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Q9DCD6

- GBRAP_MOUSE

UniProt

Q9DCD6 - GBRAP_MOUSE

Protein

Gamma-aminobutyric acid receptor-associated protein

Gene

Gabarap

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Ubiquitin-like modifier that play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei116 – 1172Cleavage; by ATG4B

    GO - Molecular functioni

    1. microtubule binding Source: MGI
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. autophagy Source: UniProtKB-KW
    2. extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
    3. microtubule cytoskeleton organization Source: MGI
    4. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Apoptosis, Autophagy, Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gamma-aminobutyric acid receptor-associated protein
    Alternative name(s):
    GABA(A) receptor-associated protein
    Gene namesi
    Name:Gabarap
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1861742. Gabarap.

    Subcellular locationi

    Endomembrane system By similarity. Cytoplasmcytoskeleton By similarity. Golgi apparatus membrane By similarity. Cytoplasmic vesicleautophagosome By similarity
    Note: Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules By similarity. Localizes also to discrete punctae along the ciliary axoneme.By similarity3 Publications

    GO - Cellular componenti

    1. actin cytoskeleton Source: MGI
    2. autophagic vacuole Source: UniProtKB
    3. autophagic vacuole membrane Source: Ensembl
    4. axoneme Source: UniProtKB
    5. cell body Source: Ensembl
    6. cytoplasmic vesicle Source: UniProtKB-KW
    7. Golgi apparatus Source: MGI
    8. Golgi membrane Source: UniProtKB-SubCell
    9. lysosome Source: MGI
    10. microtubule Source: UniProtKB-KW
    11. microtubule associated complex Source: MGI
    12. perinuclear region of cytoplasm Source: Ensembl
    13. plasma membrane Source: MGI
    14. smooth endoplasmic reticulum Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Golgi apparatus, Membrane, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 116116Gamma-aminobutyric acid receptor-associated proteinPRO_0000212364Add
    BLAST
    Propeptidei117 – 1171Removed in mature formPRO_0000423066

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi116 – 1161Phosphatidylethanolamine amidated glycineCurated

    Post-translational modificationi

    The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.1 Publication

    Keywords - PTMi

    Lipoprotein

    Proteomic databases

    MaxQBiQ9DCD6.
    PaxDbiQ9DCD6.
    PRIDEiQ9DCD6.

    PTM databases

    PhosphoSiteiQ9DCD6.

    Expressioni

    Gene expression databases

    BgeeiQ9DCD6.
    GenevestigatoriQ9DCD6.

    Interactioni

    Subunit structurei

    Interacts with ATG3, ATG7, CALR, DDX47, GABRG2, TUBA1, ULK1 and NSF By similarity. Interacts with beta-tubulin and GPHN. Interacts with TP53INP1 and TP53INP2 By similarity. Interacts with TBC1D25. Directly interacts with SQSTM1 By similarity. Interacts with MAPK15 By similarity. Interacts with TECPR2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi208012. 2 interactions.
    STRINGi10090.ENSMUSP00000018711.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9DCD6.
    SMRiQ9DCD6. Positions 1-116.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2222Interaction with beta-tubulinAdd
    BLAST
    Regioni36 – 11782Interaction with GPHNAdd
    BLAST
    Regioni36 – 6833Interaction with GABRG2Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATG8 family.Curated

    Phylogenomic databases

    eggNOGiNOG263746.
    GeneTreeiENSGT00390000012937.
    HOGENOMiHOG000232034.
    HOVERGENiHBG051706.
    InParanoidiB1AR49.
    KOiK08341.
    OMAiTTMGQLY.
    OrthoDBiEOG70KGRK.
    PhylomeDBiQ9DCD6.
    TreeFamiTF314556.

    Family and domain databases

    InterProiIPR004241. Atg8_like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PANTHERiPTHR10969. PTHR10969. 1 hit.
    PfamiPF02991. Atg8. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9DCD6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL    50
    VPSDLTVGQF YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE 100
    EDFFLYIAYS DESVYGL 117
    Length:117
    Mass (Da):13,918
    Last modified:March 1, 2004 - v2
    Checksum:iBC0B84B8A51C1E32
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121E → D in BAB22426. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF161587 mRNA. Translation: AAD47642.1.
    AK002879 mRNA. Translation: BAB22426.1.
    AK011731 mRNA. Translation: BAB27806.1.
    AL596185 Genomic DNA. Translation: CAI35162.1.
    BC002126 mRNA. Translation: AAH02126.1.
    BC024621 mRNA. Translation: AAH24621.1.
    BC030350 mRNA. Translation: AAH30350.1.
    CCDSiCCDS24928.1.
    RefSeqiNP_062723.1. NM_019749.4.
    UniGeneiMm.272460.

