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Protein

AN1-type zinc finger protein 6

Gene

Zfand6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in regulation of TNF-alpha induced NF-kappa-B activation and apoptosis. Involved in modulation of 'Lys-48'-linked polyubiquitination status of TRAF2 and decreases association of TRAF2 with RIPK1 (By similarity). Required for PTS1 target sequence-dependent protein import into peroxisomes and PEX5 stability; may cooperate with PEX6. In vitro involved in PEX5 export from the cytosol to peroxisomes.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri8 – 4235A20-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri161 – 20444AN1-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: InterPro
  • polyubiquitin binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
AN1-type zinc finger protein 6
Alternative name(s):
Associated with PRK1 protein
Zinc finger A20 domain-containing protein 3
Gene namesi
Name:Zfand6
Synonyms:Awp1, Za20d3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1929510. Zfand6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi30 – 301C → S: Abolishes interaction with PEX6 and ubiquitin. Impairs interaction with PEX5. 1 Publication
Mutagenesisi180 – 1801C → S: Impairs interaction with PEX6. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223AN1-type zinc finger protein 6PRO_0000245236Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491PhosphoserineBy similarity
Modified residuei219 – 2191N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9DCH6.
MaxQBiQ9DCH6.
PaxDbiQ9DCH6.
PeptideAtlasiQ9DCH6.
PRIDEiQ9DCH6.

PTM databases

iPTMnetiQ9DCH6.
PhosphoSiteiQ9DCH6.

Expressioni

Gene expression databases

BgeeiQ9DCH6.
CleanExiMM_ZFAND6.
GenevisibleiQ9DCH6. MM.

Interactioni

Subunit structurei

Interacts with PKN1. Interacts with TRAF2 (By similarity). Interacts with mono- and polyubiquitin. Interacts with PEX6. Interacts with PEX5 (Cys-linked ubiquitinated).By similarity2 Publications

GO - Molecular functioni

  • polyubiquitin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi211125. 1 interaction.
STRINGi10090.ENSMUSP00000069228.

Structurei

3D structure databases

ProteinModelPortaliQ9DCH6.
SMRiQ9DCH6. Positions 10-44, 150-205.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The A20-type zinc finger domain mediates regulation of NF-kappa-B activity.By similarity
The AN1-type zinc finger domain mediates association with TRAF2.By similarity

Sequence similaritiesi

Contains 1 A20-type zinc finger.PROSITE-ProRule annotation
Contains 1 AN1-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri8 – 4235A20-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri161 – 20444AN1-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3173. Eukaryota.
ENOG4111UWC. LUCA.
GeneTreeiENSGT00510000046525.
HOGENOMiHOG000238229.
HOVERGENiHBG053349.
InParanoidiQ9DCH6.
OMAiNVPEAQS.
OrthoDBiEOG7TTQ9W.
PhylomeDBiQ9DCH6.
TreeFamiTF313612.

Family and domain databases

Gene3Di4.10.1110.10. 1 hit.
InterProiIPR002653. Znf_A20.
IPR000058. Znf_AN1.
[Graphical view]
PfamiPF01754. zf-A20. 1 hit.
PF01428. zf-AN1. 1 hit.
[Graphical view]
SMARTiSM00259. ZnF_A20. 1 hit.
SM00154. ZnF_AN1. 1 hit.
[Graphical view]
PROSITEiPS51036. ZF_A20. 1 hit.
PS51039. ZF_AN1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DCH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQETNHSQA PMLCSTGCGF YGNPRTNGMC SVCYKEHLQR QNSSNGRISP
60 70 80 90 100
PAASVSSLSE SLPVQCADGS VPDAQSALDS TSSSMQPGPV SNQSLLSESV
110 120 130 140 150
APSQVDSTSV DKAVSETEDL QGPRAEGLVP LECDPPSSVS DTTQQPSEEQ
160 170 180 190 200
SKSLEKPKQK KNRCFMCRKK VGLTGFECRC GNVYCGVHRY SDVHNCSYNY
210 220
KADAAEKIRK ENPVVVGEKI QKI
Length:223
Mass (Da):24,001
Last modified:June 1, 2001 - v1
Checksum:iFFBF1A09EEC39901
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti99 – 991S → P in CAC14886 (PubMed:11054541).Curated
Sequence conflicti190 – 1901Y → S in CAC14886 (PubMed:11054541).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251508 mRNA. Translation: CAC14886.1.
AK002775 mRNA. Translation: BAB22349.1.
AK144902 mRNA. Translation: BAE26124.1.
BC010683 mRNA. Translation: AAH10683.1.
CCDSiCCDS21419.1.
RefSeqiNP_075361.2. NM_022985.6.
XP_006508168.1. XM_006508105.1.
XP_006508169.1. XM_006508106.2.
XP_006508170.1. XM_006508107.2.
XP_006508171.1. XM_006508108.1.
UniGeneiMm.209349.
Mm.397115.

