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Q9DGE1

- MK14B_DANRE

UniProt

Q9DGE1 - MK14B_DANRE

Protein

Mitogen-activated protein kinase 14B

Gene

mapk14b

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Mapk14b is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.By similarity

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation by the dual specificity kinase, MKK3.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei54 – 541ATPPROSITE-ProRule annotation
    Active sitei169 – 1691Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB

    GO - Biological processi

    1. intracellular signal transduction Source: UniProtKB
    2. p38MAPK cascade Source: UniProtKB
    3. positive regulation of myoblast differentiation Source: UniProtKB
    4. positive regulation of myoblast fusion Source: UniProtKB
    5. positive regulation of myotube differentiation Source: UniProtKB
    6. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    7. response to stress Source: UniProtKB
    8. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Stress response, Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 14B (EC:2.7.11.24)
    Short name:
    MAP kinase 14B
    Short name:
    MAPK 14B
    Alternative name(s):
    Mitogen-activated protein kinase p38b
    Short name:
    MAP kinase p38b
    Short name:
    zp38b
    Gene namesi
    Name:mapk14b
    Synonyms:mapk14
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Unplaced

    Organism-specific databases

    ZFINiZDB-GENE-021007-1. mapk14b.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi181 – 1811T → A: Loss of enzyme activation. 1 Publication
    Mutagenesisi183 – 1831Y → F: Loss of enzyme activation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 348348Mitogen-activated protein kinase 14BPRO_0000186297Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei181 – 1811Phosphothreonine; by MAP2K31 Publication
    Modified residuei183 – 1831Phosphotyrosine; by MAP2K31 Publication

    Post-translational modificationi

    Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ9DGE1.

    Expressioni

    Developmental stagei

    Expressed at very low levels throughout development.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi7955.ENSDARP00000035686.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9DGE1.
    SMRiQ9DGE1. Positions 6-343.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 309285Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi181 – 1833TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOVERGENiHBG014652.
    KOiK04441.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008352. MAPK_p38.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01773. P38MAPKINASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9DGE1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQKARPTFY RQELNKTIWE VPERYQNLSP VGSGAYGSVM SAFDGKAGLR    50
    VAVKKLSRPF QSIIHAKRTY RELRLLKHMK HENVIGLLDV FSPATSLEGF 100
    NDVYLVTHLM GADLNNIVKC QKLTDDHVQF LIYQILRALK YIHSADIIHR 150
    DLKPSNLAVN EDCELKILDF GLARLTDDEM TGYVATRWYR APEIMLNWMH 200
    YNMTVDIWSV GCIMAELLTG RTLFPGTDHI NQLQQIMRLT GTPPASLISR 250
    MPSHEARNYI SSLPHMPKRN FADVFIGANP LAVDLLEKML VLDTDKRITA 300
    SQALAHPYFA QYHDPDDEPE ADPYDQSFES RDLEIEEWKS KIYIQNRN 348
    Length:348
    Mass (Da):39,998
    Last modified:March 1, 2001 - v1
    Checksum:i9922560377151EEE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB030898 mRNA. Translation: BAB11808.1.
    RefSeqiNP_835741.1. NM_178223.2.
    UniGeneiDr.81640.

    Genome annotation databases

    GeneIDi266756.
    KEGGidre:266756.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB030898 mRNA. Translation: BAB11808.1 .
    RefSeqi NP_835741.1. NM_178223.2.
    UniGenei Dr.81640.

    3D structure databases

    ProteinModelPortali Q9DGE1.
    SMRi Q9DGE1. Positions 6-343.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7955.ENSDARP00000035686.

    Proteomic databases

    PRIDEi Q9DGE1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 266756.
    KEGGi dre:266756.

    Organism-specific databases

    CTDi 266756.
    ZFINi ZDB-GENE-021007-1. mapk14b.

    Phylogenomic databases

    eggNOGi COG0515.
    HOVERGENi HBG014652.
    KOi K04441.

    Miscellaneous databases

    NextBioi 20804828.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008352. MAPK_p38.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01773. P38MAPKINASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Asymmetric p38 activation in zebrafish: its possible role in symmetric and synchronous cleavage."
      Fujii R., Yamashita S., Hibi M., Hirano T.
      J. Cell Biol. 150:1335-1348(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME REGULATION, PHOSPHORYLATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF THR-181 AND TYR-183.

    Entry informationi

    Entry nameiMK14B_DANRE
    AccessioniPrimary (citable) accession number: Q9DGE1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3