ID   CALY_MOUSE              Reviewed;         226 AA.
AC   Q9DCA7; Q9D1K9;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   24-JAN-2024, entry version 146.
DE   RecName: Full=Neuron-specific vesicular protein calcyon;
GN   Name=Caly; Synonyms=Drd1ip;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=12853145; DOI=10.1016/s0378-1119(03)00564-x;
RA   Dai R., Bergson C.;
RT   "Structure and expression of the murine calcyon gene.";
RL   Gene 311:111-117(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Brain, and Embryo;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=16595675; DOI=10.1074/jbc.m600265200;
RA   Xiao J., Dai R., Negyessy L., Bergson C.;
RT   "Calcyon, a novel partner of clathrin light chain, stimulates clathrin-
RT   mediated endocytosis.";
RL   J. Biol. Chem. 281:15182-15193(2006).
RN   [5]
RP   TRANSGENIC MICE.
RX   PubMed=18295356; DOI=10.1016/j.bbr.2007.12.031;
RA   Trantham-Davidson H., Vazdarjanova A., Dai R., Terry A., Bergson C.;
RT   "Up-regulation of calcyon results in locomotor hyperactivity and reduced
RT   anxiety in mice.";
RL   Behav. Brain Res. 189:244-249(2008).
CC   -!- FUNCTION: Interacts with clathrin light chain A and stimulates clathrin
CC       self-assembly and clathrin-mediated endocytosis. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with CLTA. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}. Cell membrane
CC       {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Most abundant in brain. Also expressed in testis
CC       and ovary and, at much lower levels, in kidney and heart.
CC       {ECO:0000269|PubMed:12853145}.
CC   -!- DISRUPTION PHENOTYPE: Mice display impaired clathrin assembly and
CC       clathrin-mediated endocytosis. Transgenic mice with up-regulated
CC       forebrain expression of Caly display a range of abnormal behaviors
CC       including spontaneous hyperactivity, reduced anxiety and/or impaired
CC       harm avoidance. {ECO:0000269|PubMed:16595675}.
CC   -!- SIMILARITY: Belongs to the NSG family. {ECO:0000305}.
CC   -!- CAUTION: The human ortholog was originally thought to interact with the
CC       D1 dopamine receptor (DRD1) and to play a role in potentiating calcium
CC       ion-dependent signaling but this work was later retracted.
CC       {ECO:0000305}.
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DR   EMBL; AK002979; BAB22492.1; -; mRNA.
DR   EMBL; AK003400; BAB22765.2; -; mRNA.
DR   EMBL; BC049663; AAH49663.1; -; mRNA.
DR   CCDS; CCDS21962.1; -.
DR   RefSeq; NP_001177314.1; NM_001190385.1.
DR   RefSeq; NP_001177315.1; NM_001190386.1.
DR   RefSeq; NP_081045.2; NM_026769.5.
DR   RefSeq; XP_006536300.1; XM_006536237.2.
DR   RefSeq; XP_011248155.1; XM_011249853.2.
DR   AlphaFoldDB; Q9DCA7; -.
DR   BioGRID; 212931; 1.
DR   STRING; 10090.ENSMUSP00000026541; -.
DR   iPTMnet; Q9DCA7; -.
DR   PhosphoSitePlus; Q9DCA7; -.
DR   MaxQB; Q9DCA7; -.
DR   PaxDb; 10090-ENSMUSP00000130779; -.
DR   ProteomicsDB; 265515; -.
DR   Antibodypedia; 32650; 125 antibodies from 27 providers.
DR   DNASU; 68566; -.
DR   Ensembl; ENSMUST00000026541.15; ENSMUSP00000026541.8; ENSMUSG00000025468.16.
DR   Ensembl; ENSMUST00000166758.4; ENSMUSP00000130779.3; ENSMUSG00000025468.16.
DR   Ensembl; ENSMUST00000211044.2; ENSMUSP00000148159.2; ENSMUSG00000025468.16.
DR   Ensembl; ENSMUST00000211283.2; ENSMUSP00000148173.2; ENSMUSG00000025468.16.
DR   GeneID; 68566; -.
