Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9DGD9

- MK08_DANRE

UniProt

Q9DGD9 - MK08_DANRE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Mitogen-activated protein kinase 8

Gene

mapk8

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium.By similarity

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei55 – 551ATPPROSITE-ProRule annotation
Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi33 – 386ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. determination of dorsal identity Source: MGI
  2. JNK cascade Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_180916. Oxidative Stress Induced Senescence.
REACT_181665. FCERI mediated MAPK activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 8 (EC:2.7.11.24)
Short name:
MAP kinase 8
Short name:
MAPK 8
Alternative name(s):
Stress-activated protein kinase JNK1
c-Jun N-terminal kinase 1
Gene namesi
Name:mapk8
Synonyms:jnk1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 12

Organism-specific databases

ZFINiZDB-GENE-010202-1. mapk8b.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 384384Mitogen-activated protein kinase 8PRO_0000186265Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei183 – 1831PhosphothreonineBy similarity
Modified residuei185 – 1851PhosphotyrosineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9DGD9.

Expressioni

Developmental stagei

Expressed in the nervous system during embryogenic stage 21. Expression decreases from stage 25 onwards.1 Publication

Gene expression databases

BgeeiQ9DGD9.

Structurei

3D structure databases

ProteinModelPortaliQ9DGD9.
SMRiQ9DGD9. Positions 10-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 321296Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi183 – 1853TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074271.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ9DGD9.
KOiK04440.
OrthoDBiEOG7PCJGV.
PhylomeDBiQ9DGD9.
TreeFamiTF105100.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01772. JNKMAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DGD9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNRNKREKEY YSIDVGDSTF TVLKRYQNLR PIGSGAQGIV CSAYDHVLDR
60 70 80 90 100
NVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKTLEE
110 120 130 140 150
FQDVYLVMEL MDANLCQVIQ MELDHERLSY LLYQMLCGIK HLHAAGIIHR
160 170 180 190 200
DLKPSNIVVK SDCTLKILDF GLARTAATGL LMTPYVVTRY YRAPEVILGM
210 220 230 240 250
GYQANVDVWS IGCIMAEMVR GSVLFPGTDH IDQWNKVIEQ LGTPSQEFMM
260 270 280 290 300
KLNQSVRTYV ENRPRYAGYS FEKLFPDVLF PADSDHNKLK ASQARDLLSK
310 320 330 340 350
MLVIDASKRI SVDEALQHPY INVWYDPSEV EAPPPAITDK QLDEREHSVE
360 370 380
EWKELIYKEV LEWEERTKNG VIRGQPASLA QVQQ
Length:384
Mass (Da):44,140
Last modified:March 1, 2001 - v1
Checksum:i9596EE6183D2A6BC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB030900 mRNA. Translation: BAB11810.1.
RefSeqiNP_571796.1. NM_131721.1.
UniGeneiDr.82505.

Genome annotation databases

EnsembliENSDART00000022471; ENSDARP00000012611; ENSDARG00000009870.
GeneIDi65236.
KEGGidre:65236.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB030900 mRNA. Translation: BAB11810.1 .
RefSeqi NP_571796.1. NM_131721.1.
UniGenei Dr.82505.

3D structure databases

ProteinModelPortali Q9DGD9.
SMRi Q9DGD9. Positions 10-364.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9DGD9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000022471 ; ENSDARP00000012611 ; ENSDARG00000009870 .
GeneIDi 65236.
KEGGi dre:65236.

Organism-specific databases

CTDi 65236.
ZFINi ZDB-GENE-010202-1. mapk8b.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074271.
HOGENOMi HOG000233024.
HOVERGENi HBG014652.
InParanoidi Q9DGD9.
KOi K04440.
OrthoDBi EOG7PCJGV.
PhylomeDBi Q9DGD9.
TreeFami TF105100.

Enzyme and pathway databases

Reactomei REACT_180916. Oxidative Stress Induced Senescence.
REACT_181665. FCERI mediated MAPK activation.

Miscellaneous databases

NextBioi 20902033.
PROi Q9DGD9.

Gene expression databases

Bgeei Q9DGD9.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01772. JNKMAPKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Asymmetric p38 activation in zebrafish: its possible role in symmetric and synchronous cleavage."
    Fujii R., Yamashita S., Hibi M., Hirano T.
    J. Cell Biol. 150:1335-1348(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Tissue: Embryo.

Entry informationi

Entry nameiMK08_DANRE
AccessioniPrimary (citable) accession number: Q9DGD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3