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Protein

Mitogen-activated protein kinase 8

Gene

mapk8

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. May play a role in the regulation of the circadian clock.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei55 – 551ATPPROSITE-ProRule annotation
Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi33 – 386ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • determination of dorsal identity Source: MGI
  • JNK cascade Source: UniProtKB
  • rhythmic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Biological rhythms

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 928.
ReactomeiREACT_287625. Activation of the AP-1 family of transcription factors.
REACT_302200. DSCAM interactions.
REACT_304857. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
REACT_305705. FCERI mediated MAPK activation.
REACT_316017. NRIF signals cell death from the nucleus.
REACT_331584. Oxidative Stress Induced Senescence.
REACT_347331. NRAGE signals death through JNK.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 8 (EC:2.7.11.24)
Short name:
MAP kinase 8
Short name:
MAPK 8
Alternative name(s):
Stress-activated protein kinase JNK1
c-Jun N-terminal kinase 1
Gene namesi
Name:mapk8
Synonyms:jnk1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437 Componenti: Chromosome 12

Organism-specific databases

ZFINiZDB-GENE-010202-1. mapk8b.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: GOC
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 384384Mitogen-activated protein kinase 8PRO_0000186265Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei183 – 1831PhosphothreonineBy similarity
Modified residuei185 – 1851PhosphotyrosineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9DGD9.

Expressioni

Developmental stagei

Expressed in the nervous system during embryogenic stage 21. Expression decreases from stage 25 onwards.1 Publication

Gene expression databases

BgeeiQ9DGD9.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000111769.

Structurei

3D structure databases

ProteinModelPortaliQ9DGD9.
SMRiQ9DGD9. Positions 10-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 321296Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi183 – 1853TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074271.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ9DGD9.
KOiK04440.
OrthoDBiEOG7PCJGV.
PhylomeDBiQ9DGD9.
TreeFamiTF105100.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01772. JNKMAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DGD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRNKREKEY YSIDVGDSTF TVLKRYQNLR PIGSGAQGIV CSAYDHVLDR
60 70 80 90 100
NVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKTLEE
110 120 130 140 150
FQDVYLVMEL MDANLCQVIQ MELDHERLSY LLYQMLCGIK HLHAAGIIHR
160 170 180 190 200
DLKPSNIVVK SDCTLKILDF GLARTAATGL LMTPYVVTRY YRAPEVILGM
210 220 230 240 250
GYQANVDVWS IGCIMAEMVR GSVLFPGTDH IDQWNKVIEQ LGTPSQEFMM
260 270 280 290 300
KLNQSVRTYV ENRPRYAGYS FEKLFPDVLF PADSDHNKLK ASQARDLLSK
310 320 330 340 350
MLVIDASKRI SVDEALQHPY INVWYDPSEV EAPPPAITDK QLDEREHSVE
360 370 380
EWKELIYKEV LEWEERTKNG VIRGQPASLA QVQQ
Length:384
Mass (Da):44,140
Last modified:March 1, 2001 - v1
Checksum:i9596EE6183D2A6BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030900 mRNA. Translation: BAB11810.1.
RefSeqiNP_571796.1. NM_131721.1.
UniGeneiDr.82505.

Genome annotation databases

EnsembliENSDART00000022471; ENSDARP00000012611; ENSDARG00000009870.
GeneIDi65236.
KEGGidre:65236.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030900 mRNA. Translation: BAB11810.1.
RefSeqiNP_571796.1. NM_131721.1.
UniGeneiDr.82505.

3D structure databases

ProteinModelPortaliQ9DGD9.
SMRiQ9DGD9. Positions 10-364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000111769.

Proteomic databases

PRIDEiQ9DGD9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000022471; ENSDARP00000012611; ENSDARG00000009870.
GeneIDi65236.
KEGGidre:65236.

Organism-specific databases

CTDi65236.
ZFINiZDB-GENE-010202-1. mapk8b.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074271.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ9DGD9.
KOiK04440.
OrthoDBiEOG7PCJGV.
PhylomeDBiQ9DGD9.
TreeFamiTF105100.

Enzyme and pathway databases

BRENDAi2.7.11.24. 928.
ReactomeiREACT_287625. Activation of the AP-1 family of transcription factors.
REACT_302200. DSCAM interactions.
REACT_304857. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
REACT_305705. FCERI mediated MAPK activation.
REACT_316017. NRIF signals cell death from the nucleus.
REACT_331584. Oxidative Stress Induced Senescence.
REACT_347331. NRAGE signals death through JNK.

Miscellaneous databases

NextBioi20902033.
PROiQ9DGD9.

Gene expression databases

BgeeiQ9DGD9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01772. JNKMAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Asymmetric p38 activation in zebrafish: its possible role in symmetric and synchronous cleavage."
    Fujii R., Yamashita S., Hibi M., Hirano T.
    J. Cell Biol. 150:1335-1348(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Tissue: Embryo.

Entry informationi

Entry nameiMK08_DANRE
AccessioniPrimary (citable) accession number: Q9DGD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2001
Last modified: June 24, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.