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Q9DSN9

- POLN_ABPVR

UniProt

Q9DSN9 - POLN_ABPVR

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Protein

Replicase polyprotein

Gene

ORF1

Organism
Acute bee paralysis virus (strain Rothamsted) (ABPV)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Replicase polyprotein contains helicase, VPg, protease and RNA-directed RNA polymerase functions.
RNA-directed RNA polymerase replicates genomic and antigenomic RNA and transcribes the vial genome.
The protease generates mature viral proteins from the precursor polyprotein.
VPg is covalently linked to the 5'-end of genomic RNA.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi544 – 5518ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type peptidase activity Source: UniProtKB-KW
  3. RNA binding Source: InterPro
  4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  5. RNA helicase activity Source: InterPro

GO - Biological processi

  1. transcription, DNA-templated Source: InterPro
  2. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein (EC:2.7.7.48, EC:3.4.22.-)
Gene namesi
ORF Names:ORF1
OrganismiAcute bee paralysis virus (strain Rothamsted) (ABPV)
Taxonomic identifieri1217067 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesDicistroviridaeAparavirus
Virus hostiApis mellifera (Honeybee) [TaxID: 7460]
ProteomesiUP000006040: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19061906Replicase polyproteinPRO_0000423158Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo by the viral protease yield a variety of precursors and mature proteins.Curated

Structurei

3D structure databases

ProteinModelPortaliQ9DSN9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini513 – 692180SF3 helicasePROSITE-ProRule annotationAdd
BLAST
Domaini1638 – 1772135RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1124 – 1350227ProteaseAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi154 – 1574Poly-Leu
Compositional biasi337 – 3404Poly-Asn

