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Q9DCH4

- EIF3F_MOUSE

UniProt

Q9DCH4 - EIF3F_MOUSE

Protein

Eukaryotic translation initiation factor 3 subunit F

Gene

Eif3f

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
    Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. translation initiation factor activity Source: UniProtKB-HAMAP
    3. translation initiation factor binding Source: MGI
    4. ubiquitin-specific protease activity Source: FlyBase

    GO - Biological processi

    1. formation of translation preinitiation complex Source: UniProtKB-HAMAP
    2. protein deubiquitination Source: FlyBase
    3. regulation of translational initiation Source: UniProtKB-HAMAP
    4. translational initiation Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Initiation factor, Protease, Thiol protease

    Keywords - Biological processi

    Protein biosynthesis, Ubl conjugation pathway

    Protein family/group databases

    MEROPSiM67.974.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic translation initiation factor 3 subunit FUniRule annotation
    Short name:
    eIF3fUniRule annotation
    Alternative name(s):
    Deubiquitinating enzyme eIF3f (EC:3.4.19.12)
    Eukaryotic translation initiation factor 3 subunit 5UniRule annotation
    eIF-3-epsilonUniRule annotation
    eIF3 p47UniRule annotation
    Gene namesi
    Name:Eif3f
    Synonyms:Eif3s5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1913335. Eif3f.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. eukaryotic 43S preinitiation complex Source: UniProtKB-HAMAP
    2. eukaryotic 48S preinitiation complex Source: UniProtKB-HAMAP
    3. eukaryotic translation initiation factor 3 complex Source: UniProtKB
    4. eukaryotic translation initiation factor 3 complex, eIF3m Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedUniRule annotation
    Chaini2 – 361360Eukaryotic translation initiation factor 3 subunit FPRO_0000213965Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineUniRule annotation
    Modified residuei52 – 521Phosphoserine; by CDK11; in vitroUniRule annotation
    Modified residuei242 – 2421N6-acetyllysineBy similarity
    Modified residuei262 – 2621PhosphoserineUniRule annotation

    Post-translational modificationi

    Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11 By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9DCH4.
    PaxDbiQ9DCH4.
    PRIDEiQ9DCH4.

    PTM databases

    PhosphoSiteiQ9DCH4.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9DCH4.
    BgeeiQ9DCH4.
    GenevestigatoriQ9DCH4.

    Interactioni

    Subunit structurei

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex may interact with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation may lead to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RNF139; the interaction leads to protein translation inhibitions in a ubiquitination-dependent manner. Interacts with DTX1, the interaction is required for deubiquitinating activity towards NOTCH1 By similarity.UniRule annotation

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MtorQ9JLN95EBI-1634316,EBI-1571628

    Protein-protein interaction databases

    BioGridi211203. 2 interactions.
    DIPiDIP-42766N.
    IntActiQ9DCH4. 8 interactions.
    MINTiMINT-1899046.
    STRINGi10090.ENSMUSP00000033342.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9DCH4.
    SMRiQ9DCH4. Positions 94-360.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini91 – 200110MPNUniRule annotationAdd
    BLAST

    Domaini

    The MPN domain mediates deubiquitinating activity.By similarity

    Sequence similaritiesi

    Belongs to the eIF-3 subunit F family.UniRule annotation
    Contains 1 MPN (JAB/Mov34) domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1310.
    GeneTreeiENSGT00530000063075.
    HOGENOMiHOG000241154.
    HOVERGENiHBG107843.
    InParanoidiQ5XJV3.
    KOiK03249.
    OMAiHPVVLFQ.
    OrthoDBiEOG77T152.
    TreeFamiTF101517.

