ID   TSN4_MOUSE              Reviewed;         238 AA.
AC   Q9DCK3;
DT   11-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   24-JAN-2024, entry version 151.
DE   RecName: Full=Tetraspanin-4;
DE            Short=Tspan-4;
DE   AltName: Full=Transmembrane 4 superfamily member 7;
GN   Name=Tspan4; Synonyms=Tm4sf7;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Kidney;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Olfactory epithelium;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Kidney, Liver, and Lung;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- SUBUNIT: Forms a complex with integrins. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
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DR   EMBL; AK002709; BAB22301.1; -; mRNA.
DR   EMBL; BC003482; AAH03482.1; -; mRNA.
DR   EMBL; BC083070; AAH83070.1; -; mRNA.
DR   CCDS; CCDS22018.1; -.
DR   RefSeq; NP_001239517.1; NM_001252588.1.
DR   RefSeq; NP_444312.1; NM_053082.3.
DR   AlphaFoldDB; Q9DCK3; -.
DR   SMR; Q9DCK3; -.
DR   IntAct; Q9DCK3; 1.
DR   MINT; Q9DCK3; -.
DR   STRING; 10090.ENSMUSP00000026585; -.
DR   GlyCosmos; Q9DCK3; 2 sites, No reported glycans.
DR   GlyGen; Q9DCK3; 2 sites.
DR   PhosphoSitePlus; Q9DCK3; -.
DR   SwissPalm; Q9DCK3; -.
DR   jPOST; Q9DCK3; -.
DR   MaxQB; Q9DCK3; -.
DR   PaxDb; 10090-ENSMUSP00000026585; -.
DR   ProteomicsDB; 297724; -.
DR   Antibodypedia; 10103; 95 antibodies from 19 providers.
DR   DNASU; 64540; -.
DR   Ensembl; ENSMUST00000026585.14; ENSMUSP00000026585.8; ENSMUSG00000025511.16.
DR   Ensembl; ENSMUST00000117634.2; ENSMUSP00000113085.2; ENSMUSG00000025511.16.
DR   GeneID; 64540; -.
DR   KEGG; mmu:64540; -.
DR   UCSC; uc009kln.2; mouse.
DR   AGR; MGI:1928097; -.
DR   CTD; 7106; -.
DR   MGI; MGI:1928097; Tspan4.
DR   VEuPathDB; HostDB:ENSMUSG00000025511; -.
DR   eggNOG; KOG3882; Eukaryota.
DR   GeneTree; ENSGT00940000160434; -.
DR   HOGENOM; CLU_055524_4_3_1; -.
DR   InParanoid; Q9DCK3; -.
DR   OMA; KADTYCT; -.
DR   OrthoDB; 3234802at2759; -.
DR   PhylomeDB; Q9DCK3; -.
DR   TreeFam; TF352892; -.
DR   BioGRID-ORCS; 64540; 1 hit in 78 CRISPR screens.
DR   ChiTaRS; Tspan4; mouse.
DR   PRO; PR:Q9DCK3; -.
DR   Proteomes; UP000000589; Chromosome 7.
DR   RNAct; Q9DCK3; Protein.
DR   Bgee; ENSMUSG00000025511; Expressed in stroma of bone marrow and 260 other cell types or tissues.
DR   ExpressionAtlas; Q9DCK3; baseline and differential.
DR   GO; GO:0140494; C:migrasome; IDA:MGI.
DR   GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR   GO; GO:0031982; C:vesicle; ISO:MGI.
DR   GO; GO:0003823; F:antigen binding; ISO:MGI.
DR   GO; GO:0005178; F:integrin binding; ISO:MGI.
DR   GO; GO:0160040; P:mitocytosis; IDA:MGI.
DR   GO; GO:0065003; P:protein-containing complex assembly; ISO:MGI.
DR   GO; GO:0051881; P:regulation of mitochondrial membrane potential; IDA:MGI.
DR   CDD; cd03165; NET-5_like_LEL; 1.
DR   Gene3D; 1.10.1450.10; Tetraspanin; 1.
DR   InterPro; IPR018499; Tetraspanin/Peripherin.
DR   InterPro; IPR000301; Tetraspanin_animals.
DR   InterPro; IPR018503; Tetraspanin_CS.
DR   InterPro; IPR008952; Tetraspanin_EC2_sf.
DR   PANTHER; PTHR19282; TETRASPANIN; 1.
DR   PANTHER; PTHR19282:SF40; TETRASPANIN-4; 1.
DR   Pfam; PF00335; Tetraspanin; 1.
DR   PIRSF; PIRSF002419; Tetraspanin; 1.
DR   PRINTS; PR00259; TMFOUR.
DR   SUPFAM; SSF48652; Tetraspanin; 1.
DR   PROSITE; PS00421; TM4_1; 1.
DR   Genevisible; Q9DCK3; MM.
PE   1: Evidence at protein level;
KW   Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..238
FT                   /note="Tetraspanin-4"
FT                   /id="PRO_0000219242"
FT   TOPO_DOM        1..13
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        35..55
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        77..85
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        107..201
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        202..222
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        223..238
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        152
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        161
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   238 AA;  26054 MW;  AA916EF6078777FA CRC64;
     MARGCLQGVK YLMFAFNLLF WLGGCGVLGV GIWLAATQGN FATLSSSFPS LSAANLLIVT
     GTFVMAIGFV GCIGALKENK CLLLTFFVLL LLVFLLEATI AVLFFAYSDK IDSYAQQDLK
     KGLHLYGTQG NVGLTNAWSI IQTDFRCCGV SNYTDWFEVY NATRVPDSCC LEFSDSCGLH
     EPGTWWKSPC YETVKAWLQE NLLAVGIFGL CTALVQILGL TFAMTMYCQV VKADTYCA
//