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Q9DR81

- HEMA_CVBON

UniProt

Q9DR81 - HEMA_CVBON

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Protein
Hemagglutinin-esterase
Gene
HE, 2b
Organism
Bovine coronavirus (strain Ontario) (BCoV) (BCV)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system By similarity.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401Nucleophile By similarity
Active sitei228 – 2281Charge relay system By similarity
Active sitei329 – 3291Charge relay system By similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2b
OrganismiBovine coronavirus (strain Ontario) (BCoV) (BCV)
Taxonomic identifieri231422 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]

Subcellular locationi

Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host cell membrane; Single-pass type I membrane protein Reviewed prediction
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 392374Virion surface Reviewed prediction
Add
BLAST
Transmembranei393 – 41321Helical; Reviewed prediction
Add
BLAST
Topological domaini414 – 42411Intravirion Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 By similarity
Add
BLAST
Chaini19 – 424406Hemagglutinin-esterase
PRO_0000037141Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 65 By similarity
Glycosylationi54 – 541N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi89 – 891N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi113 ↔ 162 By similarity
Glycosylationi153 – 1531N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi197 ↔ 276 By similarity
Disulfide bondi205 ↔ 249 By similarity
Glycosylationi236 – 2361N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi301 – 3011N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi307 ↔ 312 By similarity
Glycosylationi316 – 3161N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi347 ↔ 371 By similarity
Glycosylationi358 – 3581N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

N-glycosylated in the RER.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

Structurei

3D structure databases

ProteinModelPortaliQ9DR81.
SMRiQ9DR81. Positions 19-376.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni7 – 127121Esterase domain first part By similarity
Add
BLAST
Regioni128 – 266139Receptor binding By similarity
Add
BLAST
Regioni267 – 379113Esterase domain second part By similarity
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DR81-1 [UniParc]FASTAAdd to Basket

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MFLLPRFVLV SCIIGSLGFD NPPTNVVSHL NGDWFLFGDS RSDCNHVVTT    50
NPRNYSYMDL NPALCGSGKI SSKAGNSIFR SFHFTDFYNY TGEGQQIIFY 100
EGVNFTPYHA FKCTTSGSND IWMQNKGLFY TQVYKNMAVY RSLTFVNVPY 150
VYNGSAQSTA LCKSGSLVLN NPAYIAREAN FGDYYYKVEA DFYLSGCDEY 200
IVPLCIFNGK FLSNTKYYDD SQYYFNKDTG VIYGLNSTET ITTGFDFNCH 250
YLVLPSGNYL AISNELLLTV PTKAICLNKR KDFTPVQVVD SRWNNARQSD 300
NMTAVACQPP YCYFRNSTTN YVGVYDINHG DAGFTSILSG LLYDSPCFSQ 350
QGVFRYDNVS SVWPLYPYGR CPTAADINTP DVPICVYDPL PIIFLGILLG 400
VAVIIIVVLL LYFMVDNGTR LHDA 424
Length:424
Mass (Da):47,652
Last modified:March 1, 2001 - v1
Checksum:i999752B9FCA231BA
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti394 – 3941F → L in strain: Isolate BCO.43277.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF239308 Genomic RNA. Translation: AAG60544.1.
AF239309 Genomic RNA. Translation: AAG40622.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF239308 Genomic RNA. Translation: AAG60544.1 .
AF239309 Genomic RNA. Translation: AAG40622.1 .

3D structure databases

ProteinModelPortali Q9DR81.
SMRi Q9DR81. Positions 19-376.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Bovine coronaviruses associated with enteric and respiratory diseases in Canadian dairy cattle display different reactivities to anti-HE monoclonal antibodies and distinct amino acid changes in their HE, S and ns4.9 protein."
    Gelinas A.-M., Boutin M., Sasseville A.M.-J., Dea S.
    Virus Res. 76:43-57(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate BCO.43277 and Isolate BCO.44175.
  2. "Identification of specific variations within the HE, S1, and ORF4 genes of bovine coronaviruses associated with enteric and respiratory diseases in dairy cattle."
    Gelinas A.-M., Sasseville A.M.-J., Dea S.
    Adv. Exp. Med. Biol. 494:63-67(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Isolate BCO.43277 and Isolate BCO.44175.

Entry informationi

Entry nameiHEMA_CVBON
AccessioniPrimary (citable) accession number: Q9DR81
Secondary accession number(s): Q98VL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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