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Q9DCB1

- HMGN3_MOUSE

UniProt

Q9DCB1 - HMGN3_MOUSE

Protein

High mobility group nucleosome-binding domain-containing protein 3

Gene

Hmgn3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Binds to nucleosomes, regulating chromatin structure and consequently, chromatin-dependent processes such as transcription, DNA replication and DNA repair. Affects both insulin and glucagon levels and modulates the expression of pancreatic genes involved in insulin secretion. Regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors. Regulates the expression of SLC6A9, a glycine transporter which regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. May play a role in ocular development and astrocyte function.5 Publications

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. chromatin modification Source: UniProtKB-KW
    2. positive regulation of sequence-specific DNA binding transcription factor activity Source: MGI
    3. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    4. regulation of insulin secretion involved in cellular response to glucose stimulus Source: MGI
    5. regulation of transcription from RNA polymerase II promoter Source: MGI

    Keywords - Molecular functioni

    Chromatin regulator

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    High mobility group nucleosome-binding domain-containing protein 3
    Gene namesi
    Name:Hmgn3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:2138069. Hmgn3.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. chromatin Source: InterPro
    2. cytoplasm Source: Ensembl
    3. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mice are viable and fertile. Mice have a mild diabetic phenotype and lower plasma glucagon levels. The overall shape of the islets, the location of the alpha cells in the mantle of the pancreatic islets or proliferation of pancreatic alpha cells are not affected.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 9999High mobility group nucleosome-binding domain-containing protein 3PRO_0000232575Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei6 – 61PhosphoserineBy similarity
    Modified residuei10 – 101PhosphothreonineBy similarity
    Modified residuei78 – 781PhosphoserineBy similarity
    Modified residuei93 – 931PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9DCB1.
    PaxDbiQ9DCB1.
    PRIDEiQ9DCB1.

    PTM databases

    PhosphoSiteiQ9DCB1.

    Expressioni

    Tissue specificityi

    Expressed in the brain, eye, prostate, thyroid, kidney, testis, glial cells and insulin-producing cells of the Langerhans pancreatic islets. In the brain, expressed in the lateral olfactory tract, anterior commissure, corpus callosum, internal capsule, fornix, stria medullans, optic tract, axon bundles, Purkinje cell layer and granular layer of the cerebellum. In retina, expressed in the nuclei of cells in the inner nuclear layer including amacrine, bipolar and horizontal neurons and in the nuclei of ganglion neurons. Detected at low levels in the liver.6 Publications

    Developmental stagei

    Transiently expressed in the stroma and endothelium of the cornea at birth. Subsequently expressed in the corneal epithelium and the inner nuclear and ganglion cell layers of the retina. The predominant form in developing ocular tissues is isoform 2, although isoform 1 is also detectable.1 Publication

    Gene expression databases

    BgeeiQ9DCB1.
    CleanExiMM_HMGN3.
    GenevestigatoriQ9DCB1.

    Interactioni

    Subunit structurei

    Interacts with the ligand binding domain of the thyroid receptor (TR) (in vitro). Requires the presence of thyroid hormone for its interaction. Interacts with nucleosomes By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ9DCB1. 1 interaction.
    MINTiMINT-4128604.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the HMGN family.Curated

    Phylogenomic databases

    eggNOGiNOG70460.
    GeneTreeiENSGT00730000111287.
    HOGENOMiHOG000116395.
    HOVERGENiHBG073479.
    InParanoidiQ9DCB1.
    KOiK11301.
    OMAiLTKQEPT.
    OrthoDBiEOG7HXCTV.
    PhylomeDBiQ9DCB1.

    Family and domain databases

    InterProiIPR000079. HMGN_fam.
    [Graphical view]
    PANTHERiPTHR23087. PTHR23087. 1 hit.
    PfamiPF01101. HMG14_17. 1 hit.
    [Graphical view]
    PRINTSiPR00925. NONHISHMG17.
    SMARTiSM00527. HMG17. 1 hit.
    [Graphical view]
    PROSITEiPS00355. HMG14_17. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9DCB1-1) [UniParc]FASTAAdd to Basket

    Also known as: HMGN3a

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPKRKSPENT EGKDGTKLTK QEPTRRSARL SAKPVPPKPE SKPRKTSAKK   50
    EPGTKISRGA KGKKEEKQEA GEEGTAPSAN GDTKVEEAQR TESIEKEGE 99
    Length:99
    Mass (Da):10,769
    Last modified:June 1, 2001 - v1
    Checksum:iDE0277EFBE8232CF
    GO
    Isoform 2 (identifier: Q9DCB1-2) [UniParc]FASTAAdd to Basket

    Also known as: HMGN3b

    The sequence of this isoform differs from the canonical sequence as follows:
         76-77: AP → EN
         78-99: Missing.

    Show »
    Length:77
    Mass (Da):8,455
    Checksum:iB7340DA18071905B
    GO
    Isoform 3 (identifier: Q9DCB1-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         88-95: AQRTESIE → VLSTNTSH
         96-99: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:95
    Mass (Da):10,250
    Checksum:i5F67D6D091D77319
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei76 – 772AP → EN in isoform 2. CuratedVSP_017909
    Alternative sequencei78 – 9922Missing in isoform 2. CuratedVSP_017910Add
    BLAST
    Alternative sequencei88 – 958AQRTESIE → VLSTNTSH in isoform 3. 1 PublicationVSP_017911
    Alternative sequencei96 – 994Missing in isoform 3. 1 PublicationVSP_017912

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK002970 mRNA. Translation: BAB22485.1.
    AK153223 mRNA. Translation: BAE31816.1.
    BC005693 mRNA. Translation: AAH05693.1.
    BK000004 mRNA. Translation: DAA00393.1.
    BK000005 mRNA. Translation: DAA00394.1.
    CCDSiCCDS52872.1. [Q9DCB1-1]
    CCDS52873.1. [Q9DCB1-2]
    RefSeqiNP_080398.1. NM_026122.4. [Q9DCB1-1]
    NP_778249.1. NM_175074.2. [Q9DCB1-2]
    UniGeneiMm.244426.

