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Protein

High mobility group nucleosome-binding domain-containing protein 3

Gene

Hmgn3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to nucleosomes, regulating chromatin structure and consequently, chromatin-dependent processes such as transcription, DNA replication and DNA repair. Affects both insulin and glucagon levels and modulates the expression of pancreatic genes involved in insulin secretion. Regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors. Regulates the expression of SLC6A9, a glycine transporter which regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. May play a role in ocular development and astrocyte function.5 Publications

GO - Molecular functioni

  • chromatin binding Source: MGI
  • nucleosomal DNA binding Source: InterPro

GO - Biological processi

  • chromatin organization Source: UniProtKB-KW
  • positive regulation of DNA binding transcription factor activity Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • regulation of insulin secretion involved in cellular response to glucose stimulus Source: MGI
  • regulation of transcription by RNA polymerase II Source: MGI

Keywordsi

Molecular functionChromatin regulator, DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
High mobility group nucleosome-binding domain-containing protein 3
Gene namesi
Name:Hmgn3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2138069 Hmgn3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile. Mice have a mild diabetic phenotype and lower plasma glucagon levels. The overall shape of the islets, the location of the alpha cells in the mantle of the pancreatic islets or proliferation of pancreatic alpha cells are not affected.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002325751 – 99High mobility group nucleosome-binding domain-containing protein 3Add BLAST99

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphoserineBy similarity1
Modified residuei10PhosphothreonineBy similarity1
Modified residuei78PhosphoserineBy similarity1
Modified residuei93PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9DCB1
PeptideAtlasiQ9DCB1
PRIDEiQ9DCB1
TopDownProteomicsiQ9DCB1-1 [Q9DCB1-1]
Q9DCB1-2 [Q9DCB1-2]

PTM databases

iPTMnetiQ9DCB1
PhosphoSitePlusiQ9DCB1

Expressioni

Tissue specificityi

Expressed in the brain, eye, prostate, thyroid, kidney, testis, glial cells and insulin-producing cells of the Langerhans pancreatic islets. In the brain, expressed in the lateral olfactory tract, anterior commissure, corpus callosum, internal capsule, fornix, stria medullans, optic tract, axon bundles, Purkinje cell layer and granular layer of the cerebellum. In retina, expressed in the nuclei of cells in the inner nuclear layer including amacrine, bipolar and horizontal neurons and in the nuclei of ganglion neurons. Detected at low levels in the liver.6 Publications

Developmental stagei

Transiently expressed in the stroma and endothelium of the cornea at birth. Subsequently expressed in the corneal epithelium and the inner nuclear and ganglion cell layers of the retina. The predominant form in developing ocular tissues is isoform 2, although isoform 1 is also detectable.1 Publication

Gene expression databases

BgeeiENSMUSG00000066456
CleanExiMM_HMGN3
ExpressionAtlasiQ9DCB1 baseline and differential
GenevisibleiQ9DCB1 MM

Interactioni

Subunit structurei

Interacts with the ligand binding domain of the thyroid receptor (TR) (in vitro). Requires the presence of thyroid hormone for its interaction. Interacts with nucleosomes (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9DCB1, 1 interactor
MINTiQ9DCB1
STRINGi10090.ENSMUSP00000124278

Family & Domainsi

Sequence similaritiesi

Belongs to the HMGN family.Curated

Phylogenomic databases

eggNOGiENOG410J0Z7 Eukaryota
ENOG410YTHP LUCA
GeneTreeiENSGT00730000111287
HOGENOMiHOG000116395
HOVERGENiHBG073479
InParanoidiQ9DCB1
KOiK11301
PhylomeDBiQ9DCB1

Family and domain databases

InterProiView protein in InterPro
IPR031073 HMGN3
IPR000079 HMGN_fam
PANTHERiPTHR23087:SF2 PTHR23087:SF2, 1 hit
PfamiView protein in Pfam
PF01101 HMG14_17, 1 hit
PRINTSiPR00925 NONHISHMG17
SMARTiView protein in SMART
SM00527 HMG17, 1 hit
PROSITEiView protein in PROSITE
PS00355 HMG14_17, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9DCB1-1) [UniParc]FASTAAdd to basket
Also known as: HMGN3a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPKRKSPENT EGKDGTKLTK QEPTRRSARL SAKPVPPKPE SKPRKTSAKK
60 70 80 90
EPGTKISRGA KGKKEEKQEA GEEGTAPSAN GDTKVEEAQR TESIEKEGE
Length:99
Mass (Da):10,769
Last modified:June 1, 2001 - v1
Checksum:iDE0277EFBE8232CF
GO
Isoform 2 (identifier: Q9DCB1-2) [UniParc]FASTAAdd to basket
Also known as: HMGN3b

The sequence of this isoform differs from the canonical sequence as follows:
     76-77: AP → EN
     78-99: Missing.

Show »
Length:77
Mass (Da):8,455
Checksum:iB7340DA18071905B
GO
Isoform 3 (identifier: Q9DCB1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     88-95: AQRTESIE → VLSTNTSH
     96-99: Missing.

Note: No experimental confirmation available.
Show »
Length:95
Mass (Da):10,250
Checksum:i5F67D6D091D77319
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01790976 – 77AP → EN in isoform 2. Curated2
Alternative sequenceiVSP_01791078 – 99Missing in isoform 2. CuratedAdd BLAST22
Alternative sequenceiVSP_01791188 – 95AQRTESIE → VLSTNTSH in isoform 3. 1 Publication8
Alternative sequenceiVSP_01791296 – 99Missing in isoform 3. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002970 mRNA Translation: BAB22485.1
AK153223 mRNA Translation: BAE31816.1
BC005693 mRNA Translation: AAH05693.1
BK000004 mRNA Translation: DAA00393.1
BK000005 mRNA Translation: DAA00394.1
CCDSiCCDS52872.1 [Q9DCB1-1]
CCDS52873.1 [Q9DCB1-2]
RefSeqiNP_080398.1, NM_026122.4 [Q9DCB1-1]
NP_778249.1, NM_175074.2 [Q9DCB1-2]
UniGeneiMm.244426

Genome annotation databases

EnsembliENSMUST00000161796; ENSMUSP00000125616; ENSMUSG00000066456 [Q9DCB1-2]
ENSMUST00000162246; ENSMUSP00000124278; ENSMUSG00000066456 [Q9DCB1-1]
ENSMUST00000185315; ENSMUSP00000140356; ENSMUSG00000066456 [Q9DCB1-3]
ENSMUST00000190154; ENSMUSP00000140247; ENSMUSG00000066456 [Q9DCB1-3]
GeneIDi94353
KEGGimmu:94353
UCSCiuc009qwc.3 mouse [Q9DCB1-3]
uc009qwd.2 mouse [Q9DCB1-2]
uc009qwe.2 mouse [Q9DCB1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHMGN3_MOUSE
AccessioniPrimary (citable) accession number: Q9DCB1
Secondary accession number(s): Q7M737, Q99JU1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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