Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Peroxisomal NADH pyrophosphatase NUDT12

Gene

Nudt12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes NAD(P)H to NMNH and AMP (2',5'-ADP), and diadenosine diphosphate to AMP. Has also activity towards NAD(P)+, ADP-ribose and diadenosine triphosphate. May act to regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism in this organelle (By similarity).By similarity

Catalytic activityi

NAD(H) + H2O = AMP + NMN(H).

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi370Magnesium or manganeseBy similarity1
Metal bindingi374Magnesium or manganeseBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • NAD+ diphosphatase activity Source: MGI
  • NADH pyrophosphatase activity Source: MGI

GO - Biological processi

  • NAD catabolic process Source: MGI
  • NADP catabolic process Source: MGI

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Manganese, Metal-binding, NAD, NADP

Enzyme and pathway databases

ReactomeiR-MMU-197264 Nicotinamide salvaging

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal NADH pyrophosphatase NUDT12 (EC:3.6.1.22)
Alternative name(s):
Nucleoside diphosphate-linked moiety X motif 12
Short name:
Nudix motif 12
Gene namesi
Name:Nudt12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1915243 Nudt12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000569571 – 462Peroxisomal NADH pyrophosphatase NUDT12Add BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10N6-succinyllysineCombined sources1
Modified residuei185N6-succinyllysineCombined sources1
Modified residuei292N6-succinyllysineCombined sources1

Proteomic databases

PaxDbiQ9DCN1
PeptideAtlasiQ9DCN1
PRIDEiQ9DCN1

PTM databases

iPTMnetiQ9DCN1
PhosphoSitePlusiQ9DCN1
SwissPalmiQ9DCN1

Expressioni

Gene expression databases

BgeeiENSMUSG00000024228
GenevisibleiQ9DCN1 MM

Interactioni

Protein-protein interaction databases

IntActiQ9DCN1, 1 interactor
MINTiQ9DCN1
STRINGi10090.ENSMUSP00000025065

Structurei

3D structure databases

ProteinModelPortaliQ9DCN1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati11 – 40ANK 1Add BLAST30
Repeati45 – 74ANK 2Add BLAST30
Repeati78 – 98ANK 3Add BLAST21
Domaini319 – 453Nudix hydrolasePROSITE-ProRule annotationAdd BLAST135

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi355 – 376Nudix boxAdd BLAST22
Motifi460 – 462Microbody targeting signalBy similarity3

Sequence similaritiesi

Belongs to the Nudix hydrolase family. NudC subfamily.Curated

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
KOG3084 Eukaryota
COG2816 LUCA
GeneTreeiENSGT00530000063600
HOGENOMiHOG000247937
HOVERGENiHBG054394
InParanoidiQ9DCN1
KOiK03426
OMAiQPEVRLC
OrthoDBiEOG091G0SLC
PhylomeDBiQ9DCN1
TreeFamiTF106352

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR015375 NADH_PPase-like_N
IPR015797 NUDIX_hydrolase-like_dom_sf
IPR020084 NUDIX_hydrolase_CS
IPR000086 NUDIX_hydrolase_dom
IPR015376 Znr_NADH_PPase
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF00293 NUDIX, 1 hit
PF09296 NUDIX-like, 1 hit
PF09297 zf-NADH-PPase, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 3 hits
SUPFAMiSSF48403 SSF48403, 1 hit
SSF55811 SSF55811, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 1 hit
PS51462 NUDIX, 1 hit
PS00893 NUDIX_BOX, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9DCN1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSVKRNPKK EMISELHSSA AEGNVAKLAG ILSHSPSLLN ETSENGWTAL
60 70 80 90 100
MYAARNGHPD VVQFLLEKGC DRSLVNKARQ TALDIAAFWG YRHIANLLAN
110 120 130 140 150
AKGGKKPWFL TNEVDECENY FSRTLLDRRS DKRNNSDWLQ AKESHPTTVY
160 170 180 190 200
LLFSDLNPLV TLGGNKESSQ QPEVRLCQLN YPDVKGYLAQ PEKITLVFLG
210 220 230 240 250
VELEMRKGSP AQAGGVPEEE EDGLVAWFAL GIEPGAAEEF KQRHENCYFL
260 270 280 290 300
HPPMPALLQL KEKEAGVVAQ ARSVLAWHSR YKFCPTCGSA TKIEEGGYKR
310 320 330 340 350
VCVRETCPSL QGVHNTSYPR VDPVVIMQVI HPDGTKCLLG RQKRFPPGMF
360 370 380 390 400
TCLAGFIEPG ETIEDAVRRE VEEESGVKVG HVQYVSCQPW PMPSSLMIGC
410 420 430 440 450
LAVAVSTEIK VDKNEIEDAR WFTREQVVDV LTKGKQQAFF VPPSRAIAHQ
460
LIKHWVGMNP NL
Length:462
Mass (Da):51,511
Last modified:June 1, 2001 - v1
Checksum:i92C2CBB0FAACEA6D
GO
Isoform 2 (identifier: Q9DCN1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     361-367: ETIEDAV → KPILTGF
     368-462: Missing.

Note: No experimental confirmation available.
Show »
Length:367
Mass (Da):40,756
Checksum:i7A0CB8E3A75AA8D1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78A → G in AAH57657 (PubMed:15489334).Curated1
Sequence conflicti290A → V in AAH57657 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014280361 – 367ETIEDAV → KPILTGF in isoform 2. 1 Publication7
Alternative sequenceiVSP_014281368 – 462Missing in isoform 2. 1 PublicationAdd BLAST95

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002641 mRNA Translation: BAB22253.1
BC057657 mRNA Translation: AAH57657.1
CCDSiCCDS28933.1 [Q9DCN1-1]
RefSeqiNP_080773.1, NM_026497.2 [Q9DCN1-1]
UniGeneiMm.36507

Genome annotation databases

EnsembliENSMUST00000025065; ENSMUSP00000025065; ENSMUSG00000024228 [Q9DCN1-1]
ENSMUST00000174122; ENSMUSP00000133678; ENSMUSG00000024228 [Q9DCN1-2]
GeneIDi67993
KEGGimmu:67993
UCSCiuc008dfc.1 mouse [Q9DCN1-1]
uc008dfd.1 mouse [Q9DCN1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNUD12_MOUSE
AccessioniPrimary (citable) accession number: Q9DCN1
Secondary accession number(s): Q6PFA5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health