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Protein

D-tyrosyl-tRNA(Tyr) deacylase 1

Gene

Dtd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATPase involved in DNA replication, may facilitate loading of CDC45 onto pre-replication complexes. May hydrolyze D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr), a possible defense mechanism against a harmful effect of D-tyrosine (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi4 – 41Magnesium; via carbonyl oxygenBy similarity
Metal bindingi6 – 61MagnesiumBy similarity
Metal bindingi28 – 281Magnesium; via carbonyl oxygenBy similarity
Active sitei81 – 811NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
D-tyrosyl-tRNA(Tyr) deacylase 1 (EC:3.1.-.-)
Alternative name(s):
DNA-unwinding element-binding protein B
Short name:
DUE-B
Gene namesi
Name:Dtd1
Synonyms:Hars2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1913294. Dtd1.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm Curated

  • Note: Associated with chromatin at some replication origins containing functional DNA-unwinding elements.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209D-tyrosyl-tRNA(Tyr) deacylase 1PRO_0000164627Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei197 – 1971PhosphoserineCombined sources
Modified residuei204 – 2041PhosphoserineCombined sources
Modified residuei205 – 2051PhosphoserineCombined sources

Post-translational modificationi

Preferentially phosphorylated in cells arrested early in S phase. Phosphorylation in the C-terminus weakens the interaction with CDC45 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9DD18.
MaxQBiQ9DD18.
PaxDbiQ9DD18.
PeptideAtlasiQ9DD18.
PRIDEiQ9DD18.

PTM databases

iPTMnetiQ9DD18.
PhosphoSiteiQ9DD18.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027430.
GenevisibleiQ9DD18. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with CDC45 and TOPBP1 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9DD18. 1 interaction.
MINTiMINT-4093686.
STRINGi10090.ENSMUSP00000028917.

Structurei

3D structure databases

ProteinModelPortaliQ9DD18.
SMRiQ9DD18. Positions 1-150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DTD family.Curated

Phylogenomic databases

eggNOGiKOG3323. Eukaryota.
COG1490. LUCA.
GeneTreeiENSGT00390000008298.
HOVERGENiHBG039436.
InParanoidiQ9DD18.
KOiK07560.
OMAiELAQPFY.
OrthoDBiEOG091G0R8Q.
PhylomeDBiQ9DD18.
TreeFamiTF314886.

Family and domain databases

CDDicd00563. Dtyr_deacylase. 1 hit.
Gene3Di3.50.80.10. 1 hit.
HAMAPiMF_00518. Deacylase_Dtd. 1 hit.
InterProiIPR003732. Daa-tRNA_deacyls_DTD.
IPR023509. DTD-like_dom.
[Graphical view]
PANTHERiPTHR10472. PTHR10472. 1 hit.
PfamiPF02580. Tyr_Deacylase. 1 hit.
[Graphical view]
SUPFAMiSSF69500. SSF69500. 1 hit.
TIGRFAMsiTIGR00256. TIGR00256. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9DD18-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKAVVQRVTR ASVTVGGEQI SAIGRGICVL LGISMEDSQK ELEHMVRKIL
60 70 80 90 100
NLRVFEDESG KHWSKSVMDK EYEVLCVSQF TLQCVLKGNK PDFHLAMPTE
110 120 130 140 150
QAESFYNSFL EQLRKSYRPE LIRDGKFGAY MQVHIQNDGP VTIELESPAP
160 170 180 190 200
GAASSDPKQL SKLEKQQQRK EKTRAKGPSE SSKERNAPRK EDRSASSGAE

GDVSSEREP
Length:209
Mass (Da):23,384
Last modified:June 12, 2007 - v2
Checksum:iB6FBB1BB090A060F
GO
Isoform 2 (identifier: Q9DD18-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     199-209: AEGDVSSEREP → DRG

