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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

Gene

Ndufs7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi100Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi164Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi194Iron-sulfur (4Fe-4S)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.
R-MMU-6799198. Complex I biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-20kD
Short name:
CI-20kD
NADH-ubiquinone oxidoreductase 20 kDa subunit
Gene namesi
Name:Ndufs7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1922656. Ndufs7.

Subcellular locationi

  • Mitochondrion By similarity

GO - Cellular componenti

  • mitochondrial inner membrane Source: MGI
  • mitochondrial respiratory chain complex I Source: UniProtKB
  • mitochondrion Source: MGI
  • neuronal cell body Source: Ensembl
  • neuron projection Source: Ensembl
  • synaptic membrane Source: Ensembl

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35MitochondrionBy similarityAdd BLAST35
ChainiPRO_000002002836 – 224NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrialAdd BLAST189

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39PhosphoserineCombined sources1
Modified residuei41PhosphoserineCombined sources1

Post-translational modificationi

Hydroxylated ar Arg-111 by NDUFAF5 early in the pathway of assembly of complex I, before the formation of the juncture between peripheral and membrane arms.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9DC70.
MaxQBiQ9DC70.
PaxDbiQ9DC70.
PeptideAtlasiQ9DC70.
PRIDEiQ9DC70.

PTM databases

iPTMnetiQ9DC70.
PhosphoSitePlusiQ9DC70.
SwissPalmiQ9DC70.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020153.
CleanExiMM_NDUFS7.
GenevisibleiQ9DC70. MM.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi217459. 1 interactor.
IntActiQ9DC70. 1 interactor.
MINTiMINT-1861354.
STRINGi10090.ENSMUSP00000020361.

Structurei

3D structure databases

ProteinModelPortaliQ9DC70.
SMRiQ9DC70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 20 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1687. Eukaryota.
COG0377. LUCA.
GeneTreeiENSGT00390000006565.
HOGENOMiHOG000228249.
HOVERGENiHBG001576.
InParanoidiQ9DC70.
KOiK03940.
OMAiMAEWSLA.
OrthoDBiEOG091G0ST7.
PhylomeDBiQ9DC70.
TreeFamiTF312859.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
HAMAPiMF_01356. NDH1_NuoB. 1 hit.
InterProiView protein in InterPro
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
PfamiView protein in Pfam
PF01058. Oxidored_q6. 1 hit.
TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
PROSITEiView protein in PROSITE
PS01150. COMPLEX1_20K. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DC70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALAAPGLL SVRILGLRTA QVQLRRVHQS VATEGPSPSP SPSLSSTQSA
60 70 80 90 100
VSKAGAGAVV PKLSHLPRSR AEYVVTKLDD LINWARRSSL WPMTFGLACC
110 120 130 140 150
AVEMMHMAAP RYDMDRFGVV FRASPRQADV MIVAGTLTNK MAPALRKVYD
160 170 180 190 200
QMPEPRYVVS MGSCANGGGY YHYSYSVVRG CDRIVPVDIY VPGCPPTAEA
210 220
LLYGILQLQR KIKREQKLKI WYRR
Length:224
Mass (Da):24,683
Last modified:June 1, 2001 - v1
Checksum:i234F3FF0ABC36A92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003132 mRNA. Translation: BAB22592.1.
BC013503 mRNA. Translation: AAH13503.1.
CCDSiCCDS24015.1.
RefSeqiNP_083548.1. NM_029272.3.
UniGeneiMm.28712.

Genome annotation databases

EnsembliENSMUST00000020361; ENSMUSP00000020361; ENSMUSG00000020153.
ENSMUST00000105364; ENSMUSP00000101003; ENSMUSG00000020153.
GeneIDi75406.
KEGGimmu:75406.
UCSCiuc007gci.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003132 mRNA. Translation: BAB22592.1.
BC013503 mRNA. Translation: AAH13503.1.
CCDSiCCDS24015.1.
RefSeqiNP_083548.1. NM_029272.3.
UniGeneiMm.28712.

3D structure databases

ProteinModelPortaliQ9DC70.
SMRiQ9DC70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217459. 1 interactor.
IntActiQ9DC70. 1 interactor.
MINTiMINT-1861354.
STRINGi10090.ENSMUSP00000020361.

PTM databases

iPTMnetiQ9DC70.
PhosphoSitePlusiQ9DC70.
SwissPalmiQ9DC70.

Proteomic databases

EPDiQ9DC70.
MaxQBiQ9DC70.
PaxDbiQ9DC70.
PeptideAtlasiQ9DC70.
PRIDEiQ9DC70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020361; ENSMUSP00000020361; ENSMUSG00000020153.
ENSMUST00000105364; ENSMUSP00000101003; ENSMUSG00000020153.
GeneIDi75406.
KEGGimmu:75406.
UCSCiuc007gci.1. mouse.

Organism-specific databases

CTDi374291.
MGIiMGI:1922656. Ndufs7.

Phylogenomic databases

eggNOGiKOG1687. Eukaryota.
COG0377. LUCA.
GeneTreeiENSGT00390000006565.
HOGENOMiHOG000228249.
HOVERGENiHBG001576.
InParanoidiQ9DC70.
KOiK03940.
OMAiMAEWSLA.
OrthoDBiEOG091G0ST7.
PhylomeDBiQ9DC70.
TreeFamiTF312859.

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.
R-MMU-6799198. Complex I biogenesis.

Miscellaneous databases

PROiPR:Q9DC70.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020153.
CleanExiMM_NDUFS7.
GenevisibleiQ9DC70. MM.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
HAMAPiMF_01356. NDH1_NuoB. 1 hit.
InterProiView protein in InterPro
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
PfamiView protein in Pfam
PF01058. Oxidored_q6. 1 hit.
TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
PROSITEiView protein in PROSITE
PS01150. COMPLEX1_20K. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNDUS7_MOUSE
AccessioniPrimary (citable) accession number: Q9DC70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 2001
Last modified: May 10, 2017
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.