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Protein

NADH-cytochrome b5 reductase 3

Gene

Cyb5r3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.By similarity

Catalytic activityi

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.

Cofactori

FADBy similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi132 – 147FADBy similarityAdd BLAST16
Nucleotide bindingi171 – 206FADBy similarityAdd BLAST36

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14527
ReactomeiR-MMU-196836 Vitamin C (ascorbate) metabolism
R-MMU-211945 Phase I - Functionalization of compounds
R-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-cytochrome b5 reductase 3 (EC:1.6.2.2)
Short name:
B5R
Short name:
Cytochrome b5 reductase
Alternative name(s):
Diaphorase-1
Cleaved into the following 2 chains:
Gene namesi
Name:Cyb5r3
Synonyms:Dia1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:94893 Cyb5r3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000193982 – 301NADH-cytochrome b5 reductase 3 membrane-bound formAdd BLAST300
ChainiPRO_000001940027 – 301NADH-cytochrome b5 reductase 3 soluble formAdd BLAST275

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei42N6-acetyllysineCombined sources1
Modified residuei43PhosphotyrosineBy similarity1
Modified residuei50N6-acetyllysineCombined sources1
Modified residuei120N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ9DCN2
PeptideAtlasiQ9DCN2
PRIDEiQ9DCN2
TopDownProteomicsiQ9DCN2-1 [Q9DCN2-1]
Q9DCN2-2 [Q9DCN2-2]

PTM databases

iPTMnetiQ9DCN2
PhosphoSitePlusiQ9DCN2
SwissPalmiQ9DCN2

Expressioni

Gene expression databases

BgeeiENSMUSG00000018042
CleanExiMM_CYB5R3
ExpressionAtlasiQ9DCN2 baseline and differential
GenevisibleiQ9DCN2 MM

Interactioni

Subunit structurei

Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MOSC2.By similarity

Protein-protein interaction databases

DIPiDIP-57517N
IntActiQ9DCN2, 8 interactors
MINTiQ9DCN2
STRINGi10090.ENSMUSP00000018186

Structurei

3D structure databases

ProteinModelPortaliQ9DCN2
SMRiQ9DCN2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 152FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0534 Eukaryota
COG0543 LUCA
GeneTreeiENSGT00390000008881
HOGENOMiHOG000175005
HOVERGENiHBG052580
InParanoidiQ9DCN2
KOiK00326
OMAiNGPMSTH
PhylomeDBiQ9DCN2
TreeFamiTF314333

Family and domain databases

InterProiView protein in InterPro
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR001834 NADH-Cyt_B5_reductase
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00175 NAD_binding_1, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00371 FPNCR
SUPFAMiSSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform 1 (identifier: Q9DCN2-1) [UniParc]FASTAAdd to basket
Also known as: M

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAQLSTLSH VVLSPVWFIY SLFMKLFQRS TPAITLENPD IKYPLRLIDK
60 70 80 90 100
EVISPDTRRF RFALPSPQHI LGLPIGQHIY LSTRIDGNLV IRPYTPVSSD
110 120 130 140 150
DDKGFVDLVV KVYFKDTHPK FPAGGKMSQY LENMKIGDTI EFRGPNGLLV
160 170 180 190 200
YQGKGKFAIR ADKKSNPVVR TVKSVGMIAG GTGITPMLQV IRAVLKDPND
210 220 230 240 250
HTVCYLLFAN QSEKDILLRP ELEELRNEHS ARFKLWYTVD KAPDAWDYSQ
260 270 280 290 300
GFVNEEMIRD HLPTPGEEPL ILMCGPPPMI QFACLPNLER VGHPKERCFT

F
Length:301
Mass (Da):34,128
Last modified:January 23, 2007 - v3
Checksum:i984D5B73430F725D
GO
Isoform 2 (identifier: Q9DCN2-2) [UniParc]FASTAAdd to basket
Also known as: S

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.

Show »
Length:278
Mass (Da):31,550
Checksum:iE18DE5668ECD3939
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0129521 – 23Missing in isoform 2. CuratedAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF332059 mRNA Translation: AAK56088.1
AF332060 mRNA Translation: AAK56089.1
AK002640 mRNA Translation: BAB22252.1
BC004760 mRNA Translation: AAH04760.1
BC032013 mRNA Translation: AAH32013.1
BC043074 mRNA Translation: AAH43074.1
CCDSiCCDS27698.1 [Q9DCN2-1]
RefSeqiNP_084063.1, NM_029787.2 [Q9DCN2-1]
XP_006520346.1, XM_006520283.3 [Q9DCN2-2]
UniGeneiMm.22560

Genome annotation databases

EnsembliENSMUST00000018186; ENSMUSP00000018186; ENSMUSG00000018042 [Q9DCN2-1]
GeneIDi109754
KEGGimmu:109754
UCSCiuc007xac.2 mouse [Q9DCN2-1]

Keywords - Coding sequence diversityi

Alternative promoter usage

Similar proteinsi

Entry informationi

Entry nameiNB5R3_MOUSE
AccessioniPrimary (citable) accession number: Q9DCN2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 143 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health