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Protein

Peroxisomal NADH pyrophosphatase NUDT12

Gene

Nudt12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes NAD(P)H to NMNH and AMP (2',5'-ADP), and diadenosine diphosphate to AMP. Has also activity towards NAD(P)+, ADP-ribose and diadenosine triphosphate. May act to regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism in this organelle (By similarity).By similarity

Catalytic activityi

NAD+ + H2O = AMP + NMN.

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi370 – 3701Magnesium or manganeseBy similarity
Metal bindingi374 – 3741Magnesium or manganeseBy similarity

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • NAD+ diphosphatase activity Source: MGI
  • NADH pyrophosphatase activity Source: MGI

GO - Biological processi

  • NAD catabolic process Source: MGI
  • NADP catabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal NADH pyrophosphatase NUDT12 (EC:3.6.1.22)
Alternative name(s):
Nucleoside diphosphate-linked moiety X motif 12
Short name:
Nudix motif 12
Gene namesi
Name:Nudt12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1915243. Nudt12.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
  • peroxisome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Peroxisomal NADH pyrophosphatase NUDT12PRO_0000056957Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101N6-succinyllysine1 Publication
Modified residuei185 – 1851N6-succinyllysine1 Publication
Modified residuei292 – 2921N6-succinyllysine1 Publication

Proteomic databases

MaxQBiQ9DCN1.
PaxDbiQ9DCN1.
PRIDEiQ9DCN1.

PTM databases

PhosphoSiteiQ9DCN1.

Expressioni

Gene expression databases

BgeeiQ9DCN1.
GenevisibleiQ9DCN1. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9DCN1. 1 interaction.
MINTiMINT-4128687.
STRINGi10090.ENSMUSP00000025065.

Structurei

3D structure databases

ProteinModelPortaliQ9DCN1.
SMRiQ9DCN1. Positions 16-98.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati11 – 4030ANK 1Add
BLAST
Repeati45 – 7430ANK 2Add
BLAST
Repeati78 – 9821ANK 3Add
BLAST
Domaini319 – 453135Nudix hydrolasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi355 – 37622Nudix boxAdd
BLAST
Motifi460 – 4623Microbody targeting signalBy similarity

Sequence similaritiesi

Belongs to the Nudix hydrolase family. NudC subfamily.Curated
Contains 3 ANK repeats.PROSITE-ProRule annotation
Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiCOG2816.
GeneTreeiENSGT00530000063600.
HOGENOMiHOG000247937.
HOVERGENiHBG054394.
InParanoidiQ9DCN1.
KOiK03426.
OMAiEEGGYKR.
OrthoDBiEOG7N0C55.
PhylomeDBiQ9DCN1.
TreeFamiTF106352.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.90.79.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR015375. NADH_PPase-like_N.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
IPR015376. Znr_NADH_PPase.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
PF09296. NUDIX-like. 1 hit.
PF09297. zf-NADH-PPase. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF55811. SSF55811. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9DCN1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSVKRNPKK EMISELHSSA AEGNVAKLAG ILSHSPSLLN ETSENGWTAL
60 70 80 90 100
MYAARNGHPD VVQFLLEKGC DRSLVNKARQ TALDIAAFWG YRHIANLLAN
110 120 130 140 150
AKGGKKPWFL TNEVDECENY FSRTLLDRRS DKRNNSDWLQ AKESHPTTVY
160 170 180 190 200
LLFSDLNPLV TLGGNKESSQ QPEVRLCQLN YPDVKGYLAQ PEKITLVFLG
210 220 230 240 250
VELEMRKGSP AQAGGVPEEE EDGLVAWFAL GIEPGAAEEF KQRHENCYFL
260 270 280 290 300
HPPMPALLQL KEKEAGVVAQ ARSVLAWHSR YKFCPTCGSA TKIEEGGYKR
310 320 330 340 350
VCVRETCPSL QGVHNTSYPR VDPVVIMQVI HPDGTKCLLG RQKRFPPGMF
360 370 380 390 400
TCLAGFIEPG ETIEDAVRRE VEEESGVKVG HVQYVSCQPW PMPSSLMIGC
410 420 430 440 450
LAVAVSTEIK VDKNEIEDAR WFTREQVVDV LTKGKQQAFF VPPSRAIAHQ
460
LIKHWVGMNP NL
Length:462
Mass (Da):51,511
Last modified:June 1, 2001 - v1
Checksum:i92C2CBB0FAACEA6D
GO
Isoform 2 (identifier: Q9DCN1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     361-367: ETIEDAV → KPILTGF
     368-462: Missing.

