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Q9DGA2

- CDK1_ORYJA

UniProt

Q9DGA2 - CDK1_ORYJA

Protein

Cyclin-dependent kinase 1

Gene

cdk1

Organism
Oryzias javanicus (Javanese ricefish)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Catalytic component of MPF By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Enzyme regulationi

    Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Active sitei128 – 1281Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
    3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. regulation of cell cycle Source: GOC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
    Short name:
    CDK1
    Alternative name(s):
    Cell division control protein 2 homolog
    Cell division protein kinase 1
    p34 protein kinase
    Gene namesi
    Name:cdk1
    Synonyms:cdc2
    OrganismiOryzias javanicus (Javanese ricefish)
    Taxonomic identifieri123683 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataOvalentariaAtherinomorphaeBeloniformesAdrianichthyidaeOryziinaeOryzias

    Subcellular locationi

    Nucleus By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. microtubule organizing center Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 303303Cyclin-dependent kinase 1PRO_0000085731Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei14 – 141PhosphothreonineBy similarity
    Modified residuei15 – 151Phosphotyrosine; by wee1 and wee2By similarity
    Modified residuei161 – 1611Phosphothreonine; by cakBy similarity

    Post-translational modificationi

    Phosphorylation at Tyr-15 by wee1 and wee2 inhibits the protein kinase activity and acts negative regulator of entry into mitosis (G2 to M transition).By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ9DGA2.

    Interactioni

    Subunit structurei

    Forms a stable but non-covalent complex with cyclin B in mature oocytes.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9DGA2.
    SMRiQ9DGA2. Positions 1-292.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 287284Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG014652.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9DGA2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDYVKIEKI GEGTYGVVYK GRHKSTGQVV AMKKIRLESE EEGVPSTAVR    50
    EVSLLQELKH PNVVRLLDVL MQESRLYLIF EFLSMDLKKY LDSIPSGQYM 100
    DPMLVKSYLY QILEGIYFCH RRRVLHRDLK PQNLLIDNKG VIKLADFGLA 150
    RAFGVPVRVY THEVVTLWYR APEVLLGSPR YSTPVDVWST GTIFAELATK 200
    KPLFHGDSEI DQLFRIFRTL GTPNNDVWPD VESLPDYKNT FPKWMEGSLS 250
    SMVKNLDKNG LDLLAKMLIY NPPKRISARE AMTHPYFDDL DKSTLPAACI 300
    NGV 303
    Length:303
    Mass (Da):34,691
    Last modified:March 1, 2001 - v1
    Checksum:i439B8B012BE28D9C
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti49 – 491V → I.
    Natural varianti245 – 2451M → K.
    Natural varianti269 – 2691I → T.
    Natural varianti276 – 2761I → V.
    Natural varianti299 – 2991C → S.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB050461 mRNA. Translation: BAB17219.1.
    AB050462 mRNA. Translation: BAB17220.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB050461 mRNA. Translation: BAB17219.1 .
    AB050462 mRNA. Translation: BAB17220.1 .

    3D structure databases

    ProteinModelPortali Q9DGA2.
    SMRi Q9DGA2. Positions 1-292.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9DGA2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG014652.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning of Cdc2 and cyclin B in medaka species."
      Yamashita M., Mita K.
      Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Ovary.

    Entry informationi

    Entry nameiCDK1_ORYJA
    AccessioniPrimary (citable) accession number: Q9DGA2
    Secondary accession number(s): Q9DGA1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 27, 2002
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3