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Protein

Electron transfer flavoprotein subunit beta

Gene

Etfb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).By similarity1 Publication

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD per dimer.By similarity
  • AMPBy similarityNote: Binds 1 AMP per subunit.By similarity

GO - Molecular functioni

GO - Biological processi

  • fatty acid beta-oxidation using acyl-CoA dehydrogenase Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Electron transfer flavoprotein subunit betaCurated
Short name:
Beta-ETFBy similarity
Gene namesi
Name:EtfbImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:106098. Etfb.

Subcellular locationi

  • Mitochondrion matrix By similarity

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • mitochondrial electron transfer flavoprotein complex Source: MGI
  • mitochondrial matrix Source: UniProtKB
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001678712 – 255Electron transfer flavoprotein subunit betaAdd BLAST254

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei200N6,N6,N6-trimethyllysine; by ETFBKMT; alternate1 Publication1
Modified residuei200N6-acetyllysine; alternateCombined sources1
Modified residuei200N6-methyllysine; alternateBy similarity1
Modified residuei203N6,N6,N6-trimethyllysine; by ETFBKMT1 Publication1
Modified residuei210N6-acetyllysine; alternateCombined sources1
Modified residuei210N6-succinyllysine; alternateCombined sources1
Modified residuei223PhosphoserineBy similarity1
Modified residuei226PhosphoserineBy similarity1
Modified residuei238N6-acetyllysineCombined sources1
Modified residuei248N6-acetyllysine; alternateCombined sources1
Modified residuei248N6-succinyllysine; alternateCombined sources1

Post-translational modificationi

Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases.By similarity1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9DCW4.
MaxQBiQ9DCW4.
PaxDbiQ9DCW4.
PeptideAtlasiQ9DCW4.
PRIDEiQ9DCW4.
TopDownProteomicsiQ9DCW4.

2D gel databases

REPRODUCTION-2DPAGEQ9DCW4.

PTM databases

iPTMnetiQ9DCW4.
PhosphoSitePlusiQ9DCW4.
SwissPalmiQ9DCW4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000004610.
ExpressionAtlasiQ9DCW4. baseline and differential.
GenevisibleiQ9DCW4. MM.

Interactioni

Subunit structurei

Electron transfer flavoprotein is a heterodimer composed of ETFA and ETFB. Interacts with ETFRF1.By similarity

Protein-protein interaction databases

BioGridi225938. 3 interactors.
IntActiQ9DCW4. 6 interactors.
MINTiMINT-1859959.
STRINGi10090.ENSMUSP00000004729.

Structurei

3D structure databases

ProteinModelPortaliQ9DCW4.
SMRiQ9DCW4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni183 – 205Recognition loopBy similarityAdd BLAST23

Domaini

The recognition loop recognizes a hydrophobic patch at the surface of interacting dehydrogenases and acts as a static anchor at the interface.By similarity

Sequence similaritiesi

Belongs to the ETF beta-subunit/FixA family.Curated

Phylogenomic databases

eggNOGiKOG3180. Eukaryota.
COG2086. LUCA.
GeneTreeiENSGT00390000009936.
HOGENOMiHOG000247877.
HOVERGENiHBG005614.
InParanoidiQ9DCW4.
KOiK03521.
OMAiMILDQMD.
OrthoDBiEOG091G0KHH.
PhylomeDBiQ9DCW4.
TreeFamiTF314039.

Family and domain databases

CDDicd01714. ETF_beta. 1 hit.
Gene3Di3.40.50.620. 1 hit.
InterProiIPR000049. ET-Flavoprotein_bsu_CS.
IPR014730. ETF_a/b_N.
IPR012255. ETF_b.
IPR033948. ETF_beta_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR21294. PTHR21294. 1 hit.
PfamiPF01012. ETF. 1 hit.
[Graphical view]
PIRSFiPIRSF000090. Beta-ETF. 1 hit.
SMARTiSM00893. ETF. 1 hit.
[Graphical view]
PROSITEiPS01065. ETF_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DCW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELRALVAV KRVIDFAVKI RVKPDKSGVV TDGVKHSMNP FCEIAVEEAV
60 70 80 90 100
RLKEKKLVKE IIAVSCGPSQ CQETIRTALA MGADRGIHVE IPGAQAESLG
110 120 130 140 150
PLQVARVLAK LAEKEKVDLL FLGKQAIDDD CNQTGQMTAG LLDWPQGTFA
160 170 180 190 200
SQVTLEGDKV KVEREIDGGL ETLRLKLPAV VTADLRLNEP RYATLPNIMK
210 220 230 240 250
AKKKKIEVVK AGDLGVDLTS KVSVISVEEP PQRSAGVKVE TTEDLVAKLK

