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Protein

Electron transfer flavoprotein subunit beta

Gene

Etfb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (By similarity). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism (PubMed:25023281). ETFB binds an AMP molecule that probably has a purely structural role (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9AMP; via carbonyl oxygenBy similarity1
Binding sitei66AMP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 42AMPBy similarity4
Nucleotide bindingi123 – 134AMPBy similarityAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • fatty acid beta-oxidation using acyl-CoA dehydrogenase Source: UniProtKB

Keywordsi

Biological processElectron transport, Transport
LigandNucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-611105 Respiratory electron transport
R-MMU-8876725 Protein methylation

Names & Taxonomyi

Protein namesi
Recommended name:
Electron transfer flavoprotein subunit betaCurated
Short name:
Beta-ETFBy similarity
Gene namesi
Name:EtfbImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:106098 Etfb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001678712 – 255Electron transfer flavoprotein subunit betaAdd BLAST254

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei200N6,N6,N6-trimethyllysine; by ETFBKMT; alternate1 Publication1
Modified residuei200N6-acetyllysine; alternateCombined sources1
Modified residuei200N6-methyllysine; alternateBy similarity1
Modified residuei203N6,N6,N6-trimethyllysine; by ETFBKMT1 Publication1
Modified residuei210N6-acetyllysine; alternateCombined sources1
Modified residuei210N6-succinyllysine; alternateCombined sources1
Modified residuei223PhosphoserineBy similarity1
Modified residuei226PhosphoserineBy similarity1
Modified residuei238N6-acetyllysineCombined sources1
Modified residuei248N6-acetyllysine; alternateCombined sources1
Modified residuei248N6-succinyllysine; alternateCombined sources1

Post-translational modificationi

Methylated (PubMed:25023281). Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from acyl-CoA dehydrogenases.By similarity1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9DCW4
MaxQBiQ9DCW4
PaxDbiQ9DCW4
PeptideAtlasiQ9DCW4
PRIDEiQ9DCW4
TopDownProteomicsiQ9DCW4

2D gel databases

REPRODUCTION-2DPAGEiQ9DCW4

PTM databases

iPTMnetiQ9DCW4
PhosphoSitePlusiQ9DCW4
SwissPalmiQ9DCW4

Expressioni

Gene expression databases

BgeeiENSMUSG00000004610
ExpressionAtlasiQ9DCW4 baseline and differential
GenevisibleiQ9DCW4 MM

Interactioni

Subunit structurei

Heterodimer composed of ETFA and ETFB. Identified in a complex that contains ETFA, ETFB and ETFRF1. Interacts with ACADM.By similarity

Protein-protein interaction databases

BioGridi225938, 4 interactors
IntActiQ9DCW4, 6 interactors
MINTiQ9DCW4
STRINGi10090.ENSMUSP00000004729

Structurei

3D structure databases

ProteinModelPortaliQ9DCW4
SMRiQ9DCW4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni183 – 205Recognition loopBy similarityAdd BLAST23

Domaini

The recognition loop recognizes a hydrophobic patch at the surface of interacting dehydrogenases and acts as a static anchor at the interface.By similarity

Sequence similaritiesi

Belongs to the ETF beta-subunit/FixA family.Curated

Phylogenomic databases

eggNOGiKOG3180 Eukaryota
COG2086 LUCA
GeneTreeiENSGT00390000009936
HOGENOMiHOG000247877
HOVERGENiHBG005614
InParanoidiQ9DCW4
KOiK03521
OMAiRIPNMRG
OrthoDBiEOG091G0KHH
PhylomeDBiQ9DCW4
TreeFamiTF314039

Family and domain databases

CDDicd01714 ETF_beta, 1 hit
Gene3Di3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR000049 ET-Flavoprotein_bsu_CS
IPR014730 ETF_a/b_N
IPR012255 ETF_b
IPR033948 ETF_beta_N
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR21294 PTHR21294, 1 hit
PfamiView protein in Pfam
PF01012 ETF, 1 hit
PIRSFiPIRSF000090 Beta-ETF, 1 hit
SMARTiView protein in SMART
SM00893 ETF, 1 hit
PROSITEiView protein in PROSITE
PS01065 ETF_BETA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DCW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELRALVAV KRVIDFAVKI RVKPDKSGVV TDGVKHSMNP FCEIAVEEAV
60 70 80 90 100
RLKEKKLVKE IIAVSCGPSQ CQETIRTALA MGADRGIHVE IPGAQAESLG
110 120 130 140 150
PLQVARVLAK LAEKEKVDLL FLGKQAIDDD CNQTGQMTAG LLDWPQGTFA
160 170 180 190 200
SQVTLEGDKV KVEREIDGGL ETLRLKLPAV VTADLRLNEP RYATLPNIMK
210 220 230 240 250
AKKKKIEVVK AGDLGVDLTS KVSVISVEEP PQRSAGVKVE TTEDLVAKLK

EVGRI
Length:255
Mass (Da):27,623
Last modified:January 23, 2007 - v3
Checksum:i89E7100B327822FF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 4Missing in BAB22076 (PubMed:16141072).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002407 mRNA Translation: BAB22076.1
AK150293 mRNA Translation: BAE29447.1
BC069877 mRNA Translation: AAH69877.1
BC049237 mRNA Translation: AAH49237.1
CCDSiCCDS21171.1
RefSeqiNP_080971.2, NM_026695.3
UniGeneiMm.30200

Genome annotation databases

EnsembliENSMUST00000004729; ENSMUSP00000004729; ENSMUSG00000004610
GeneIDi110826
KEGGimmu:110826
UCSCiuc009gmt.2 mouse

Similar proteinsi

Entry informationi

Entry nameiETFB_MOUSE
AccessioniPrimary (citable) accession number: Q9DCW4
Secondary accession number(s): Q810V3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 139 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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