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Protein

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

Gene

Ndufa8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.
R-MMU-6799198. Complex I biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
Alternative name(s):
Complex I-19kD
Short name:
CI-19kD
Complex I-PGIV
Short name:
CI-PGIV
NADH-ubiquinone oxidoreductase 19 kDa subunit
Gene namesi
Name:Ndufa8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1915625. Ndufa8.

Subcellular locationi

  • Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity
  • Mitochondrion intermembrane space By similarity

GO - Cellular componenti

  • mitochondrial inner membrane Source: MGI
  • mitochondrial intermembrane space Source: MGI
  • mitochondrial respiratory chain complex I Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001187352 – 172NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8Add BLAST171

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 66PROSITE-ProRule annotation
Disulfide bondi46 ↔ 56PROSITE-ProRule annotation
Disulfide bondi78 ↔ 110PROSITE-ProRule annotation
Disulfide bondi88 ↔ 100PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiQ9DCJ5.
MaxQBiQ9DCJ5.
PaxDbiQ9DCJ5.
PeptideAtlasiQ9DCJ5.
PRIDEiQ9DCJ5.

PTM databases

iPTMnetiQ9DCJ5.
PhosphoSitePlusiQ9DCJ5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026895.
GenevisibleiQ9DCJ5. MM.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212827. 4 interactors.
IntActiQ9DCJ5. 7 interactors.
MINTiMINT-4104492.
STRINGi10090.ENSMUSP00000065352.

Structurei

3D structure databases

ProteinModelPortaliQ9DCJ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 74CHCH 1PROSITE-ProRule annotationAdd BLAST42
Domaini75 – 118CHCH 2PROSITE-ProRule annotationAdd BLAST44

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi36 – 46Cx9C motif 1PROSITE-ProRule annotationAdd BLAST11
Motifi56 – 66Cx9C motif 2PROSITE-ProRule annotationAdd BLAST11
Motifi78 – 88Cx9C motif 3PROSITE-ProRule annotationAdd BLAST11
Motifi100 – 110Cx9C motif 4PROSITE-ProRule annotationAdd BLAST11

Domaini

Contains four C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds.By similarity

Sequence similaritiesi

Belongs to the complex I NDUFA8 subunit family.Curated
Contains 2 CHCH (coiled coil-helix-coiled coil-helix) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3458. Eukaryota.
ENOG4111IST. LUCA.
GeneTreeiENSGT00390000008938.
HOGENOMiHOG000090856.
HOVERGENiHBG001244.
InParanoidiQ9DCJ5.
KOiK03952.
OMAiYWTCLDY.
OrthoDBiEOG091G0M0S.
PhylomeDBiQ9DCJ5.
TreeFamiTF105633.

Family and domain databases

InterProiIPR010625. CHCH.
IPR016680. NADH_Ub_cplx-1_asu_su-8.
[Graphical view]
PfamiPF06747. CHCH. 1 hit.
[Graphical view]
PIRSFiPIRSF017016. NDUA8. 1 hit.
PROSITEiPS51808. CHCH. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DCJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGIVELPTL EELKVEEVKV SSAVLKAAAH HYGAQCDKTN KEFMLCRWEE
60 70 80 90 100
KDPRRCLKEG KLVNGCALNF FRQIKSHCAE PFTEYWTCLD YSNMQLFRHC
110 120 130 140 150
RQQQAKFDQC VLDKLGWVRP DLGQLSKVTK VKTDRPLPEN PYHSRARPEP
160 170
NPVIEGDLKP AKHGTRFFFW TV
Length:172
Mass (Da):19,992
Last modified:January 23, 2007 - v3
Checksum:i7DF62AB86B5BF684
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002744 mRNA. Translation: BAB22322.1.
AL773525 Genomic DNA. Translation: CAM16134.1.
BC012416 mRNA. Translation: AAH12416.1.
BC024673 mRNA. Translation: AAH24673.1.
CCDSiCCDS15967.1.
RefSeqiNP_080979.1. NM_026703.2.
UniGeneiMm.19834.

Genome annotation databases

EnsembliENSMUST00000070112; ENSMUSP00000065352; ENSMUSG00000026895.
GeneIDi68375.
KEGGimmu:68375.
UCSCiuc008jlb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002744 mRNA. Translation: BAB22322.1.
AL773525 Genomic DNA. Translation: CAM16134.1.
BC012416 mRNA. Translation: AAH12416.1.
BC024673 mRNA. Translation: AAH24673.1.
CCDSiCCDS15967.1.
RefSeqiNP_080979.1. NM_026703.2.
UniGeneiMm.19834.

3D structure databases

ProteinModelPortaliQ9DCJ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212827. 4 interactors.
IntActiQ9DCJ5. 7 interactors.
MINTiMINT-4104492.
STRINGi10090.ENSMUSP00000065352.

PTM databases

iPTMnetiQ9DCJ5.
PhosphoSitePlusiQ9DCJ5.

Proteomic databases

EPDiQ9DCJ5.
MaxQBiQ9DCJ5.
PaxDbiQ9DCJ5.
PeptideAtlasiQ9DCJ5.
PRIDEiQ9DCJ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070112; ENSMUSP00000065352; ENSMUSG00000026895.
GeneIDi68375.
KEGGimmu:68375.
UCSCiuc008jlb.1. mouse.

Organism-specific databases

CTDi4702.
MGIiMGI:1915625. Ndufa8.

Phylogenomic databases

eggNOGiKOG3458. Eukaryota.
ENOG4111IST. LUCA.
GeneTreeiENSGT00390000008938.
HOGENOMiHOG000090856.
HOVERGENiHBG001244.
InParanoidiQ9DCJ5.
KOiK03952.
OMAiYWTCLDY.
OrthoDBiEOG091G0M0S.
PhylomeDBiQ9DCJ5.
TreeFamiTF105633.

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.
R-MMU-6799198. Complex I biogenesis.

Miscellaneous databases

PROiQ9DCJ5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026895.
GenevisibleiQ9DCJ5. MM.

Family and domain databases

InterProiIPR010625. CHCH.
IPR016680. NADH_Ub_cplx-1_asu_su-8.
[Graphical view]
PfamiPF06747. CHCH. 1 hit.
[Graphical view]
PIRSFiPIRSF017016. NDUA8. 1 hit.
PROSITEiPS51808. CHCH. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDUA8_MOUSE
AccessioniPrimary (citable) accession number: Q9DCJ5
Secondary accession number(s): A2AL45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.