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Protein

F-box only protein 3

Gene

Fbxo3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 3
Gene namesi
Name:Fbxo3
Synonyms:Fba, Fbx3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1929084. Fbxo3.

Subcellular locationi

  • Nucleus By similarity

  • Note: Colocalizes with PML at the peripheries of nuclear bodies.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198781 – 480F-box only protein 3Add BLAST480

Proteomic databases

PaxDbiQ9DC63.
PeptideAtlasiQ9DC63.
PRIDEiQ9DC63.

PTM databases

iPTMnetiQ9DC63.
PhosphoSitePlusiQ9DC63.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027180.
CleanExiMM_FBXO3.
ExpressionAtlasiQ9DC63. baseline and differential.
GenevisibleiQ9DC63. MM.

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex consisting of FBXO3, SKP1, CUL1 and RBX1. Interacts with PML, interaction is direct and takes place either alone or within the SCF complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi208300. 7 interactors.
STRINGi10090.ENSMUSP00000028603.

Structurei

3D structure databases

ProteinModelPortaliQ9DC63.
SMRiQ9DC63.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 56F-boxPROSITE-ProRule annotationAdd BLAST47
Domaini278 – 408ApaGPROSITE-ProRule annotationAdd BLAST131

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi414 – 460Asp/Glu-rich (highly acidic)Add BLAST47
Compositional biasi462 – 465Poly-Arg4
Compositional biasi472 – 475Poly-Arg4

Sequence similaritiesi

Contains 1 apaG domain.PROSITE-ProRule annotation
Contains 1 F-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4408. Eukaryota.
COG2967. LUCA.
GeneTreeiENSGT00390000009225.
HOVERGENiHBG005647.
InParanoidiQ9DC63.
KOiK10290.
OMAiDLGRYIQ.
OrthoDBiEOG091G08GB.
PhylomeDBiQ9DC63.
TreeFamiTF329795.

Family and domain databases

Gene3Di2.60.40.1470. 1 hit.
InterProiIPR007474. ApaG_domain.
IPR001810. F-box_dom.
IPR018958. SMI1/KNR4-like_dom.
[Graphical view]
PfamiPF04379. DUF525. 1 hit.
PF12937. F-box-like. 1 hit.
PF09346. SMI1_KNR4. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00860. SMI1_KNR4. 1 hit.
[Graphical view]
SUPFAMiSSF110069. SSF110069. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS51087. APAG. 1 hit.
PS50181. FBOX. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9DC63-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAVEAETGL LTLESLPTDP LLLILSFVDY RDLINCCYVS RRLSQLSTHD
60 70 80 90 100
PLWRRHCKKY WLISEEEKAG KSQCWRSLFI ETYSDVGRYI DHYAAIKKAW
110 120 130 140 150
RDLKKYLEPR CPRMVLSLKE GAREEDLDAV EAQIGCKLPD DYRCSYRIHN
160 170 180 190 200
GQKLVVPGLL GSMALSNHYR SEDLLDVDTA AGGFQQRQGL KYCLPLTFCI
210 220 230 240 250
HTGLSQYIAV EAAEGRNKNE VFYQCPDQMA RNPAAIDMFI IGATFTDWFT
260 270 280 290 300
SYVNNVVSGG FPIIRDQIFR YIHDPECVAT TGDITVSVST SFLPELSSVH
310 320 330 340 350
PPHYFFTYRI RIEMSRDALP EKACQLDSRY WRITNAKGDV EEVQGPGVVG
360 370 380 390 400
EFPIISPGRI YEYTSCTTFS TTSGYMEGYY TFHFLYFKDK VFNVAIPRFH
410 420 430 440 450
MACPTFRVSI ARLEMGPDEY EEMEEEAEEE EEEENDDSAD MDESDESDAD
460 470 480
ENESDEGEGE ARRRRVFDVP IRRRRCSRLF
Length:480
Mass (Da):55,227
Last modified:June 1, 2001 - v1
Checksum:i9AD9565076A67787
GO
Isoform 2 (identifier: Q9DC63-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     414-415: EM → VS
     416-480: Missing.

Note: No experimental confirmation available.
Show »
Length:415
Mass (Da):47,453
Checksum:i2CF5277BD0C4DE2A
GO
Isoform 3 (identifier: Q9DC63-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     36-40: Missing.
     414-415: EM → VS
     416-480: Missing.

Note: No experimental confirmation available.
Show »
Length:410
Mass (Da):46,898
Checksum:i49D736A82FF9BDDF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti63I → M in BAB31793 (PubMed:16141072).Curated1
Sequence conflicti86V → M in BAC34118 (PubMed:16141072).Curated1
Sequence conflicti214E → V in BAC25719 (PubMed:16141072).Curated1
Sequence conflicti228Q → K in BAB31793 (PubMed:16141072).Curated1
Sequence conflicti262P → H in BAE30746 (PubMed:16141072).Curated1
Sequence conflicti262P → H in BAE30138 (PubMed:16141072).Curated1
Sequence conflicti458E → A in BAE30746 (PubMed:16141072).Curated1
Sequence conflicti458E → A in BAE30138 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03961536 – 40Missing in isoform 3. Curated5
Alternative sequenceiVSP_039616414 – 415EM → VS in isoform 2 and isoform 3. 1 Publication2
Alternative sequenceiVSP_039617416 – 480Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST65

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233226 mRNA. Translation: AAF67156.1.
AK004544 mRNA. Translation: BAB23360.1.
AK019550 mRNA. Translation: BAB31793.1.
AK028048 mRNA. Translation: BAC25719.1.
AK031428 mRNA. Translation: BAC27398.1.
AK049870 mRNA. Translation: BAC33965.1.
AK050195 mRNA. Translation: BAC34118.1.
AK151130 mRNA. Translation: BAE30138.1.
AK151857 mRNA. Translation: BAE30746.1.
AK152780 mRNA. Translation: BAE31491.1.
AK167075 mRNA. Translation: BAE39235.1.
BX640578 Genomic DNA. Translation: CAM18978.1.
BX640578 Genomic DNA. Translation: CAM18980.1.
BX640578 Genomic DNA. Translation: CAM18981.1.
CH466519 Genomic DNA. Translation: EDL27711.1.
CH466519 Genomic DNA. Translation: EDL27712.1.
BC010212 mRNA. Translation: AAH10212.2.
BC058253 mRNA. Translation: AAH58253.1.
CCDSiCCDS16484.1. [Q9DC63-1]
CCDS16485.1. [Q9DC63-2]
RefSeqiNP_065618.1. NM_020593.2. [Q9DC63-2]
NP_997598.1. NM_212433.1. [Q9DC63-1]
UniGeneiMm.143768.

Genome annotation databases

EnsembliENSMUST00000028603; ENSMUSP00000028603; ENSMUSG00000027180. [Q9DC63-1]
ENSMUST00000102565; ENSMUSP00000099625; ENSMUSG00000027180. [Q9DC63-2]
ENSMUST00000111135; ENSMUSP00000106765; ENSMUSG00000027180. [Q9DC63-3]
GeneIDi57443.
KEGGimmu:57443.
UCSCiuc008lji.1. mouse. [Q9DC63-2]
uc008ljk.1. mouse. [Q9DC63-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233226 mRNA. Translation: AAF67156.1.
AK004544 mRNA. Translation: BAB23360.1.
AK019550 mRNA. Translation: BAB31793.1.
AK028048 mRNA. Translation: BAC25719.1.
AK031428 mRNA. Translation: BAC27398.1.
AK049870 mRNA. Translation: BAC33965.1.
AK050195 mRNA. Translation: BAC34118.1.
AK151130 mRNA. Translation: BAE30138.1.
AK151857 mRNA. Translation: BAE30746.1.
AK152780 mRNA. Translation: BAE31491.1.
AK167075 mRNA. Translation: BAE39235.1.
BX640578 Genomic DNA. Translation: CAM18978.1.
BX640578 Genomic DNA. Translation: CAM18980.1.
BX640578 Genomic DNA. Translation: CAM18981.1.
CH466519 Genomic DNA. Translation: EDL27711.1.
CH466519 Genomic DNA. Translation: EDL27712.1.
BC010212 mRNA. Translation: AAH10212.2.
BC058253 mRNA. Translation: AAH58253.1.
CCDSiCCDS16484.1. [Q9DC63-1]
CCDS16485.1. [Q9DC63-2]
RefSeqiNP_065618.1. NM_020593.2. [Q9DC63-2]
NP_997598.1. NM_212433.1. [Q9DC63-1]
UniGeneiMm.143768.

3D structure databases

ProteinModelPortaliQ9DC63.
SMRiQ9DC63.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208300. 7 interactors.
STRINGi10090.ENSMUSP00000028603.

PTM databases

iPTMnetiQ9DC63.
PhosphoSitePlusiQ9DC63.

Proteomic databases

PaxDbiQ9DC63.
PeptideAtlasiQ9DC63.
PRIDEiQ9DC63.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028603; ENSMUSP00000028603; ENSMUSG00000027180. [Q9DC63-1]
ENSMUST00000102565; ENSMUSP00000099625; ENSMUSG00000027180. [Q9DC63-2]
ENSMUST00000111135; ENSMUSP00000106765; ENSMUSG00000027180. [Q9DC63-3]
GeneIDi57443.
KEGGimmu:57443.
UCSCiuc008lji.1. mouse. [Q9DC63-2]
uc008ljk.1. mouse. [Q9DC63-1]

Organism-specific databases

CTDi26273.
MGIiMGI:1929084. Fbxo3.

Phylogenomic databases

eggNOGiKOG4408. Eukaryota.
COG2967. LUCA.
GeneTreeiENSGT00390000009225.
HOVERGENiHBG005647.
InParanoidiQ9DC63.
KOiK10290.
OMAiDLGRYIQ.
OrthoDBiEOG091G08GB.
PhylomeDBiQ9DC63.
TreeFamiTF329795.

Miscellaneous databases

ChiTaRSiFbxo3. mouse.
PROiQ9DC63.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027180.
CleanExiMM_FBXO3.
ExpressionAtlasiQ9DC63. baseline and differential.
GenevisibleiQ9DC63. MM.

Family and domain databases

Gene3Di2.60.40.1470. 1 hit.
InterProiIPR007474. ApaG_domain.
IPR001810. F-box_dom.
IPR018958. SMI1/KNR4-like_dom.
[Graphical view]
PfamiPF04379. DUF525. 1 hit.
PF12937. F-box-like. 1 hit.
PF09346. SMI1_KNR4. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00860. SMI1_KNR4. 1 hit.
[Graphical view]
SUPFAMiSSF110069. SSF110069. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS51087. APAG. 1 hit.
PS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFBX3_MOUSE
AccessioniPrimary (citable) accession number: Q9DC63
Secondary accession number(s): A2BHN5
, A2BHN7, Q3TKB0, Q3U9C1, Q8C7I0, Q8CEJ0, Q91VI9, Q9D2J5, Q9JIE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.