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Protein

Serine/threonine-protein kinase atr

Gene

atr

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mn2+By similarity

Enzyme regulationi

Kinase activity is activated by topbp1.1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • protein heterodimerization activity Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  • DNA repair Source: UniProtKB-KW
  • peptidyl-serine phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Manganese, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase atr (EC:2.7.11.1)
Short name:
Xatr
Alternative name(s):
Ataxia telangiectasia and Rad3-related protein
Gene namesi
Name:atr
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-1012725. atr.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 26542654Serine/threonine-protein kinase atrPRO_0000225628Add
BLAST

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiQ9DE14.

Interactioni

Subunit structurei

Forms a heterodimer with atrip. Interacts with topbp1 in the presence of atrip.1 Publication

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi99532. 3 interactions.
IntActiQ9DE14. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9DE14.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati814 – 85037HEAT 1Add
BLAST
Repeati1344 – 138037HEAT 2Add
BLAST
Domaini1654 – 2196543FATPROSITE-ProRule annotationAdd
BLAST
Domaini2331 – 2577247PI3K/PI4KPROSITE-ProRule annotationAdd
BLAST
Domaini2622 – 265433FATCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family. ATM subfamily.Curated
Contains 1 FAT domain.PROSITE-ProRule annotation
Contains 1 FATC domain.PROSITE-ProRule annotation
Contains 2 HEAT repeats.Curated
Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG050619.
KOiK06640.

