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Protein

Proliferating cell nuclear antigen

Gene

PCNA

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi61 – 8020Sequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-GGA-110312. Translesion synthesis by REV1.
R-GGA-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-GGA-110320. Translesion Synthesis by POLH.
R-GGA-113510. E2F mediated regulation of DNA replication.
R-GGA-1538133. G0 and Early G1.
R-GGA-174411. Polymerase switching on the C-strand of the telomere.
R-GGA-174437. Removal of the Flap Intermediate from the C-strand.
R-GGA-353299. RAD18 and ubiquitinated PCNA-mediated recruitment of translesion polymerases.
R-GGA-353303. Nucleotide Excision Repair.
R-GGA-4615885. SUMOylation of DNA replication proteins.
R-GGA-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-GGA-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-GGA-5655862. Translesion synthesis by POLK.
R-GGA-5656121. Translesion synthesis by POLI.
R-GGA-5656169. Termination of translesion DNA synthesis.
R-GGA-5685942. HDR through Homologous Recombination (HRR).
R-GGA-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-GGA-5696400. Dual Incision in GG-NER.
R-GGA-6782135. Dual incision in TC-NER.
R-GGA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-GGA-69091. Polymerase switching.
R-GGA-69166. Removal of the Flap Intermediate.
R-GGA-69205. G1/S-Specific Transcription.

Names & Taxonomyi

Protein namesi
Recommended name:
Proliferating cell nuclear antigen
Short name:
PCNA
Gene namesi
Name:PCNA
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 22

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262Proliferating cell nuclear antigenPRO_0000149163Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki164 – 164Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Monoubiquitinated by the UBE2B-RAD18 complex on Lys-164. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9DEA3.
PRIDEiQ9DEA3.

Interactioni

Subunit structurei

Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with DNMT1 (By similarity). Interacts with CHAF1A.By similarity2 Publications

Protein-protein interaction databases

BioGridi675031. 1 interaction.
STRINGi9031.ENSGALP00000040305.

Structurei

3D structure databases

ProteinModelPortaliQ9DEA3.
SMRiQ9DEA3. Positions 1-259.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PCNA family.Curated

Phylogenomic databases

eggNOGiKOG1636. Eukaryota.
COG0592. LUCA.
GeneTreeiENSGT00390000004965.
HOGENOMiHOG000211098.
HOVERGENiHBG000947.
InParanoidiQ9DEA3.
KOiK04802.
OMAiSDGFDKY.
OrthoDBiEOG7JX34W.
PhylomeDBiQ9DEA3.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
TIGRFAMsiTIGR00590. pcna. 1 hit.
PROSITEiPS01251. PCNA_1. 1 hit.
PS00293. PCNA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DEA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEARLVQGS VLKRVLEALK DLITEACWDL GSGGISLQSM DSSHVSLVQL
60 70 80 90 100
TLRSEGFDTY RCDRNIAMGV NLNSMSKILK CAGNEDIITL RAEDNADTLA
110 120 130 140 150
LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SAEFARICRD
160 170 180 190 200
LSHIGDAVVI SCAKDGVKFS ANGELGNGNI KLSQTSNVDK EEEAVTIEMN
210 220 230 240 250
EPVQLTFALR YLNFFTKATP LSPTVTLSMS ADVPLVVEYK IADMGHLKYY
260
LAPKIEDQQE GS
Length:262
Mass (Da):28,888
Last modified:March 1, 2001 - v1
Checksum:iBD5B14D7D302D0B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053163 mRNA. Translation: BAB20424.1.
RefSeqiNP_989501.1. NM_204170.2.
UniGeneiGga.4036.

Genome annotation databases

EnsembliENSGALT00000000225; ENSGALP00000000224; ENSGALG00000000169.
GeneIDi373984.
KEGGigga:373984.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053163 mRNA. Translation: BAB20424.1.
RefSeqiNP_989501.1. NM_204170.2.
UniGeneiGga.4036.

3D structure databases

ProteinModelPortaliQ9DEA3.
SMRiQ9DEA3. Positions 1-259.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi675031. 1 interaction.
STRINGi9031.ENSGALP00000040305.

Proteomic databases

PaxDbiQ9DEA3.
PRIDEiQ9DEA3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000000225; ENSGALP00000000224; ENSGALG00000000169.
GeneIDi373984.
KEGGigga:373984.

Organism-specific databases

CTDi5111.

Phylogenomic databases

eggNOGiKOG1636. Eukaryota.
COG0592. LUCA.
GeneTreeiENSGT00390000004965.
HOGENOMiHOG000211098.
HOVERGENiHBG000947.
InParanoidiQ9DEA3.
KOiK04802.
OMAiSDGFDKY.
OrthoDBiEOG7JX34W.
PhylomeDBiQ9DEA3.

Enzyme and pathway databases

ReactomeiR-GGA-110312. Translesion synthesis by REV1.
R-GGA-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-GGA-110320. Translesion Synthesis by POLH.
R-GGA-113510. E2F mediated regulation of DNA replication.
R-GGA-1538133. G0 and Early G1.
R-GGA-174411. Polymerase switching on the C-strand of the telomere.
R-GGA-174437. Removal of the Flap Intermediate from the C-strand.
R-GGA-353299. RAD18 and ubiquitinated PCNA-mediated recruitment of translesion polymerases.
R-GGA-353303. Nucleotide Excision Repair.
R-GGA-4615885. SUMOylation of DNA replication proteins.
R-GGA-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-GGA-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-GGA-5655862. Translesion synthesis by POLK.
R-GGA-5656121. Translesion synthesis by POLI.
R-GGA-5656169. Termination of translesion DNA synthesis.
R-GGA-5685942. HDR through Homologous Recombination (HRR).
R-GGA-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-GGA-5696400. Dual Incision in GG-NER.
R-GGA-6782135. Dual incision in TC-NER.
R-GGA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-GGA-69091. Polymerase switching.
R-GGA-69166. Removal of the Flap Intermediate.
R-GGA-69205. G1/S-Specific Transcription.

Miscellaneous databases

NextBioi20813513.
PROiQ9DEA3.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
TIGRFAMsiTIGR00590. pcna. 1 hit.
PROSITEiPS01251. PCNA_1. 1 hit.
PS00293. PCNA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Co-localization of chicken DNA topoisomerase IIalpha, but not beta, with sites of DNA replication and possible involvement of a C-terminal region of alpha through its binding to PCNA."
    Niimi A., Suka N., Harata M., Kikuchi A., Mizuno S.
    Chromosoma 110:102-114(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], IN VITRO BINDING TO TOP2A.
  2. "Human DNA-(cytosine-5) methyltransferase-PCNA complex as a target for p21WAF1."
    Chuang L.S.-H., Ian H.-I., Koh T.-W., Ng H.-H., Xu G., Li B.F.L.
    Science 277:1996-2000(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DNMT1.
  3. "Essential role of chromatin assembly factor-1-mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells."
    Takami Y., Ono T., Fukagawa T., Shibahara K., Nakayama T.
    Mol. Biol. Cell 18:129-141(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CHAF1A.

Entry informationi

Entry nameiPCNA_CHICK
AccessioniPrimary (citable) accession number: Q9DEA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 1, 2001
Last modified: March 16, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.