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Q9DUN3

- POLG_VESVA

UniProt

Q9DUN3 - POLG_VESVA

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Protein
Genome polyprotein
Gene
ORF1
Organism
Vesicular exanthema of swine virus serotype A48 (isolate Swine/United States/A48/1948) (VESV)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

NTPase presumably plays a role in replication. Despite having similarities with helicases, does not seem to display any helicase activity By similarity.
Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation By similarity.
Protease-polymerase p76 processes the polyprotein: Pro-Pol is first released by autocleavage, then all other proteins are cleaved. Cleaves host translation initiation factor eIF4G1 and eIF4G2 thereby inducing a shutdown of host protein synthesis. This shutdown may not prevent viral mRNA from being translated since viral Vpg replaces the cap. May cleave host polyadenylate-binding protein thereby inhibiting cellular translation. It is also an RNA-directed RNA polymerase which replicates genomic and antigenomic viral RNA by recognizing specific signals. Transcribes also a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This sgRNA codes for structural proteins. Catalyzes the covalent attachment VPg with viral RNAs By similarity.

Catalytic activityi

NTP + H2O = NDP + phosphate.
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei148 – 1492Cleavage; by Pro-Pol By similarity
Sitei435 – 4362Cleavage; by Pro-Pol By similarity
Sitei791 – 7922Cleavage; by Pro-Pol By similarity
Sitei1070 – 10712Cleavage; by Pro-Pol By similarity
Sitei1183 – 11842Cleavage; by Pro-Pol By similarity
Active sitei1222 – 12221For protease activity By similarity
Active sitei1243 – 12431For protease activity By similarity
Active sitei1305 – 13051For protease activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi590 – 5978ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: InterPro
  3. RNA helicase activity Source: InterPro
  4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  5. cysteine-type endopeptidase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. RNA-protein covalent cross-linking Source: UniProtKB-KW
  2. transcription, DNA-templated Source: InterPro
  3. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Cleaved into the following 6 chains:
Alternative name(s):
p39
Alternative name(s):
VPg
p13
Protease-polymerase p76 (EC:2.7.7.48, EC:3.4.22.66)
Short name:
Pro-Pol
Gene namesi
ORF Names:ORF1
OrganismiVesicular exanthema of swine virus serotype A48 (isolate Swine/United States/A48/1948) (VESV)
Taxonomic identifieri85617 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeVesivirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000007661: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18811881Genome polyprotein
PRO_0000402456Add
BLAST
Chaini1 – 148148Protein p16 By similarity
PRO_0000402457Add
BLAST
Chaini149 – 435287Protein p32 By similarity
PRO_0000402458Add
BLAST
Chaini436 – 791356NTPase By similarity
PRO_0000402459Add
BLAST
Chaini792 – 1070279Protein p30 By similarity
PRO_0000402460Add
BLAST
Chaini1071 – 1183113Viral genome-linked protein By similarity
PRO_0000402461Add
BLAST
Chaini1184 – 1881698Protease-polymerase p76 By similarity
PRO_0000402462Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1093 – 10931O-(5'-phospho-RNA)-tyrosine By similarity

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins. Pro-Pol is first autocatalytically cleaved, then processes the whole polyprotein By similarity.
VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.

Keywords - PTMi

Covalent protein-RNA linkage, Phosphoprotein

Interactioni

Subunit structurei

Protein p32: homodimer, interacts with NTPase, protein p30 and Pro-Pol. Viral genome-linked protein interacts with capsid protein and Pro-Pol. Protease-polymerase p76: Homooligomers, interacts with Vpg, protein p32 and may interact with capsid protein By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9DUN3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini564 – 720157SF3 helicase
Add
BLAST
Domaini1207 – 1311105Peptidase C24
Add
BLAST
Domaini1593 – 1718126RdRp catalytic
Add
BLAST

Domaini

Protease-polymerase is composed of two domains displaying two different catalytic activity. These activities may act independently By similarity.

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.300. 6 hits.
InterProiIPR003593. AAA+_ATPase.
IPR016024. ARM-type_fold.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000317. Peptidase_C24.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF03510. Peptidase_C24. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00916. 2CENDOPTASE.
PR00918. CALICVIRUSNS.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DUN3-1 [UniParc]FASTAAdd to Basket

