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Q9DGE1 (MK14B_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 14B

Short name=MAP kinase 14B
Short name=MAPK 14B
EC=2.7.11.24
Alternative name(s):
Mitogen-activated protein kinase p38b
Short name=MAP kinase p38b
Short name=zp38b
Gene names
Name:mapk14b
Synonyms:mapk14
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Mapk14b is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. UniProtKB Q90336

Cofactor

Magnesium By similarity. UniProtKB Q90336

Enzyme regulation

Activated by threonine and tyrosine phosphorylation by the dual specificity kinase, MKK3. Ref.1

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Developmental stage

Expressed at very low levels throughout development. Ref.1

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processStress response
Transcription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processintracellular signal transduction

Inferred from sequence or structural similarity Ref.1. Source: UniProtKB

p38MAPK cascade

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of myoblast differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of myoblast fusion

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of myotube differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: UniProtKB

response to stress

Inferred from sequence or structural similarity Ref.1. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from sequence or structural similarity Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348Mitogen-activated protein kinase 14B
PRO_0000186297

Regions

Domain25 – 309285Protein kinase
Nucleotide binding31 – 399ATP By similarity UniProtKB Q16539
Motif181 – 1833TXY

Sites

Active site1691Proton acceptor By similarity UniProtKB Q16539
Binding site541ATP By similarity UniProtKB Q16539

Amino acid modifications

Modified residue1811Phosphothreonine; by MAP2K3 Ref.1
Modified residue1831Phosphotyrosine; by MAP2K3 Ref.1

Experimental info

Mutagenesis1811T → A: Loss of enzyme activation. Ref.1
Mutagenesis1831Y → F: Loss of enzyme activation. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9DGE1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 9922560377151EEE

FASTA34839,998
        10         20         30         40         50         60 
MSQKARPTFY RQELNKTIWE VPERYQNLSP VGSGAYGSVM SAFDGKAGLR VAVKKLSRPF 

        70         80         90        100        110        120 
QSIIHAKRTY RELRLLKHMK HENVIGLLDV FSPATSLEGF NDVYLVTHLM GADLNNIVKC 

       130        140        150        160        170        180 
QKLTDDHVQF LIYQILRALK YIHSADIIHR DLKPSNLAVN EDCELKILDF GLARLTDDEM 

       190        200        210        220        230        240 
TGYVATRWYR APEIMLNWMH YNMTVDIWSV GCIMAELLTG RTLFPGTDHI NQLQQIMRLT 

       250        260        270        280        290        300 
GTPPASLISR MPSHEARNYI SSLPHMPKRN FADVFIGANP LAVDLLEKML VLDTDKRITA 

       310        320        330        340 
SQALAHPYFA QYHDPDDEPE ADPYDQSFES RDLEIEEWKS KIYIQNRN 

« Hide

References

[1]"Asymmetric p38 activation in zebrafish: its possible role in symmetric and synchronous cleavage."
Fujii R., Yamashita S., Hibi M., Hirano T.
J. Cell Biol. 150:1335-1348(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME REGULATION, PHOSPHORYLATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF THR-181 AND TYR-183.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB030898 mRNA. Translation: BAB11808.1.
RefSeqNP_835741.1. NM_178223.2.
UniGeneDr.81640.

3D structure databases

ProteinModelPortalQ9DGE1.
SMRQ9DGE1. Positions 6-343.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000035686.

Proteomic databases

PRIDEQ9DGE1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID266756.
KEGGdre:266756.

Organism-specific databases

CTD266756.
ZFINZDB-GENE-021007-1. mapk14b.

Phylogenomic databases

eggNOGCOG0515.
HOVERGENHBG014652.
KOK04441.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01773. P38MAPKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20804828.

Entry information

Entry nameMK14B_DANRE
AccessionPrimary (citable) accession number: Q9DGE1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families