    Genome annotation databases

    EnsembliENSMUST00000018711; ENSMUSP00000018711; ENSMUSG00000018567.
    GeneIDi56486.
    KEGGimmu:56486.
    UCSCiuc007jtg.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF161587 mRNA. Translation: AAD47642.1 .
    AK002879 mRNA. Translation: BAB22426.1 .
    AK011731 mRNA. Translation: BAB27806.1 .
    AL596185 Genomic DNA. Translation: CAI35162.1 .
    BC002126 mRNA. Translation: AAH02126.1 .
    BC024621 mRNA. Translation: AAH24621.1 .
    BC030350 mRNA. Translation: AAH30350.1 .
    CCDSi CCDS24928.1.
    RefSeqi NP_062723.1. NM_019749.4.
    UniGenei Mm.272460.

    3D structure databases

    ProteinModelPortali Q9DCD6.
    SMRi Q9DCD6. Positions 1-116.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 208012. 2 interactions.
    STRINGi 10090.ENSMUSP00000018711.

    PTM databases

    PhosphoSitei Q9DCD6.

    Proteomic databases

    MaxQBi Q9DCD6.
    PaxDbi Q9DCD6.
    PRIDEi Q9DCD6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000018711 ; ENSMUSP00000018711 ; ENSMUSG00000018567 .
    GeneIDi 56486.
    KEGGi mmu:56486.
    UCSCi uc007jtg.2. mouse.

    Organism-specific databases

    CTDi 11337.
    MGIi MGI:1861742. Gabarap.

    Phylogenomic databases

    eggNOGi NOG263746.
    GeneTreei ENSGT00390000012937.
    HOGENOMi HOG000232034.
    HOVERGENi HBG051706.
    InParanoidi B1AR49.
    KOi K08341.
    OMAi TTMGQLY.
    OrthoDBi EOG70KGRK.
    PhylomeDBi Q9DCD6.
    TreeFami TF314556.

    Miscellaneous databases

    ChiTaRSi GABARAP. mouse.
    NextBioi 312758.
    PROi Q9DCD6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9DCD6.
    Genevestigatori Q9DCD6.

    Family and domain databases

    InterProi IPR004241. Atg8_like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    PANTHERi PTHR10969. PTHR10969. 1 hit.
    Pfami PF02991. Atg8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Binding of the GABA(A) receptor-associated protein (GABARAP) to microtubules and microfilaments suggests involvement of the cytoskeleton in GABARAPGABA(A) receptor interaction."
      Wang H., Olsen R.W.
      J. Neurochem. 75:644-655(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH BETA-TUBULIN.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo and Kidney.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver and Mammary tumor.
    5. "Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs."
      Tanida I., Tanida-Miyake E., Nishitani T., Komatsu M., Yamazaki H., Ueno T., Kominami E.
      Biochem. Biophys. Res. Commun. 292:256-262(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG7.
    6. "A single protease, Apg4B, is specific for the autophagy-related ubiquitin-like proteins GATE-16, MAP1-LC3, GABARAP, and Apg8L."
      Hemelaar J., Lelyveld V.S., Kessler B.M., Ploegh H.L.
      J. Biol. Chem. 278:51841-51850(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE BY ATG4B, SUBCELLULAR LOCATION.
    7. "The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse."
      Kneussel M., Haverkamp S., Fuhrmann J.C., Wang H., Waessle H., Olsen R.W., Betz H.
      Proc. Natl. Acad. Sci. U.S.A. 97:8594-8599(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GPHN.
    8. "OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation."
      Itoh T., Kanno E., Uemura T., Waguri S., Fukuda M.
      J. Cell Biol. 192:839-853(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TBC1D25.
    9. Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiGBRAP_MOUSE
    AccessioniPrimary (citable) accession number: Q9DCD6
    Secondary accession number(s): B1AR49, Q9QUI7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: March 1, 2004
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3