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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

Gene

Ndufs7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi100 – 1001Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi164 – 1641Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi194 – 1941Iron-sulfur (4Fe-4S)Sequence Analysis

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  4. quinone binding Source: InterPro

GO - Biological processi

  1. mitochondrial respiratory chain complex I assembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiREACT_262523. Respiratory electron transport.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-20kD
Short name:
CI-20kD
NADH-ubiquinone oxidoreductase 20 kDa subunit
Gene namesi
Name:Ndufs7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:1922656. Ndufs7.

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrial inner membrane Source: MGI
  2. mitochondrial respiratory chain complex I Source: UniProtKB
  3. mitochondrion Source: MGI
  4. neuronal cell body Source: Ensembl
  5. neuron projection Source: Ensembl
  6. synaptic membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3535MitochondrionBy similarityAdd
BLAST
Chaini36 – 224189NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrialPRO_0000020028Add
BLAST

Proteomic databases

MaxQBiQ9DC70.
PaxDbiQ9DC70.
PRIDEiQ9DC70.

PTM databases

PhosphoSiteiQ9DC70.

Expressioni

Gene expression databases

BgeeiQ9DC70.
CleanExiMM_NDUFS7.
GenevestigatoriQ9DC70.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.By similarity

Protein-protein interaction databases

BioGridi217459. 1 interaction.
IntActiQ9DC70. 1 interaction.
MINTiMINT-1861354.
STRINGi10090.ENSMUSP00000020361.

Structurei

3D structure databases

ProteinModelPortaliQ9DC70.
SMRiQ9DC70. Positions 70-213.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 20 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0377.
GeneTreeiENSGT00390000006565.
HOGENOMiHOG000228249.
HOVERGENiHBG001576.
InParanoidiQ9DC70.
KOiK03940.
OMAiMAEWSLA.
OrthoDBiEOG7C8GJ9.
PhylomeDBiQ9DC70.
TreeFamiTF312859.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
HAMAPiMF_01356. NDH1_NuoB.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DC70-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAALAAPGLL SVRILGLRTA QVQLRRVHQS VATEGPSPSP SPSLSSTQSA
60 70 80 90 100
VSKAGAGAVV PKLSHLPRSR AEYVVTKLDD LINWARRSSL WPMTFGLACC
110 120 130 140 150
AVEMMHMAAP RYDMDRFGVV FRASPRQADV MIVAGTLTNK MAPALRKVYD
160 170 180 190 200
QMPEPRYVVS MGSCANGGGY YHYSYSVVRG CDRIVPVDIY VPGCPPTAEA
210 220
LLYGILQLQR KIKREQKLKI WYRR
Length:224
Mass (Da):24,683
Last modified:June 1, 2001 - v1
Checksum:i234F3FF0ABC36A92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003132 mRNA. Translation: BAB22592.1.
BC013503 mRNA. Translation: AAH13503.1.
CCDSiCCDS24015.1.
RefSeqiNP_083548.1. NM_029272.3.
UniGeneiMm.28712.

Genome annotation databases

EnsembliENSMUST00000020361; ENSMUSP00000020361; ENSMUSG00000020153.
ENSMUST00000105364; ENSMUSP00000101003; ENSMUSG00000020153.
GeneIDi75406.
KEGGimmu:75406.
UCSCiuc007gci.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003132 mRNA. Translation: BAB22592.1.
BC013503 mRNA. Translation: AAH13503.1.
CCDSiCCDS24015.1.
RefSeqiNP_083548.1. NM_029272.3.
UniGeneiMm.28712.

3D structure databases

ProteinModelPortaliQ9DC70.
SMRiQ9DC70. Positions 70-213.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217459. 1 interaction.
IntActiQ9DC70. 1 interaction.
MINTiMINT-1861354.
STRINGi10090.ENSMUSP00000020361.

PTM databases

PhosphoSiteiQ9DC70.

Proteomic databases

MaxQBiQ9DC70.
PaxDbiQ9DC70.
PRIDEiQ9DC70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020361; ENSMUSP00000020361; ENSMUSG00000020153.
ENSMUST00000105364; ENSMUSP00000101003; ENSMUSG00000020153.
GeneIDi75406.
KEGGimmu:75406.
UCSCiuc007gci.1. mouse.

Organism-specific databases

CTDi374291.
MGIiMGI:1922656. Ndufs7.

Phylogenomic databases

eggNOGiCOG0377.
GeneTreeiENSGT00390000006565.
HOGENOMiHOG000228249.
HOVERGENiHBG001576.
InParanoidiQ9DC70.
KOiK03940.
OMAiMAEWSLA.
OrthoDBiEOG7C8GJ9.
PhylomeDBiQ9DC70.
TreeFamiTF312859.

Enzyme and pathway databases

ReactomeiREACT_262523. Respiratory electron transport.

Miscellaneous databases

NextBioi342928.
PROiQ9DC70.
SOURCEiSearch...

Gene expression databases

BgeeiQ9DC70.
CleanExiMM_NDUFS7.
GenevestigatoriQ9DC70.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
HAMAPiMF_01356. NDH1_NuoB.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Heart.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon.
  3. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 78-86; 127-140 AND 157-179, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiNDUS7_MOUSE
AccessioniPrimary (citable) accession number: Q9DC70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 2001
Last modified: January 7, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.