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Protein

1,5-anhydro-D-fructose reductase

Gene

Akr1e2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of 1,5-anhydro-D-fructose (AF) to 1,5-anhydro-D-glucitol. Can also catalyze the reduction of various aldehydes and quinones.

Catalytic activityi

1,5-anhydro-D-glucitol + NADP+ = 1,5-anhydro-D-fructose + NADPH.1 Publication

Kineticsi

  1. KM=1.02 mM for 1,5-anhydro-D-fructose1 Publication
  1. Vmax=0.57 µmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351NADPBy similarity
Active sitei40 – 401Proton donorBy similarity
Sitei69 – 691Lowers pKa of active site TyrBy similarity
Binding sitei102 – 1021SubstrateBy similarity
Binding sitei174 – 1741NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi250 – 2589NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.1.1.292. 3474.
SABIO-RKQ9DCT1.

Names & Taxonomyi

Protein namesi
Recommended name:
1,5-anhydro-D-fructose reductase (EC:1.1.1.263)
Short name:
AF reductase
Alternative name(s):
Aldo-keto reductase family 1 member C-like protein 2
Aldo-keto reductase family 1 member E1
Aldo-keto reductase family 1 member E2
Gene namesi
Name:Akr1e2
Synonyms:Akr1cl2, Akr1e1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1914758. Akr1e1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3013011,5-anhydro-D-fructose reductasePRO_0000124673Add
BLAST

Proteomic databases

EPDiQ9DCT1.
MaxQBiQ9DCT1.
PaxDbiQ9DCT1.
PeptideAtlasiQ9DCT1.
PRIDEiQ9DCT1.

PTM databases

iPTMnetiQ9DCT1.
PhosphoSiteiQ9DCT1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000045410.
CleanExiMM_AKR1E1.
ExpressionAtlasiQ9DCT1. baseline and differential.
GenevisibleiQ9DCT1. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9DCT1. 1 interaction.
MINTiMINT-4087401.
STRINGi10090.ENSMUSP00000089459.

Structurei

3D structure databases

ProteinModelPortaliQ9DCT1.
SMRiQ9DCT1. Positions 4-301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
InParanoidiQ9DCT1.
KOiK13981.
OMAiQDMNDIF.
OrthoDBiEOG091G0D69.
PhylomeDBiQ9DCT1.
TreeFamiTF106492.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DCT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENIPTVGLG TWKASPGEVT DAVKLAINLG YRHFDCAYLY HNESEVGMGI
60 70 80 90 100
SEKIKEGVVK REDLFVVSKL WCTCHKKSLV KTACTNTLEA LNLDYLDLYL
110 120 130 140 150
IHWPMGFKPG EKDIPLDRNG KVIPSHTSFL DTWEAMEDLV FEGLVKNLGV
160 170 180 190 200
SNFNHEQLER LLDKPGLRVR PITNQIECHP YLNQKKLIDF CHKRNVSVTA
210 220 230 240 250
YRPLGGSGGG FHLMDDTVIR KIAKKHGKSP AQILIRFQIQ RNLIVIPKSV
260 270 280 290 300
TPSRIRENIQ VFDFELTEKD MEELLSLDKN LRLATFPTTE NHQDYPFHIE

Y
Length:301
Mass (Da):34,461
Last modified:June 1, 2001 - v1
Checksum:i5633663685EF32CA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti105 – 1051M → I in AAB37274 (Ref. 1) Curated
Sequence conflicti283 – 2831L → F in AAB37274 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68535 mRNA. Translation: AAB37274.1.
AK002507 mRNA. Translation: BAB22152.1.
BC012692 mRNA. Translation: AAH12692.1.
CCDSiCCDS26226.1.
RefSeqiNP_061347.2. NM_018859.2.
UniGeneiMm.251908.

Genome annotation databases

EnsembliENSMUST00000091848; ENSMUSP00000089459; ENSMUSG00000045410.
GeneIDi56043.
KEGGimmu:56043.
UCSCiuc007pjs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68535 mRNA. Translation: AAB37274.1.
AK002507 mRNA. Translation: BAB22152.1.
BC012692 mRNA. Translation: AAH12692.1.
CCDSiCCDS26226.1.
RefSeqiNP_061347.2. NM_018859.2.
UniGeneiMm.251908.

3D structure databases

ProteinModelPortaliQ9DCT1.
SMRiQ9DCT1. Positions 4-301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9DCT1. 1 interaction.
MINTiMINT-4087401.
STRINGi10090.ENSMUSP00000089459.

PTM databases

iPTMnetiQ9DCT1.
PhosphoSiteiQ9DCT1.

Proteomic databases

EPDiQ9DCT1.
MaxQBiQ9DCT1.
PaxDbiQ9DCT1.
PeptideAtlasiQ9DCT1.
PRIDEiQ9DCT1.

Protocols and materials databases

DNASUi56043.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000091848; ENSMUSP00000089459; ENSMUSG00000045410.
GeneIDi56043.
KEGGimmu:56043.
UCSCiuc007pjs.1. mouse.

Organism-specific databases

CTDi56043.
MGIiMGI:1914758. Akr1e1.

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
InParanoidiQ9DCT1.
KOiK13981.
OMAiQDMNDIF.
OrthoDBiEOG091G0D69.
PhylomeDBiQ9DCT1.
TreeFamiTF106492.

Enzyme and pathway databases

BRENDAi1.1.1.292. 3474.
SABIO-RKQ9DCT1.

Miscellaneous databases

PROiQ9DCT1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045410.
CleanExiMM_AKR1E1.
ExpressionAtlasiQ9DCT1. baseline and differential.
GenevisibleiQ9DCT1. MM.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKCL2_MOUSE
AccessioniPrimary (citable) accession number: Q9DCT1
Secondary accession number(s): O09125
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.