Genome annotation databases

EnsembliENSMUST00000069537; ENSMUSP00000069228; ENSMUSG00000030629.
ENSMUST00000178385; ENSMUSP00000135968; ENSMUSG00000030629.
ENSMUST00000209117; ENSMUSP00000146518; ENSMUSG00000030629.
GeneIDi65098.
KEGGimmu:65098.
UCSCiuc009iek.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251508 mRNA. Translation: CAC14886.1.
AK002775 mRNA. Translation: BAB22349.1.
AK144902 mRNA. Translation: BAE26124.1.
BC010683 mRNA. Translation: AAH10683.1.
CCDSiCCDS21419.1.
RefSeqiNP_075361.2. NM_022985.6.
XP_006508168.1. XM_006508105.1.
XP_006508169.1. XM_006508106.2.
XP_006508170.1. XM_006508107.2.
XP_006508171.1. XM_006508108.1.
UniGeneiMm.209349.
Mm.397115.

3D structure databases

ProteinModelPortaliQ9DCH6.
SMRiQ9DCH6. Positions 10-44, 150-205.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211125. 1 interaction.
STRINGi10090.ENSMUSP00000069228.

PTM databases

iPTMnetiQ9DCH6.
PhosphoSiteiQ9DCH6.

Proteomic databases

EPDiQ9DCH6.
MaxQBiQ9DCH6.
PaxDbiQ9DCH6.
PeptideAtlasiQ9DCH6.
PRIDEiQ9DCH6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000069537; ENSMUSP00000069228; ENSMUSG00000030629.
ENSMUST00000178385; ENSMUSP00000135968; ENSMUSG00000030629.
ENSMUST00000209117; ENSMUSP00000146518; ENSMUSG00000030629.
GeneIDi65098.
KEGGimmu:65098.
UCSCiuc009iek.1. mouse.

Organism-specific databases

CTDi54469.
MGIiMGI:1929510. Zfand6.

Phylogenomic databases

eggNOGiKOG3173. Eukaryota.
ENOG4111UWC. LUCA.
GeneTreeiENSGT00510000046525.
HOGENOMiHOG000238229.
HOVERGENiHBG053349.
InParanoidiQ9DCH6.
OMAiNVPEAQS.
OrthoDBiEOG7TTQ9W.
PhylomeDBiQ9DCH6.
TreeFamiTF313612.

Miscellaneous databases

ChiTaRSiZfand6. mouse.
PROiQ9DCH6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9DCH6.
CleanExiMM_ZFAND6.
GenevisibleiQ9DCH6. MM.

Family and domain databases

Gene3Di4.10.1110.10. 1 hit.
InterProiIPR002653. Znf_A20.
IPR000058. Znf_AN1.
[Graphical view]
PfamiPF01754. zf-A20. 1 hit.
PF01428. zf-AN1. 1 hit.
[Graphical view]
SMARTiSM00259. ZnF_A20. 1 hit.
SM00154. ZnF_AN1. 1 hit.
[Graphical view]
PROSITEiPS51036. ZF_A20. 1 hit.
PS51039. ZF_AN1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of AWP1, a novel protein that associates with serine/threonine kinase PRK1 in vivo."
    Duan W., Sun B., Li T.W., Tan B.J., Lee M.K., Teo T.S.
    Gene 256:113-121(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH PKN1.
    Tissue: Liver, Mammary gland and Uterus.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Kidney and Lung.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "AWP1/ZFAND6 functions in Pex5 export by interacting with cys-monoubiquitinated Pex5 and Pex6 AAA ATPase."
    Miyata N., Okumoto K., Mukai S., Noguchi M., Fujiki Y.
    Traffic 13:168-183(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, UBIQUITIN-BINDING, INTERACTION WITH PEX6 AND PEX5, MUTAGENESIS OF CYS-30 AND CYS-180.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-219, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiZFAN6_MOUSE
AccessioniPrimary (citable) accession number: Q9DCH6
Secondary accession number(s): Q9ER79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.