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Q9DGD9 (MK08_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 8

Short name=MAP kinase 8
Short name=MAPK 8
EC=2.7.11.24
Alternative name(s):
Stress-activated protein kinase JNK1
c-Jun N-terminal kinase 1
Gene names
Name:mapk8
Synonyms:jnk1
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length384 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity By similarity. UniProtKB P45983

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity. UniProtKB P45983

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity. UniProtKB P45983

Developmental stage

Expressed in the nervous system during embryogenic stage 21. Expression decreases from stage 25 onwards. Ref.1

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 384384Mitogen-activated protein kinase 8
PRO_0000186265

Regions

Domain26 – 321296Protein kinase UniProtKB P53779
Nucleotide binding33 – 386ATP By similarity UniProtKB P53779
Motif183 – 1853TXY

Sites

Active site1511Proton acceptor By similarity UniProtKB P53779
Binding site551ATP By similarity UniProtKB P53779

Amino acid modifications

Modified residue1831Phosphothreonine By similarity UniProtKB P53779
Modified residue1851Phosphotyrosine By similarity UniProtKB P53779

Sequences

Sequence LengthMass (Da)Tools
Q9DGD9 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 9596EE6183D2A6BC

FASTA38444,140
        10         20         30         40         50         60 
MNRNKREKEY YSIDVGDSTF TVLKRYQNLR PIGSGAQGIV CSAYDHVLDR NVAIKKLSRP 

        70         80         90        100        110        120 
FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKTLEE FQDVYLVMEL MDANLCQVIQ 

       130        140        150        160        170        180 
MELDHERLSY LLYQMLCGIK HLHAAGIIHR DLKPSNIVVK SDCTLKILDF GLARTAATGL 

       190        200        210        220        230        240 
LMTPYVVTRY YRAPEVILGM GYQANVDVWS IGCIMAEMVR GSVLFPGTDH IDQWNKVIEQ 

       250        260        270        280        290        300 
LGTPSQEFMM KLNQSVRTYV ENRPRYAGYS FEKLFPDVLF PADSDHNKLK ASQARDLLSK 

       310        320        330        340        350        360 
MLVIDASKRI SVDEALQHPY INVWYDPSEV EAPPPAITDK QLDEREHSVE EWKELIYKEV 

       370        380 
LEWEERTKNG VIRGQPASLA QVQQ 

« Hide

References

[1]"Asymmetric p38 activation in zebrafish: its possible role in symmetric and synchronous cleavage."
Fujii R., Yamashita S., Hibi M., Hirano T.
J. Cell Biol. 150:1335-1348(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB030900 mRNA. Translation: BAB11810.1.
RefSeqNP_571796.1. NM_131721.1.
UniGeneDr.82505.

3D structure databases

ProteinModelPortalQ9DGD9.
SMRQ9DGD9. Positions 10-364.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9DGD9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000022471; ENSDARP00000012611; ENSDARG00000009870.
GeneID65236.
KEGGdre:65236.

Organism-specific databases

CTD65236.
ZFINZDB-GENE-010202-1. mapk8b.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000074271.
HOGENOMHOG000233024.
HOVERGENHBG014652.
InParanoidQ9DGD9.
KOK04440.
OrthoDBEOG7PCJGV.
PhylomeDBQ9DGD9.
TreeFamTF105100.

Gene expression databases

BgeeQ9DGD9.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01772. JNKMAPKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20902033.
PROQ9DGD9.

Entry information

Entry nameMK08_DANRE
AccessionPrimary (citable) accession number: Q9DGD9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families