Q9DC58 (DRAM1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA damage-regulated autophagy modulator protein 1

Alternative name(s):
Damage-regulated autophagy modulator

Gene names

Name:	Dram1
Synonyms:	Dram

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated autophagy By similarity.
Subcellular location	Lysosome membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the DRAM/TMEM150 family.

Ontologies

Keywords
Biological process	Apoptosis Autophagy
Cellular component	Lysosome Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW autophagy Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9DC58-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9DC58-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-106: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	DNA damage-regulated autophagy modulator protein 1		PRO_0000287437
Regions
	Transmembrane	9 – 29	21	Helical; Potential
	Transmembrane	53 – 73	21	Helical; Potential
	Transmembrane	91 – 111	21	Helical; Potential
	Transmembrane	116 – 136	21	Helical; Potential
	Transmembrane	161 – 181	21	Helical; Potential
	Transmembrane	200 – 220	21	Helical; Potential
Natural variations
	Alternative sequence	1 – 106	106	Missing in isoform 2.		VSP_025461

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 2. Checksum: AC89F1301B0A0048	FASTA	238	26,201
	10 20 30 40 50 60 MLCFLRGMAF VPFLLVTWSS AAFIISYVVA VLSGHVNPFL PYISDTGTTP PESGIFGFMI 70 80 90 100 110 120 NFSAFLGAAT MYTRYKIVEK QNETCYFSTP VFNLVSLALG LVGCIGMGIV ANFQELAVPV 130 140 150 160 170 180 VHDGGALLAF VCGVVYTLLQ SIISYKSCPQ WNSLTTCHVR MAISAVSCAA VVPMIACASL 190 200 210 220 230 ISITKLEWNP KEKDYIYHVV SAICEWTVAF GFIFYFLTFI QDFQSVTLRI STEINDDF
	Isoform 2 [UniParc]. Checksum: FA37881D1038E468	FASTA	132	14,667
	10 20 30 40 50 60 MGIVANFQEL AVPVVHDGGA LLAFVCGVVY TLLQSIISYK SCPQWNSLTT CHVRMAISAV 70 80 90 100 110 120 SCAAVVPMIA CASLISITKL EWNPKEKDYI YHVVSAICEW TVAFGFIFYF LTFIQDFQSV 130 TLRISTEIND DF

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Lung.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK004552 mRNA. Translation: BAB23366.2. BC021433 mRNA. Translation: AAH21433.1.
IPI	IPI00276264. IPI00845672.
RefSeq	NP_082154.2. NM_027878.2.
UniGene	Mm.35450.
3D structure databases
ProteinModelPortal	Q9DC58.
ModBase	Search...
Proteomic databases
PRIDE	Q9DC58.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020249; ENSMUSP00000020249; ENSMUSG00000020057.
GeneID	71712.
KEGG	mmu:71712.
NMPDR	fig|10090.3.peg.14226.
UCSC	uc007grj.1. mouse.
Organism-specific databases
CTD	55332.
MGI	MGI:1918962. Dram1.
Phylogenomic databases
eggNOG	roNOG15566.
GeneTree	ENSGT00530000062905.
InParanoid	Q9DC58.
OMA	FLTFIHD.
PhylomeDB	Q9DC58.
Gene expression databases
ArrayExpress	Q9DC58.
Bgee	Q9DC58.
CleanEx	MM_1200002N14RIK.
Genevestigator	Q9DC58.
Family and domain databases
InterPro	IPR019402. Frag1/DRAM/Sfk1. [Graphical view]
Pfam	PF10277. Frag1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	334303.
SOURCE	Search...

Entry information

Entry name

DRAM1_MOUSE

Accession

Primary (citable) accession number: Q9DC58
Secondary accession number(s): Q78J26

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	July 5, 2004
Last modified:	September 21, 2011
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program