Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9DSN9 (POLN_ABPVR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicase polyprotein

EC=2.7.7.48
EC=3.4.22.-
Gene names
ORF Names:ORF1
OrganismAcute bee paralysis virus (strain Rothamsted) (ABPV) [Reference proteome]
Taxonomic identifier1217067 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesDicistroviridaeAparavirus
Virus hostApis mellifera (Honeybee) [TaxID: 7460]

Protein attributes

Sequence length1906 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Replicase polyprotein contains helicase, VPg, protease and RNA-directed RNA polymerase functions.

RNA-directed RNA polymerase replicates genomic and antigenomic RNA and transcribes the vial genome.

The protease generates mature viral proteins from the precursor polyprotein.

VPg is covalently linked to the 5'-end of genomic RNA.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Post-translational modification

Specific enzymatic cleavages in vivo by the viral protease yield a variety of precursors and mature proteins Probable.

Sequence similarities

Contains 1 RdRp catalytic domain.

Contains 1 SF3 helicase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19061906Replicase polyprotein
PRO_0000423158

Regions

Domain513 – 692180SF3 helicase
Domain1638 – 1772135RdRp catalytic
Nucleotide binding544 – 5518ATP Potential
Region1124 – 1350227Protease
Compositional bias154 – 1574Poly-Leu
Compositional bias337 – 3404Poly-Asn

Sequences

Sequence LengthMass (Da)Tools
Q9DSN9 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 5A48F79063235FE4

FASTA1,906219,430
        10         20         30         40         50         60 
MNFTKQPASL KYLSSMKLIT SQDQDFFNFG EVSREFILEQ AYVNGYDFHM LHQDMNCIGF 

        70         80         90        100        110        120 
VLSIFDEDAR DEYEYKDLNC EYHENLFDAV VDSEFDKIWP HLVLKYITYY PCSLNWRGMP 

       130        140        150        160        170        180 
TIPIVYVSKH FWYELYRTGF LNKLYHCGSW TDILLLLSGD VETNPGPVET YKDLCRRKNI 

       190        200        210        220        230        240 
RKRKSRIREE IKMQQHIDKI IGQENEEYKI INVNMQGIFS FNEEKEIIKS TAWKFNSTLD 

       250        260        270        280        290        300 
KTNSIIDNLI PQLEETLAGF RKTYSKCESK IFGTISVVDV CVDLISALLQ VSFAKPAMKI 

       310        320        330        340        350        360 
ASLAVEVFRL IKKYVSNINI NIDKIKELLS YGKVALNNNN PIIHVTMQSN SPILEVLLQP 

       370        380        390        400        410        420 
NIIVSAIFIA LSVVFHKKFT YKKLGIEAMI KRLGDLGRAA KGCSDLNVVL NQAITNHMLE 

       430        440        450        460        470        480 
HFGKNVLGLK QEDELKVLVE GYRNWCDEVR DLVGHKINSD GELDSKSIVE NIMKDVYEIQ 

       490        500        510        520        530        540 
RIENMYKKGL EISRNIAELK LPTKLTISFN THMRYLTEVF KSVDTSGAFG NKPRTQPIVI 

       550        560        570        580        590        600 
WLFGESGRGK SGMTWPLAID LNNSLLDNVD EMRNFSKNIY MRNVEQEFWD NYQGQNIVCX 

       610        620        630        640        650        660 
DDFGQMRDSS SNPNPEFMEL IRTANIAPYP LHMAHLEDKR KTKFTSKVII MTSNVFEQDV 

       670        680        690        700        710        720 
NSLTFPDAFR RRVDLCAEVK NKDEFTKMCW SKSAGKMVQR LDKGKVKKIT GDIHSTVPYI 

       730        740        750        760        770        780 
VDLIDPESGE VYKTGLEYEE FLDMCLEKTS QCRDDSAKLN DFLMDYAEKR ANRSREIDEV 

       790        800        810        820        830        840 
CARTMDEAFV DAYDDVIDVN MQIETVDEME LIEPNKLREM IEQCSNKIVY TYEGIAVKIT 

