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Protein

Mitogen-activated protein kinase 14B

Gene

mapk14b

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Mapk14b is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation by the dual specificity kinase, MKK3.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1
Active sitei169Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 39ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processStress response, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24 928

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 14B (EC:2.7.11.24)
Short name:
MAP kinase 14B
Short name:
MAPK 14B
Alternative name(s):
Mitogen-activated protein kinase p38b
Short name:
MAP kinase p38b
Short name:
zp38b
Gene namesi
Name:mapk14b
Synonyms:mapk14
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-021007-1 mapk14b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi181T → A: Loss of enzyme activation. 1 Publication1
Mutagenesisi183Y → F: Loss of enzyme activation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001862971 – 348Mitogen-activated protein kinase 14BAdd BLAST348

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181Phosphothreonine; by MAP2K31 Publication1
Modified residuei183Phosphotyrosine; by MAP2K31 Publication1

Post-translational modificationi

Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9DGE1
PRIDEiQ9DGE1

PTM databases

iPTMnetiQ9DGE1

Expressioni

Developmental stagei

Expressed at very low levels throughout development.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000035686

Structurei

3D structure databases

ProteinModelPortaliQ9DGE1
SMRiQ9DGE1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 309Protein kinasePROSITE-ProRule annotationAdd BLAST285

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi181 – 183TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
HOVERGENiHBG014652
InParanoidiQ9DGE1

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR008352 MAPK_p38-like
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR01773 P38MAPKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

Q9DGE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQKARPTFY RQELNKTIWE VPERYQNLSP VGSGAYGSVM SAFDGKAGLR
60 70 80 90 100
VAVKKLSRPF QSIIHAKRTY RELRLLKHMK HENVIGLLDV FSPATSLEGF
110 120 130 140 150
NDVYLVTHLM GADLNNIVKC QKLTDDHVQF LIYQILRALK YIHSADIIHR
160 170 180 190 200
DLKPSNLAVN EDCELKILDF GLARLTDDEM TGYVATRWYR APEIMLNWMH
210 220 230 240 250
YNMTVDIWSV GCIMAELLTG RTLFPGTDHI NQLQQIMRLT GTPPASLISR
260 270 280 290 300
MPSHEARNYI SSLPHMPKRN FADVFIGANP LAVDLLEKML VLDTDKRITA
310 320 330 340
SQALAHPYFA QYHDPDDEPE ADPYDQSFES RDLEIEEWKS KIYIQNRN
Length:348
Mass (Da):39,998
Last modified:March 1, 2001 - v1
Checksum:i9922560377151EEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030898 mRNA Translation: BAB11808.1
UniGeneiDr.81640

Similar proteinsi

Entry informationi

Entry nameiMK14B_DANRE
AccessioniPrimary (citable) accession number: Q9DGE1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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