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Q9DCB1

- HMGN3_MOUSE

UniProt

Q9DCB1 - HMGN3_MOUSE

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Protein

High mobility group nucleosome-binding domain-containing protein 3

Gene

Hmgn3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Binds to nucleosomes, regulating chromatin structure and consequently, chromatin-dependent processes such as transcription, DNA replication and DNA repair. Affects both insulin and glucagon levels and modulates the expression of pancreatic genes involved in insulin secretion. Regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors. Regulates the expression of SLC6A9, a glycine transporter which regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. May play a role in ocular development and astrocyte function.5 Publications

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. chromatin modification Source: UniProtKB-KW
  2. positive regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  3. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  4. regulation of insulin secretion involved in cellular response to glucose stimulus Source: MGI
  5. regulation of transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
High mobility group nucleosome-binding domain-containing protein 3
Gene namesi
Name:Hmgn3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:2138069. Hmgn3.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. chromatin Source: InterPro
  2. cytoplasm Source: Ensembl
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile. Mice have a mild diabetic phenotype and lower plasma glucagon levels. The overall shape of the islets, the location of the alpha cells in the mantle of the pancreatic islets or proliferation of pancreatic alpha cells are not affected.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9999High mobility group nucleosome-binding domain-containing protein 3PRO_0000232575Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei6 – 61PhosphoserineBy similarity
Modified residuei10 – 101PhosphothreonineBy similarity
Modified residuei78 – 781PhosphoserineBy similarity
Modified residuei93 – 931PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9DCB1.
PaxDbiQ9DCB1.
PRIDEiQ9DCB1.

PTM databases

PhosphoSiteiQ9DCB1.

Expressioni

Tissue specificityi

Expressed in the brain, eye, prostate, thyroid, kidney, testis, glial cells and insulin-producing cells of the Langerhans pancreatic islets. In the brain, expressed in the lateral olfactory tract, anterior commissure, corpus callosum, internal capsule, fornix, stria medullans, optic tract, axon bundles, Purkinje cell layer and granular layer of the cerebellum. In retina, expressed in the nuclei of cells in the inner nuclear layer including amacrine, bipolar and horizontal neurons and in the nuclei of ganglion neurons. Detected at low levels in the liver.6 Publications

Developmental stagei

Transiently expressed in the stroma and endothelium of the cornea at birth. Subsequently expressed in the corneal epithelium and the inner nuclear and ganglion cell layers of the retina. The predominant form in developing ocular tissues is isoform 2, although isoform 1 is also detectable.1 Publication

Gene expression databases

BgeeiQ9DCB1.
CleanExiMM_HMGN3.
GenevestigatoriQ9DCB1.

Interactioni

Subunit structurei

Interacts with the ligand binding domain of the thyroid receptor (TR) (in vitro). Requires the presence of thyroid hormone for its interaction. Interacts with nucleosomes (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9DCB1. 1 interaction.
MINTiMINT-4128604.

Family & Domainsi

Sequence similaritiesi

Belongs to the HMGN family.Curated

Phylogenomic databases

eggNOGiNOG70460.
GeneTreeiENSGT00730000111287.
HOGENOMiHOG000116395.
HOVERGENiHBG073479.
InParanoidiQ9DCB1.
KOiK11301.
OMAiLTKQEPT.
OrthoDBiEOG7HXCTV.
PhylomeDBiQ9DCB1.

Family and domain databases

InterProiIPR000079. HMGN_fam.
[Graphical view]
PANTHERiPTHR23087. PTHR23087. 1 hit.
PfamiPF01101. HMG14_17. 1 hit.
[Graphical view]
PRINTSiPR00925. NONHISHMG17.
SMARTiSM00527. HMG17. 1 hit.
[Graphical view]
PROSITEiPS00355. HMG14_17. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9DCB1-1) [UniParc]FASTAAdd to Basket

Also known as: HMGN3a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPKRKSPENT EGKDGTKLTK QEPTRRSARL SAKPVPPKPE SKPRKTSAKK
60 70 80 90
EPGTKISRGA KGKKEEKQEA GEEGTAPSAN GDTKVEEAQR TESIEKEGE
Length:99
Mass (Da):10,769
Last modified:June 1, 2001 - v1
Checksum:iDE0277EFBE8232CF
GO
Isoform 2 (identifier: Q9DCB1-2) [UniParc]FASTAAdd to Basket

Also known as: HMGN3b

The sequence of this isoform differs from the canonical sequence as follows:
     76-77: AP → EN
     78-99: Missing.

Show »
Length:77
Mass (Da):8,455
Checksum:iB7340DA18071905B
GO
Isoform 3 (identifier: Q9DCB1-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     88-95: AQRTESIE → VLSTNTSH
     96-99: Missing.

Note: No experimental confirmation available.

Show »
Length:95
Mass (Da):10,250
Checksum:i5F67D6D091D77319
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei76 – 772AP → EN in isoform 2. CuratedVSP_017909
Alternative sequencei78 – 9922Missing in isoform 2. CuratedVSP_017910Add
BLAST
Alternative sequencei88 – 958AQRTESIE → VLSTNTSH in isoform 3. 1 PublicationVSP_017911
Alternative sequencei96 – 994Missing in isoform 3. 1 PublicationVSP_017912

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK002970 mRNA. Translation: BAB22485.1.
AK153223 mRNA. Translation: BAE31816.1.
BC005693 mRNA. Translation: AAH05693.1.
BK000004 mRNA. Translation: DAA00393.1.
BK000005 mRNA. Translation: DAA00394.1.
CCDSiCCDS52872.1. [Q9DCB1-1]
CCDS52873.1. [Q9DCB1-2]
RefSeqiNP_080398.1. NM_026122.4. [Q9DCB1-1]
NP_778249.1. NM_175074.2. [Q9DCB1-2]
UniGeneiMm.244426.

