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Q9DSN9

- POLN_ABPVR

UniProt

Q9DSN9 - POLN_ABPVR

Protein

Replicase polyprotein

Gene

ORF1

Organism
Acute bee paralysis virus (strain Rothamsted) (ABPV)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Replicase polyprotein contains helicase, VPg, protease and RNA-directed RNA polymerase functions.
    RNA-directed RNA polymerase replicates genomic and antigenomic RNA and transcribes the vial genome.
    The protease generates mature viral proteins from the precursor polyprotein.
    VPg is covalently linked to the 5'-end of genomic RNA.

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi544 – 5518ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type peptidase activity Source: UniProtKB-KW
    3. RNA binding Source: InterPro
    4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    5. RNA helicase activity Source: InterPro

    GO - Biological processi

    1. transcription, DNA-templated Source: InterPro
    2. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

    Keywords - Biological processi

    Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicase polyprotein (EC:2.7.7.48, EC:3.4.22.-)
    Gene namesi
    ORF Names:ORF1
    OrganismiAcute bee paralysis virus (strain Rothamsted) (ABPV)
    Taxonomic identifieri1217067 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesDicistroviridaeAparavirus
    Virus hostiApis mellifera (Honeybee) [TaxID: 7460]
    ProteomesiUP000006040: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 19061906Replicase polyproteinPRO_0000423158Add
    BLAST

    Post-translational modificationi

    Specific enzymatic cleavages in vivo by the viral protease yield a variety of precursors and mature proteins.Curated

    Structurei

    3D structure databases

    ProteinModelPortaliQ9DSN9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini513 – 692180SF3 helicasePROSITE-ProRule annotationAdd
    BLAST
    Domaini1638 – 1772135RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1124 – 1350227ProteaseAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi154 – 1574Poly-Leu
    Compositional biasi337 – 3404Poly-Asn

    Sequence similaritiesi

    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
    Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR024387. Pept_C3G_Picornavir.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF12381. Peptidase_C3G. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9DSN9-1 [UniParc]FASTAAdd to Basket

