Reviewed, UniProtKB/Swiss-Prot Q9DGE0 (MP2K6_DANRE)

Names and origin

Protein names

Recommended name:
    Dual specificity mitogen-activated protein kinase kinase 6
      Short name=MAP kinase kinase 6
      Short name=MAPKK 6
    EC=2.7.12.2
Alternative name(s):
    Mitogen-activated protein kinase kinase 3
      Short name=zMKK3

Gene names

Name:	map2k6
Synonyms:	map2k3

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively. This phosphorylation asymmetrically activates p38 on one side of the blastodisc, an event which is necessary for blastomere cleavage. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Developmental stage	Expressed both maternally and zygotically. Zygotic expression continues throughout development. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.
Caution	Was originally (Ref.1) thought to be a Map2k3 protein but sequence analysis shows closer similarity to the Map2k6 proteins.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Dual specificity mitogen-activated protein kinase kinase 6		PRO_0000345633
Regions
	Domain	80 – 341	262	Protein kinase
	Nucleotide binding	86 – 94	9	ATP By similarity
Sites
	Active site	206	1	Proton acceptor By similarity
	Binding site	109	1	ATP
Experimental info
	Mutagenesis	109	1	K → A: Acts as dominant negative. Inhibits activation of MAP kinase p38 and disrupts cleavage. Ref.1
	Mutagenesis	234 – 238	5	SVAKT → EVAKE: Constitutively active. Ref.1
	Sequence conflict	28	1	S → SNV in BAB11809. Ref.1
	Sequence conflict	28	1	S → SNV in AAH44129. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9DGE0-1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: D942485DE14864B9	FASTA	361	40,594
	10 20 30 40 50 60 MEGGSDKESK VFCDSPSPNP KGEMSVPSNV RGKKKLPKEL KLPKEVFEKP APAPTPPRDL 70 80 90 100 110 120 DSKAYVTIGE KNFVVKADDL EQIGELGRGA YGVVDKMRHV PSGVIMAVKR IRATVNTQEQ 130 140 150 160 170 180 KRLLMDLDIS MRTVDCFYTV TFYGALFREG DVWICMELMD TSLDKFYKQV HEKGKTIPED 190 200 210 220 230 240 ILGKITVSIV KALEHLHSNL SVIHRDVKPS NVLINMQGQV KMCDFGISGY LVDSVAKTMD 250 260 270 280 290 300 AGCKPYMAPE RINPETNQKG YNVKSDIWSL GITMIELAIL RFPYDSWGTP FQQLKQVVEE 310 320 330 340 350 360 PSPQLPADRF SADFVDFTSQ CLRKNSTERP TYTELMQHPF FTLHDSKDTD VASFVKTILG D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Asymmetric p38 activation in zebrafish: its possible role in symmetric and synchronous cleavage." Fujii R., Yamashita S., Hibi M., Hirano T. J. Cell Biol. 150:1335-1348(2000) [PubMed: 10995439] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-109 AND 234-SER--THR-238. Tissue: Embryo.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: AB. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB030899 mRNA. Translation: BAB11809.1. BC044129 mRNA. Translation: AAH44129.1. BC071285 mRNA. Translation: AAH71285.1. Different initiation.
IPI	IPI00480961.
RefSeq	NP_571799.1.
UniGene	Dr.117346
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSDART00000021857; ENSDARP00000018191; ENSDARG00000005567; Danio rerio. [Genome view]
GeneID	65239.
KEGG	dre:65239.
Organism-specific databases
CTD	65239.
ZFIN	ZDB-GENE-010202-3. map2k6.
Phylogenomic databases
HOGENOM	Q9DGE0.
HOVERGEN	Q9DGE0.
Gene expression databases
Bgee	Q9DGE0.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MP2K6_DANRE

Accession

Primary (citable) accession number: Q9DGE0
Secondary accession number(s): Q6IQW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	September 1, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project