Genome annotation databases

EnsembliENSMUST00000161796; ENSMUSP00000125616; ENSMUSG00000066456. [Q9DCB1-2]
ENSMUST00000162246; ENSMUSP00000124278; ENSMUSG00000066456. [Q9DCB1-1]
ENSMUST00000185315; ENSMUSP00000140356; ENSMUSG00000066456. [Q9DCB1-3]
ENSMUST00000190154; ENSMUSP00000140247; ENSMUSG00000066456. [Q9DCB1-3]
GeneIDi94353.
KEGGimmu:94353.
UCSCiuc009qwc.3. mouse. [Q9DCB1-3]
uc009qwd.2. mouse. [Q9DCB1-2]
uc009qwe.2. mouse. [Q9DCB1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK002970 mRNA. Translation: BAB22485.1 .
AK153223 mRNA. Translation: BAE31816.1 .
BC005693 mRNA. Translation: AAH05693.1 .
BK000004 mRNA. Translation: DAA00393.1 .
BK000005 mRNA. Translation: DAA00394.1 .
CCDSi CCDS52872.1. [Q9DCB1-1 ]
CCDS52873.1. [Q9DCB1-2 ]
RefSeqi NP_080398.1. NM_026122.4. [Q9DCB1-1 ]
NP_778249.1. NM_175074.2. [Q9DCB1-2 ]
UniGenei Mm.244426.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9DCB1. 1 interaction.
MINTi MINT-4128604.

PTM databases

PhosphoSitei Q9DCB1.

Proteomic databases

MaxQBi Q9DCB1.
PaxDbi Q9DCB1.
PRIDEi Q9DCB1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000161796 ; ENSMUSP00000125616 ; ENSMUSG00000066456 . [Q9DCB1-2 ]
ENSMUST00000162246 ; ENSMUSP00000124278 ; ENSMUSG00000066456 . [Q9DCB1-1 ]
ENSMUST00000185315 ; ENSMUSP00000140356 ; ENSMUSG00000066456 . [Q9DCB1-3 ]
ENSMUST00000190154 ; ENSMUSP00000140247 ; ENSMUSG00000066456 . [Q9DCB1-3 ]
GeneIDi 94353.
KEGGi mmu:94353.
UCSCi uc009qwc.3. mouse. [Q9DCB1-3 ]
uc009qwd.2. mouse. [Q9DCB1-2 ]
uc009qwe.2. mouse. [Q9DCB1-1 ]

Organism-specific databases

CTDi 9324.
MGIi MGI:2138069. Hmgn3.

Phylogenomic databases

eggNOGi NOG70460.
GeneTreei ENSGT00730000111287.
HOGENOMi HOG000116395.
HOVERGENi HBG073479.
InParanoidi Q9DCB1.
KOi K11301.
OMAi LTKQEPT.
OrthoDBi EOG7HXCTV.
PhylomeDBi Q9DCB1.

Miscellaneous databases

NextBioi 352321.
PROi Q9DCB1.
SOURCEi Search...

Gene expression databases

Bgeei Q9DCB1.
CleanExi MM_HMGN3.
Genevestigatori Q9DCB1.

Family and domain databases

InterProi IPR000079. HMGN_fam.
[Graphical view ]
PANTHERi PTHR23087. PTHR23087. 1 hit.
Pfami PF01101. HMG14_17. 1 hit.
[Graphical view ]
PRINTSi PR00925. NONHISHMG17.
SMARTi SM00527. HMG17. 1 hit.
[Graphical view ]
PROSITEi PS00355. HMG14_17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Bone marrow and Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. "HMGN3a and HMGN3b, two protein isoforms with a tissue-specific expression pattern, expand the cellular repertoire of nucleosome-binding proteins."
    West K.L., Ito Y., Birger Y., Postnikov Y., Shirakawa H., Bustin M.
    J. Biol. Chem. 276:25959-25969(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
  4. "Immunohistochemical localization of the nucleosome-binding protein HMGN3 in mouse brain."
    Ito Y., Bustin M.
    J. Histochem. Cytochem. 50:1273-1275(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  5. "Chromosomal proteins HMGN3a and HMGN3b regulate the expression of glycine transporter 1."
    West K.L., Castellini M.A., Duncan M.K., Bustin M.
    Mol. Cell. Biol. 24:3747-3756(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  6. "Differential expression of the HMGN family of chromatin proteins during ocular development."
    Lucey M.M., Wang Y., Bustin M., Duncan M.K.
    Gene Expr. Patterns 8:433-437(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  7. "The nucleosome binding protein HMGN3 modulates the transcription profile of pancreatic beta cells and affects insulin secretion."
    Ueda T., Furusawa T., Kurahashi T., Tessarollo L., Bustin M.
    Mol. Cell. Biol. 29:5264-5276(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  8. "The nucleosome binding protein HMGN3 is expressed in pancreatic alpha-cells and affects plasma glucagon levels in mice."
    Kurahashi T., Furusawa T., Ueda T., Bustin M.
    J. Cell. Biochem. 109:49-57(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiHMGN3_MOUSE
AccessioniPrimary (citable) accession number: Q9DCB1
Secondary accession number(s): Q7M737, Q99JU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3