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Protein

Sphingosine 1-phosphate receptor 1

Gene

s1pr1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate.1 Publication

GO - Molecular functioni

GO - Biological processi

  • cell migration involved in gastrulation Source: ZFIN
  • chemotaxis Source: UniProtKB-KW
  • G-protein coupled receptor signaling pathway Source: UniProtKB
  • mesodermal cell migration Source: ZFIN
  • negative regulation of angiogenesis Source: ZFIN
  • positive regulation of cell migration Source: ZFIN
  • regulation of blood vessel remodeling Source: ZFIN
  • sphingosine-1-phosphate signaling pathway Source: UniProtKB
  • sprouting angiogenesis Source: ZFIN
  • vasculature development Source: ZFIN

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processChemotaxis

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine 1-phosphate receptor 1
Short name:
S1P receptor 1
Short name:
S1P1
Alternative name(s):
Sphingosine 1-phosphate receptor Edg-1
Short name:
S1P receptor Edg-1
Gene namesi
Name:s1pr1
Synonyms:edg1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-001228-2. s1pr1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 25ExtracellularBy similarityAdd BLAST25
Transmembranei26 – 47Helical; Name=1By similarityAdd BLAST22
Topological domaini48 – 61CytoplasmicBy similarityAdd BLAST14
Transmembranei62 – 83Helical; Name=2By similarityAdd BLAST22
Topological domaini84 – 95ExtracellularBy similarityAdd BLAST12
Transmembranei96 – 117Helical; Name=3By similarityAdd BLAST22
Topological domaini118 – 139CytoplasmicBy similarityAdd BLAST22
Transmembranei140 – 161Helical; Name=4By similarityAdd BLAST22
Topological domaini162 – 175ExtracellularBy similarityAdd BLAST14
Transmembranei176 – 203Helical; Name=5By similarityAdd BLAST28
Topological domaini204 – 238CytoplasmicBy similarityAdd BLAST35
Transmembranei239 – 259Helical; Name=6By similarityAdd BLAST21
Topological domaini260 – 270ExtracellularBy similarityAdd BLAST11
Transmembranei271 – 291Helical; Name=7By similarityAdd BLAST21
Topological domaini292 – 362CytoplasmicBy similarityAdd BLAST71

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000694151 – 362Sphingosine 1-phosphate receptor 1Add BLAST362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi10N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi163 ↔ 170PROSITE-ProRule annotation
Glycosylationi169N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi263 ↔ 268PROSITE-ProRule annotation
Lipidationi309S-palmitoyl cysteineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9DDK4.

Expressioni

Developmental stagei

Embryonic brain. Not detected during blastula or gastrula stages. At the 4-5 somite stage, expressed in the diencephalon just posterior to the optic vesicles, and weakly in the hindbrain. By the 14 somite stage, there is widespread expression in the CNS, particularly in the ventral diencephalon, optic stalks and anterior hindbrain.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000062632.

Structurei

3D structure databases

ProteinModelPortaliQ9DDK4.
SMRiQ9DDK4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni99 – 100Sphingosine 1-phosphate bindingBy similarity2
Regioni246 – 250Sphingosine 1-phosphate bindingBy similarity5

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIA7. Eukaryota.
ENOG410XQD3. LUCA.
HOGENOMiHOG000233501.
HOVERGENiHBG103071.
InParanoidiQ9DDK4.
KOiK04288.
PhylomeDBiQ9DDK4.

Family and domain databases

InterProiView protein in InterPro
IPR000987. EDG1_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004061. S1P_rcpt.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PRINTSiPR00642. EDG1RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01523. S1PRECEPTOR.
SMARTiView protein in SMART
SM01381. 7TM_GPCR_Srsx. 1 hit.
PROSITEiView protein in PROSITE
PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9DDK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDLIARHYN FTGKFRKVHK DPGLKADSVV FIIVCCFIIL ENVLVLLTIW
60 70 80 90 100
RTKKFHKPMY YFIGNLALSD LLAGVVYTAN ILLSGANTYK LTPTQWFFRE
110 120 130 140 150
GSMFVALAAS VFSLLAIAIE RHLTMLKMKL HNNGKTCRVF MLISTVWFIA
160 170 180 190 200
AILGGLPVMG WNCIDSMNNC STVLPLYHKA YILFCTTVFS VILMAIVILY
210 220 230 240 250
ARIYALVRTR SRKLVFRKVA NGRGSNKSSE KSMALLKTVI IVLSCFIACW
260 270 280 290 300
APLFILLLLD VACQTLTCSI LYKAEWFLAL AVLNSAMNPL IYTLTSNEMR
310 320 330 340 350
RAFIKMLNCG VCVQPSGKFS RPIMGAEFSR SKSDNSSHPN KDEPEYSPRE
360
TIVSSGNITS SS
Length:362
Mass (Da):40,601
Last modified:February 15, 2005 - v2
Checksum:iEF779D90CF6108C3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167M → I in AAG45430 (PubMed:11112429).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF321294 mRNA. Translation: AAG45430.1.
BC075741 mRNA. Translation: AAH75741.1.
PIRiJC7559.
RefSeqiNP_571766.3. NM_131691.3.
UniGeneiDr.77753.

Genome annotation databases

GeneIDi64617.
KEGGidre:64617.

Similar proteinsi

Entry informationi

Entry nameiS1PR1_DANRE
AccessioniPrimary (citable) accession number: Q9DDK4
Secondary accession number(s): Q6DI49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: May 10, 2017
This is version 93 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families