Q9DGE1 (MK14B_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitogen-activated protein kinase 14B
Short name=MAP kinase 14B
Short name=MAPK 14B
EC=2.7.11.24

Alternative name(s):
Mitogen-activated protein kinase p38b
Short name=MAP kinase p38b
Short name=zp38b

Gene names

Name:	mapk14b
Synonyms:	mapk14

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Mapk14b is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB Q90336
Cofactor	Magnesium By similarity. UniProtKB Q90336
Enzyme regulation	Activated by threonine and tyrosine phosphorylation by the dual specificity kinase, MKK3. Ref.1
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Developmental stage	Expressed at very low levels throughout development. Ref.1
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Stress response Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from sequence or structural similarity Ref.1. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from sequence or structural similarity Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Mitogen-activated protein kinase 14B		PRO_0000186297
Regions
	Domain	25 – 309	285	Protein kinase
	Nucleotide binding	31 – 39	9	ATP By similarity UniProtKB Q16539
	Motif	181 – 183	3	TXY
Sites
	Active site	169	1	Proton acceptor By similarity UniProtKB Q16539
	Binding site	54	1	ATP By similarity UniProtKB Q16539
Amino acid modifications
	Modified residue	181	1	Phosphothreonine; by MAP2K3 Ref.1
	Modified residue	183	1	Phosphotyrosine; by MAP2K3 Ref.1
Experimental info
	Mutagenesis	181	1	T → A: Loss of enzyme activation. Ref.1
	Mutagenesis	183	1	Y → F: Loss of enzyme activation. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9DGE1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 9922560377151EEE	FASTA	348	39,998
	10 20 30 40 50 60 MSQKARPTFY RQELNKTIWE VPERYQNLSP VGSGAYGSVM SAFDGKAGLR VAVKKLSRPF 70 80 90 100 110 120 QSIIHAKRTY RELRLLKHMK HENVIGLLDV FSPATSLEGF NDVYLVTHLM GADLNNIVKC 130 140 150 160 170 180 QKLTDDHVQF LIYQILRALK YIHSADIIHR DLKPSNLAVN EDCELKILDF GLARLTDDEM 190 200 210 220 230 240 TGYVATRWYR APEIMLNWMH YNMTVDIWSV GCIMAELLTG RTLFPGTDHI NQLQQIMRLT 250 260 270 280 290 300 GTPPASLISR MPSHEARNYI SSLPHMPKRN FADVFIGANP LAVDLLEKML VLDTDKRITA 310 320 330 340 SQALAHPYFA QYHDPDDEPE ADPYDQSFES RDLEIEEWKS KIYIQNRN

References

[1]	"Asymmetric p38 activation in zebrafish: its possible role in symmetric and synchronous cleavage." Fujii R., Yamashita S., Hibi M., Hirano T. J. Cell Biol. 150:1335-1348(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME REGULATION, PHOSPHORYLATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF THR-181 AND TYR-183.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB030898 mRNA. Translation: BAB11808.1.
IPI	IPI00865294.
RefSeq	NP_835741.1. NM_178223.2.
UniGene	Dr.81640.
3D structure databases
ProteinModelPortal	Q9DGE1.
SMR	Q9DGE1. Positions 6-343.
ModBase	Search...
Proteomic databases
PRIDE	Q9DGE1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	266756.
KEGG	dre:266756.
Organism-specific databases
CTD	266756.
ZFIN	ZDB-GENE-021007-1. mapk14b.
Phylogenomic databases
eggNOG	COG0515.
HOVERGEN	HBG014652.
KO	K04441.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR003527. MAP_kinase_CS. IPR008352. MAPK_p38. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01773. P38MAPKINASE.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20804828.

Entry information

Entry name

MK14B_DANRE

Accession

Primary (citable) accession number: Q9DGE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program