Note: No experimental confirmation available.
Show »
Length:367
Mass (Da):40,756
Checksum:i7A0CB8E3A75AA8D1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781A → G in AAH57657 (PubMed:15489334).Curated
Sequence conflicti290 – 2901A → V in AAH57657 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei361 – 3677ETIEDAV → KPILTGF in isoform 2. 1 PublicationVSP_014280
Alternative sequencei368 – 46295Missing in isoform 2. 1 PublicationVSP_014281Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002641 mRNA. Translation: BAB22253.1.
BC057657 mRNA. Translation: AAH57657.1.
CCDSiCCDS28933.1. [Q9DCN1-1]
RefSeqiNP_080773.1. NM_026497.2. [Q9DCN1-1]
UniGeneiMm.36507.

Genome annotation databases

EnsembliENSMUST00000025065; ENSMUSP00000025065; ENSMUSG00000024228. [Q9DCN1-1]
ENSMUST00000174122; ENSMUSP00000133678; ENSMUSG00000024228. [Q9DCN1-2]
GeneIDi67993.
KEGGimmu:67993.
UCSCiuc008dfc.1. mouse. [Q9DCN1-1]
uc008dfd.1. mouse. [Q9DCN1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002641 mRNA. Translation: BAB22253.1.
BC057657 mRNA. Translation: AAH57657.1.
CCDSiCCDS28933.1. [Q9DCN1-1]
RefSeqiNP_080773.1. NM_026497.2. [Q9DCN1-1]
UniGeneiMm.36507.

3D structure databases

ProteinModelPortaliQ9DCN1.
SMRiQ9DCN1. Positions 16-98.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9DCN1. 1 interaction.
MINTiMINT-4128687.
STRINGi10090.ENSMUSP00000025065.

PTM databases

PhosphoSiteiQ9DCN1.

Proteomic databases

MaxQBiQ9DCN1.
PaxDbiQ9DCN1.
PRIDEiQ9DCN1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025065; ENSMUSP00000025065; ENSMUSG00000024228. [Q9DCN1-1]
ENSMUST00000174122; ENSMUSP00000133678; ENSMUSG00000024228. [Q9DCN1-2]
GeneIDi67993.
KEGGimmu:67993.
UCSCiuc008dfc.1. mouse. [Q9DCN1-1]
uc008dfd.1. mouse. [Q9DCN1-2]

Organism-specific databases

CTDi83594.
MGIiMGI:1915243. Nudt12.

Phylogenomic databases

eggNOGiCOG2816.
GeneTreeiENSGT00530000063600.
HOGENOMiHOG000247937.
HOVERGENiHBG054394.
InParanoidiQ9DCN1.
KOiK03426.
OMAiEEGGYKR.
OrthoDBiEOG7N0C55.
PhylomeDBiQ9DCN1.
TreeFamiTF106352.

Miscellaneous databases

NextBioi326156.
PROiQ9DCN1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9DCN1.
GenevisibleiQ9DCN1. MM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.90.79.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR015375. NADH_PPase-like_N.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
IPR015376. Znr_NADH_PPase.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
PF09296. NUDIX-like. 1 hit.
PF09297. zf-NADH-PPase. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF55811. SSF55811. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: Czech II.
    Tissue: Mammary tumor.
  3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-10; LYS-185 AND LYS-292, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiNUD12_MOUSE
AccessioniPrimary (citable) accession number: Q9DCN1
Secondary accession number(s): Q6PFA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2001
Last modified: July 22, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.