Reviewed, UniProtKB/Swiss-Prot Q9DGA2 (CDC2_ORYJA)

Names and origin

Protein names

Recommended name:
    Cell division control protein 2 homolog
    EC=2.7.11.22
    EC=2.7.11.23
Alternative name(s):
    p34 protein kinase
    Cyclin-dependent kinase 1
      Short name=CDK1

Gene names

Name:

cdc2

Organism

Oryzias javanicus (Javanese ricefish)

Taxonomic identifier

123683 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Atherinomorpha › Beloniformes › Adrianichthyidae › Oryziinae › Oryzias

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Catalytic component of MPF By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Enzyme regulation	Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it By similarity.
Subunit structure	Forms a stable but non-covalent complex with cyclin B in mature oocytes By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Cell division control protein 2 homolog		PRO_0000085731
Regions
	Domain	4 – 287	284	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	128	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Amino acid modifications
	Modified residue	14	1	Phosphothreonine By similarity
	Modified residue	15	1	Phosphotyrosine By similarity
	Modified residue	161	1	Phosphothreonine; by CAK By similarity
Natural variations
	Natural variant	49	1	V → I
	Natural variant	245	1	M → K
	Natural variant	269	1	I → T
	Natural variant	276	1	I → V
	Natural variant	299	1	C → S

Sequences

	Sequence		Length	Mass (Da)
	Q9DGA2-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 439B8B012BE28D9C	FASTA	303	34,691
	10 20 30 40 50 60 MEDYVKIEKI GEGTYGVVYK GRHKSTGQVV AMKKIRLESE EEGVPSTAVR EVSLLQELKH 70 80 90 100 110 120 PNVVRLLDVL MQESRLYLIF EFLSMDLKKY LDSIPSGQYM DPMLVKSYLY QILEGIYFCH 130 140 150 160 170 180 RRRVLHRDLK PQNLLIDNKG VIKLADFGLA RAFGVPVRVY THEVVTLWYR APEVLLGSPR 190 200 210 220 230 240 YSTPVDVWST GTIFAELATK KPLFHGDSEI DQLFRIFRTL GTPNNDVWPD VESLPDYKNT 250 260 270 280 290 300 FPKWMEGSLS SMVKNLDKNG LDLLAKMLIY NPPKRISARE AMTHPYFDDL DKSTLPAACI NGV

References

[1]	"cDNA cloning of Cdc2 and cyclin B in medaka species." Yamashita M., Mita K. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB050461 mRNA. Translation: BAB17219.1. AB050462 mRNA. Translation: BAB17220.1.
3D structure databases
SMR	Q9DGA2. Positions 1-292.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q9DGA2.
Enzyme and pathway databases
BRENDA	2.7.11.22. 303585. 2.7.11.23. 303585.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDC2_ORYJA

Accession

Primary (citable) accession number: Q9DGA2
Secondary accession number(s): Q9DGA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)