Family and domain databases

Gene3Di1.10.1070.11. 2 hits.
1.25.10.10. 2 hits.
1.25.40.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR003152. FATC_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003151. PIK-rel_kinase_FAT.
IPR014009. PIK_FAT.
IPR011990. TPR-like_helical_dom.
IPR012993. UME.
[Graphical view]
PfamiPF02259. FAT. 1 hit.
PF02260. FATC. 1 hit.
PF00454. PI3_PI4_kinase. 1 hit.
PF08064. UME. 1 hit.
[Graphical view]
SMARTiSM01343. FATC. 1 hit.
SM00146. PI3Kc. 1 hit.
SM00802. UME. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 6 hits.
SSF48452. SSF48452. 2 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS51189. FAT. 1 hit.
PS51190. FATC. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DE14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATDPGLEMA SMIPALRELA SAGAEEYNTT VQKPRQILCQ FIDRILTDVD
60 70 80 90 100
VVAVELSKNT DSQPSSVMLL DFIQHIMKST PLMFLSANNG DQSAETNQNC
110 120 130 140 150
VAFSNWIISR LLRIGATPSC KALHRKIAEV IRSLLFLFKN KSSFLFGVFT
160 170 180 190 200
KDLLHLFEDL IYIHEQNMEK SVVWPVTISR FLSNASENQT YLRCTQFQLL
210 220 230 240 250
NMQNIEPLES TLLMVLMDNE HDISPVFFQR QNLLLWGIGC SLLDYGSTPL
260 270 280 290 300
KIQALHFLRQ LIKLGGPPEQ GAYFFFIVFF GILTCIKDMD LEEVSLYEMP
310 320 330 340 350
LLKLVKVLFP FESKSYLNIE PVYLNMLLEK LAALFDGGIL SNIQSAPLKE
360 370 380 390 400
ALCYMVHYFL SIVPPGYESA KEVREAHVRC ICRAFVDVLG LQSKQEYLVC
410 420 430 440 450
PLHEALRIEN LVFMQQQRMQ PLSTDSEGGG SSSSDEVQEK RPRLSLTAKP
460 470 480 490 500
LRRNTPSVPA PVDMKTKSIL WKAVSAKFSS ILCKLEGDEV TDEEMVSLLE
510 520 530 540 550
GLNTTVRVAA LNTVHIFTND STDTDQLVSD LSNTSGIQSV EIVPHVFWLS
560 570 580 590 600
PEDILKILKI CRKVLDSAHQ RANINDILMK IIKIFDAILY IHAGNRLNDQ
610 620 630 640 650
TLKDLCSMIS LPWLQNHSNH ASFKVASFDP TLMTISERIG QHYSPEIQSQ
660 670 680 690 700
LVFLLCLFPK MLCPEWRLAV YQWALDSPHE IVRARCIKGF PVLLCNVSQQ
710 720 730 740 750
GYGPIPKILI DCLNDASELV KKELANSVGM FASGLACGFE LQYSPTAPTA
760 770 780 790 800
AESEFLCSSL TVTALPSSKL SRMTASALKP FLALLNRNMP SSVKMAFIEN
810 820 830 840 850
MPMLFAHLSL EKDDLDSRTV IESLLNLMED PDKDVRTAFS GNIKHLLACA
860 870 880 890 900
DCEDGYLKEI VVSRMKKAYT DAKMSRDNEM KDTLILTTGD IGRAAKGELV
910 920 930 940 950
PFALLHLLHC LLSKSPCVAG ASYTEIRSLA AAKSTSLHIF FSQYKKPICQ
960 970 980 990 1000
FLIESLHSSQ AALLTNTPGR SSEMQKQEAT HHREAALDIL SEIANVFDFP
1010 1020 1030 1040 1050
DLNRFLTRTL QLLLPYLAAK ASPTASTLIR TIAKQLNVNR REILINNFKY
1060 1070 1080 1090 1100
IFSHLVCSCT KDELEKSLHY LKNETEIELG SLLRQDYQGL HNELLLRLGE
1110 1120 1130 1140 1150
HYQQVFSGLS ILATYASNDD PYQGPRNFAK PEIMADYLQP KLLGILAFFN
1160 1170 1180 1190 1200
MHLLSSSIGI EDKKMALNSL VSLMKLMGPK HISSVRVKMM TTLRTGLRYK
1210 1220 1230 1240 1250
EEFPGLCCSA WDLFVRCLDQ AYLGPLLSHV IVALLPLLHI QPKETVAVFR
1260 1270 1280 1290 1300
YLIVENRDAV QDFLHEIYFL PDHPELKEIQ KVLQEYRKET TKSTDLQTAM
1310 1320 1330 1340 1350
QLSIRAIQHE NVDVRMHALT SLKETLYKNQ AKLLQYSTDS ETVEPVISQL
1360 1370 1380 1390 1400
VTVLLIGCQD ANPQARLFCG ECLGQLGAID PGRLDFSPSE TQGKGFTFVS
1410 1420 1430 1440 1450
GVEDSDFAYE LLTEQTRAFL AYADNVRAQD SAAYAIQELL SIFECKEGRT
1460 1470 1480 1490 1500
DCPGRRLWRR FPEHVQEILE PHLNTRYKSS RKAVNWSRVK KPIYLSKLGN
1510 1520 1530 1540 1550
NFADWSATWA GYLITKVRHE LARRVFSCCS IMMKHDFKVT IYLLPHILVY
1560 1570 1580 1590 1600
VLLGCNKEDQ QEVYAEIMAV LKHEDPLMRR LQDSASDLSQ LSTQTVFSML
1610 1620 1630 1640 1650
DHLTQWAREK FQALNAEKTN PKPGTRGEPK AVSNEDYGEY QNVTRFLDLI
1660 1670 1680 1690 1700
PQDTLAVASF RSKAYTRALM HFESFIMEKK QEIQEHLGFL QKLYAAMHEP
1710 1720 1730 1740 1750
DGVAGVSAIR KKEASLKEQI LEHESIGLLR DATACYDRAI QLKPEEIIHY
1760 1770 1780 1790 1800
HGVVKSMLGL GQLSTVITQV NGILNSRSEW TAELNTYRVE AAWKLSQWDL
1810 1820 1830 1840 1850
VEEYLSADRK STTWSIRLGQ LLLSAKKGER DMFYETLKVV RAEQIVPLSA
1860 1870 1880 1890 1900
ASFERGSYQR GYEYIVRLHM LCELEHSVKM FLQKPSVEPA VDSLNLPARL
1910 1920 1930 1940 1950
EMTQNSYRAR EPILAVRRAL QTINKRPNHA DMIGECWLQS ARVARKAGHH
1960 1970 1980 1990 2000
QTAYNALLNA GESRLSELNV ERAKWLWSKG DVHQALIVLQ KGAELFLSST
2010 2020 2030 2040 2050
SAPPEQQLIH GRAMLLVGRL MEETANFESN AVMKKYKDVT ALLPEWEDGH
2060 2070 2080 2090 2100
FYLAKYYDKL MPMVTDNKME KQGDLIRYIV LHFGRSLQFG NQYIYQSMPR
2110 2120 2130 2140 2150
MLSLWLDFGA KVYEWEKAGR ADRLQMKNEL MKINKVISDH KNQLAPYQFL
2160 2170 2180 2190 2200
TAFSQLISRI CHSHDEVFAV LMEIVAKVFV AYPQQAMWMM TAVSKSSYPM
2210 2220 2230 2240 2250
RVNRCKEILE KAIHMKPSLG KFIGDATRLT DKLLELCNKP VDGNTSTLSM
2260 2270 2280 2290 2300
NIHFKMLKKL VEETTFSEIL IPLQSVMIPT LPSTAGKRDH ADHDPFPGHW
2310 2320 2330 2340 2350
AYLSGFDDAV EILPSLQKPK KISLKGSDGK SYIMMCKPKD DLRKDCRLME
2360 2370 2380 2390 2400
FNSLINKCLR KDAESRRREL HIRTYAVIPL NDECGIIEWV NNTAGFRNIL
2410 2420 2430 2440 2450
IKLYKEKGIY MGGKELRQCM LPKSAPLQEK LKVFKEALLP RHPPLFHEWF
2460 2470 2480 2490 2500
LRTFPDPTSW YNSRSAYCRS TAVMSMVGYI LGLGDRHGEN ILFDSLTGEC
2510 2520 2530 2540 2550
VHVDFNCLFN KGETFEVPEI VPFRLTHNMV NGMGPMGTEG LFRRACEVIM
2560 2570 2580 2590 2600
RLMREQRESL MSVLKPFLHD PLVEWSKPAR GSSKGQVNET GEVMNEKAKT
2610 2620 2630 2640 2650
HVLDIEQRLQ GVIKTRNRVK GLPLSIEGHV HYLIQEATDE NLLSQMYLGW

