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Q9DGD9

- MK08_DANRE

UniProt

Q9DGD9 - MK08_DANRE

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Protein
Mitogen-activated protein kinase 8
Gene
mapk8, jnk1
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity By similarity.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium By similarity.By similarity

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei55 – 551ATP By similarityBy similarity
Active sitei151 – 1511Proton acceptor By similarityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi33 – 386ATP By similarityBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. JNK cascade Source: UniProtKB
  2. determination of dorsal identity Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_180916. Oxidative Stress Induced Senescence.
REACT_181665. FCERI mediated MAPK activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 8 (EC:2.7.11.24)
Short name:
MAP kinase 8
Short name:
MAPK 8
Alternative name(s):
Stress-activated protein kinase JNK1
c-Jun N-terminal kinase 1
Gene namesi
Name:mapk8
Synonyms:jnk1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 12

Organism-specific databases

ZFINiZDB-GENE-010202-1. mapk8b.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 384384Mitogen-activated protein kinase 8
PRO_0000186265Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei183 – 1831Phosphothreonine By similarityBy similarity
Modified residuei185 – 1851Phosphotyrosine By similarityBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9DGD9.

Expressioni

Developmental stagei

Expressed in the nervous system during embryogenic stage 21. Expression decreases from stage 25 onwards.1 Publication

Gene expression databases

BgeeiQ9DGD9.

Structurei

3D structure databases

ProteinModelPortaliQ9DGD9.
SMRiQ9DGD9. Positions 10-364.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 321296Protein kinaseBy similarity
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi183 – 1853TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074271.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ9DGD9.
KOiK04440.
OrthoDBiEOG7PCJGV.
PhylomeDBiQ9DGD9.
TreeFamiTF105100.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01772. JNKMAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DGD9-1 [UniParc]FASTAAdd to Basket

« Hide

MNRNKREKEY YSIDVGDSTF TVLKRYQNLR PIGSGAQGIV CSAYDHVLDR    50
NVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKTLEE 100
FQDVYLVMEL MDANLCQVIQ MELDHERLSY LLYQMLCGIK HLHAAGIIHR 150
DLKPSNIVVK SDCTLKILDF GLARTAATGL LMTPYVVTRY YRAPEVILGM 200
GYQANVDVWS IGCIMAEMVR GSVLFPGTDH IDQWNKVIEQ LGTPSQEFMM 250
KLNQSVRTYV ENRPRYAGYS FEKLFPDVLF PADSDHNKLK ASQARDLLSK 300
MLVIDASKRI SVDEALQHPY INVWYDPSEV EAPPPAITDK QLDEREHSVE 350
EWKELIYKEV LEWEERTKNG VIRGQPASLA QVQQ 384
Length:384
Mass (Da):44,140
Last modified:March 1, 2001 - v1
Checksum:i9596EE6183D2A6BC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB030900 mRNA. Translation: BAB11810.1.
RefSeqiNP_571796.1. NM_131721.1.
UniGeneiDr.82505.

Genome annotation databases

EnsembliENSDART00000022471; ENSDARP00000012611; ENSDARG00000009870.
GeneIDi65236.
KEGGidre:65236.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB030900 mRNA. Translation: BAB11810.1 .
RefSeqi NP_571796.1. NM_131721.1.
UniGenei Dr.82505.

3D structure databases

ProteinModelPortali Q9DGD9.
SMRi Q9DGD9. Positions 10-364.
ModBasei Search...

Proteomic databases

PRIDEi Q9DGD9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000022471 ; ENSDARP00000012611 ; ENSDARG00000009870 .
GeneIDi 65236.
KEGGi dre:65236.

Organism-specific databases

CTDi 65236.
ZFINi ZDB-GENE-010202-1. mapk8b.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074271.
HOGENOMi HOG000233024.
HOVERGENi HBG014652.
InParanoidi Q9DGD9.
KOi K04440.
OrthoDBi EOG7PCJGV.
PhylomeDBi Q9DGD9.
TreeFami TF105100.

Enzyme and pathway databases

Reactomei REACT_180916. Oxidative Stress Induced Senescence.
REACT_181665. FCERI mediated MAPK activation.

Miscellaneous databases

NextBioi 20902033.
PROi Q9DGD9.

Gene expression databases

Bgeei Q9DGD9.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01772. JNKMAPKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Asymmetric p38 activation in zebrafish: its possible role in symmetric and synchronous cleavage."
    Fujii R., Yamashita S., Hibi M., Hirano T.
    J. Cell Biol. 150:1335-1348(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Tissue: Embryo.

Entry informationi

Entry nameiMK08_DANRE
AccessioniPrimary (citable) accession number: Q9DGD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2001
Last modified: September 3, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi