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Protein

Peroxisomal carnitine O-octanoyltransferase

Gene

Crot

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate.1 Publication

Catalytic activityi

Octanoyl-CoA + L-carnitine = CoA + L-octanoylcarnitine.1 Publication

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei327Proton acceptor1 Publication1
Binding sitei406Coenzyme ABy similarity1
Binding sitei439Carnitine1
Binding sitei441Carnitine; via carbonyl oxygen1
Binding sitei452Carnitine1

GO - Molecular functioni

  • carnitine O-octanoyltransferase activity Source: UniProtKB
  • receptor binding Source: MGI

GO - Biological processi

  • carnitine metabolic process Source: UniProtKB
  • coenzyme A metabolic process Source: UniProtKB
  • fatty acid beta-oxidation Source: MGI
  • fatty acid metabolic process Source: UniProtKB
  • fatty acid transport Source: MGI
  • generation of precursor metabolites and energy Source: MGI
  • medium-chain fatty acid metabolic process Source: MGI

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processFatty acid metabolism, Lipid metabolism, Transport

Enzyme and pathway databases

BRENDAi2.3.1.137. 3474.
ReactomeiR-MMU-389887. Beta-oxidation of pristanoyl-CoA.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal carnitine O-octanoyltransferase (EC:2.3.1.137)
Short name:
COT
Gene namesi
Name:Crot
Synonyms:Cot
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1921364. Crot.

Subcellular locationi

GO - Cellular componenti

  • intracellular membrane-bounded organelle Source: MGI
  • mitochondrion Source: MGI
  • peroxisome Source: MGI

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi323C → M: Increases activity with octanoyl-CoA. 1 Publication1
Mutagenesisi335M → A: Slightly decreases activity with octanoyl-CoA. 1 Publication1
Mutagenesisi335M → A: Strongly decreases activity with octanoyl-CoA. 1 Publication1
Mutagenesisi553G → M: Loss of activity with octanoyl-CoA and myristoyl-CoA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002101701 – 612Peroxisomal carnitine O-octanoyltransferaseAdd BLAST612

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei40N6-succinyllysineCombined sources1
Modified residuei57N6-succinyllysineCombined sources1
Modified residuei406N6-acetyllysine; alternateCombined sources1
Modified residuei406N6-succinyllysine; alternateCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9DC50.
MaxQBiQ9DC50.
PaxDbiQ9DC50.
PeptideAtlasiQ9DC50.
PRIDEiQ9DC50.

PTM databases

iPTMnetiQ9DC50.
PhosphoSitePlusiQ9DC50.
SwissPalmiQ9DC50.

Expressioni

Gene expression databases

BgeeiENSMUSG00000003623.
CleanExiMM_CROT.
GenevisibleiQ9DC50. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9DC50. 3 interactors.
MINTiMINT-1850094.
STRINGi10090.ENSMUSP00000003720.

Structurei

Secondary structure

1612
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni13 – 16Combined sources4
Helixi17 – 19Combined sources3
Helixi28 – 39Combined sources12
Helixi40 – 42Combined sources3
Helixi45 – 60Combined sources16
Helixi62 – 76Combined sources15
Beta strandi78 – 81Combined sources4
Helixi82 – 89Combined sources8
Helixi97 – 100Combined sources4
Beta strandi103 – 106Combined sources4
Helixi108 – 111Combined sources4
Helixi121 – 140Combined sources20
Helixi157 – 161Combined sources5
Beta strandi162 – 168Combined sources7
Beta strandi175 – 178Combined sources4
Turni183 – 185Combined sources3
Beta strandi191 – 196Combined sources6
Beta strandi199 – 207Combined sources9
Helixi214 – 229Combined sources16
Helixi237 – 242Combined sources6
Helixi245 – 258Combined sources14
Helixi260 – 271Combined sources12
Beta strandi275 – 278Combined sources4
Helixi291 – 298Combined sources8
Helixi302 – 304Combined sources3
Beta strandi312 – 315Combined sources4
Beta strandi321 – 325Combined sources5
Beta strandi327 – 329Combined sources3
Helixi333 – 348Combined sources16
Turni349 – 351Combined sources3
Beta strandi366 – 368Combined sources3
Helixi374 – 392Combined sources19
Beta strandi394 – 401Combined sources8
Helixi406 – 409Combined sources4
Helixi410 – 412Combined sources3
Helixi416 – 432Combined sources17
Beta strandi438 – 443Combined sources6
Beta strandi452 – 456Combined sources5
Helixi460 – 470Combined sources11
Helixi476 – 498Combined sources23
Turni499 – 501Combined sources3
Helixi504 – 516Combined sources13
Helixi523 – 525Combined sources3
Helixi528 – 532Combined sources5
Turni533 – 537Combined sources5
Beta strandi540 – 545Combined sources6
Beta strandi548 – 550Combined sources3
Beta strandi563 – 570Combined sources8
Beta strandi573 – 581Combined sources9
Helixi589 – 611Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XL7X-ray2.00A/B1-612[»]
1XL8X-ray2.20A/B1-612[»]
1XMCX-ray2.00A/B1-612[»]
1XMDX-ray2.10A/B1-612[»]
ProteinModelPortaliQ9DC50.
SMRiQ9DC50.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9DC50.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni410 – 417Coenzyme A bindingBy similarity8

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi610 – 612Microbody targeting signalSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi534 – 537Poly-Gly4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3718. Eukaryota.
ENOG410ZE4S. LUCA.
GeneTreeiENSGT00760000119220.
HOGENOMiHOG000233845.
HOVERGENiHBG104403.
InParanoidiQ9DC50.
KOiK05940.
OMAiDSIMNYF.
OrthoDBiEOG091G06ND.
PhylomeDBiQ9DC50.
TreeFamiTF313836.

