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Q9DGA2 (CDK1_ORYJA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 1

Short name=CDK1
EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
Cell division control protein 2 homolog
Cell division protein kinase 1
p34 protein kinase
Gene names
Name:cdk1
Synonyms:cdc2
OrganismOryzias javanicus (Javanese ricefish)
Taxonomic identifier123683 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataOvalentariaAtherinomorphaeBeloniformesAdrianichthyidaeOryziinaeOryzias

Protein attributes

Sequence length303 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Catalytic component of MPF By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulation

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it By similarity.

Subunit structure

Forms a stable but non-covalent complex with cyclin B in mature oocytes By similarity.

Subcellular location

Nucleus By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity.

Post-translational modification

Phosphorylation at Tyr-15 by wee1 and wee2 inhibits the protein kinase activity and acts negative regulator of entry into mitosis (G2 to M transition) By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 303303Cyclin-dependent kinase 1
PRO_0000085731

Regions

Domain4 – 287284Protein kinase
Nucleotide binding10 – 189ATP By similarity

Sites

Active site1281Proton acceptor By similarity
Binding site331ATP By similarity

Amino acid modifications

Modified residue141Phosphothreonine By similarity
Modified residue151Phosphotyrosine; by wee1 and wee2 By similarity
Modified residue1611Phosphothreonine; by cak By similarity

Natural variations

Natural variant491V → I.
Natural variant2451M → K.
Natural variant2691I → T.
Natural variant2761I → V.
Natural variant2991C → S.

Sequences

Sequence LengthMass (Da)Tools
Q9DGA2 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 439B8B012BE28D9C

FASTA30334,691
        10         20         30         40         50         60 
MEDYVKIEKI GEGTYGVVYK GRHKSTGQVV AMKKIRLESE EEGVPSTAVR EVSLLQELKH 

        70         80         90        100        110        120 
PNVVRLLDVL MQESRLYLIF EFLSMDLKKY LDSIPSGQYM DPMLVKSYLY QILEGIYFCH 

       130        140        150        160        170        180 
RRRVLHRDLK PQNLLIDNKG VIKLADFGLA RAFGVPVRVY THEVVTLWYR APEVLLGSPR 

       190        200        210        220        230        240 
YSTPVDVWST GTIFAELATK KPLFHGDSEI DQLFRIFRTL GTPNNDVWPD VESLPDYKNT 

       250        260        270        280        290        300 
FPKWMEGSLS SMVKNLDKNG LDLLAKMLIY NPPKRISARE AMTHPYFDDL DKSTLPAACI 


NGV 

« Hide

References

[1]"cDNA cloning of Cdc2 and cyclin B in medaka species."
Yamashita M., Mita K.
Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB050461 mRNA. Translation: BAB17219.1.
AB050462 mRNA. Translation: BAB17220.1.

3D structure databases

ProteinModelPortalQ9DGA2.
SMRQ9DGA2. Positions 1-292.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9DGA2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG014652.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCDK1_ORYJA
AccessionPrimary (citable) accession number: Q9DGA2
Secondary accession number(s): Q9DGA1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2001
Last modified: June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families