       850        860        870        880        890        900 
SLAFKLATLN YEEQWEQIKE MKYYVKVSSG VNYLKRVLSQ GMKVCEEWMK EMINYVKEHP 

       910        920        930        940        950        960 
WMTVSLILGT LIGILTVVGF WKWLCSGDKK KNPIKRHFIN TGNVLILPDR ELNTFWKNQE 

       970        980        990       1000       1010       1020 
SLDLRDMYIN RVEEHIISLL KLQHKVVLVP KVTKYILTTV ENHAKISDKI ILITRNRYLN 

      1030       1040       1050       1060       1070       1080 
YQGKFVELIC GEINQFFIDP ETLDTNVEAF ASADLKTFVQ RKPIVIEGPE FVEAQTSGDQ 

      1090       1100       1110       1120       1130       1140 
ITLRKQTQKV IEAFASSDAI TMARKTPKFV ESDDVVEVSM QMWKDQVAQK LITNRVLTNL 

      1150       1160       1170       1180       1190       1200 
YKICLVKENG DMVPLLNGLF VRSNIMLAPG HLVGFLSDSD TIEIRNLFDV VFRVPWKDVK 

      1210       1220       1230       1240       1250       1260 
KVDVVNAFGE SKEAVLLCFP KFVCQHTDLV KHFQDSESMS KFKRCEVTLP VLRYSDKMNR 

      1270       1280       1290       1300       1310       1320 
FLATLIECDK VEAYDRPYTL NDSSKGQYIL RQGLEYTMPT TNGDCGAPLV INETQVIRKI 

      1330       1340       1350       1360       1370       1380 
AGIHVAGDAR GKAYAESISQ KDLIRAFSKI DVSMQIQLDL DQTLNFNQQQ XIIPPNAEFG 

      1390       1400       1410       1420       1430       1440 
PEDLDFCDLP SLKMIPVGRL SEPLFEPGKT DIRPSLVYGK ISEIKTKPAI LRNVIVDGKI 

      1450       1460       1470       1480       1490       1500 
VNIKHKNLKK CAMDTPYVSK EMTEEAFQLV KSVWLKGMRN ELKKVLTYEE AICGNDSSEF 

      1510       1520       1530       1540       1550       1560 
ISAINRSSSP GFPWIRDRIK GTKGKQGWFG AEGEYILDED VFEAVKTRIQ NAKNGVRTPV 

      1570       1580       1590       1600       1610       1620 
MWVDTLKDER RPIEKVDQLK TRVFSNGPMD FSITFRMYYL GFIAHLMENR ITNEVSIGTN 

      1630       1640       1650       1660       1670       1680 
VYSQDWNKTV RKLKTMGPKV IAGDFSTFDG SLNVCIMEKF ADLANEFYDD GSENALIRHV 

      1690       1700       1710       1720       1730       1740 
LLMDVYNSTH ICGDSVYMMT HSQPSGNPAT TPLNCFINSM GLRMVFELCS KKYSALNGTK 

      1750       1760       1770       1780       1790       1800 
CYVMKDFSKH VSIVSYGDDN VINFSDEVSE WFNMETITEA FEKLGFTYTD ELKGKNGEVP 

      1810       1820       1830       1840       1850       1860 
KWRTIEDVQY LKRKFRYDSK RKVWEAPLCM DTILEMPNWC RGSLDIQEGT KVNCENAIME 

      1870       1880       1890       1900 
LSMHEEYVFD KWSKVISKAY QKATGDCLDI STYNGYAQER FLNYYL 

« Hide

References

[1]"Analysis of the complete genome sequence of acute bee paralysis virus shows that it belongs to the novel group of insect-infecting RNA viruses."
Govan V.A., Leat N., Allsopp M., Davison S.
Virology 277:457-463(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF150629 Genomic RNA. Translation: AAG13118.1.
RefSeqNP_066241.1. NC_002548.1.

3D structure databases

ProteinModelPortalQ9DSN9.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID911837.

Family and domain databases

InterProIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR024387. Pept_C3G_Picornavir.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF12381. Peptidase_C3G. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePOLN_ABPVR
AccessionPrimary (citable) accession number: Q9DSN9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families