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Protein

NADH-cytochrome b5 reductase 3

Gene

Cyb5r3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.By similarity

Catalytic activityi

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.

Cofactori

FADBy similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi132 – 147FADBy similarityAdd BLAST16
Nucleotide bindingi171 – 206FADBy similarityAdd BLAST36

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14527.
ReactomeiR-MMU-196836. Vitamin C (ascorbate) metabolism.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-cytochrome b5 reductase 3 (EC:1.6.2.2)
Short name:
B5R
Short name:
Cytochrome b5 reductase
Alternative name(s):
Diaphorase-1
Cleaved into the following 2 chains:
Gene namesi
Name:Cyb5r3
Synonyms:Dia1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:94893. Cyb5r3.

Subcellular locationi

Isoform 1 :
  • Endoplasmic reticulum membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
  • Mitochondrion outer membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
Isoform 2 :
  • Cytoplasm

  • Note: Produces the soluble form found in erythrocytes.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000193982 – 301NADH-cytochrome b5 reductase 3 membrane-bound formAdd BLAST300
ChainiPRO_000001940027 – 301NADH-cytochrome b5 reductase 3 soluble formAdd BLAST275

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei42N6-acetyllysineCombined sources1
Modified residuei43PhosphotyrosineBy similarity1
Modified residuei50N6-acetyllysineCombined sources1
Modified residuei120N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ9DCN2.
PeptideAtlasiQ9DCN2.
PRIDEiQ9DCN2.
TopDownProteomicsiQ9DCN2-1. [Q9DCN2-1]
Q9DCN2-2. [Q9DCN2-2]

PTM databases

iPTMnetiQ9DCN2.
PhosphoSitePlusiQ9DCN2.
SwissPalmiQ9DCN2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000018042.
CleanExiMM_CYB5R3.
ExpressionAtlasiQ9DCN2. baseline and differential.
GenevisibleiQ9DCN2. MM.

Interactioni

Subunit structurei

Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MOSC2.By similarity

Protein-protein interaction databases

DIPiDIP-57517N.
IntActiQ9DCN2. 7 interactors.
MINTiMINT-1867235.
STRINGi10090.ENSMUSP00000018186.

Structurei

3D structure databases

ProteinModelPortaliQ9DCN2.
SMRiQ9DCN2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 152FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
COG0543. LUCA.
GeneTreeiENSGT00390000008881.
HOGENOMiHOG000175005.
HOVERGENiHBG052580.
InParanoidiQ9DCN2.
KOiK00326.
OMAiPVWFLYN.
PhylomeDBiQ9DCN2.
TreeFamiTF314333.

Family and domain databases

InterProiIPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR001834. NADH-Cyt_B5_reductase.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00406. CYTB5RDTASE.
PR00371. FPNCR.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform 1 (identifier: Q9DCN2-1) [UniParc]FASTAAdd to basket
Also known as: M

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAQLSTLSH VVLSPVWFIY SLFMKLFQRS TPAITLENPD IKYPLRLIDK
60 70 80 90 100
EVISPDTRRF RFALPSPQHI LGLPIGQHIY LSTRIDGNLV IRPYTPVSSD
110 120 130 140 150
DDKGFVDLVV KVYFKDTHPK FPAGGKMSQY LENMKIGDTI EFRGPNGLLV
160 170 180 190 200
YQGKGKFAIR ADKKSNPVVR TVKSVGMIAG GTGITPMLQV IRAVLKDPND
210 220 230 240 250
HTVCYLLFAN QSEKDILLRP ELEELRNEHS ARFKLWYTVD KAPDAWDYSQ
260 270 280 290 300
GFVNEEMIRD HLPTPGEEPL ILMCGPPPMI QFACLPNLER VGHPKERCFT

F
Length:301
Mass (Da):34,128
Last modified:January 23, 2007 - v3
Checksum:i984D5B73430F725D
GO
Isoform 2 (identifier: Q9DCN2-2) [UniParc]FASTAAdd to basket
Also known as: S

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.

Show »
Length:278
Mass (Da):31,550
Checksum:iE18DE5668ECD3939
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0129521 – 23Missing in isoform 2. CuratedAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF332059 mRNA. Translation: AAK56088.1.
AF332060 mRNA. Translation: AAK56089.1.
AK002640 mRNA. Translation: BAB22252.1.
BC004760 mRNA. Translation: AAH04760.1.
BC032013 mRNA. Translation: AAH32013.1.
BC043074 mRNA. Translation: AAH43074.1.
CCDSiCCDS27698.1. [Q9DCN2-1]
RefSeqiNP_084063.1. NM_029787.2. [Q9DCN2-1]
XP_006520346.1. XM_006520283.3. [Q9DCN2-2]
UniGeneiMm.22560.

Genome annotation databases

EnsembliENSMUST00000018186; ENSMUSP00000018186; ENSMUSG00000018042. [Q9DCN2-1]
GeneIDi109754.
KEGGimmu:109754.
UCSCiuc007xac.2. mouse. [Q9DCN2-1]

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF332059 mRNA. Translation: AAK56088.1.
AF332060 mRNA. Translation: AAK56089.1.
AK002640 mRNA. Translation: BAB22252.1.
BC004760 mRNA. Translation: AAH04760.1.
BC032013 mRNA. Translation: AAH32013.1.
BC043074 mRNA. Translation: AAH43074.1.
CCDSiCCDS27698.1. [Q9DCN2-1]
RefSeqiNP_084063.1. NM_029787.2. [Q9DCN2-1]
XP_006520346.1. XM_006520283.3. [Q9DCN2-2]
UniGeneiMm.22560.

3D structure databases

ProteinModelPortaliQ9DCN2.
SMRiQ9DCN2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-57517N.
IntActiQ9DCN2. 7 interactors.
MINTiMINT-1867235.
STRINGi10090.ENSMUSP00000018186.

PTM databases

iPTMnetiQ9DCN2.
PhosphoSitePlusiQ9DCN2.
SwissPalmiQ9DCN2.

Proteomic databases

PaxDbiQ9DCN2.
PeptideAtlasiQ9DCN2.
PRIDEiQ9DCN2.
TopDownProteomicsiQ9DCN2-1. [Q9DCN2-1]
Q9DCN2-2. [Q9DCN2-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018186; ENSMUSP00000018186; ENSMUSG00000018042. [Q9DCN2-1]
GeneIDi109754.
KEGGimmu:109754.
UCSCiuc007xac.2. mouse. [Q9DCN2-1]

Organism-specific databases

CTDi1727.
MGIiMGI:94893. Cyb5r3.

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
COG0543. LUCA.
GeneTreeiENSGT00390000008881.
HOGENOMiHOG000175005.
HOVERGENiHBG052580.
InParanoidiQ9DCN2.
KOiK00326.
OMAiPVWFLYN.
PhylomeDBiQ9DCN2.
TreeFamiTF314333.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14527.
ReactomeiR-MMU-196836. Vitamin C (ascorbate) metabolism.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiCyb5r3. mouse.
PROiQ9DCN2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018042.
CleanExiMM_CYB5R3.
ExpressionAtlasiQ9DCN2. baseline and differential.
GenevisibleiQ9DCN2. MM.

Family and domain databases

InterProiIPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR001834. NADH-Cyt_B5_reductase.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00406. CYTB5RDTASE.
PR00371. FPNCR.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNB5R3_MOUSE
AccessioniPrimary (citable) accession number: Q9DCN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.