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Protein

Enoyl-[acyl-carrier-protein] reductase, mitochondrial

Gene

Mecr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Has a preference for short and medium chain substrates, including trans-2-hexenoyl-CoA (C6), trans-2-decenoyl-CoA (C10), and trans-2-hexadecenoyl-CoA (C16).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NADP+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei94Proton donorBy similarity1
Binding sitei167NADPBy similarity1
Binding sitei368NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi193 – 196NADPBy similarity4
Nucleotide bindingi216 – 218NADPBy similarity3
Nucleotide bindingi285 – 288NADPBy similarity4
Nucleotide bindingi310 – 312NADPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism
LigandNADP

Enzyme and pathway databases

ReactomeiR-MMU-77346 Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase, mitochondrial (EC:1.3.1.104)
Alternative name(s):
2-enoyl thioester reductase
Nuclear receptor-binding factor 1
Short name:
NRBF-1
Gene namesi
Name:Mecr
Synonyms:Nrbf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:1349441 Mecr

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 53MitochondrionSequence analysisAdd BLAST53
ChainiPRO_000000088954 – 373Enoyl-[acyl-carrier-protein] reductase, mitochondrialAdd BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61N6-acetyllysine; alternateCombined sources1
Modified residuei61N6-succinyllysine; alternateCombined sources1
Modified residuei252N6-acetyllysine; alternateCombined sources1
Modified residuei252N6-succinyllysine; alternateCombined sources1
Modified residuei267N6-acetyllysine; alternateCombined sources1
Modified residuei267N6-succinyllysine; alternateCombined sources1
Modified residuei316N6-succinyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9DCS3
MaxQBiQ9DCS3
PaxDbiQ9DCS3
PeptideAtlasiQ9DCS3
PRIDEiQ9DCS3

PTM databases

iPTMnetiQ9DCS3
PhosphoSitePlusiQ9DCS3
SwissPalmiQ9DCS3

Expressioni

Gene expression databases

BgeeiENSMUSG00000028910
CleanExiMM_MECR
ExpressionAtlasiQ9DCS3 baseline and differential
GenevisibleiQ9DCS3 MM

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9DCS3, 2 interactors
MINTiQ9DCS3
STRINGi10090.ENSMUSP00000030742

Structurei

3D structure databases

ProteinModelPortaliQ9DCS3
SMRiQ9DCS3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0025 Eukaryota
COG0604 LUCA
GeneTreeiENSGT00550000074483
HOGENOMiHOG000294683
HOVERGENiHBG052446
InParanoidiQ9DCS3
KOiK07512
OMAiAFRGFWM
OrthoDBiEOG091G0BAE
PhylomeDBiQ9DCS3
TreeFamiTF312886

Family and domain databases

InterProiView protein in InterPro
IPR013149 ADH_C
IPR013154 ADH_N
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
IPR020843 PKS_ER
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SMARTiView protein in SMART
SM00829 PKS_ER, 1 hit
SUPFAMiSSF50129 SSF50129, 1 hit
SSF51735 SSF51735, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DCS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVSQRVTGA RARAPQLAGL LEAWYRHGRT TSSYSALSEP SRVRALVYGN
60 70 80 90 100
HGDPAKVVQL KNLELTAVEG SDVHVRMLAA PINPSDINMI QGNYGLLPKL
110 120 130 140 150
PAVGGNEGVG QVIAVGSSVS ALKPGDWVIP ANAGLGTWRT EAVFSEEALI
160 170 180 190 200
GIPKDIPLQS AATLGVNPCT AYRMLVDFEQ LQPGDSVIQN ASNSGVGQAV
210 220 230 240 250
IQIASALRLK TINVVRDRPD IKKLTDRLKD LGADYVLTEE ELRMPETKTI
260 270 280 290 300
FKDLPLPRLA LNCVGGKSST ELLRHLAPGG TMVTYGGMAK QPVTASVSLL
310 320 330 340 350
IFKDLKLRGF WLSQWKKNHS PDEFKELILT LCNLIRQGRL TAPSCSEVPL
360 370
QGYQQALEAS MKPFVSSKQI LTM
Length:373
Mass (Da):40,343
Last modified:April 26, 2005 - v2
Checksum:iEBA9A61A46386AEE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69E → K in BAB22169 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002533 mRNA Translation: BAB22169.1
BC003864 mRNA Translation: AAH03864.1
CCDSiCCDS18715.1
RefSeqiNP_079573.2, NM_025297.2
UniGeneiMm.192706

Genome annotation databases

EnsembliENSMUST00000030742; ENSMUSP00000030742; ENSMUSG00000028910
GeneIDi26922
KEGGimmu:26922
UCSCiuc008vae.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMECR_MOUSE
AccessioniPrimary (citable) accession number: Q9DCS3
Secondary accession number(s): Q99L39
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: May 23, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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