    Genome annotation databases

    EnsembliENSMUST00000161796; ENSMUSP00000125616; ENSMUSG00000066456. [Q9DCB1-2]
    ENSMUST00000162246; ENSMUSP00000124278; ENSMUSG00000066456. [Q9DCB1-1]
    GeneIDi94353.
    KEGGimmu:94353.
    UCSCiuc009qwc.3. mouse. [Q9DCB1-3]
    uc009qwd.2. mouse. [Q9DCB1-2]
    uc009qwe.2. mouse. [Q9DCB1-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK002970 mRNA. Translation: BAB22485.1 .
    AK153223 mRNA. Translation: BAE31816.1 .
    BC005693 mRNA. Translation: AAH05693.1 .
    BK000004 mRNA. Translation: DAA00393.1 .
    BK000005 mRNA. Translation: DAA00394.1 .
    CCDSi CCDS52872.1. [Q9DCB1-1 ]
    CCDS52873.1. [Q9DCB1-2 ]
    RefSeqi NP_080398.1. NM_026122.4. [Q9DCB1-1 ]
    NP_778249.1. NM_175074.2. [Q9DCB1-2 ]
    UniGenei Mm.244426.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9DCB1. 1 interaction.
    MINTi MINT-4128604.

    PTM databases

    PhosphoSitei Q9DCB1.

    Proteomic databases

    MaxQBi Q9DCB1.
    PaxDbi Q9DCB1.
    PRIDEi Q9DCB1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000161796 ; ENSMUSP00000125616 ; ENSMUSG00000066456 . [Q9DCB1-2 ]
    ENSMUST00000162246 ; ENSMUSP00000124278 ; ENSMUSG00000066456 . [Q9DCB1-1 ]
    GeneIDi 94353.
    KEGGi mmu:94353.
    UCSCi uc009qwc.3. mouse. [Q9DCB1-3 ]
    uc009qwd.2. mouse. [Q9DCB1-2 ]
    uc009qwe.2. mouse. [Q9DCB1-1 ]

    Organism-specific databases

    CTDi 9324.
    MGIi MGI:2138069. Hmgn3.

    Phylogenomic databases

    eggNOGi NOG70460.
    GeneTreei ENSGT00730000111287.
    HOGENOMi HOG000116395.
    HOVERGENi HBG073479.
    InParanoidi Q9DCB1.
    KOi K11301.
    OMAi LTKQEPT.
    OrthoDBi EOG7HXCTV.
    PhylomeDBi Q9DCB1.

    Miscellaneous databases

    NextBioi 352321.
    PROi Q9DCB1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9DCB1.
    CleanExi MM_HMGN3.
    Genevestigatori Q9DCB1.

    Family and domain databases

    InterProi IPR000079. HMGN_fam.
    [Graphical view ]
    PANTHERi PTHR23087. PTHR23087. 1 hit.
    Pfami PF01101. HMG14_17. 1 hit.
    [Graphical view ]
    PRINTSi PR00925. NONHISHMG17.
    SMARTi SM00527. HMG17. 1 hit.
    [Graphical view ]
    PROSITEi PS00355. HMG14_17. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Bone marrow and Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Strain: FVB/N.
      Tissue: Mammary gland.
    3. "HMGN3a and HMGN3b, two protein isoforms with a tissue-specific expression pattern, expand the cellular repertoire of nucleosome-binding proteins."
      West K.L., Ito Y., Birger Y., Postnikov Y., Shirakawa H., Bustin M.
      J. Biol. Chem. 276:25959-25969(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
    4. "Immunohistochemical localization of the nucleosome-binding protein HMGN3 in mouse brain."
      Ito Y., Bustin M.
      J. Histochem. Cytochem. 50:1273-1275(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    5. "Chromosomal proteins HMGN3a and HMGN3b regulate the expression of glycine transporter 1."
      West K.L., Castellini M.A., Duncan M.K., Bustin M.
      Mol. Cell. Biol. 24:3747-3756(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    6. "Differential expression of the HMGN family of chromatin proteins during ocular development."
      Lucey M.M., Wang Y., Bustin M., Duncan M.K.
      Gene Expr. Patterns 8:433-437(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    7. "The nucleosome binding protein HMGN3 modulates the transcription profile of pancreatic beta cells and affects insulin secretion."
      Ueda T., Furusawa T., Kurahashi T., Tessarollo L., Bustin M.
      Mol. Cell. Biol. 29:5264-5276(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    8. "The nucleosome binding protein HMGN3 is expressed in pancreatic alpha-cells and affects plasma glucagon levels in mice."
      Kurahashi T., Furusawa T., Ueda T., Bustin M.
      J. Cell. Biochem. 109:49-57(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiHMGN3_MOUSE
    AccessioniPrimary (citable) accession number: Q9DCB1
    Secondary accession number(s): Q7M737, Q99JU1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2006
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3