDEN_GAHVM
AccessionPrimary (citable) accession number: Q9E6N3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries