Reviewed, UniProtKB/Swiss-Prot Q9DGD1 (MGT4C_CHICK)

Names and origin

Protein names

Recommended name:
    Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C
    EC=2.4.1.145
    EC=2.4.1.-
Alternative name(s):
    UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVc
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVc
      Short name=N-acetylglucosaminyltransferase IVc
      Short name=GnT-IVc
    N-acetylglucosaminyltransferase VI
      Short name=GnT-VI

Gene names

Name:

MGAT4C

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Glycosyltransferase that catalyzes the transfer of GlcNAc to the Manalpha1-6 arm to form GlcNAcBeta1-4Manalpha1-6 linkage (also named 'GnT-VI' activity). May also participate in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans by catalyzing the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Ref.1
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R. Ref.1 Ref.3
Cofactor	Divalent metal cations. Ref.2
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	Highly expressed in oviduct, spleen, lung and colon. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 54 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C		PRO_0000288600
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 31	21	Signal-anchor for type II membrane protein Potential
	Topological domain	32 – 464	433	Lumenal Potential
Amino acid modifications
	Glycosylation	70	1	N-linked (GlcNAc...) Potential
	Glycosylation	201	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9DGD1-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 80946781FE1A7CA8	FASTA	464	52,820
	10 20 30 40 50 60 MRCSPKRSLT AVIAASFLLL LLLLLLHRGS WQDPQEVQFR DLPSDAVLKI LKQGSLHILQ 70 80 90 100 110 120 DTDNLCALHN ISYHLLAGSP LPHKKFLAVG LSSVRRPRGY YLPDTLQSLF KQSSEEELQE 130 140 150 160 170 180 MVVVVHLADA DPIWNAQVAA DISHRFAHHI LLGRLVLIHT PHEFYPTLEG LKRNYNDPEE 190 200 210 220 230 240 RVKFRSKQNV DYAFLFTFAA NLSSYYLMIE DDVWSAKSFF TAIRKAVASQ EGSNWATLEF 250 260 270 280 290 300 SKLGYIGKLY RSSDLPRLAR FLLLFYQEMP CDWLLTHFRL LLTQKDVIRF KPSLFQHMGL 310 320 330 340 350 360 YSSFQGTVNR LEDDEFQADA MDLPDNPPAA LFTNMVVFEN YEPSKAYSTA RGYFWGKNPA 370 380 390 400 410 420 VGSIFSIVFH QPARVTRVRV QTGSSERPGD FLHAGVLELG RGRRADGRDC SVYTTVGTFE 430 440 450 460 KGNLEWRGLE KGMPNPVECV RIRVTQSQSE WLIIQSIGIW TAGT

References

[1]	"Molecular cloning and expression of cDNA encoding chicken UDP-N-acetyl-D-glucosamine (GlcNAc): GlcNAcbeta 1-6(GlcNAcbeta 1-2)-manalpha 1-R[GlcNAc to man]beta 1,4N-acetylglucosaminyltransferase VI." Sakamoto Y., Taguchi T., Honke K., Korekane H., Watanabe H., Tano Y., Dohmae N., Takio K., Horii A., Taniguchi N. J. Biol. Chem. 275:36029-36034(2000) [PubMed: 10962001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 85-107; 173-183; 358-384 AND 422-431, FUNCTION, ENZYME ACTIVITY, TISSUE SPECIFICITY.
[2]	"Purification and characterization of UDP-GlcNAc: GlcNAcbeta 1-6(GlcNAcbeta 1-2)Manalpha 1-R [GlcNAc to Man]-beta 1, 4-N-acetylglucosaminyltransferase VI from hen oviduct." Taguchi T., Ogawa T., Inoue S., Inoue Y., Sakamoto Y., Korekane H., Taniguchi N. J. Biol. Chem. 275:32598-32602(2000) [PubMed: 10903319] [Abstract] Cited for: IDENTIFICATION, COFACTOR.
[3]	"A specific detection of GlcNAcbeta1-6Manalpha1 branches in N-linked glycoproteins based on the specificity of N-acetylglucosaminyltransferase VI." Watanabe T., Ihara H., Miyoshi E., Honke K., Taniguchi N., Taguchi T. Glycobiology 16:431-439(2006) [PubMed: 16428802] [Abstract] Cited for: ENZYME ACTIVITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB040608 mRNA. Translation: BAB16845.1.
IPI	IPI00586690.
RefSeq	NP_990012.1.
UniGene	Gga.2933
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT54. Glycosyltransferase Family 54.
Proteomic databases
PRIDE	Q9DGD1.
Genome annotation databases
Ensembl	ENSGALG00000000225. Gallus gallus. [Contig view]
GeneID	395415.
KEGG	gga:395415.
Phylogenomic databases
HOGENOM	Q9DGD1.
HOVERGEN	Q9DGD1.
Enzyme and pathway databases
BRENDA	2.4.1.145. 4. 2.4.1.201. 4.
Family and domain databases
InterPro	IPR006759. Glyco_transf_54. [Graphical view]
PANTHER	PTHR12062. Glyco_transf_54. 1 hit.
Pfam	PF04666. Glyco_transf_54. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGT4C_CHICK

Accession

Primary (citable) accession number: Q9DGD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 1, 2001
Last modified:	April 14, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)