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Q9DGE2

- MK14A_DANRE

UniProt

Q9DGE2 - MK14A_DANRE

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Protein
Mitogen-activated protein kinase 14A
Gene
mapk14a, mapk14
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Mapk14a is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. Required for cytokinesis on the future dorsal side of the blastodisc, suggesting a role in symmetrical and synchronous blastomere cleavage.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Magnesium By similarity.By similarity

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation by the dual specificity kinase, MKK3.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATP By similarityBy similarity
Active sitei169 – 1691Proton acceptor By similarityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 399ATP By similarityBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. embryonic cleavage Source: UniProtKB
  2. intracellular signal transduction Source: UniProtKB
  3. p38MAPK cascade Source: UniProtKB
  4. positive regulation of myoblast differentiation Source: UniProtKB
  5. positive regulation of myoblast fusion Source: UniProtKB
  6. positive regulation of myotube differentiation Source: UniProtKB
  7. regulation of protein stability Source: ZFIN
  8. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  9. response to stress Source: UniProtKB
  10. somitogenesis Source: ZFIN
  11. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_180916. Oxidative Stress Induced Senescence.
REACT_206122. ADP signalling through P2Y purinoceptor 1.
REACT_207781. KSRP destabilizes mRNA.
REACT_211279. Platelet sensitization by LDL.
REACT_215805. activated TAK1 mediates p38 MAPK activation.
REACT_224357. Activation of PPARGC1A (PGC-1alpha) by phosphorylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 14A (EC:2.7.11.24)
Short name:
MAP kinase 14A
Short name:
MAPK 14A
Alternative name(s):
Mitogen-activated protein kinase p38a
Short name:
MAP kinase p38a
Short name:
zp38a
Gene namesi
Name:mapk14a
Synonyms:mapk14
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 8

Organism-specific databases

ZFINiZDB-GENE-010202-2. mapk14a.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi181 – 1811T → A: Loss of enzyme activation. 1 Publication
Mutagenesisi183 – 1831Y → F: Loss of enzyme activation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361Mitogen-activated protein kinase 14A
PRO_0000186295Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811Phosphothreonine; by MAP2K31 Publication
Modified residuei183 – 1831Phosphotyrosine; by MAP2K31 Publication

Post-translational modificationi

Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9DGE2.

Expressioni

Developmental stagei

Expressed as the predominant form of mapk14 both maternally and zygotically throughout development.1 Publication

Gene expression databases

BgeeiQ9DGE2.

Structurei

3D structure databases

ProteinModelPortaliQ9DGE2.
SMRiQ9DGE2. Positions 5-352.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 309285Protein kinase
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi181 – 1833TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074271.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
KOiK04441.
OMAiMEEQFNG.
OrthoDBiEOG7PCJGV.
PhylomeDBiQ9DGE2.
TreeFamiTF105100.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DGE2-1 [UniParc]FASTAAdd to Basket

« Hide

MSQKERPTFY RQEVNKTIWE VPVQYQNLSP VGSGAYGSVC SAFDAKTGFK    50
VAVKKLSRPF QSIIHAKRTY RELRLLKHMR HENVIGLLDV FTPATSLKEF 100
NDVYLVTHLM GADLNNIVKC QKLTDDHVQF LIYQILRGLK YIHSADIIHR 150
DLKPSNLAVN EDCELKILDF GLARHTDDEM TGYVATRWYR APEIMLNWMH 200
YNVTVDIWSV GCIMAELLTG RTLFPGTDHI NQLQQIMRLT GTPPSSLISR 250
MPSHEARTYI SSLPQMPKRN FADVFIGANP QAVDLLEKML VLDTDKRITA 300
AEALAHPYFA QYHDPDDEPE AEPFDQSFES RELDIEEWKR QTYEEMISFE 350
PPVFDVDEME S 361
Length:361
Mass (Da):41,633
Last modified:March 1, 2001 - v1
Checksum:iB3FD9577831718C0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB030897 mRNA. Translation: BAB11807.1.
BC044128 mRNA. Translation: AAH44128.2.
RefSeqiNP_571797.1. NM_131722.1.
UniGeneiDr.72252.

Genome annotation databases

EnsembliENSDART00000040362; ENSDARP00000040361; ENSDARG00000000857.
GeneIDi65237.
KEGGidre:65237.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB030897 mRNA. Translation: BAB11807.1 .
BC044128 mRNA. Translation: AAH44128.2 .
RefSeqi NP_571797.1. NM_131722.1.
UniGenei Dr.72252.

3D structure databases

ProteinModelPortali Q9DGE2.
SMRi Q9DGE2. Positions 5-352.
ModBasei Search...

Proteomic databases

PRIDEi Q9DGE2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000040362 ; ENSDARP00000040361 ; ENSDARG00000000857 .
GeneIDi 65237.
KEGGi dre:65237.

Organism-specific databases

CTDi 65237.
ZFINi ZDB-GENE-010202-2. mapk14a.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074271.
HOGENOMi HOG000233024.
HOVERGENi HBG014652.
KOi K04441.
OMAi MEEQFNG.
OrthoDBi EOG7PCJGV.
PhylomeDBi Q9DGE2.
TreeFami TF105100.

Enzyme and pathway databases

Reactomei REACT_180916. Oxidative Stress Induced Senescence.
REACT_206122. ADP signalling through P2Y purinoceptor 1.
REACT_207781. KSRP destabilizes mRNA.
REACT_211279. Platelet sensitization by LDL.
REACT_215805. activated TAK1 mediates p38 MAPK activation.
REACT_224357. Activation of PPARGC1A (PGC-1alpha) by phosphorylation.

Miscellaneous databases

NextBioi 20902034.
PROi Q9DGE2.

Gene expression databases

Bgeei Q9DGE2.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01773. P38MAPKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Asymmetric p38 activation in zebrafish: its possible role in symmetric and synchronous cleavage."
    Fujii R., Yamashita S., Hibi M., Hirano T.
    J. Cell Biol. 150:1335-1348(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, PHOSPHORYLATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF THR-181 AND TYR-183.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: AB.

Entry informationi

Entry nameiMK14A_DANRE
AccessioniPrimary (citable) accession number: Q9DGE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2001
Last modified: September 3, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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