EVGRI
Length:255
Mass (Da):27,623
Last modified:January 23, 2007 - v3
Checksum:i89E7100B327822FF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 4Missing in BAB22076 (PubMed:16141072).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002407 mRNA. Translation: BAB22076.1.
AK150293 mRNA. Translation: BAE29447.1.
BC069877 mRNA. Translation: AAH69877.1.
BC049237 mRNA. Translation: AAH49237.1.
CCDSiCCDS21171.1.
RefSeqiNP_080971.2. NM_026695.3.
UniGeneiMm.30200.

Genome annotation databases

EnsembliENSMUST00000004729; ENSMUSP00000004729; ENSMUSG00000004610.
GeneIDi110826.
KEGGimmu:110826.
UCSCiuc009gmt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002407 mRNA. Translation: BAB22076.1.
AK150293 mRNA. Translation: BAE29447.1.
BC069877 mRNA. Translation: AAH69877.1.
BC049237 mRNA. Translation: AAH49237.1.
CCDSiCCDS21171.1.
RefSeqiNP_080971.2. NM_026695.3.
UniGeneiMm.30200.

3D structure databases

ProteinModelPortaliQ9DCW4.
SMRiQ9DCW4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi225938. 3 interactors.
IntActiQ9DCW4. 6 interactors.
MINTiMINT-1859959.
STRINGi10090.ENSMUSP00000004729.

PTM databases

iPTMnetiQ9DCW4.
PhosphoSitePlusiQ9DCW4.
SwissPalmiQ9DCW4.

2D gel databases

REPRODUCTION-2DPAGEQ9DCW4.

Proteomic databases

EPDiQ9DCW4.
MaxQBiQ9DCW4.
PaxDbiQ9DCW4.
PeptideAtlasiQ9DCW4.
PRIDEiQ9DCW4.
TopDownProteomicsiQ9DCW4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004729; ENSMUSP00000004729; ENSMUSG00000004610.
GeneIDi110826.
KEGGimmu:110826.
UCSCiuc009gmt.2. mouse.

Organism-specific databases

CTDi2109.
MGIiMGI:106098. Etfb.

Phylogenomic databases

eggNOGiKOG3180. Eukaryota.
COG2086. LUCA.
GeneTreeiENSGT00390000009936.
HOGENOMiHOG000247877.
HOVERGENiHBG005614.
InParanoidiQ9DCW4.
KOiK03521.
OMAiMILDQMD.
OrthoDBiEOG091G0KHH.
PhylomeDBiQ9DCW4.
TreeFamiTF314039.

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.

Miscellaneous databases

ChiTaRSiEtfb. mouse.
PROiQ9DCW4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004610.
ExpressionAtlasiQ9DCW4. baseline and differential.
GenevisibleiQ9DCW4. MM.

Family and domain databases

CDDicd01714. ETF_beta. 1 hit.
Gene3Di3.40.50.620. 1 hit.
InterProiIPR000049. ET-Flavoprotein_bsu_CS.
IPR014730. ETF_a/b_N.
IPR012255. ETF_b.
IPR033948. ETF_beta_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR21294. PTHR21294. 1 hit.
PfamiPF01012. ETF. 1 hit.
[Graphical view]
PIRSFiPIRSF000090. Beta-ETF. 1 hit.
SMARTiSM00893. ETF. 1 hit.
[Graphical view]
PROSITEiPS01065. ETF_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiETFB_MOUSE
AccessioniPrimary (citable) accession number: Q9DCW4
Secondary accession number(s): Q810V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.