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MAQTLSKISN KENASSGLRP KRFKPHQPIP TWMVRCEPLD HDSRRGRDPV     50
RASPQAKRVR TPTPYPRHLK PAASAVVRSG NNPSHLKPAS TDVVRSGPQP 100
LCCEAKDGGV VRSCKTYNLK PAHESKAVAF SLPKTDGPTG NEPEFIAEAC 150
PSCALYDTCP NCTSKVINDD GSTDGTIPSW DQIETTPAFL SLLSNTDEEM 200
SADELTNLAA HLRKAFETGS HPANVDYSKD QLQGLLEMAE AAVPPARRQT 250
LPFYQQRLEA RRTWREKIFN QPLEEINKIL TTSKDRFQRC AAWKVILEKA 300
VLAKEYGEEA YAYAQQALKN INSFDVNLVL KMAAATFIDH IRMMTVDNPD 350
LVSYIPKLIV KLKPLTLKMI IDNHENTKEG WLVTLTSLAE LYGMVEVAID 400
FVPTVVGKLF DLLMKTTSKM YSMFKSVVLA TFTSESLDFT NPFWYAIAAI 450
LCFLITGAIP HNGKMKIHKN ILSNATGIVA GIKAIQALAA MFSTWSNERL 500
VNDLSSRTIA LTELNNPTIT ADIDAVINLQ RLAEVLRDEV KSHTLNPLMQ 550
PYNPILRNLM SALDKVISCC TRRKAIATKR TAPVAVILTG PPGCGKTTAA 600
FALAKRLSQQ KPSIISLDVD HHDTYTGNEV CIIDEFDSSD KVDYANFVVN 650
MVNTNPMVLN CDLIENKGKT FTSKYVIMTS NTETPVKPTS RRAGAFYRRV 700
MIVDVTNNAV DKWKSDNPGK AVPKWCFNKD FSHLSLSLRG TEPYSKEYVL 750
DPTGRNHQSR RAPPPQQITL EQLAQKMVVQ HTTNTSEFVT QAGDVPVFGF 800
VCQNNEIDTV YNLLAAVKAR YGANFNLYKG MVRTAHENSG CGAHVHVISR 850
EDNFRGKAFT VNRSRLESVP HLEGDSFRRS LGVVMSDKDV TTMFYYIKGK 900
VINDQVSLTE LPANQHVVTV HTVYDMAWAL RRHLKWSGQW QLIKAAYEIM 950
CYPDTAACAL RNWMDSTDFS EEHVVTQFIA PGGTIILESC YGARMWATGQ 1000
RLIRAGGLTE AGGPQGGVRF AGLGARNVPW SEILREFMTL ISHIWSQIKG 1050
ATVVLTALTF YLKRFRPRVE AKGKNKNKGP RKNTGVALTD DEYNDWKQSK 1100
AEKNLDLTVK DFLQLRHRAA MGADNTDAVK FRYWYSKKQK IYHDLENFPI 1150
IGRGGLKREL IRKGPLRPRG NDFYDEPDDW YSEGVIDGVT HKNAIVSVDD 1200
VDGMHKGYAI HIGHGVYISL KHVLTGNARI LSEEPKGITI SGELATFRLN 1250
NILPTAVPVG TNKPIKDPWG NPVSTDWQFK NYNTTSGNIY GACGSSCSLT 1300
RQGDCGLPYV DDHGVVVGLH AGSGGDKCPS RKLIVPYVKV DMRIRDTCTK 1350
ESPTKTHKPT FSYRGLLGKE TGEPRTIMKG TRLHVSPAHV DDYEECTHQP 1400
ASLGAGDPRC PISLTGIMVN NLQPYTEASP GPDTATLNRV SKMLTSHMEG 1450
YVPKVHKTEE DSISAFYMLN HDTLCGPYIG ARKKDHVKDG VLDKNLLDLL 1500
SSKWNRAKLG LALPHEYALG LKDELRPKDK VAVGKRRLIW GCDVGVSTVC 1550
AAAFKRVSES IMANHALGFI QVGINMDGPA VEDLFKRLER PKHDRYCVDY 1600
SKWDSTQPPK VTSQSIDILR HFTDKSPIVD SACATLKSNP IGIFNGVAFK 1650
VAGGLPSGMP LTSIINSLNH CLMVGSAVVK ALEDSGVRVT WNIFDSMDLF 1700
TYGDDGVYIV PPLISSVMPK VFANLRQFGL KPTRTDKSDA EITPIPADEP 1750
VEFLKRTIVR TENGVRALLD RSSIIRQFYY IKAENTENWT VPPKRIDTPS 1800
RGQQLYNACL YASQHGEEFY TSKIVPLIER AVKLEGLHIE VPEFHQAVAA 1850
YNGYFNGTEG QPNQIAHASG GLGLSGEVFE N 1881
Length:1,881
Mass (Da):208,813
Last modified:March 1, 2001 - v1
Checksum:i6E487A72523AFE4F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U76874 Genomic RNA. Translation: AAG13641.1.
RefSeqiNP_066255.1. NC_002551.1.

Genome annotation databases

GeneIDi911834.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U76874 Genomic RNA. Translation: AAG13641.1 .
RefSeqi NP_066255.1. NC_002551.1.

3D structure databases

ProteinModelPortali Q9DUN3.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 911834.

Family and domain databases

Gene3Di 3.40.50.300. 6 hits.
InterProi IPR003593. AAA+_ATPase.
IPR016024. ARM-type_fold.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000317. Peptidase_C24.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF03510. Peptidase_C24. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
PRINTSi PR00916. 2CENDOPTASE.
PR00918. CALICVIRUSNS.
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 3 hits.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The capsid protein of vesicular exanthema of swine virus serotype A48: relationship to the capsid protein of other animal caliciviruses."
    Neill J.D., Meyer R.F., Seal B.S.
    Virus Res. 54:39-50(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Complete nucleotide sequence of the genomic RNA of vesicular exanthema of swine virus A48."
    Neill J.D., Seal B.S., Ridpath J.F.
    Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiPOLG_VESVA
AccessioniPrimary (citable) accession number: Q9DUN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi