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Q9DGE2

- MK14A_DANRE

UniProt

Q9DGE2 - MK14A_DANRE

Protein

Mitogen-activated protein kinase 14A

Gene

mapk14a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Mapk14a is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. Required for cytokinesis on the future dorsal side of the blastodisc, suggesting a role in symmetrical and synchronous blastomere cleavage.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.By similarity

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation by the dual specificity kinase, MKK3.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei54 – 541ATPPROSITE-ProRule annotation
    Active sitei169 – 1691Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB

    GO - Biological processi

    1. embryonic cleavage Source: UniProtKB
    2. intracellular signal transduction Source: UniProtKB
    3. p38MAPK cascade Source: UniProtKB
    4. positive regulation of myoblast differentiation Source: UniProtKB
    5. positive regulation of myoblast fusion Source: UniProtKB
    6. positive regulation of myotube differentiation Source: UniProtKB
    7. regulation of protein stability Source: ZFIN
    8. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    9. response to stress Source: UniProtKB
    10. somitogenesis Source: ZFIN
    11. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Stress response, Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_180916. Oxidative Stress Induced Senescence.
    REACT_206122. ADP signalling through P2Y purinoceptor 1.
    REACT_207781. KSRP destabilizes mRNA.
    REACT_211279. Platelet sensitization by LDL.
    REACT_215805. activated TAK1 mediates p38 MAPK activation.
    REACT_224357. Activation of PPARGC1A (PGC-1alpha) by phosphorylation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 14A (EC:2.7.11.24)
    Short name:
    MAP kinase 14A
    Short name:
    MAPK 14A
    Alternative name(s):
    Mitogen-activated protein kinase p38a
    Short name:
    MAP kinase p38a
    Short name:
    zp38a
    Gene namesi
    Name:mapk14a
    Synonyms:mapk14
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 8

    Organism-specific databases

    ZFINiZDB-GENE-010202-2. mapk14a.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi181 – 1811T → A: Loss of enzyme activation. 1 Publication
    Mutagenesisi183 – 1831Y → F: Loss of enzyme activation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 361361Mitogen-activated protein kinase 14APRO_0000186295Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei181 – 1811Phosphothreonine; by MAP2K31 Publication
    Modified residuei183 – 1831Phosphotyrosine; by MAP2K31 Publication

    Post-translational modificationi

    Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ9DGE2.

    Expressioni

    Developmental stagei

    Expressed as the predominant form of mapk14 both maternally and zygotically throughout development.1 Publication

    Gene expression databases

    BgeeiQ9DGE2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9DGE2.
    SMRiQ9DGE2. Positions 5-352.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 309285Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi181 – 1833TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000074271.
    HOGENOMiHOG000233024.
    HOVERGENiHBG014652.
    KOiK04441.
    OMAiMEEQFNG.
    OrthoDBiEOG7PCJGV.
    PhylomeDBiQ9DGE2.
    TreeFamiTF105100.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008352. MAPK_p38.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01773. P38MAPKINASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9DGE2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQKERPTFY RQEVNKTIWE VPVQYQNLSP VGSGAYGSVC SAFDAKTGFK    50
    VAVKKLSRPF QSIIHAKRTY RELRLLKHMR HENVIGLLDV FTPATSLKEF 100
    NDVYLVTHLM GADLNNIVKC QKLTDDHVQF LIYQILRGLK YIHSADIIHR 150
    DLKPSNLAVN EDCELKILDF GLARHTDDEM TGYVATRWYR APEIMLNWMH 200
    YNVTVDIWSV GCIMAELLTG RTLFPGTDHI NQLQQIMRLT GTPPSSLISR 250
    MPSHEARTYI SSLPQMPKRN FADVFIGANP QAVDLLEKML VLDTDKRITA 300
    AEALAHPYFA QYHDPDDEPE AEPFDQSFES RELDIEEWKR QTYEEMISFE 350
    PPVFDVDEME S 361
    Length:361
    Mass (Da):41,633
    Last modified:March 1, 2001 - v1
    Checksum:iB3FD9577831718C0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB030897 mRNA. Translation: BAB11807.1.
    BC044128 mRNA. Translation: AAH44128.2.
    RefSeqiNP_571797.1. NM_131722.1.
    UniGeneiDr.72252.

    Genome annotation databases

    EnsembliENSDART00000040362; ENSDARP00000040361; ENSDARG00000000857.
    GeneIDi65237.
    KEGGidre:65237.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB030897 mRNA. Translation: BAB11807.1 .
    BC044128 mRNA. Translation: AAH44128.2 .
    RefSeqi NP_571797.1. NM_131722.1.
    UniGenei Dr.72252.

    3D structure databases

    ProteinModelPortali Q9DGE2.
    SMRi Q9DGE2. Positions 5-352.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9DGE2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000040362 ; ENSDARP00000040361 ; ENSDARG00000000857 .
    GeneIDi 65237.
    KEGGi dre:65237.

    Organism-specific databases

    CTDi 65237.
    ZFINi ZDB-GENE-010202-2. mapk14a.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000074271.
    HOGENOMi HOG000233024.
    HOVERGENi HBG014652.
    KOi K04441.
    OMAi MEEQFNG.
    OrthoDBi EOG7PCJGV.
    PhylomeDBi Q9DGE2.
    TreeFami TF105100.

    Enzyme and pathway databases

    Reactomei REACT_180916. Oxidative Stress Induced Senescence.
    REACT_206122. ADP signalling through P2Y purinoceptor 1.
    REACT_207781. KSRP destabilizes mRNA.
    REACT_211279. Platelet sensitization by LDL.
    REACT_215805. activated TAK1 mediates p38 MAPK activation.
    REACT_224357. Activation of PPARGC1A (PGC-1alpha) by phosphorylation.

    Miscellaneous databases

    NextBioi 20902034.
    PROi Q9DGE2.

    Gene expression databases

    Bgeei Q9DGE2.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008352. MAPK_p38.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01773. P38MAPKINASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Asymmetric p38 activation in zebrafish: its possible role in symmetric and synchronous cleavage."
      Fujii R., Yamashita S., Hibi M., Hirano T.
      J. Cell Biol. 150:1335-1348(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, PHOSPHORYLATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF THR-181 AND TYR-183.
    2. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: AB.

    Entry informationi

    Entry nameiMK14A_DANRE
    AccessioniPrimary (citable) accession number: Q9DGE2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3