SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9DCJ9

- NPL_MOUSE

UniProt

Q9DCJ9 - NPL_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
N-acetylneuraminate lyase
Gene
Npl
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway.1 Publication

Catalytic activityi

N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei143 – 1431Involved in proton transfer during cleavage By similarity
Active sitei173 – 1731Schiff-base intermediate with substrate By similarity

GO - Molecular functioni

  1. N-acetylneuraminate lyase activity Source: UniProtKB

GO - Biological processi

  1. N-acetylneuraminate catabolic process Source: UniProtKB
  2. carbohydrate metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

ReactomeiREACT_202500. Sialic acid metabolism.
UniPathwayiUPA00629.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylneuraminate lyase (EC:4.1.3.3)
Short name:
NALase
Alternative name(s):
N-acetylneuraminate pyruvate-lyase
N-acetylneuraminic acid aldolase
Sialate lyase
Sialate-pyruvate lyase
Sialic acid aldolase
Sialic acid lyase
Gene namesi
Name:Npl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1921341. Npl.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320N-acetylneuraminate lyase
PRO_0000273353Add
BLAST

Proteomic databases

MaxQBiQ9DCJ9.
PaxDbiQ9DCJ9.
PRIDEiQ9DCJ9.

PTM databases

PhosphoSiteiQ9DCJ9.

Expressioni

Gene expression databases

BgeeiQ9DCJ9.
CleanExiMM_NPL.
GenevestigatoriQ9DCJ9.

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

IntActiQ9DCJ9. 1 interaction.
MINTiMINT-4128676.
STRINGi10090.ENSMUSP00000037454.

Structurei

3D structure databases

ProteinModelPortaliQ9DCJ9.
SMRiQ9DCJ9. Positions 8-280.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 522Substrate binding By similarity

Sequence similaritiesi

Belongs to the DapA family. NanA subfamily.

Phylogenomic databases

eggNOGiCOG0329.
GeneTreeiENSGT00530000063604.
HOGENOMiHOG000218206.
HOVERGENiHBG082055.
InParanoidiQ9DCJ9.
KOiK01639.
OMAiLKPWNKD.
OrthoDBiEOG7NKKKP.
PhylomeDBiQ9DCJ9.
TreeFamiTF353639.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.

Sequencei

Sequence statusi: Complete.

Q9DCJ9-1 [UniParc]FASTAAdd to Basket

« Hide

MAFPKKKLRG LVAATITPMT ENGEINFPVI GQYVDYLVKE QGVKNIFVNG    50
TTGEGLSLSV SERRQVAEEW VNQGRNKLDQ VVIHVGALNV KESQELAQHA 100
AEIGADGIAV IAPFFFKSQN KDALISFLRE VAAAAPTLPF YYYHMPSMTG 150
VKIRAEELLD GIQDKIPTFQ GLKFTDTDLL DFGQCVDQNH QRQFALLFGV 200
DEQLLSALVM GATGAVGSTY NYLGKKTNQM LEAFEQKDLA SALSYQFRIQ 250
RFINYVIKLG FGVSQTKAIM TLVSGIPMGP PRLPLQKATQ EFTAKAEAKL 300
KSLDFLSSPS VKEGKPLASA 320
Length:320
Mass (Da):35,130
Last modified:June 1, 2001 - v1
Checksum:i9426AD7CC8438468
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK002734 mRNA. Translation: BAB22314.1.
AK088859 mRNA. Translation: BAC40618.1.
BC022734 mRNA. Translation: AAH22734.1.
CCDSiCCDS15374.1.
RefSeqiNP_083025.1. NM_028749.1.
UniGeneiMm.24887.

Genome annotation databases

EnsembliENSMUST00000041874; ENSMUSP00000037454; ENSMUSG00000042684.
GeneIDi74091.
KEGGimmu:74091.
UCSCiuc007dac.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK002734 mRNA. Translation: BAB22314.1 .
AK088859 mRNA. Translation: BAC40618.1 .
BC022734 mRNA. Translation: AAH22734.1 .
CCDSi CCDS15374.1.
RefSeqi NP_083025.1. NM_028749.1.
UniGenei Mm.24887.

3D structure databases

ProteinModelPortali Q9DCJ9.
SMRi Q9DCJ9. Positions 8-280.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9DCJ9. 1 interaction.
MINTi MINT-4128676.
STRINGi 10090.ENSMUSP00000037454.

PTM databases

PhosphoSitei Q9DCJ9.

Proteomic databases

MaxQBi Q9DCJ9.
PaxDbi Q9DCJ9.
PRIDEi Q9DCJ9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000041874 ; ENSMUSP00000037454 ; ENSMUSG00000042684 .
GeneIDi 74091.
KEGGi mmu:74091.
UCSCi uc007dac.1. mouse.

Organism-specific databases

CTDi 80896.
MGIi MGI:1921341. Npl.

Phylogenomic databases

eggNOGi COG0329.
GeneTreei ENSGT00530000063604.
HOGENOMi HOG000218206.
HOVERGENi HBG082055.
InParanoidi Q9DCJ9.
KOi K01639.
OMAi LKPWNKD.
OrthoDBi EOG7NKKKP.
PhylomeDBi Q9DCJ9.
TreeFami TF353639.

Enzyme and pathway databases

UniPathwayi UPA00629 .
Reactomei REACT_202500. Sialic acid metabolism.

Miscellaneous databases

NextBioi 339741.
PROi Q9DCJ9.
SOURCEi Search...

Gene expression databases

Bgeei Q9DCJ9.
CleanExi MM_NPL.
Genevestigatori Q9DCJ9.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR002220. DapA-like.
[Graphical view ]
PANTHERi PTHR12128. PTHR12128. 1 hit.
Pfami PF00701. DHDPS. 1 hit.
[Graphical view ]
PIRSFi PIRSF001365. DHDPS. 1 hit.
PRINTSi PR00146. DHPICSNTHASE.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Kidney and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  3. "Metabolism of vertebrate amino sugars with N-glycolyl groups: elucidating the intracellular fate of the non-human sialic acid N-glycolylneuraminic acid."
    Bergfeld A.K., Pearce O.M., Diaz S.L., Pham T., Varki A.
    J. Biol. Chem. 287:28865-28881(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY.

Entry informationi

Entry nameiNPL_MOUSE
AccessioniPrimary (citable) accession number: Q9DCJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi