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Protein

Multifunctional protein ADE2

Gene

Paics

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Multifunctional protein ADE2 (Paics)
  2. Adenylosuccinate lyase (Adsl), Adenylosuccinate lyase (Adsl), Adenylosuccinate lyase (Adsl)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route).
Proteins known to be involved in this subpathway in this organism are:
  1. Multifunctional protein ADE2 (Paics)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei101For SAICAR synthetase activityBy similarity1
Active sitei107For SAICAR synthetase activityBy similarity1
Active sitei215For SAICAR synthetase activityBy similarity1
Active sitei303For AIR carboxylase activityBy similarity1
Active sitei332For AIR carboxylase activityBy similarity1
Sitei334Essential for AIR carboxylase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Ligase, Lyase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00130.
UPA00074; UER00131.

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional protein ADE2
Including the following 2 domains:
Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC:6.3.2.6)
Alternative name(s):
SAICAR synthetase
Phosphoribosylaminoimidazole carboxylase (EC:4.1.1.21)
Alternative name(s):
AIR carboxylase
Short name:
AIRC
Gene namesi
Name:Paics
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1914304. Paics.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000750312 – 425Multifunctional protein ADE2Add BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei22PhosphotyrosineCombined sources1
Modified residuei36N6-acetyllysineCombined sources1
Modified residuei107PhosphoserineBy similarity1
Modified residuei238PhosphothreonineBy similarity1
Modified residuei247N6-acetyllysineBy similarity1
Modified residuei274PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9DCL9.
MaxQBiQ9DCL9.
PaxDbiQ9DCL9.
PeptideAtlasiQ9DCL9.
PRIDEiQ9DCL9.

PTM databases

iPTMnetiQ9DCL9.
PhosphoSitePlusiQ9DCL9.
SwissPalmiQ9DCL9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029247.
ExpressionAtlasiQ9DCL9. baseline and differential.
GenevisibleiQ9DCL9. MM.

Interactioni

Subunit structurei

Homooctamer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211906. 4 interactors.
IntActiQ9DCL9. 3 interactors.
MINTiMINT-1866409.
STRINGi10090.ENSMUSP00000031160.

Structurei

3D structure databases

ProteinModelPortaliQ9DCL9.
SMRiQ9DCL9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 260SAICAR synthetaseAdd BLAST259
Regioni261 – 425AIR carboxylaseAdd BLAST165
Regioni261 – 266LinkerBy similarity6
Regioni267 – 425AIR carboxylaseBy similarityAdd BLAST159

Sequence similaritiesi

In the N-terminal section; belongs to the SAICAR synthetase family.Curated
In the C-terminal section; belongs to the AIR carboxylase family. Class II subfamily.Curated

Phylogenomic databases

eggNOGiKOG2835. Eukaryota.
COG0041. LUCA.
COG0152. LUCA.
GeneTreeiENSGT00390000010172.
HOGENOMiHOG000082628.
HOVERGENiHBG008335.
InParanoidiQ9DCL9.
KOiK01587.
OMAiWQTKNCA.
OrthoDBiEOG091G08DN.
TreeFamiTF106384.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
3.40.50.7700. 1 hit.
HAMAPiMF_02045. PurE_classII. 1 hit.
MF_00137. SAICAR_synth. 1 hit.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR033626. PurE_classII.
IPR000031. PurE_dom.
IPR028923. SAICAR_synt/ADE2_N.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamiPF00731. AIRC. 1 hit.
PF01259. SAICAR_synt. 1 hit.
[Graphical view]
SMARTiSM01001. AIRC. 1 hit.
[Graphical view]
SUPFAMiSSF52255. SSF52255. 1 hit.
PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DCL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAVVVNIG KKLYEGKTKE VYELLDTPGR VLLQSKDQIT AGNAARKNHL
60 70 80 90 100
EGKAAISNKI TSCIFQLLQE AGIKTAFTKK CGETAFIAPQ CEMIPIEWVC
110 120 130 140 150
RRIATGSFLK RNPGVQEGYK FYPPKVEMFF KDDANNDPQW SEEQLIAAKF
160 170 180 190 200
CFAGLVIGQT EVDIMSHATQ AIFEILEKSW LPQDCTLVDM KIEFGVDVTT
210 220 230 240 250
KEIVLADVID NDSWRLWPSG DRSQQKDKQS YRDLKEVTPE GLQMVKKNFE
260 270 280 290 300
WVADRVELLL KSDSQCRVVV LMGSTSDLGH CEKIKKACGN FGIPCELRVT
310 320 330 340 350
SAHKGPDETL RIKAEYEGDG IPTVFVSVAG RSNGLGPVLS GNTAYPVISC
360 370 380 390 400
PPITPDWGAQ DVWSSLRLPS GIGCSTILSP EGSAQFAAQI FGLNNHLVWA
410 420
KLRASILNTW ISLKQADKKV RQCNL
Length:425
Mass (Da):47,006
Last modified:July 27, 2011 - v4
Checksum:iCD2A4323EC02B218
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5V → Y in BAB22273 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002669 mRNA. Translation: BAB22273.1.
AK010462 mRNA. Translation: BAB26957.1.
AK012118 mRNA. Translation: BAB28044.1.
AK146722 mRNA. Translation: BAE27385.1.
AK159398 mRNA. Translation: BAE35051.1.
AK166264 mRNA. Translation: BAE38669.1.
CH466524 Genomic DNA. Translation: EDL37918.1.
BC018153 mRNA. Translation: AAH18153.1.
BC052691 mRNA. Translation: AAH52691.1.
CCDSiCCDS19367.1.
RefSeqiNP_080215.1. NM_025939.2.
XP_006535243.1. XM_006535180.2.
XP_006535244.1. XM_006535181.3.
XP_006535245.1. XM_006535182.3.
XP_006535246.1. XM_006535183.3.
UniGeneiMm.182931.
Mm.431705.

