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Protein

F-box only protein 3

Gene

Fbxo3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 3
Gene namesi
Name:Fbxo3
Synonyms:Fba, Fbx3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1929084. Fbxo3.

Subcellular locationi

  • Nucleus By similarity

  • Note: Colocalizes with PML at the peripheries of nuclear bodies.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 480480F-box only protein 3PRO_0000119878Add
BLAST

Proteomic databases

EPDiQ9DC63.
MaxQBiQ9DC63.
PaxDbiQ9DC63.
PeptideAtlasiQ9DC63.
PRIDEiQ9DC63.

PTM databases

iPTMnetiQ9DC63.
PhosphoSiteiQ9DC63.

Expressioni

Gene expression databases

BgeeiQ9DC63.
CleanExiMM_FBXO3.
ExpressionAtlasiQ9DC63. baseline and differential.
GenevisibleiQ9DC63. MM.

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex consisting of FBXO3, SKP1, CUL1 and RBX1. Interacts with PML, interaction is direct and takes place either alone or within the SCF complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi208300. 7 interactions.
STRINGi10090.ENSMUSP00000028603.

Structurei

3D structure databases

ProteinModelPortaliQ9DC63.
SMRiQ9DC63. Positions 284-380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 5647F-boxPROSITE-ProRule annotationAdd
BLAST
Domaini278 – 408131ApaGPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi414 – 46047Asp/Glu-rich (highly acidic)Add
BLAST
Compositional biasi462 – 4654Poly-Arg
Compositional biasi472 – 4754Poly-Arg

Sequence similaritiesi

Contains 1 apaG domain.PROSITE-ProRule annotation
Contains 1 F-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4408. Eukaryota.
COG2967. LUCA.
GeneTreeiENSGT00390000009225.
HOVERGENiHBG005647.
InParanoidiQ9DC63.
KOiK10290.
OMAiDLGRYIQ.
OrthoDBiEOG7GJ6CS.
PhylomeDBiQ9DC63.
TreeFamiTF329795.

Family and domain databases

Gene3Di2.60.40.1470. 1 hit.
InterProiIPR007474. ApaG_domain.
IPR001810. F-box_dom.
IPR018958. SMI1/KNR4-like_dom.
[Graphical view]
PfamiPF04379. DUF525. 1 hit.
PF12937. F-box-like. 1 hit.
PF09346. SMI1_KNR4. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00860. SMI1_KNR4. 1 hit.
[Graphical view]
SUPFAMiSSF110069. SSF110069. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS51087. APAG. 1 hit.
PS50181. FBOX. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9DC63-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAVEAETGL LTLESLPTDP LLLILSFVDY RDLINCCYVS RRLSQLSTHD
60 70 80 90 100
PLWRRHCKKY WLISEEEKAG KSQCWRSLFI ETYSDVGRYI DHYAAIKKAW
110 120 130 140 150
RDLKKYLEPR CPRMVLSLKE GAREEDLDAV EAQIGCKLPD DYRCSYRIHN
160 170 180 190 200
GQKLVVPGLL GSMALSNHYR SEDLLDVDTA AGGFQQRQGL KYCLPLTFCI
210 220 230 240 250
HTGLSQYIAV EAAEGRNKNE VFYQCPDQMA RNPAAIDMFI IGATFTDWFT
260 270 280 290 300
SYVNNVVSGG FPIIRDQIFR YIHDPECVAT TGDITVSVST SFLPELSSVH
310 320 330 340 350
PPHYFFTYRI RIEMSRDALP EKACQLDSRY WRITNAKGDV EEVQGPGVVG
360 370 380 390 400
EFPIISPGRI YEYTSCTTFS TTSGYMEGYY TFHFLYFKDK VFNVAIPRFH
410 420 430 440 450
MACPTFRVSI ARLEMGPDEY EEMEEEAEEE EEEENDDSAD MDESDESDAD
460 470 480
ENESDEGEGE ARRRRVFDVP IRRRRCSRLF
Length:480
Mass (Da):55,227
Last modified:June 1, 2001 - v1
Checksum:i9AD9565076A67787
GO
Isoform 2 (identifier: Q9DC63-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     414-415: EM → VS
     416-480: Missing.

Note: No experimental confirmation available.
Show »
Length:415
Mass (Da):47,453
Checksum:i2CF5277BD0C4DE2A
GO
Isoform 3 (identifier: Q9DC63-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     36-40: Missing.
     414-415: EM → VS
     416-480: Missing.

Note: No experimental confirmation available.
Show »
Length:410
Mass (Da):46,898
Checksum:i49D736A82FF9BDDF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti63 – 631I → M in BAB31793 (PubMed:16141072).Curated
Sequence conflicti86 – 861V → M in BAC34118 (PubMed:16141072).Curated
Sequence conflicti214 – 2141E → V in BAC25719 (PubMed:16141072).Curated
Sequence conflicti228 – 2281Q → K in BAB31793 (PubMed:16141072).Curated
Sequence conflicti262 – 2621P → H in BAE30746 (PubMed:16141072).Curated
Sequence conflicti262 – 2621P → H in BAE30138 (PubMed:16141072).Curated
Sequence conflicti458 – 4581E → A in BAE30746 (PubMed:16141072).Curated
Sequence conflicti458 – 4581E → A in BAE30138 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei36 – 405Missing in isoform 3. CuratedVSP_039615
Alternative sequencei414 – 4152EM → VS in isoform 2 and isoform 3. 1 PublicationVSP_039616
Alternative sequencei416 – 48065Missing in isoform 2 and isoform 3. 1 PublicationVSP_039617Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233226 mRNA. Translation: AAF67156.1.
AK004544 mRNA. Translation: BAB23360.1.
AK019550 mRNA. Translation: BAB31793.1.
AK028048 mRNA. Translation: BAC25719.1.
AK031428 mRNA. Translation: BAC27398.1.
AK049870 mRNA. Translation: BAC33965.1.
AK050195 mRNA. Translation: BAC34118.1.
AK151130 mRNA. Translation: BAE30138.1.
AK151857 mRNA. Translation: BAE30746.1.
AK152780 mRNA. Translation: BAE31491.1.
AK167075 mRNA. Translation: BAE39235.1.
BX640578 Genomic DNA. Translation: CAM18978.1.
BX640578 Genomic DNA. Translation: CAM18980.1.
BX640578 Genomic DNA. Translation: CAM18981.1.
CH466519 Genomic DNA. Translation: EDL27711.1.
CH466519 Genomic DNA. Translation: EDL27712.1.
BC010212 mRNA. Translation: AAH10212.2.
BC058253 mRNA. Translation: AAH58253.1.
CCDSiCCDS16484.1. [Q9DC63-1]
CCDS16485.1. [Q9DC63-2]
RefSeqiNP_065618.1. NM_020593.2. [Q9DC63-2]
NP_997598.1. NM_212433.1. [Q9DC63-1]
UniGeneiMm.143768.

