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Protein

Ras-related protein Rab-13

Gene

Rab13

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis.1 Publication

Enzyme regulationi

Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP). That Rab may be activated by DENND1C, a guanine exchange factor. Activated in response to insulin (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228GTPBy similarity
Nucleotide bindingi63 – 675GTPBy similarity
Nucleotide bindingi121 – 1244GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-13
Gene namesi
Name:Rab13
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1927232. Rab13.

Subcellular locationi

GO - Cellular componenti

  • bicellular tight junction Source: MGI
  • cell-cell junction Source: MGI
  • cytoplasm Source: MGI
  • cytoplasmic, membrane-bounded vesicle Source: MGI
  • cytoplasmic vesicle Source: UniProtKB
  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • endocytic vesicle Source: UniProtKB
  • extracellular exosome Source: MGI
  • extrinsic component of plasma membrane Source: MGI
  • Golgi apparatus Source: MGI
  • insulin-responsive compartment Source: UniProtKB
  • lamellipodium Source: UniProtKB
  • lateral plasma membrane Source: UniProtKB
  • neuron projection Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • recycling endosome Source: UniProtKB
  • recycling endosome membrane Source: UniProtKB-SubCell
  • trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 199199Ras-related protein Rab-13PRO_0000121183Add
BLAST
Propeptidei200 – 2023Removed in mature formSequence analysisPRO_0000370758

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei178 – 1781PhosphoserineBy similarity
Modified residuei199 – 1991Cysteine methyl esterSequence analysis
Lipidationi199 – 1991S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiQ9DD03.
MaxQBiQ9DD03.
PaxDbiQ9DD03.
PRIDEiQ9DD03.

PTM databases

PhosphoSiteiQ9DD03.

Expressioni

Gene expression databases

BgeeiQ9DD03.
CleanExiMM_RAB13.
ExpressionAtlasiQ9DD03. baseline and differential.
GenevisibleiQ9DD03. MM.

Interactioni

Subunit structurei

Interacts (GTP-bound form) with MICALL2; competes with RAB8A and is involved in tight junctions assembly. Interacts (GTP-bound form) with MICALL1. Interacts with PRKACA; downstream effector of RAB13 involved in tight junction assembly. Interacts with GRB2; may recruit RAB13 to the leading edge of migrating endothelial cells where it can activate RHOA. Interacts (isoprenylated form) with PDE6D; dissociates RAB13 from membranes. Interacts with CCDC64B/BICDR2. Interacts with LEPROT and LEPROTL1.3 Publications

Protein-protein interaction databases

BioGridi212806. 3 interactions.
IntActiQ9DD03. 20 interactions.
MINTiMINT-1340848.
STRINGi10090.ENSMUSP00000070588.

Structurei

3D structure databases

ProteinModelPortaliQ9DD03.
SMRiQ9DD03. Positions 6-172.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi37 – 459Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0078. Eukaryota.
ENOG410XPUI. LUCA.
GeneTreeiENSGT00760000118937.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ9DD03.
KOiK06109.
OMAiCDMEVKR.
PhylomeDBiQ9DD03.
TreeFamiTF314097.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DD03-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKAYDHLFK LLLIGDSGVG KTCLIIRFAE DNFNSTYIST IGIDFKIRTV
60 70 80 90 100
DIEGKRIKLQ VWDTAGQERF KTITTAYYRG AMGIILVYDI TDEKSFENIQ
110 120 130 140 150
NWMKSIKENA SAGVERLLLG NKCDMEAKRQ VQREQAEKLA REHRIRFFET
160 170 180 190 200
SAKSSVNVDE AFSSLARDIL LKTGGRRSGT NSKPSSTGLK TSDKKKNKCL

LG
Length:202
Mass (Da):22,770
Last modified:June 1, 2001 - v1
Checksum:i5DF599432E228AC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002303 mRNA. Translation: BAB22000.1.
BC027214 mRNA. Translation: AAH27214.1.
CCDSiCCDS17522.1.
RefSeqiNP_080953.1. NM_026677.4.
UniGeneiMm.29355.

Genome annotation databases

EnsembliENSMUST00000065418; ENSMUSP00000070588; ENSMUSG00000027935.
GeneIDi68328.
KEGGimmu:68328.
UCSCiuc008qbm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002303 mRNA. Translation: BAB22000.1.
BC027214 mRNA. Translation: AAH27214.1.
CCDSiCCDS17522.1.
RefSeqiNP_080953.1. NM_026677.4.
UniGeneiMm.29355.

3D structure databases

ProteinModelPortaliQ9DD03.
SMRiQ9DD03. Positions 6-172.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212806. 3 interactions.
IntActiQ9DD03. 20 interactions.
MINTiMINT-1340848.
STRINGi10090.ENSMUSP00000070588.

PTM databases

PhosphoSiteiQ9DD03.

Proteomic databases

EPDiQ9DD03.
MaxQBiQ9DD03.
PaxDbiQ9DD03.
PRIDEiQ9DD03.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065418; ENSMUSP00000070588; ENSMUSG00000027935.
GeneIDi68328.
KEGGimmu:68328.
UCSCiuc008qbm.2. mouse.

Organism-specific databases

CTDi5872.
MGIiMGI:1927232. Rab13.

Phylogenomic databases

eggNOGiKOG0078. Eukaryota.
ENOG410XPUI. LUCA.
GeneTreeiENSGT00760000118937.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ9DD03.
KOiK06109.
OMAiCDMEVKR.
PhylomeDBiQ9DD03.
TreeFamiTF314097.

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.

Miscellaneous databases

NextBioi327005.
PROiQ9DD03.
SOURCEiSearch...

Gene expression databases

BgeeiQ9DD03.
CleanExiMM_RAB13.
ExpressionAtlasiQ9DD03. baseline and differential.
GenevisibleiQ9DD03. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin."
    Terai T., Nishimura N., Kanda I., Yasui N., Sasaki T.
    Mol. Biol. Cell 17:2465-2475(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MICALL2.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney and Testis.
  5. "Pericentrosomal targeting of Rab6 secretory vesicles by Bicaudal-D-related protein 1 (BICDR-1) regulates neuritogenesis."
    Schlager M.A., Kapitein L.C., Grigoriev I., Burzynski G.M., Wulf P.S., Keijzer N., de Graaff E., Fukuda M., Shepherd I.T., Akhmanova A., Hoogenraad C.C.
    EMBO J. 29:1637-1651(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CCDC64B.
  6. "Rab13-dependent trafficking of RhoA is required for directional migration and angiogenesis."
    Wu C., Agrawal S., Vasanji A., Drazba J., Sarkaria S., Xie J., Welch C.M., Liu M., Anand-Apte B., Horowitz A.
    J. Biol. Chem. 286:23511-23520(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN ANGIOGENESIS, SUBCELLULAR LOCATION, INTERACTION WITH GRB2.

Entry informationi

Entry nameiRAB13_MOUSE
AccessioniPrimary (citable) accession number: Q9DD03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2001
Last modified: May 11, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.