Genome annotation databases

EnsembliENSMUST00000031160; ENSMUSP00000031160; ENSMUSG00000029247.
ENSMUST00000117536; ENSMUSP00000112879; ENSMUSG00000029247.
ENSMUST00000120912; ENSMUSP00000113483; ENSMUSG00000029247.
GeneIDi67054.
KEGGimmu:67054.
UCSCiuc008xvn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002669 mRNA. Translation: BAB22273.1.
AK010462 mRNA. Translation: BAB26957.1.
AK012118 mRNA. Translation: BAB28044.1.
AK146722 mRNA. Translation: BAE27385.1.
AK159398 mRNA. Translation: BAE35051.1.
AK166264 mRNA. Translation: BAE38669.1.
CH466524 Genomic DNA. Translation: EDL37918.1.
BC018153 mRNA. Translation: AAH18153.1.
BC052691 mRNA. Translation: AAH52691.1.
CCDSiCCDS19367.1.
RefSeqiNP_080215.1. NM_025939.2.
XP_006535243.1. XM_006535180.2.
XP_006535244.1. XM_006535181.3.
XP_006535245.1. XM_006535182.3.
XP_006535246.1. XM_006535183.3.
UniGeneiMm.182931.
Mm.431705.

3D structure databases

ProteinModelPortaliQ9DCL9.
SMRiQ9DCL9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211906. 4 interactors.
IntActiQ9DCL9. 3 interactors.
MINTiMINT-1866409.
STRINGi10090.ENSMUSP00000031160.

PTM databases

iPTMnetiQ9DCL9.
PhosphoSitePlusiQ9DCL9.
SwissPalmiQ9DCL9.

Proteomic databases

EPDiQ9DCL9.
MaxQBiQ9DCL9.
PaxDbiQ9DCL9.
PeptideAtlasiQ9DCL9.
PRIDEiQ9DCL9.

Protocols and materials databases

DNASUi67054.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031160; ENSMUSP00000031160; ENSMUSG00000029247.
ENSMUST00000117536; ENSMUSP00000112879; ENSMUSG00000029247.
ENSMUST00000120912; ENSMUSP00000113483; ENSMUSG00000029247.
GeneIDi67054.
KEGGimmu:67054.
UCSCiuc008xvn.2. mouse.

Organism-specific databases

CTDi10606.
MGIiMGI:1914304. Paics.

Phylogenomic databases

eggNOGiKOG2835. Eukaryota.
COG0041. LUCA.
COG0152. LUCA.
GeneTreeiENSGT00390000010172.
HOGENOMiHOG000082628.
HOVERGENiHBG008335.
InParanoidiQ9DCL9.
KOiK01587.
OMAiWQTKNCA.
OrthoDBiEOG091G08DN.
TreeFamiTF106384.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00130.
UPA00074; UER00131.
ReactomeiR-MMU-73817. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

PROiQ9DCL9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029247.
ExpressionAtlasiQ9DCL9. baseline and differential.
GenevisibleiQ9DCL9. MM.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
3.40.50.7700. 1 hit.
HAMAPiMF_02045. PurE_classII. 1 hit.
MF_00137. SAICAR_synth. 1 hit.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR033626. PurE_classII.
IPR000031. PurE_dom.
IPR028923. SAICAR_synt/ADE2_N.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamiPF00731. AIRC. 1 hit.
PF01259. SAICAR_synt. 1 hit.
[Graphical view]
SMARTiSM01001. AIRC. 1 hit.
[Graphical view]
SUPFAMiSSF52255. SSF52255. 1 hit.
PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUR6_MOUSE
AccessioniPrimary (citable) accession number: Q9DCL9
Secondary accession number(s): Q9CQ38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 130 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.