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Q9DGE2 (MK14A_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 14A

Short name=MAP kinase 14A
Short name=MAPK 14A
EC=2.7.11.24
Alternative name(s):
Mitogen-activated protein kinase p38a
Short name=MAP kinase p38a
Short name=zp38a
Gene names
Name:mapk14a
Synonyms:mapk14
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Mapk14a is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. Required for cytokinesis on the future dorsal side of the blastodisc, suggesting a role in symmetrical and synchronous blastomere cleavage. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. UniProtKB Q90336

Cofactor

Magnesium By similarity. UniProtKB Q90336

Enzyme regulation

Activated by threonine and tyrosine phosphorylation by the dual specificity kinase, MKK3. Ref.1

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Developmental stage

Expressed as the predominant form of mapk14 both maternally and zygotically throughout development. Ref.1

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processStress response
Transcription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   LigandATP-binding
Nucleotide-binding
   Molecular functionDevelopmental protein
Kinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processembryonic cleavage

Inferred from direct assay Ref.1. Source: UniProtKB

intracellular signal transduction

Inferred from direct assay Ref.1. Source: UniProtKB

p38MAPK cascade

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of myoblast differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of myoblast fusion

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of myotube differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of protein stability

Inferred from mutant phenotype PubMed 21159776. Source: ZFIN

regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: UniProtKB

response to stress

Inferred from direct assay Ref.1. Source: UniProtKB

somitogenesis

Inferred from mutant phenotype PubMed 21159776. Source: ZFIN

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 361361Mitogen-activated protein kinase 14A
PRO_0000186295

Regions

Domain25 – 309285Protein kinase
Nucleotide binding31 – 399ATP By similarity UniProtKB Q16539
Motif181 – 1833TXY

Sites

Active site1691Proton acceptor By similarity UniProtKB Q16539
Binding site541ATP By similarity UniProtKB Q16539

Amino acid modifications

Modified residue1811Phosphothreonine; by MAP2K3 Ref.1
Modified residue1831Phosphotyrosine; by MAP2K3 Ref.1

Experimental info

Mutagenesis1811T → A: Loss of enzyme activation. Ref.1
Mutagenesis1831Y → F: Loss of enzyme activation. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9DGE2 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: B3FD9577831718C0

FASTA36141,633
        10         20         30         40         50         60 
MSQKERPTFY RQEVNKTIWE VPVQYQNLSP VGSGAYGSVC SAFDAKTGFK VAVKKLSRPF 

        70         80         90        100        110        120 
QSIIHAKRTY RELRLLKHMR HENVIGLLDV FTPATSLKEF NDVYLVTHLM GADLNNIVKC 

       130        140        150        160        170        180 
QKLTDDHVQF LIYQILRGLK YIHSADIIHR DLKPSNLAVN EDCELKILDF GLARHTDDEM 

       190        200        210        220        230        240 
TGYVATRWYR APEIMLNWMH YNVTVDIWSV GCIMAELLTG RTLFPGTDHI NQLQQIMRLT 

       250        260        270        280        290        300 
GTPPSSLISR MPSHEARTYI SSLPQMPKRN FADVFIGANP QAVDLLEKML VLDTDKRITA 

       310        320        330        340        350        360 
AEALAHPYFA QYHDPDDEPE AEPFDQSFES RELDIEEWKR QTYEEMISFE PPVFDVDEME 


S 

« Hide

References

« Hide 'large scale' references
[1]"Asymmetric p38 activation in zebrafish: its possible role in symmetric and synchronous cleavage."
Fujii R., Yamashita S., Hibi M., Hirano T.
J. Cell Biol. 150:1335-1348(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, PHOSPHORYLATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF THR-181 AND TYR-183.
[2]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: AB.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB030897 mRNA. Translation: BAB11807.1.
BC044128 mRNA. Translation: AAH44128.2.
RefSeqNP_571797.1. NM_131722.1.
UniGeneDr.72252.

3D structure databases

ProteinModelPortalQ9DGE2.
SMRQ9DGE2. Positions 5-352.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9DGE2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000040362; ENSDARP00000040361; ENSDARG00000000857.
GeneID65237.
KEGGdre:65237.

Organism-specific databases

CTD65237.
ZFINZDB-GENE-010202-2. mapk14a.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000074271.
HOGENOMHOG000233024.
HOVERGENHBG014652.
KOK04441.
OMAMEEQFNG.
OrthoDBEOG7PCJGV.
PhylomeDBQ9DGE2.
TreeFamTF105100.

Gene expression databases

BgeeQ9DGE2.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01773. P38MAPKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20902034.
PROQ9DGE2.

Entry information

Entry nameMK14A_DANRE
AccessionPrimary (citable) accession number: Q9DGE2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families