APYM
Length:2,654
Mass (Da):301,459
Last modified:March 7, 2006 - v2
Checksum:iF88E8A6541E6DD13
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti191 – 1911Y → C in AAG34794 (PubMed:11069891).Curated
Sequence conflicti249 – 2491P → S in AAG34794 (PubMed:11069891).Curated
Sequence conflicti434 – 4341S → SS in AAW78662 (Ref. 3) Curated
Sequence conflicti520 – 5201D → N in AAW78662 (Ref. 3) Curated
Sequence conflicti1047 – 10471N → S in AAW78662 (Ref. 3) Curated
Sequence conflicti1543 – 15431L → P in AAW78662 (Ref. 3) Curated
Sequence conflicti1785 – 17851N → S in AAG40002 (PubMed:11137007).Curated
Sequence conflicti1952 – 19521T → A in AAW78662 (Ref. 3) Curated
Sequence conflicti2082 – 20821H → P in AAG34794 (PubMed:11069891).Curated
Sequence conflicti2215 – 22151M → T in AAW78662 (Ref. 3) Curated
Sequence conflicti2362 – 23621D → G in AAG34794 (PubMed:11069891).Curated
Sequence conflicti2424 – 24241S → N in AAG40002 (PubMed:11137007).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF320125 mRNA. Translation: AAG40002.1.
AF223644 mRNA. Translation: AAG34794.1.
AY882989 mRNA. Translation: AAW78662.1.
RefSeqiNP_001082049.1. NM_001088580.1.
UniGeneiXl.718.

Genome annotation databases

GeneIDi398197.
KEGGixla:398197.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF320125 mRNA. Translation: AAG40002.1.
AF223644 mRNA. Translation: AAG34794.1.
AY882989 mRNA. Translation: AAW78662.1.
RefSeqiNP_001082049.1. NM_001088580.1.
UniGeneiXl.718.

3D structure databases

ProteinModelPortaliQ9DE14.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi99532. 3 interactions.
IntActiQ9DE14. 1 interaction.

PTM databases

iPTMnetiQ9DE14.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi398197.
KEGGixla:398197.

Organism-specific databases

CTDi545.
XenbaseiXB-GENE-1012725. atr.

Phylogenomic databases

HOVERGENiHBG050619.
KOiK06640.

Family and domain databases

Gene3Di1.10.1070.11. 2 hits.
1.25.10.10. 2 hits.
1.25.40.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR003152. FATC_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003151. PIK-rel_kinase_FAT.
IPR014009. PIK_FAT.
IPR011990. TPR-like_helical_dom.
IPR012993. UME.
[Graphical view]
PfamiPF02259. FAT. 1 hit.
PF02260. FATC. 1 hit.
PF00454. PI3_PI4_kinase. 1 hit.
PF08064. UME. 1 hit.
[Graphical view]
SMARTiSM01343. FATC. 1 hit.
SM00146. PI3Kc. 1 hit.
SM00802. UME. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 6 hits.
SSF48452. SSF48452. 2 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS51189. FAT. 1 hit.
PS51190. FATC. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATR_XENLA
AccessioniPrimary (citable) accession number: Q9DE14
Secondary accession number(s): Q5EGL8, Q9DDK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: March 7, 2006
Last modified: May 11, 2016
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.