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Q9DCJ9

- NPL_MOUSE

UniProt

Q9DCJ9 - NPL_MOUSE

Protein

N-acetylneuraminate lyase

Gene

Npl

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway.1 Publication

    Catalytic activityi

    N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei143 – 1431Involved in proton transfer during cleavageBy similarity
    Active sitei173 – 1731Schiff-base intermediate with substrateBy similarity

    GO - Molecular functioni

    1. N-acetylneuraminate lyase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW
    2. N-acetylneuraminate catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    ReactomeiREACT_202500. Sialic acid metabolism.
    UniPathwayiUPA00629.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetylneuraminate lyase (EC:4.1.3.3)
    Short name:
    NALase
    Alternative name(s):
    N-acetylneuraminate pyruvate-lyase
    N-acetylneuraminic acid aldolase
    Sialate lyase
    Sialate-pyruvate lyase
    Sialic acid aldolase
    Sialic acid lyase
    Gene namesi
    Name:Npl
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1921341. Npl.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 320320N-acetylneuraminate lyasePRO_0000273353Add
    BLAST

    Proteomic databases

    MaxQBiQ9DCJ9.
    PaxDbiQ9DCJ9.
    PRIDEiQ9DCJ9.

    PTM databases

    PhosphoSiteiQ9DCJ9.

    Expressioni

    Gene expression databases

    BgeeiQ9DCJ9.
    CleanExiMM_NPL.
    GenevestigatoriQ9DCJ9.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    IntActiQ9DCJ9. 1 interaction.
    MINTiMINT-4128676.
    STRINGi10090.ENSMUSP00000037454.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9DCJ9.
    SMRiQ9DCJ9. Positions 8-280.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni51 – 522Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the DapA family. NanA subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0329.
    GeneTreeiENSGT00530000063604.
    HOGENOMiHOG000218206.
    HOVERGENiHBG082055.
    InParanoidiQ9DCJ9.
    KOiK01639.
    OMAiLKPWNKD.
    OrthoDBiEOG7NKKKP.
    PhylomeDBiQ9DCJ9.
    TreeFamiTF353639.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR002220. DapA-like.
    [Graphical view]
    PANTHERiPTHR12128. PTHR12128. 1 hit.
    PfamiPF00701. DHDPS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001365. DHDPS. 1 hit.
    PRINTSiPR00146. DHPICSNTHASE.

    Sequencei

    Sequence statusi: Complete.

    Q9DCJ9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFPKKKLRG LVAATITPMT ENGEINFPVI GQYVDYLVKE QGVKNIFVNG    50
    TTGEGLSLSV SERRQVAEEW VNQGRNKLDQ VVIHVGALNV KESQELAQHA 100
    AEIGADGIAV IAPFFFKSQN KDALISFLRE VAAAAPTLPF YYYHMPSMTG 150
    VKIRAEELLD GIQDKIPTFQ GLKFTDTDLL DFGQCVDQNH QRQFALLFGV 200
    DEQLLSALVM GATGAVGSTY NYLGKKTNQM LEAFEQKDLA SALSYQFRIQ 250
    RFINYVIKLG FGVSQTKAIM TLVSGIPMGP PRLPLQKATQ EFTAKAEAKL 300
    KSLDFLSSPS VKEGKPLASA 320
    Length:320
    Mass (Da):35,130
    Last modified:June 1, 2001 - v1
    Checksum:i9426AD7CC8438468
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK002734 mRNA. Translation: BAB22314.1.
    AK088859 mRNA. Translation: BAC40618.1.
    BC022734 mRNA. Translation: AAH22734.1.
    CCDSiCCDS15374.1.
    RefSeqiNP_083025.1. NM_028749.1.
    UniGeneiMm.24887.

    Genome annotation databases

    EnsembliENSMUST00000041874; ENSMUSP00000037454; ENSMUSG00000042684.
    GeneIDi74091.
    KEGGimmu:74091.
    UCSCiuc007dac.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK002734 mRNA. Translation: BAB22314.1 .
    AK088859 mRNA. Translation: BAC40618.1 .
    BC022734 mRNA. Translation: AAH22734.1 .
    CCDSi CCDS15374.1.
    RefSeqi NP_083025.1. NM_028749.1.
    UniGenei Mm.24887.

    3D structure databases

    ProteinModelPortali Q9DCJ9.
    SMRi Q9DCJ9. Positions 8-280.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9DCJ9. 1 interaction.
    MINTi MINT-4128676.
    STRINGi 10090.ENSMUSP00000037454.

    PTM databases

    PhosphoSitei Q9DCJ9.

    Proteomic databases

    MaxQBi Q9DCJ9.
    PaxDbi Q9DCJ9.
    PRIDEi Q9DCJ9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000041874 ; ENSMUSP00000037454 ; ENSMUSG00000042684 .
    GeneIDi 74091.
    KEGGi mmu:74091.
    UCSCi uc007dac.1. mouse.

    Organism-specific databases

    CTDi 80896.
    MGIi MGI:1921341. Npl.

    Phylogenomic databases

    eggNOGi COG0329.
    GeneTreei ENSGT00530000063604.
    HOGENOMi HOG000218206.
    HOVERGENi HBG082055.
    InParanoidi Q9DCJ9.
    KOi K01639.
    OMAi LKPWNKD.
    OrthoDBi EOG7NKKKP.
    PhylomeDBi Q9DCJ9.
    TreeFami TF353639.

    Enzyme and pathway databases

    UniPathwayi UPA00629 .
    Reactomei REACT_202500. Sialic acid metabolism.

    Miscellaneous databases

    NextBioi 339741.
    PROi Q9DCJ9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9DCJ9.
    CleanExi MM_NPL.
    Genevestigatori Q9DCJ9.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR002220. DapA-like.
    [Graphical view ]
    PANTHERi PTHR12128. PTHR12128. 1 hit.
    Pfami PF00701. DHDPS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001365. DHDPS. 1 hit.
    PRINTSi PR00146. DHPICSNTHASE.
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Kidney and Thymus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    3. "Metabolism of vertebrate amino sugars with N-glycolyl groups: elucidating the intracellular fate of the non-human sialic acid N-glycolylneuraminic acid."
      Bergfeld A.K., Pearce O.M., Diaz S.L., Pham T., Varki A.
      J. Biol. Chem. 287:28865-28881(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY.

    Entry informationi

    Entry nameiNPL_MOUSE
    AccessioniPrimary (citable) accession number: Q9DCJ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3