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Protein

SUMO-conjugating enzyme UBC9-B

Gene

ube2ib

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin-like proteins sumo1, sumo2 and sumo3 from the uble1a-uble1b E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as ranbp2 or cbx4. Essential for nuclear architecture and chromosome segregation (By similarity). Mediates nuclear localization of vsx1. Required for progression through mitosis during organogenesis.By similarity2 Publications

Catalytic activityi

SUMOyl-[E2 SUMO-conjugating enzyme]-L-cysteine + [acceptor-protein]-L-lysine = [E2 SUMO-conjugating enzyme]-L-cysteine + N6-SUMOyl-[acceptor protein]-L-lysine.

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei93Glycyl thioester intermediatePROSITE-ProRule annotation1
Sitei100 – 101Substrate bindingBy similarity2

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: UniProtKB-KW
  • definitive hemopoiesis Source: ZFIN
  • embryonic heart tube elongation Source: ZFIN
  • heart looping Source: ZFIN

Keywordsi

Molecular functionDevelopmental protein, Transferase
Biological processCell cycle, Cell division, Chromosome partition, Mitosis, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DRE-196791 Vitamin D (calciferol) metabolism
R-DRE-3065678 SUMO is transferred from E1 to E2 (UBE2I, UBC9)
R-DRE-3108214 SUMOylation of DNA damage response and repair proteins
R-DRE-3232118 SUMOylation of transcription factors
R-DRE-3899300 SUMOylation of transcription cofactors
R-DRE-4551638 SUMOylation of chromatin organization proteins
R-DRE-4570464 SUMOylation of RNA binding proteins
R-DRE-4615885 SUMOylation of DNA replication proteins
R-DRE-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-DRE-5693607 Processing of DNA double-strand break ends
R-DRE-5696395 Formation of Incision Complex in GG-NER
R-DRE-8866904 Negative regulation of activity of TFAP2 (AP-2) family transcription factors
UniPathwayiUPA00886

Names & Taxonomyi

Protein namesi
Recommended name:
SUMO-conjugating enzyme UBC9-B (EC:2.3.2.-)
Alternative name(s):
RING-type E3 SUMO transferase UBC9-B
SUMO-protein ligase B
Ubiquitin carrier protein 9-B
Ubiquitin carrier protein I-B
Ubiquitin-conjugating enzyme E2 I-B
Ubiquitin-protein ligase I-B
Gene namesi
Name:ube2ib
Synonyms:ubc9b, ube2i2
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 3

Organism-specific databases

ZFINiZDB-GENE-010607-1 ube2ia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002688771 – 157SUMO-conjugating enzyme UBC9-BAdd BLAST157

Proteomic databases

PaxDbiQ9DDJ0
PRIDEiQ9DDJ0

Expressioni

Gene expression databases

BgeeiENSDARG00000052649
ExpressionAtlasiQ9DDJ0 baseline

Interactioni

Subunit structurei

Forms a tight complex with rangap1 and ranbp2 (By similarity). Interacts with vsx1.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei4Interaction with RANBP2By similarity1
Sitei25Interaction with RANBP2By similarity1
Sitei57Interaction with RANBP2By similarity1

Protein-protein interaction databases

STRINGi7955.ENSDARP00000052745

Structurei

3D structure databases

ProteinModelPortaliQ9DDJ0
SMRiQ9DDJ0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 18Interaction with SUMO1By similarity6

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0424 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00550000075088
HOGENOMiHOG000233454
HOVERGENiHBG063308
InParanoidiQ9DDJ0
KOiK10577
OMAiKCKFTPP
OrthoDBiEOG091G0NAK
PhylomeDBiQ9DDJ0
TreeFamiTF101122

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR027230 Ubc9
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PANTHERiPTHR43927 PTHR43927, 1 hit
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9DDJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG
60 70 80 90 100
TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED
110 120 130 140 150
KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA

QAKKFSP
Length:157
Mass (Da):17,936
Last modified:March 1, 2001 - v1
Checksum:iF83E89C8D0683DFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF332623 mRNA Translation: AAG48365.1
BC066609 mRNA Translation: AAH66609.1
BC058302 mRNA Translation: AAH58302.1
RefSeqiNP_571908.1, NM_131833.2
UniGeneiDr.112951

Genome annotation databases

EnsembliENSDART00000052746; ENSDARP00000052745; ENSDARG00000052649
GeneIDi114445
KEGGidre:114445

Similar proteinsi

Entry informationi

Entry nameiUBC9B_DANRE
AccessioniPrimary (citable) accession number: Q9DDJ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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