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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

Gene

Ndufs3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.
R-MMU-6799198. Complex I biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-30kD
Short name:
CI-30kD
NADH-ubiquinone oxidoreductase 30 kDa subunit
Gene namesi
Name:Ndufs3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1915599. Ndufs3.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: MGI
  • mitochondrial membrane Source: UniProtKB
  • mitochondrial respiratory chain complex I Source: UniProtKB
  • mitochondrion Source: MGI
  • myelin sheath Source: UniProtKB
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35MitochondrionBy similarityAdd BLAST35
ChainiPRO_000001999936 – 263NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrialAdd BLAST228

Proteomic databases

EPDiQ9DCT2.
MaxQBiQ9DCT2.
PaxDbiQ9DCT2.
PeptideAtlasiQ9DCT2.
PRIDEiQ9DCT2.
TopDownProteomicsiQ9DCT2.

2D gel databases

World-2DPAGE0004:Q9DCT2.

PTM databases

iPTMnetiQ9DCT2.
PhosphoSitePlusiQ9DCT2.
SwissPalmiQ9DCT2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000005510.
CleanExiMM_NDUFS3.
GenevisibleiQ9DCT2. MM.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits. Interacts with NDUFAF3.By similarity

Protein-protein interaction databases

BioGridi212816. 3 interactors.
DIPiDIP-32378N.
IntActiQ9DCT2. 6 interactors.
MINTiMINT-4112498.
STRINGi10090.ENSMUSP00000005647.

Structurei

3D structure databases

ProteinModelPortaliQ9DCT2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 30 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1713. Eukaryota.
COG0852. LUCA.
GeneTreeiENSGT00390000017480.
HOGENOMiHOG000009797.
HOVERGENiHBG000450.
InParanoidiQ9DCT2.
KOiK03936.
OMAiAECLPKY.
OrthoDBiEOG091G0AFP.
PhylomeDBiQ9DCT2.
TreeFamiTF314794.

Family and domain databases

HAMAPiMF_01357. NDH1_NuoC. 1 hit.
InterProiIPR010218. NADH_DH_suC.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR020396. NADH_UbQ_OxRdtase_CS.
[Graphical view]
PfamiPF00329. Complex1_30kDa. 1 hit.
[Graphical view]
ProDomiPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01961. NuoC_fam. 1 hit.
PROSITEiPS00542. COMPLEX1_30K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DCT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAAARVWC RGLLGAASVG RGAGRPSVLW QHVRRESAAA DKRPTVRPRS
60 70 80 90 100
DVTHKQLSAF GEYVAEILPK YVQQVQVSCL DELEICIHPD GVIPTLTFLR
110 120 130 140 150
DHTNAQFKSL ADLTAVDVPT RQNRFEIVYN LLSLRFNSRI RVKTYADELT
160 170 180 190 200
PIDSIVSVHI AANWYEREVW DMFGVFFFNH PDLRRILTDY GFEGHPFRKD
210 220 230 240 250
FPLTGYVELR YDDEVKRVVA EPVELAQEFR KFDLNSPWEA FPAYRQPPES
260
LKLEAGDKKP ETK
Length:263
Mass (Da):30,149
Last modified:May 1, 2007 - v2
Checksum:i482F6C4D5E991B7F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20G → D in BAB22149 (PubMed:16141072).Curated1
Sequence conflicti23A → P in AAH27270 (PubMed:15489334).Curated1
Sequence conflicti49R → W in AAH27270 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002501 mRNA. Translation: BAB22149.1.
AK088337 mRNA. Translation: BAC40291.1.
AL672241 Genomic DNA. Translation: CAM15313.1.
BC027270 mRNA. Translation: AAH27270.1.
BC119267 mRNA. Translation: AAI19268.1.
BC119269 mRNA. Translation: AAI19270.1.
CCDSiCCDS16418.1.
RefSeqiNP_080964.1. NM_026688.2.
UniGeneiMm.30113.

Genome annotation databases

EnsembliENSMUST00000005647; ENSMUSP00000005647; ENSMUSG00000005510.
GeneIDi68349.
KEGGimmu:68349.
UCSCiuc008ktr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002501 mRNA. Translation: BAB22149.1.
AK088337 mRNA. Translation: BAC40291.1.
AL672241 Genomic DNA. Translation: CAM15313.1.
BC027270 mRNA. Translation: AAH27270.1.
BC119267 mRNA. Translation: AAI19268.1.
BC119269 mRNA. Translation: AAI19270.1.
CCDSiCCDS16418.1.
RefSeqiNP_080964.1. NM_026688.2.
UniGeneiMm.30113.

3D structure databases

ProteinModelPortaliQ9DCT2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212816. 3 interactors.
DIPiDIP-32378N.
IntActiQ9DCT2. 6 interactors.
MINTiMINT-4112498.
STRINGi10090.ENSMUSP00000005647.

PTM databases

iPTMnetiQ9DCT2.
PhosphoSitePlusiQ9DCT2.
SwissPalmiQ9DCT2.

2D gel databases

World-2DPAGE0004:Q9DCT2.

Proteomic databases

EPDiQ9DCT2.
MaxQBiQ9DCT2.
PaxDbiQ9DCT2.
PeptideAtlasiQ9DCT2.
PRIDEiQ9DCT2.
TopDownProteomicsiQ9DCT2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005647; ENSMUSP00000005647; ENSMUSG00000005510.
GeneIDi68349.
KEGGimmu:68349.
UCSCiuc008ktr.1. mouse.

Organism-specific databases

CTDi4722.
MGIiMGI:1915599. Ndufs3.

Phylogenomic databases

eggNOGiKOG1713. Eukaryota.
COG0852. LUCA.
GeneTreeiENSGT00390000017480.
HOGENOMiHOG000009797.
HOVERGENiHBG000450.
InParanoidiQ9DCT2.
KOiK03936.
OMAiAECLPKY.
OrthoDBiEOG091G0AFP.
PhylomeDBiQ9DCT2.
TreeFamiTF314794.

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.
R-MMU-6799198. Complex I biogenesis.

Miscellaneous databases

PROiQ9DCT2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005510.
CleanExiMM_NDUFS3.
GenevisibleiQ9DCT2. MM.

Family and domain databases

HAMAPiMF_01357. NDH1_NuoC. 1 hit.
InterProiIPR010218. NADH_DH_suC.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR020396. NADH_UbQ_OxRdtase_CS.
[Graphical view]
PfamiPF00329. Complex1_30kDa. 1 hit.
[Graphical view]
ProDomiPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01961. NuoC_fam. 1 hit.
PROSITEiPS00542. COMPLEX1_30K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDUS3_MOUSE
AccessioniPrimary (citable) accession number: Q9DCT2
Secondary accession number(s): Q8BTZ3, Q8R073
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: May 1, 2007
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.