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Protein

D-aminoacyl-tRNA deacylase 1

Gene

Dtd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.By similarity
ATPase involved in DNA replication, may facilitate loading of CDC45 onto pre-replication complexes.By similarity

Catalytic activityi

Glycyl-tRNA(Ala) + H2O = glycine + tRNA(Ala).By similarity
A D-aminoacyl-tRNA + H2O = a D-amino acid + tRNA.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Hydrolase, RNA-binding, tRNA-binding
Biological processDNA replication
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
D-aminoacyl-tRNA deacylase 1By similarity (EC:3.1.1.96By similarity)
Short name:
DTD
Alternative name(s):
DNA-unwinding element-binding protein B
Short name:
DUE-B
Gly-tRNA(Ala) deacylaseBy similarity (EC:3.1.1.-By similarity)
Gene namesi
Name:Dtd1
Synonyms:Hars2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1913294. Dtd1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001646271 – 209D-aminoacyl-tRNA deacylase 1Add BLAST209

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei197PhosphoserineCombined sources1
Modified residuei204PhosphoserineCombined sources1
Modified residuei205PhosphoserineCombined sources1

Post-translational modificationi

Preferentially phosphorylated in cells arrested early in S phase. Phosphorylation in the C-terminus weakens the interaction with CDC45 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9DD18.
PaxDbiQ9DD18.
PeptideAtlasiQ9DD18.
PRIDEiQ9DD18.

PTM databases

iPTMnetiQ9DD18.
PhosphoSitePlusiQ9DD18.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027430.
GenevisibleiQ9DD18. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with CDC45 and TOPBP1 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9DD18. 1 interactor.
MINTiMINT-4093686.
STRINGi10090.ENSMUSP00000028917.

Structurei

3D structure databases

ProteinModelPortaliQ9DD18.
SMRiQ9DD18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi139 – 140Gly-cisPro motif, important for rejection of L-amino acidsBy similarity2

Domaini

A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.By similarity

Sequence similaritiesi

Belongs to the DTD family.Curated

Phylogenomic databases

eggNOGiKOG3323. Eukaryota.
COG1490. LUCA.
GeneTreeiENSGT00390000008298.
HOVERGENiHBG039436.
InParanoidiQ9DD18.
KOiK07560.
OMAiELAQPFY.
OrthoDBiEOG091G0R8Q.
PhylomeDBiQ9DD18.
TreeFamiTF314886.

Family and domain databases

CDDicd00563. Dtyr_deacylase. 1 hit.
Gene3Di3.50.80.10. 2 hits.
HAMAPiMF_00518. Deacylase_Dtd. 1 hit.
InterProiView protein in InterPro
IPR003732. Daa-tRNA_deacyls_DTD.
IPR023509. DTD-like_dom.
PANTHERiPTHR10472. PTHR10472. 1 hit.
PfamiView protein in Pfam
PF02580. Tyr_Deacylase. 1 hit.
SUPFAMiSSF69500. SSF69500. 1 hit.
TIGRFAMsiTIGR00256. TIGR00256. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9DD18-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKAVVQRVTR ASVTVGGEQI SAIGRGICVL LGISMEDSQK ELEHMVRKIL
60 70 80 90 100
NLRVFEDESG KHWSKSVMDK EYEVLCVSQF TLQCVLKGNK PDFHLAMPTE
110 120 130 140 150
QAESFYNSFL EQLRKSYRPE LIRDGKFGAY MQVHIQNDGP VTIELESPAP
160 170 180 190 200
GAASSDPKQL SKLEKQQQRK EKTRAKGPSE SSKERNAPRK EDRSASSGAE

GDVSSEREP
Length:209
Mass (Da):23,384
Last modified:June 12, 2007 - v2
Checksum:iB6FBB1BB090A060F
GO
Isoform 2 (identifier: Q9DD18-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     199-209: AEGDVSSEREP → DRG

Show »
Length:201
Mass (Da):22,556
Checksum:iA36E116C783352DD
GO

Sequence cautioni

The sequence BAB30808 differs from that shown. Reason: Frameshift at position 199.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30L → F in BAB27205 (PubMed:16141072).Curated1
Sequence conflicti102A → T in BAB30808 (PubMed:16141072).Curated1
Sequence conflicti145L → M in BAE34719 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_026130199 – 209AEGDVSSEREP → DRG in isoform 2. 2 PublicationsAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003594 mRNA. Translation: BAB22882.1.
AK010822 mRNA. Translation: BAB27205.1.
AK011917 mRNA. Translation: BAB27914.1.
AK017565 mRNA. Translation: BAB30808.1. Frameshift.
AK158898 mRNA. Translation: BAE34719.1.
AL844516, AL808119 Genomic DNA. Translation: CAM22364.1.
AL808119, AL844516 Genomic DNA. Translation: CAM26713.1.
BC026537 mRNA. Translation: AAH26537.1.
CCDSiCCDS16824.1. [Q9DD18-1]
RefSeqiNP_079590.1. NM_025314.3. [Q9DD18-1]
UniGeneiMm.28109.

Genome annotation databases

EnsembliENSMUST00000028917; ENSMUSP00000028917; ENSMUSG00000027430. [Q9DD18-1]
GeneIDi66044.
KEGGimmu:66044.
UCSCiuc008mro.2. mouse. [Q9DD18-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDTD1_MOUSE
AccessioniPrimary (citable) accession number: Q9DD18
Secondary accession number(s): A2ANA2
, Q3TY44, Q9CRE8, Q9CYL0, Q9D013, Q9D1G4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: June 12, 2007
Last modified: September 27, 2017
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families