Family and domain databases

InterProiView protein in InterPro
IPR000542. Carn_acyl_trans.
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiView protein in Pfam
PF00755. Carn_acyltransf. 1 hit.
PROSITEiView protein in PROSITE
PS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9DC50-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENQLTKSVE ERTFQYQDSL PSLPVPALEE SLKKYLESVK PFANEDEYKK
60 70 80 90 100
TEEIVQKFQE GAGKRLHQKL LERARGKRNW LEEWWLNVAY LDVRIPSQLN
110 120 130 140 150
VNFVGPCPHF EHYWPAREGT QLERGSMMLW HNLNYWQLLR REKLPVHKSG
160 170 180 190 200
NTPLDMNQFR MLFSTCKVPG ITRDSIMNYF KTESEGHCPT HIAVLCRGRA
210 220 230 240 250
FVFDVLHEGC LITPPELLRQ LTYIHKKCSN EPVGPSIAAL TSEERTRWAK
260 270 280 290 300
AREYLISLDP ENLTLLEKIQ TSLFVYSIED SSPHATPEEY SQVFEMLLGG
310 320 330 340 350
DPSVRWGDKS YNLISFANGI FGCCCDHAPY DAMVMVNIAH YVDERVLETE
360 370 380 390 400
GRWKGSEKVR DIPLPEELVF TVDEKILNDV SQAKAQHLKA ASDLQIAAST
410 420 430 440 450
FTSFGKKLTK EEALHPDTFI QLALQLAYYR LHGRPGCCYE TAMTRYFYHG
460 470 480 490 500
RTETVRSCTV EAVRWCQSMQ DPSASLLERQ QKMLEAFAKH NKMMKDCSHG
510 520 530 540 550
KGFDRHLLGL LLIAKEEGLP VPELFEDPLF SRSGGGGNFV LSTSLVGYLR
560 570 580 590 600
VQGVVVPMVH NGYGFFYHIR DDRFVVACSS WRSCPETDAE KLVQMIFHAF
610
HDMIQLMNTA HL
Length:612
Mass (Da):70,264
Last modified:June 1, 2001 - v1
Checksum:i1C045A419A75C8B6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti459T → A in AAH12308 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004567 mRNA. Translation: BAB23378.1.
BC006593 mRNA. Translation: AAH06593.1.
BC012308 mRNA. Translation: AAH12308.1.
CCDSiCCDS19087.1.
RefSeqiNP_076222.1. NM_023733.3.
UniGeneiMm.28197.

Genome annotation databases

EnsembliENSMUST00000003720; ENSMUSP00000003720; ENSMUSG00000003623.
GeneIDi74114.
KEGGimmu:74114.
UCSCiuc008wkt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004567 mRNA. Translation: BAB23378.1.
BC006593 mRNA. Translation: AAH06593.1.
BC012308 mRNA. Translation: AAH12308.1.
CCDSiCCDS19087.1.
RefSeqiNP_076222.1. NM_023733.3.
UniGeneiMm.28197.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XL7X-ray2.00A/B1-612[»]
1XL8X-ray2.20A/B1-612[»]
1XMCX-ray2.00A/B1-612[»]
1XMDX-ray2.10A/B1-612[»]
ProteinModelPortaliQ9DC50.
SMRiQ9DC50.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9DC50. 3 interactors.
MINTiMINT-1850094.
STRINGi10090.ENSMUSP00000003720.

PTM databases

iPTMnetiQ9DC50.
PhosphoSitePlusiQ9DC50.
SwissPalmiQ9DC50.

Proteomic databases

EPDiQ9DC50.
MaxQBiQ9DC50.
PaxDbiQ9DC50.
PeptideAtlasiQ9DC50.
PRIDEiQ9DC50.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003720; ENSMUSP00000003720; ENSMUSG00000003623.
GeneIDi74114.
KEGGimmu:74114.
UCSCiuc008wkt.1. mouse.

Organism-specific databases

CTDi54677.
MGIiMGI:1921364. Crot.

Phylogenomic databases

eggNOGiKOG3718. Eukaryota.
ENOG410ZE4S. LUCA.
GeneTreeiENSGT00760000119220.
HOGENOMiHOG000233845.
HOVERGENiHBG104403.
InParanoidiQ9DC50.
KOiK05940.
OMAiDSIMNYF.
OrthoDBiEOG091G06ND.
PhylomeDBiQ9DC50.
TreeFamiTF313836.

Enzyme and pathway databases

UniPathwayiUPA00659.
BRENDAi2.3.1.137. 3474.
ReactomeiR-MMU-389887. Beta-oxidation of pristanoyl-CoA.

Miscellaneous databases

ChiTaRSiCrot. mouse.
EvolutionaryTraceiQ9DC50.
PROiPR:Q9DC50.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003623.
CleanExiMM_CROT.
GenevisibleiQ9DC50. MM.

Family and domain databases

InterProiView protein in InterPro
IPR000542. Carn_acyl_trans.
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiView protein in Pfam
PF00755. Carn_acyltransf. 1 hit.
PROSITEiView protein in PROSITE
PS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiOCTC_MOUSE
AccessioniPrimary (citable) accession number: Q9DC50
Secondary accession number(s): Q921I4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 1, 2001
Last modified: May 10, 2017
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.