DR   KEGG; mmu:68566; -.
DR   UCSC; uc009kgr.1; mouse.
DR   AGR; MGI:1915816; -.
DR   CTD; 50632; -.
DR   MGI; MGI:1915816; Caly.
DR   VEuPathDB; HostDB:ENSMUSG00000025468; -.
DR   eggNOG; ENOG502QW2S; Eukaryota.
DR   GeneTree; ENSGT00390000000483; -.
DR   HOGENOM; CLU_112085_1_0_1; -.
DR   InParanoid; Q9DCA7; -.
DR   OMA; ILKQKHC; -.
DR   OrthoDB; 4831126at2759; -.
DR   PhylomeDB; Q9DCA7; -.
DR   TreeFam; TF332232; -.
DR   BioGRID-ORCS; 68566; 2 hits in 78 CRISPR screens.
DR   PRO; PR:Q9DCA7; -.
DR   Proteomes; UP000000589; Chromosome 7.
DR   RNAct; Q9DCA7; Protein.
DR   Bgee; ENSMUSG00000025468; Expressed in dentate gyrus of hippocampal formation granule cell and 136 other cell types or tissues.
DR   GO; GO:0030424; C:axon; ISO:MGI.
DR   GO; GO:1904115; C:axon cytoplasm; IEA:GOC.
DR   GO; GO:0031410; C:cytoplasmic vesicle; ISS:UniProtKB.
DR   GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005768; C:endosome; IBA:GO_Central.
DR   GO; GO:0098978; C:glutamatergic synapse; IDA:SynGO.
DR   GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR   GO; GO:0098843; C:postsynaptic endocytic zone; ISO:MGI.
DR   GO; GO:0032051; F:clathrin light chain binding; ISS:UniProtKB.
DR   GO; GO:0044877; F:protein-containing complex binding; ISO:MGI.
DR   GO; GO:0008089; P:anterograde axonal transport; ISO:MGI.
DR   GO; GO:0048268; P:clathrin coat assembly; ISS:UniProtKB.
DR   GO; GO:0007212; P:dopamine receptor signaling pathway; IEA:InterPro.
DR   GO; GO:0016197; P:endosomal transport; IBA:GO_Central.
DR   GO; GO:1905445; P:positive regulation of clathrin coat assembly; ISO:MGI.
DR   GO; GO:0045807; P:positive regulation of endocytosis; ISS:UniProtKB.
DR   GO; GO:2001019; P:positive regulation of retrograde axon cargo transport; ISO:MGI.
DR   GO; GO:0098884; P:postsynaptic neurotransmitter receptor internalization; IDA:SynGO.
DR   InterPro; IPR009431; NSG.
DR   PANTHER; PTHR28546:SF1; NEURON-SPECIFIC VESICULAR PROTEIN CALCYON; 1.
DR   PANTHER; PTHR28546; NEURONAL VESICLE TRAFFICKING-ASSOCIATED PROTEIN 2-RELATED; 1.
DR   Pfam; PF06387; Calcyon; 1.
DR   PIRSF; PIRSF002383; Calcyon; 1.
DR   Genevisible; Q9DCA7; MM.
PE   2: Evidence at transcript level;
KW   Cell membrane; Cytoplasmic vesicle; Endocytosis; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..226
FT                   /note="Neuron-specific vesicular protein calcyon"
FT                   /id="PRO_0000164369"
FT   TOPO_DOM        1..88
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        110..226
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          177..226
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        203..217
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   226 AA;  24580 MW;  AFF880F4292ADC98 CRC64;
     MVKLGCSFSG KPGKEAGDQD GAAMDSVPLI SPLDVSQLQP SFSDQVVINT QTEYQLTSAD
     QPKKFADLEG QRLACSHSEE GRRLPTARMI AFAMALLGCV LIMYKAIWYD QFTCPDGFLL
     RHKICTPLTL EMYYTEMDPE RHRSILAAIG AYPLSRKHGT EMPAVWGNNY RTAKEEHKGT
     TPAAMAVSTA AAAAAAEGTE PSGKSLDTRE KEDPQKAEGV PSQPPK
//