    Family and domain databases

    HAMAPiMF_03005. eIF3f.
    InterProiIPR027531. eIF3f.
    IPR000555. JAMM/MPN+_dom.
    IPR024969. Rpn11/EIF3F_C.
    [Graphical view]
    PfamiPF01398. JAB. 1 hit.
    PF13012. MitMem_reg. 1 hit.
    [Graphical view]
    SMARTiSM00232. JAB_MPN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9DCH4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASPAVPANV PPATAAAAPA PVVTAAPASA PTPSTPAPTP AATPAASPAP    50
    VSSDPAVAAP AAPGQTPASA PAPAQTPAPS QPGPALPGPF PGGRVVRLHP 100
    VILASIVDSY ERRNEGAARV IGTLLGTVDK HSVEVTNCFS VPHNESEDEV 150
    AVDMEFAKNM YELHKKVSPN ELILGWYATG HDITEHSVLI HEYYSREAPN 200
    PIHLTVDTGL QHGRMSIKAY VSTLMGVPGR TMGVMFTPLT VKYAYYDTER 250
    IGVDLIMKTC FSPNRVIGLS SDLQQVGGAS ARIQDALSTV LQYAEDVLSG 300
    KVSADNTVGR FLMSLVNQVP KIVPDDFETM LNSNINDLLM VTYLANLTQS 350
    QIALNEKLVN L 361
    Length:361
    Mass (Da):37,984
    Last modified:July 27, 2011 - v2
    Checksum:iD0BE3309F9141E40
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti198 – 1981A → S in BAB22352. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK002778 mRNA. Translation: BAB22352.1.
    CH466531 Genomic DNA. Translation: EDL16920.1.
    BC083190 mRNA. Translation: AAH83190.1.
    CCDSiCCDS21731.1.
    RefSeqiNP_079620.2. NM_025344.2.
    UniGeneiMm.182962.

    Genome annotation databases

    EnsembliENSMUST00000033342; ENSMUSP00000033342; ENSMUSG00000031029.
    GeneIDi66085.
    KEGGimmu:66085.
    UCSCiuc009jdc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK002778 mRNA. Translation: BAB22352.1 .
    CH466531 Genomic DNA. Translation: EDL16920.1 .
    BC083190 mRNA. Translation: AAH83190.1 .
    CCDSi CCDS21731.1.
    RefSeqi NP_079620.2. NM_025344.2.
    UniGenei Mm.182962.

    3D structure databases

    ProteinModelPortali Q9DCH4.
    SMRi Q9DCH4. Positions 94-360.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 211203. 2 interactions.
    DIPi DIP-42766N.
    IntActi Q9DCH4. 8 interactions.
    MINTi MINT-1899046.
    STRINGi 10090.ENSMUSP00000033342.

    Protein family/group databases

    MEROPSi M67.974.

    PTM databases

    PhosphoSitei Q9DCH4.

    Proteomic databases

    MaxQBi Q9DCH4.
    PaxDbi Q9DCH4.
    PRIDEi Q9DCH4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033342 ; ENSMUSP00000033342 ; ENSMUSG00000031029 .
    GeneIDi 66085.
    KEGGi mmu:66085.
    UCSCi uc009jdc.2. mouse.

    Organism-specific databases

    CTDi 8665.
    MGIi MGI:1913335. Eif3f.

    Phylogenomic databases

    eggNOGi COG1310.
    GeneTreei ENSGT00530000063075.
    HOGENOMi HOG000241154.
    HOVERGENi HBG107843.
    InParanoidi Q5XJV3.
    KOi K03249.
    OMAi HPVVLFQ.
    OrthoDBi EOG77T152.
    TreeFami TF101517.

    Miscellaneous databases

    ChiTaRSi EIF3F. mouse.
    NextBioi 320584.
    PROi Q9DCH4.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9DCH4.
    Bgeei Q9DCH4.
    Genevestigatori Q9DCH4.

    Family and domain databases

    HAMAPi MF_03005. eIF3f.
    InterProi IPR027531. eIF3f.
    IPR000555. JAMM/MPN+_dom.
    IPR024969. Rpn11/EIF3F_C.
    [Graphical view ]
    Pfami PF01398. JAB. 1 hit.
    PF13012. MitMem_reg. 1 hit.
    [Graphical view ]
    SMARTi SM00232. JAB_MPN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Kidney.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "mTOR-dependent stimulation of the association of eIF4G and eIF3 by insulin."
      Harris T.E., Chi A., Shabanowitz J., Hunt D.F., Rhoads R.E., Lawrence J.C. Jr.
      EMBO J. 25:1659-1668(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EIF3B AND MTOR.
    5. "Reconstitution reveals the functional core of mammalian eIF3."
      Masutani M., Sonenberg N., Yokoyama S., Imataka H.
      EMBO J. 26:3373-3383(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF THE EIF-3 COMPLEX, IDENTIFICATION IN THE EIF-3 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiEIF3F_MOUSE
    AccessioniPrimary (citable) accession number: Q9DCH4
    Secondary accession number(s): Q5XJV3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2002
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 93 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Translation initiation factors
      List of translation initiation factor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3