Show »
Length:201
Mass (Da):22,556
Checksum:iA36E116C783352DD
GO

Sequence cautioni

The sequence BAB30808 differs from that shown. Reason: Frameshift at position 199. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301L → F in BAB27205 (PubMed:16141072).Curated
Sequence conflicti102 – 1021A → T in BAB30808 (PubMed:16141072).Curated
Sequence conflicti145 – 1451L → M in BAE34719 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei199 – 20911AEGDVSSEREP → DRG in isoform 2. 2 PublicationsVSP_026130Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003594 mRNA. Translation: BAB22882.1.
AK010822 mRNA. Translation: BAB27205.1.
AK011917 mRNA. Translation: BAB27914.1.
AK017565 mRNA. Translation: BAB30808.1. Frameshift.
AK158898 mRNA. Translation: BAE34719.1.
AL844516, AL808119 Genomic DNA. Translation: CAM22364.1.
AL808119, AL844516 Genomic DNA. Translation: CAM26713.1.
BC026537 mRNA. Translation: AAH26537.1.
CCDSiCCDS16824.1. [Q9DD18-1]
RefSeqiNP_079590.1. NM_025314.3. [Q9DD18-1]
UniGeneiMm.28109.

Genome annotation databases

EnsembliENSMUST00000028917; ENSMUSP00000028917; ENSMUSG00000027430. [Q9DD18-1]
GeneIDi66044.
KEGGimmu:66044.
UCSCiuc008mro.2. mouse. [Q9DD18-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003594 mRNA. Translation: BAB22882.1.
AK010822 mRNA. Translation: BAB27205.1.
AK011917 mRNA. Translation: BAB27914.1.
AK017565 mRNA. Translation: BAB30808.1. Frameshift.
AK158898 mRNA. Translation: BAE34719.1.
AL844516, AL808119 Genomic DNA. Translation: CAM22364.1.
AL808119, AL844516 Genomic DNA. Translation: CAM26713.1.
BC026537 mRNA. Translation: AAH26537.1.
CCDSiCCDS16824.1. [Q9DD18-1]
RefSeqiNP_079590.1. NM_025314.3. [Q9DD18-1]
UniGeneiMm.28109.

3D structure databases

ProteinModelPortaliQ9DD18.
SMRiQ9DD18. Positions 1-150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9DD18. 1 interaction.
MINTiMINT-4093686.
STRINGi10090.ENSMUSP00000028917.

PTM databases

iPTMnetiQ9DD18.
PhosphoSiteiQ9DD18.

Proteomic databases

EPDiQ9DD18.
MaxQBiQ9DD18.
PaxDbiQ9DD18.
PeptideAtlasiQ9DD18.
PRIDEiQ9DD18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028917; ENSMUSP00000028917; ENSMUSG00000027430. [Q9DD18-1]
GeneIDi66044.
KEGGimmu:66044.
UCSCiuc008mro.2. mouse. [Q9DD18-1]

Organism-specific databases

CTDi92675.
MGIiMGI:1913294. Dtd1.

Phylogenomic databases

eggNOGiKOG3323. Eukaryota.
COG1490. LUCA.
GeneTreeiENSGT00390000008298.
HOVERGENiHBG039436.
InParanoidiQ9DD18.
KOiK07560.
OMAiELAQPFY.
OrthoDBiEOG091G0R8Q.
PhylomeDBiQ9DD18.
TreeFamiTF314886.

Miscellaneous databases

ChiTaRSiDtd1. mouse.
PROiQ9DD18.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027430.
GenevisibleiQ9DD18. MM.

Family and domain databases

CDDicd00563. Dtyr_deacylase. 1 hit.
Gene3Di3.50.80.10. 1 hit.
HAMAPiMF_00518. Deacylase_Dtd. 1 hit.
InterProiIPR003732. Daa-tRNA_deacyls_DTD.
IPR023509. DTD-like_dom.
[Graphical view]
PANTHERiPTHR10472. PTHR10472. 1 hit.
PfamiPF02580. Tyr_Deacylase. 1 hit.
[Graphical view]
SUPFAMiSSF69500. SSF69500. 1 hit.
TIGRFAMsiTIGR00256. TIGR00256. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDTD1_MOUSE
AccessioniPrimary (citable) accession number: Q9DD18
Secondary accession number(s): A2ANA2
, Q3TY44, Q9CRE8, Q9CYL0, Q9D013, Q9D1G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: June 12, 2007
Last modified: September 7, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.