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Q9DGD9

- MK08_DANRE

UniProt

Q9DGD9 - MK08_DANRE

Protein

Mitogen-activated protein kinase 8

Gene

mapk8

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei55 – 551ATPPROSITE-ProRule annotation
    Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi33 – 386ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. determination of dorsal identity Source: MGI
    2. JNK cascade Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_180916. Oxidative Stress Induced Senescence.
    REACT_181665. FCERI mediated MAPK activation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 8 (EC:2.7.11.24)
    Short name:
    MAP kinase 8
    Short name:
    MAPK 8
    Alternative name(s):
    Stress-activated protein kinase JNK1
    c-Jun N-terminal kinase 1
    Gene namesi
    Name:mapk8
    Synonyms:jnk1
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 12

    Organism-specific databases

    ZFINiZDB-GENE-010202-1. mapk8b.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 384384Mitogen-activated protein kinase 8PRO_0000186265Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei183 – 1831PhosphothreonineBy similarity
    Modified residuei185 – 1851PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ9DGD9.

    Expressioni

    Developmental stagei

    Expressed in the nervous system during embryogenic stage 21. Expression decreases from stage 25 onwards.1 Publication

    Gene expression databases

    BgeeiQ9DGD9.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9DGD9.
    SMRiQ9DGD9. Positions 10-364.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 321296Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi183 – 1853TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000074271.
    HOGENOMiHOG000233024.
    HOVERGENiHBG014652.
    InParanoidiQ9DGD9.
    KOiK04440.
    OrthoDBiEOG7PCJGV.
    PhylomeDBiQ9DGD9.
    TreeFamiTF105100.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008351. MAPK_JNK.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01772. JNKMAPKINASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9DGD9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRNKREKEY YSIDVGDSTF TVLKRYQNLR PIGSGAQGIV CSAYDHVLDR    50
    NVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKTLEE 100
    FQDVYLVMEL MDANLCQVIQ MELDHERLSY LLYQMLCGIK HLHAAGIIHR 150
    DLKPSNIVVK SDCTLKILDF GLARTAATGL LMTPYVVTRY YRAPEVILGM 200
    GYQANVDVWS IGCIMAEMVR GSVLFPGTDH IDQWNKVIEQ LGTPSQEFMM 250
    KLNQSVRTYV ENRPRYAGYS FEKLFPDVLF PADSDHNKLK ASQARDLLSK 300
    MLVIDASKRI SVDEALQHPY INVWYDPSEV EAPPPAITDK QLDEREHSVE 350
    EWKELIYKEV LEWEERTKNG VIRGQPASLA QVQQ 384
    Length:384
    Mass (Da):44,140
    Last modified:March 1, 2001 - v1
    Checksum:i9596EE6183D2A6BC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB030900 mRNA. Translation: BAB11810.1.
    RefSeqiNP_571796.1. NM_131721.1.
    UniGeneiDr.82505.

    Genome annotation databases

    EnsembliENSDART00000022471; ENSDARP00000012611; ENSDARG00000009870.
    GeneIDi65236.
    KEGGidre:65236.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB030900 mRNA. Translation: BAB11810.1 .
    RefSeqi NP_571796.1. NM_131721.1.
    UniGenei Dr.82505.

    3D structure databases

    ProteinModelPortali Q9DGD9.
    SMRi Q9DGD9. Positions 10-364.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9DGD9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000022471 ; ENSDARP00000012611 ; ENSDARG00000009870 .
    GeneIDi 65236.
    KEGGi dre:65236.

    Organism-specific databases

    CTDi 65236.
    ZFINi ZDB-GENE-010202-1. mapk8b.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000074271.
    HOGENOMi HOG000233024.
    HOVERGENi HBG014652.
    InParanoidi Q9DGD9.
    KOi K04440.
    OrthoDBi EOG7PCJGV.
    PhylomeDBi Q9DGD9.
    TreeFami TF105100.

    Enzyme and pathway databases

    Reactomei REACT_180916. Oxidative Stress Induced Senescence.
    REACT_181665. FCERI mediated MAPK activation.

    Miscellaneous databases

    NextBioi 20902033.
    PROi Q9DGD9.

    Gene expression databases

    Bgeei Q9DGD9.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008351. MAPK_JNK.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01772. JNKMAPKINASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Asymmetric p38 activation in zebrafish: its possible role in symmetric and synchronous cleavage."
      Fujii R., Yamashita S., Hibi M., Hirano T.
      J. Cell Biol. 150:1335-1348(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
      Tissue: Embryo.

    Entry informationi

    Entry nameiMK08_DANRE
    AccessioniPrimary (citable) accession number: Q9DGD9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3