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Protein

Mitogen-activated protein kinase 14A

Gene

mapk14a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Mapk14a is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. Required for cytokinesis on the future dorsal side of the blastodisc, suggesting a role in symmetrical and synchronous blastomere cleavage.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation by the dual specificity kinase, MKK3.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1
Active sitei169Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 39ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: UniProtKB

GO - Biological processi

  • cellular response to UV Source: UniProtKB
  • embryonic cleavage Source: UniProtKB
  • p38MAPK cascade Source: UniProtKB
  • positive regulation of myoblast differentiation Source: UniProtKB
  • positive regulation of myoblast fusion Source: UniProtKB
  • positive regulation of myotube differentiation Source: UniProtKB
  • regulation of collateral sprouting in absence of injury Source: ZFIN
  • regulation of gene expression Source: GO_Central
  • regulation of protein stability Source: ZFIN
  • regulation of transcription by RNA polymerase II Source: UniProtKB
  • somitogenesis Source: ZFIN
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processStress response, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24 928
ReactomeiR-DRE-168638 NOD1/2 Signaling Pathway
R-DRE-171007 p38MAPK events
R-DRE-198753 ERK/MAPK targets
R-DRE-2559580 Oxidative Stress Induced Senescence
R-DRE-375170 CDO in myogenesis
R-DRE-418592 ADP signalling through P2Y purinoceptor 1
R-DRE-432142 Platelet sensitization by LDL
R-DRE-4420097 VEGFA-VEGFR2 Pathway
R-DRE-450302 activated TAK1 mediates p38 MAPK activation
R-DRE-450341 Activation of the AP-1 family of transcription factors
R-DRE-6798695 Neutrophil degranulation
R-DRE-6804756 Regulation of TP53 Activity through Phosphorylation

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 14A (EC:2.7.11.24)
Short name:
MAP kinase 14A
Short name:
MAPK 14A
Alternative name(s):
Mitogen-activated protein kinase p38a
Short name:
MAP kinase p38a
Short name:
zp38a
Gene namesi
Name:mapk14a
Synonyms:mapk14
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 8

Organism-specific databases

ZFINiZDB-GENE-010202-2 mapk14a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi181T → A: Loss of enzyme activation. 1 Publication1
Mutagenesisi183Y → F: Loss of enzyme activation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001862951 – 361Mitogen-activated protein kinase 14AAdd BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181Phosphothreonine; by MAP2K31 Publication1
Modified residuei183Phosphotyrosine; by MAP2K31 Publication1

Post-translational modificationi

Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9DGE2
PRIDEiQ9DGE2

PTM databases

iPTMnetiQ9DGE2

Expressioni

Developmental stagei

Expressed as the predominant form of mapk14 both maternally and zygotically throughout development.1 Publication

Gene expression databases

BgeeiENSDARG00000000857
ExpressionAtlasiQ9DGE2 baseline and differential

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000040361

Structurei

3D structure databases

ProteinModelPortaliQ9DGE2
SMRiQ9DGE2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 309Protein kinasePROSITE-ProRule annotationAdd BLAST285

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi181 – 183TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
GeneTreeiENSGT00550000074271
HOGENOMiHOG000233024
HOVERGENiHBG014652
InParanoidiQ9DGE2
KOiK04441
OMAiDENMAEP
OrthoDBiEOG091G08QL
PhylomeDBiQ9DGE2
TreeFamiTF105100

Family and domain databases

CDDicd07877 STKc_p38alpha, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR008352 MAPK_p38-like
IPR038784 p38alpha
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR01773 P38MAPKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

Q9DGE2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQKERPTFY RQEVNKTIWE VPVQYQNLSP VGSGAYGSVC SAFDAKTGFK
60 70 80 90 100
VAVKKLSRPF QSIIHAKRTY RELRLLKHMR HENVIGLLDV FTPATSLKEF
110 120 130 140 150
NDVYLVTHLM GADLNNIVKC QKLTDDHVQF LIYQILRGLK YIHSADIIHR
160 170 180 190 200
DLKPSNLAVN EDCELKILDF GLARHTDDEM TGYVATRWYR APEIMLNWMH
210 220 230 240 250
YNVTVDIWSV GCIMAELLTG RTLFPGTDHI NQLQQIMRLT GTPPSSLISR
260 270 280 290 300
MPSHEARTYI SSLPQMPKRN FADVFIGANP QAVDLLEKML VLDTDKRITA
310 320 330 340 350
AEALAHPYFA QYHDPDDEPE AEPFDQSFES RELDIEEWKR QTYEEMISFE
360
PPVFDVDEME S
Length:361
Mass (Da):41,633
Last modified:March 1, 2001 - v1
Checksum:iB3FD9577831718C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030897 mRNA Translation: BAB11807.1
BC044128 mRNA Translation: AAH44128.2
RefSeqiNP_571797.1, NM_131722.1
UniGeneiDr.72252

Genome annotation databases

EnsembliENSDART00000040362; ENSDARP00000040361; ENSDARG00000000857
GeneIDi65237
KEGGidre:65237

Similar proteinsi

Entry informationi

Entry nameiMK14A_DANRE
AccessioniPrimary (citable) accession number: Q9DGE2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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