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    MNFTKQPASL KYLSSMKLIT SQDQDFFNFG EVSREFILEQ AYVNGYDFHM     50
    LHQDMNCIGF VLSIFDEDAR DEYEYKDLNC EYHENLFDAV VDSEFDKIWP 100
    HLVLKYITYY PCSLNWRGMP TIPIVYVSKH FWYELYRTGF LNKLYHCGSW 150
    TDILLLLSGD VETNPGPVET YKDLCRRKNI RKRKSRIREE IKMQQHIDKI 200
    IGQENEEYKI INVNMQGIFS FNEEKEIIKS TAWKFNSTLD KTNSIIDNLI 250
    PQLEETLAGF RKTYSKCESK IFGTISVVDV CVDLISALLQ VSFAKPAMKI 300
    ASLAVEVFRL IKKYVSNINI NIDKIKELLS YGKVALNNNN PIIHVTMQSN 350
    SPILEVLLQP NIIVSAIFIA LSVVFHKKFT YKKLGIEAMI KRLGDLGRAA 400
    KGCSDLNVVL NQAITNHMLE HFGKNVLGLK QEDELKVLVE GYRNWCDEVR 450
    DLVGHKINSD GELDSKSIVE NIMKDVYEIQ RIENMYKKGL EISRNIAELK 500
    LPTKLTISFN THMRYLTEVF KSVDTSGAFG NKPRTQPIVI WLFGESGRGK 550
    SGMTWPLAID LNNSLLDNVD EMRNFSKNIY MRNVEQEFWD NYQGQNIVCX 600
    DDFGQMRDSS SNPNPEFMEL IRTANIAPYP LHMAHLEDKR KTKFTSKVII 650
    MTSNVFEQDV NSLTFPDAFR RRVDLCAEVK NKDEFTKMCW SKSAGKMVQR 700
    LDKGKVKKIT GDIHSTVPYI VDLIDPESGE VYKTGLEYEE FLDMCLEKTS 750
    QCRDDSAKLN DFLMDYAEKR ANRSREIDEV CARTMDEAFV DAYDDVIDVN 800
    MQIETVDEME LIEPNKLREM IEQCSNKIVY TYEGIAVKIT SLAFKLATLN 850
    YEEQWEQIKE MKYYVKVSSG VNYLKRVLSQ GMKVCEEWMK EMINYVKEHP 900
    WMTVSLILGT LIGILTVVGF WKWLCSGDKK KNPIKRHFIN TGNVLILPDR 950
    ELNTFWKNQE SLDLRDMYIN RVEEHIISLL KLQHKVVLVP KVTKYILTTV 1000
    ENHAKISDKI ILITRNRYLN YQGKFVELIC GEINQFFIDP ETLDTNVEAF 1050
    ASADLKTFVQ RKPIVIEGPE FVEAQTSGDQ ITLRKQTQKV IEAFASSDAI 1100
    TMARKTPKFV ESDDVVEVSM QMWKDQVAQK LITNRVLTNL YKICLVKENG 1150
    DMVPLLNGLF VRSNIMLAPG HLVGFLSDSD TIEIRNLFDV VFRVPWKDVK 1200
    KVDVVNAFGE SKEAVLLCFP KFVCQHTDLV KHFQDSESMS KFKRCEVTLP 1250
    VLRYSDKMNR FLATLIECDK VEAYDRPYTL NDSSKGQYIL RQGLEYTMPT 1300
    TNGDCGAPLV INETQVIRKI AGIHVAGDAR GKAYAESISQ KDLIRAFSKI 1350
    DVSMQIQLDL DQTLNFNQQQ XIIPPNAEFG PEDLDFCDLP SLKMIPVGRL 1400
    SEPLFEPGKT DIRPSLVYGK ISEIKTKPAI LRNVIVDGKI VNIKHKNLKK 1450
    CAMDTPYVSK EMTEEAFQLV KSVWLKGMRN ELKKVLTYEE AICGNDSSEF 1500
    ISAINRSSSP GFPWIRDRIK GTKGKQGWFG AEGEYILDED VFEAVKTRIQ 1550
    NAKNGVRTPV MWVDTLKDER RPIEKVDQLK TRVFSNGPMD FSITFRMYYL 1600
    GFIAHLMENR ITNEVSIGTN VYSQDWNKTV RKLKTMGPKV IAGDFSTFDG 1650
    SLNVCIMEKF ADLANEFYDD GSENALIRHV LLMDVYNSTH ICGDSVYMMT 1700
    HSQPSGNPAT TPLNCFINSM GLRMVFELCS KKYSALNGTK CYVMKDFSKH 1750
    VSIVSYGDDN VINFSDEVSE WFNMETITEA FEKLGFTYTD ELKGKNGEVP 1800
    KWRTIEDVQY LKRKFRYDSK RKVWEAPLCM DTILEMPNWC RGSLDIQEGT 1850
    KVNCENAIME LSMHEEYVFD KWSKVISKAY QKATGDCLDI STYNGYAQER 1900
    FLNYYL 1906
    Length:1,906
    Mass (Da):219,430
    Last modified:March 1, 2001 - v1
    Checksum:i5A48F79063235FE4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF150629 Genomic RNA. Translation: AAG13118.1.
    RefSeqiNP_066241.1. NC_002548.1.

    Genome annotation databases

    GeneIDi911837.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF150629 Genomic RNA. Translation: AAG13118.1 .
    RefSeqi NP_066241.1. NC_002548.1.

    3D structure databases

    ProteinModelPortali Q9DSN9.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 911837.

    Family and domain databases

    InterProi IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR024387. Pept_C3G_Picornavir.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF12381. Peptidase_C3G. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the complete genome sequence of acute bee paralysis virus shows that it belongs to the novel group of insect-infecting RNA viruses."
      Govan V.A., Leat N., Allsopp M., Davison S.
      Virology 277:457-463(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiPOLN_ABPVR
    AccessioniPrimary (citable) accession number: Q9DSN9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2013
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3