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR024387. Pept_C3G_Picornavir.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF12381. Peptidase_C3G. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DSN9 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNFTKQPASL KYLSSMKLIT SQDQDFFNFG EVSREFILEQ AYVNGYDFHM
60 70 80 90 100
LHQDMNCIGF VLSIFDEDAR DEYEYKDLNC EYHENLFDAV VDSEFDKIWP
110 120 130 140 150
HLVLKYITYY PCSLNWRGMP TIPIVYVSKH FWYELYRTGF LNKLYHCGSW
160 170 180 190 200
TDILLLLSGD VETNPGPVET YKDLCRRKNI RKRKSRIREE IKMQQHIDKI
210 220 230 240 250
IGQENEEYKI INVNMQGIFS FNEEKEIIKS TAWKFNSTLD KTNSIIDNLI
260 270 280 290 300
PQLEETLAGF RKTYSKCESK IFGTISVVDV CVDLISALLQ VSFAKPAMKI
310 320 330 340 350
ASLAVEVFRL IKKYVSNINI NIDKIKELLS YGKVALNNNN PIIHVTMQSN
360 370 380 390 400
SPILEVLLQP NIIVSAIFIA LSVVFHKKFT YKKLGIEAMI KRLGDLGRAA
410 420 430 440 450
KGCSDLNVVL NQAITNHMLE HFGKNVLGLK QEDELKVLVE GYRNWCDEVR
460 470 480 490 500
DLVGHKINSD GELDSKSIVE NIMKDVYEIQ RIENMYKKGL EISRNIAELK
510 520 530 540 550
LPTKLTISFN THMRYLTEVF KSVDTSGAFG NKPRTQPIVI WLFGESGRGK
560 570 580 590 600
SGMTWPLAID LNNSLLDNVD EMRNFSKNIY MRNVEQEFWD NYQGQNIVCX
610 620 630 640 650
DDFGQMRDSS SNPNPEFMEL IRTANIAPYP LHMAHLEDKR KTKFTSKVII
660 670 680 690 700
MTSNVFEQDV NSLTFPDAFR RRVDLCAEVK NKDEFTKMCW SKSAGKMVQR
710 720 730 740 750
LDKGKVKKIT GDIHSTVPYI VDLIDPESGE VYKTGLEYEE FLDMCLEKTS
760 770 780 790 800
QCRDDSAKLN DFLMDYAEKR ANRSREIDEV CARTMDEAFV DAYDDVIDVN
810 820 830 840 850
MQIETVDEME LIEPNKLREM IEQCSNKIVY TYEGIAVKIT SLAFKLATLN
860 870 880 890 900
YEEQWEQIKE MKYYVKVSSG VNYLKRVLSQ GMKVCEEWMK EMINYVKEHP
910 920 930 940 950
WMTVSLILGT LIGILTVVGF WKWLCSGDKK KNPIKRHFIN TGNVLILPDR
960 970 980 990 1000
ELNTFWKNQE SLDLRDMYIN RVEEHIISLL KLQHKVVLVP KVTKYILTTV
1010 1020 1030 1040 1050
ENHAKISDKI ILITRNRYLN YQGKFVELIC GEINQFFIDP ETLDTNVEAF
1060 1070 1080 1090 1100
ASADLKTFVQ RKPIVIEGPE FVEAQTSGDQ ITLRKQTQKV IEAFASSDAI
1110 1120 1130 1140 1150
TMARKTPKFV ESDDVVEVSM QMWKDQVAQK LITNRVLTNL YKICLVKENG
1160 1170 1180 1190 1200
DMVPLLNGLF VRSNIMLAPG HLVGFLSDSD TIEIRNLFDV VFRVPWKDVK
1210 1220 1230 1240 1250
KVDVVNAFGE SKEAVLLCFP KFVCQHTDLV KHFQDSESMS KFKRCEVTLP
1260 1270 1280 1290 1300
VLRYSDKMNR FLATLIECDK VEAYDRPYTL NDSSKGQYIL RQGLEYTMPT
1310 1320 1330 1340 1350
TNGDCGAPLV INETQVIRKI AGIHVAGDAR GKAYAESISQ KDLIRAFSKI
1360 1370 1380 1390 1400
DVSMQIQLDL DQTLNFNQQQ XIIPPNAEFG PEDLDFCDLP SLKMIPVGRL
1410 1420 1430 1440 1450
SEPLFEPGKT DIRPSLVYGK ISEIKTKPAI LRNVIVDGKI VNIKHKNLKK
1460 1470 1480 1490 1500
CAMDTPYVSK EMTEEAFQLV KSVWLKGMRN ELKKVLTYEE AICGNDSSEF
1510 1520 1530 1540 1550
ISAINRSSSP GFPWIRDRIK GTKGKQGWFG AEGEYILDED VFEAVKTRIQ
1560 1570 1580 1590 1600
NAKNGVRTPV MWVDTLKDER RPIEKVDQLK TRVFSNGPMD FSITFRMYYL
1610 1620 1630 1640 1650
GFIAHLMENR ITNEVSIGTN VYSQDWNKTV RKLKTMGPKV IAGDFSTFDG
1660 1670 1680 1690 1700
SLNVCIMEKF ADLANEFYDD GSENALIRHV LLMDVYNSTH ICGDSVYMMT
1710 1720 1730 1740 1750
HSQPSGNPAT TPLNCFINSM GLRMVFELCS KKYSALNGTK CYVMKDFSKH
1760 1770 1780 1790 1800
VSIVSYGDDN VINFSDEVSE WFNMETITEA FEKLGFTYTD ELKGKNGEVP
1810 1820 1830 1840 1850
KWRTIEDVQY LKRKFRYDSK RKVWEAPLCM DTILEMPNWC RGSLDIQEGT
1860 1870 1880 1890 1900
KVNCENAIME LSMHEEYVFD KWSKVISKAY QKATGDCLDI STYNGYAQER

FLNYYL
Length:1,906
Mass (Da):219,430
Last modified:March 1, 2001 - v1
Checksum:i5A48F79063235FE4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF150629 Genomic RNA. Translation: AAG13118.1.
RefSeqiNP_066241.1. NC_002548.1.

Genome annotation databases

GeneIDi911837.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF150629 Genomic RNA. Translation: AAG13118.1 .
RefSeqi NP_066241.1. NC_002548.1.

3D structure databases

ProteinModelPortali Q9DSN9.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 911837.

Family and domain databases

InterProi IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR024387. Pept_C3G_Picornavir.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF12381. Peptidase_C3G. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis of the complete genome sequence of acute bee paralysis virus shows that it belongs to the novel group of insect-infecting RNA viruses."
    Govan V.A., Leat N., Allsopp M., Davison S.
    Virology 277:457-463(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiPOLN_ABPVR
AccessioniPrimary (citable) accession number: Q9DSN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: March 1, 2001
Last modified: October 1, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3