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Protein

N-acetylneuraminate lyase

Gene

Npl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway.1 Publication

Catalytic activityi

N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.1 Publication

Pathwayi: N-acetylneuraminate degradation

This protein is involved in the pathway N-acetylneuraminate degradation, which is part of Amino-sugar metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway N-acetylneuraminate degradation and in Amino-sugar metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei143Involved in proton transfer during cleavageBy similarity1
Active sitei173Schiff-base intermediate with substrateBy similarity1

GO - Molecular functioni

  • N-acetylneuraminate lyase activity Source: UniProtKB

GO - Biological processi

  • carbohydrate metabolic process Source: UniProtKB-KW
  • N-acetylneuraminate catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

ReactomeiR-MMU-4085001. Sialic acid metabolism.
UniPathwayiUPA00629.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylneuraminate lyase (EC:4.1.3.3)
Short name:
NALase
Alternative name(s):
N-acetylneuraminate pyruvate-lyase
N-acetylneuraminic acid aldolase
Sialate lyase
Sialate-pyruvate lyase
Sialic acid aldolase
Sialic acid lyase
Gene namesi
Name:Npl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1921341. Npl.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002733531 – 320N-acetylneuraminate lyaseAdd BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei308PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9DCJ9.
MaxQBiQ9DCJ9.
PaxDbiQ9DCJ9.
PeptideAtlasiQ9DCJ9.
PRIDEiQ9DCJ9.

PTM databases

iPTMnetiQ9DCJ9.
PhosphoSitePlusiQ9DCJ9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000042684.
CleanExiMM_NPL.
GenevisibleiQ9DCJ9. MM.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiQ9DCJ9. 1 interactor.
MINTiMINT-4128676.
STRINGi10090.ENSMUSP00000037454.

Structurei

3D structure databases

ProteinModelPortaliQ9DCJ9.
SMRiQ9DCJ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 52Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the DapA family. NanA subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IGJ4. Eukaryota.
COG0329. LUCA.
GeneTreeiENSGT00530000063604.
HOGENOMiHOG000218206.
HOVERGENiHBG082055.
InParanoidiQ9DCJ9.
KOiK01639.
OMAiQEYSHEQ.
OrthoDBiEOG091G0AT4.
PhylomeDBiQ9DCJ9.
TreeFamiTF353639.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
SMARTiSM01130. DHDPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DCJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFPKKKLRG LVAATITPMT ENGEINFPVI GQYVDYLVKE QGVKNIFVNG
60 70 80 90 100
TTGEGLSLSV SERRQVAEEW VNQGRNKLDQ VVIHVGALNV KESQELAQHA
110 120 130 140 150
AEIGADGIAV IAPFFFKSQN KDALISFLRE VAAAAPTLPF YYYHMPSMTG
160 170 180 190 200
VKIRAEELLD GIQDKIPTFQ GLKFTDTDLL DFGQCVDQNH QRQFALLFGV
210 220 230 240 250
DEQLLSALVM GATGAVGSTY NYLGKKTNQM LEAFEQKDLA SALSYQFRIQ
260 270 280 290 300
RFINYVIKLG FGVSQTKAIM TLVSGIPMGP PRLPLQKATQ EFTAKAEAKL
310 320
KSLDFLSSPS VKEGKPLASA
Length:320
Mass (Da):35,130
Last modified:June 1, 2001 - v1
Checksum:i9426AD7CC8438468
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002734 mRNA. Translation: BAB22314.1.
AK088859 mRNA. Translation: BAC40618.1.
BC022734 mRNA. Translation: AAH22734.1.
CCDSiCCDS15374.1.
RefSeqiNP_083025.1. NM_028749.1.
UniGeneiMm.24887.

Genome annotation databases

EnsembliENSMUST00000041874; ENSMUSP00000037454; ENSMUSG00000042684.
GeneIDi74091.
KEGGimmu:74091.
UCSCiuc007dac.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002734 mRNA. Translation: BAB22314.1.
AK088859 mRNA. Translation: BAC40618.1.
BC022734 mRNA. Translation: AAH22734.1.
CCDSiCCDS15374.1.
RefSeqiNP_083025.1. NM_028749.1.
UniGeneiMm.24887.

3D structure databases

ProteinModelPortaliQ9DCJ9.
SMRiQ9DCJ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9DCJ9. 1 interactor.
MINTiMINT-4128676.
STRINGi10090.ENSMUSP00000037454.

PTM databases

iPTMnetiQ9DCJ9.
PhosphoSitePlusiQ9DCJ9.

Proteomic databases

EPDiQ9DCJ9.
MaxQBiQ9DCJ9.
PaxDbiQ9DCJ9.
PeptideAtlasiQ9DCJ9.
PRIDEiQ9DCJ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041874; ENSMUSP00000037454; ENSMUSG00000042684.
GeneIDi74091.
KEGGimmu:74091.
UCSCiuc007dac.1. mouse.

Organism-specific databases

CTDi80896.
MGIiMGI:1921341. Npl.

Phylogenomic databases

eggNOGiENOG410IGJ4. Eukaryota.
COG0329. LUCA.
GeneTreeiENSGT00530000063604.
HOGENOMiHOG000218206.
HOVERGENiHBG082055.
InParanoidiQ9DCJ9.
KOiK01639.
OMAiQEYSHEQ.
OrthoDBiEOG091G0AT4.
PhylomeDBiQ9DCJ9.
TreeFamiTF353639.

Enzyme and pathway databases

UniPathwayiUPA00629.
ReactomeiR-MMU-4085001. Sialic acid metabolism.

Miscellaneous databases

PROiQ9DCJ9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042684.
CleanExiMM_NPL.
GenevisibleiQ9DCJ9. MM.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
SMARTiSM01130. DHDPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNPL_MOUSE
AccessioniPrimary (citable) accession number: Q9DCJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.