Genome annotation databases

EnsembliENSMUST00000028603; ENSMUSP00000028603; ENSMUSG00000027180. [Q9DC63-1]
ENSMUST00000102565; ENSMUSP00000099625; ENSMUSG00000027180. [Q9DC63-2]
ENSMUST00000111135; ENSMUSP00000106765; ENSMUSG00000027180. [Q9DC63-3]
GeneIDi57443.
KEGGimmu:57443.
UCSCiuc008lji.1. mouse. [Q9DC63-2]
uc008ljk.1. mouse. [Q9DC63-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233226 mRNA. Translation: AAF67156.1.
AK004544 mRNA. Translation: BAB23360.1.
AK019550 mRNA. Translation: BAB31793.1.
AK028048 mRNA. Translation: BAC25719.1.
AK031428 mRNA. Translation: BAC27398.1.
AK049870 mRNA. Translation: BAC33965.1.
AK050195 mRNA. Translation: BAC34118.1.
AK151130 mRNA. Translation: BAE30138.1.
AK151857 mRNA. Translation: BAE30746.1.
AK152780 mRNA. Translation: BAE31491.1.
AK167075 mRNA. Translation: BAE39235.1.
BX640578 Genomic DNA. Translation: CAM18978.1.
BX640578 Genomic DNA. Translation: CAM18980.1.
BX640578 Genomic DNA. Translation: CAM18981.1.
CH466519 Genomic DNA. Translation: EDL27711.1.
CH466519 Genomic DNA. Translation: EDL27712.1.
BC010212 mRNA. Translation: AAH10212.2.
BC058253 mRNA. Translation: AAH58253.1.
CCDSiCCDS16484.1. [Q9DC63-1]
CCDS16485.1. [Q9DC63-2]
RefSeqiNP_065618.1. NM_020593.2. [Q9DC63-2]
NP_997598.1. NM_212433.1. [Q9DC63-1]
UniGeneiMm.143768.

3D structure databases

ProteinModelPortaliQ9DC63.
SMRiQ9DC63. Positions 284-380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208300. 7 interactions.
STRINGi10090.ENSMUSP00000028603.

PTM databases

iPTMnetiQ9DC63.
PhosphoSiteiQ9DC63.

Proteomic databases

EPDiQ9DC63.
MaxQBiQ9DC63.
PaxDbiQ9DC63.
PeptideAtlasiQ9DC63.
PRIDEiQ9DC63.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028603; ENSMUSP00000028603; ENSMUSG00000027180. [Q9DC63-1]
ENSMUST00000102565; ENSMUSP00000099625; ENSMUSG00000027180. [Q9DC63-2]
ENSMUST00000111135; ENSMUSP00000106765; ENSMUSG00000027180. [Q9DC63-3]
GeneIDi57443.
KEGGimmu:57443.
UCSCiuc008lji.1. mouse. [Q9DC63-2]
uc008ljk.1. mouse. [Q9DC63-1]

Organism-specific databases

CTDi26273.
MGIiMGI:1929084. Fbxo3.

Phylogenomic databases

eggNOGiKOG4408. Eukaryota.
COG2967. LUCA.
GeneTreeiENSGT00390000009225.
HOVERGENiHBG005647.
InParanoidiQ9DC63.
KOiK10290.
OMAiDLGRYIQ.
OrthoDBiEOG7GJ6CS.
PhylomeDBiQ9DC63.
TreeFamiTF329795.

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiFbxo3. mouse.
PROiQ9DC63.
SOURCEiSearch...

Gene expression databases

BgeeiQ9DC63.
CleanExiMM_FBXO3.
ExpressionAtlasiQ9DC63. baseline and differential.
GenevisibleiQ9DC63. MM.

Family and domain databases

Gene3Di2.60.40.1470. 1 hit.
InterProiIPR007474. ApaG_domain.
IPR001810. F-box_dom.
IPR018958. SMI1/KNR4-like_dom.
[Graphical view]
PfamiPF04379. DUF525. 1 hit.
PF12937. F-box-like. 1 hit.
PF09346. SMI1_KNR4. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00860. SMI1_KNR4. 1 hit.
[Graphical view]
SUPFAMiSSF110069. SSF110069. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS51087. APAG. 1 hit.
PS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and expression analysis of new members of the mammalian F-box protein family."
    Ilyin G.P., Rialland M., Pigeon C., Guguen-Guillouzo C.
    Genomics 67:40-47(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Liver.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Bone marrow, Hippocampus, Liver, Lung and Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Eye and Mammary tumor.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen and Testis.

Entry informationi

Entry nameiFBX3_MOUSE
AccessioniPrimary (citable) accession number: Q9DC63
Secondary accession number(s): A2BHN5
, A2BHN7, Q3TKB0, Q3U9C1, Q8C7I0, Q8CEJ0, Q91VI9, Q9D2J5, Q9JIE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.