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Protein

N-acetylneuraminate lyase

Gene

Npl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway.1 Publication

Catalytic activityi

N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei143 – 1431Involved in proton transfer during cleavageBy similarity
Active sitei173 – 1731Schiff-base intermediate with substrateBy similarity

GO - Molecular functioni

  1. N-acetylneuraminate lyase activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
  2. N-acetylneuraminate catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

ReactomeiREACT_202500. Sialic acid metabolism.
UniPathwayiUPA00629.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylneuraminate lyase (EC:4.1.3.3)
Short name:
NALase
Alternative name(s):
N-acetylneuraminate pyruvate-lyase
N-acetylneuraminic acid aldolase
Sialate lyase
Sialate-pyruvate lyase
Sialic acid aldolase
Sialic acid lyase
Gene namesi
Name:Npl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1921341. Npl.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320N-acetylneuraminate lyasePRO_0000273353Add
BLAST

Proteomic databases

MaxQBiQ9DCJ9.
PaxDbiQ9DCJ9.
PRIDEiQ9DCJ9.

PTM databases

PhosphoSiteiQ9DCJ9.

Expressioni

Gene expression databases

BgeeiQ9DCJ9.
CleanExiMM_NPL.
GenevestigatoriQ9DCJ9.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiQ9DCJ9. 1 interaction.
MINTiMINT-4128676.
STRINGi10090.ENSMUSP00000037454.

Structurei

3D structure databases

ProteinModelPortaliQ9DCJ9.
SMRiQ9DCJ9. Positions 8-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 522Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the DapA family. NanA subfamily.Curated

Phylogenomic databases

eggNOGiCOG0329.
GeneTreeiENSGT00530000063604.
HOGENOMiHOG000218206.
HOVERGENiHBG082055.
InParanoidiQ9DCJ9.
KOiK01639.
OMAiLQAFAKP.
OrthoDBiEOG7NKKKP.
PhylomeDBiQ9DCJ9.
TreeFamiTF353639.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.

Sequencei

Sequence statusi: Complete.

Q9DCJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFPKKKLRG LVAATITPMT ENGEINFPVI GQYVDYLVKE QGVKNIFVNG
60 70 80 90 100
TTGEGLSLSV SERRQVAEEW VNQGRNKLDQ VVIHVGALNV KESQELAQHA
110 120 130 140 150
AEIGADGIAV IAPFFFKSQN KDALISFLRE VAAAAPTLPF YYYHMPSMTG
160 170 180 190 200
VKIRAEELLD GIQDKIPTFQ GLKFTDTDLL DFGQCVDQNH QRQFALLFGV
210 220 230 240 250
DEQLLSALVM GATGAVGSTY NYLGKKTNQM LEAFEQKDLA SALSYQFRIQ
260 270 280 290 300
RFINYVIKLG FGVSQTKAIM TLVSGIPMGP PRLPLQKATQ EFTAKAEAKL
310 320
KSLDFLSSPS VKEGKPLASA
Length:320
Mass (Da):35,130
Last modified:June 1, 2001 - v1
Checksum:i9426AD7CC8438468
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002734 mRNA. Translation: BAB22314.1.
AK088859 mRNA. Translation: BAC40618.1.
BC022734 mRNA. Translation: AAH22734.1.
CCDSiCCDS15374.1.
RefSeqiNP_083025.1. NM_028749.1.
UniGeneiMm.24887.

Genome annotation databases

EnsembliENSMUST00000041874; ENSMUSP00000037454; ENSMUSG00000042684.
GeneIDi74091.
KEGGimmu:74091.
UCSCiuc007dac.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002734 mRNA. Translation: BAB22314.1.
AK088859 mRNA. Translation: BAC40618.1.
BC022734 mRNA. Translation: AAH22734.1.
CCDSiCCDS15374.1.
RefSeqiNP_083025.1. NM_028749.1.
UniGeneiMm.24887.

3D structure databases

ProteinModelPortaliQ9DCJ9.
SMRiQ9DCJ9. Positions 8-280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9DCJ9. 1 interaction.
MINTiMINT-4128676.
STRINGi10090.ENSMUSP00000037454.

PTM databases

PhosphoSiteiQ9DCJ9.

Proteomic databases

MaxQBiQ9DCJ9.
PaxDbiQ9DCJ9.
PRIDEiQ9DCJ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041874; ENSMUSP00000037454; ENSMUSG00000042684.
GeneIDi74091.
KEGGimmu:74091.
UCSCiuc007dac.1. mouse.

Organism-specific databases

CTDi80896.
MGIiMGI:1921341. Npl.

Phylogenomic databases

eggNOGiCOG0329.
GeneTreeiENSGT00530000063604.
HOGENOMiHOG000218206.
HOVERGENiHBG082055.
InParanoidiQ9DCJ9.
KOiK01639.
OMAiLQAFAKP.
OrthoDBiEOG7NKKKP.
PhylomeDBiQ9DCJ9.
TreeFamiTF353639.

Enzyme and pathway databases

UniPathwayiUPA00629.
ReactomeiREACT_202500. Sialic acid metabolism.

Miscellaneous databases

NextBioi339741.
PROiQ9DCJ9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9DCJ9.
CleanExiMM_NPL.
GenevestigatoriQ9DCJ9.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Kidney and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  3. "Metabolism of vertebrate amino sugars with N-glycolyl groups: elucidating the intracellular fate of the non-human sialic acid N-glycolylneuraminic acid."
    Bergfeld A.K., Pearce O.M., Diaz S.L., Pham T., Varki A.
    J. Biol. Chem. 287:28865-28881(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY.

Entry informationi

Entry nameiNPL_MOUSE
AccessioniPrimary (citable) accession number: Q9DCJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: June 1, 2001
Last modified: January 7, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.