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Protein

LIM zinc-binding domain-containing Nebulette

Gene

Nebl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Binds to actin and plays an important role in the assembly of the Z-disk. Isoform 2 might play a role in the assembly of focal adhesion (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

Actin-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
LIM zinc-binding domain-containing Nebulette
Alternative name(s):
Actin-binding Z-disk protein
Gene namesi
Name:Nebl
Synonyms:Lnebl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1921353. Nebl.

Subcellular locationi

Isoform 2 :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004175851 – 270LIM zinc-binding domain-containing NebuletteAdd BLAST270

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei96Omega-N-methylarginineCombined sources1
Modified residuei132Omega-N-methylarginineCombined sources1
Modified residuei135PhosphothreonineCombined sources1
Modified residuei230PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

MaxQBiQ9DC07.
PaxDbiQ9DC07.
PeptideAtlasiQ9DC07.
PRIDEiQ9DC07.

PTM databases

iPTMnetiQ9DC07.

Expressioni

Gene expression databases

BgeeiENSMUSG00000053702.
ExpressionAtlasiQ9DC07. baseline and differential.
GenevisibleiQ9DC07. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9DC07. 1 interactor.
MINTiMINT-4128419.
STRINGi10090.ENSMUSP00000028080.

Structurei

3D structure databases

ProteinModelPortaliQ9DC07.
SMRiQ9DC07.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 63LIM zinc-bindingPROSITE-ProRule annotationAdd BLAST61
Repeati61 – 95Nebulin 1Add BLAST35
Repeati97 – 131Nebulin 2Add BLAST35
Repeati133 – 159Nebulin 3Add BLAST27
Domaini210 – 270SH3PROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Contains 1 LIM zinc-binding domain.PROSITE-ProRule annotation
Contains 3 nebulin repeats.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain, Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG1702. Eukaryota.
ENOG4111GQ8. LUCA.
GeneTreeiENSGT00530000062924.
HOGENOMiHOG000006616.
HOVERGENiHBG054636.
OMAiRKAEMEQ.
PhylomeDBiQ9DC07.

Family and domain databases

Gene3Di2.10.110.10. 1 hit.
InterProiIPR000900. Nebulin_repeat.
IPR001452. SH3_domain.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 1 hit.
PF00880. Nebulin. 2 hits.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00132. LIM. 1 hit.
SM00227. NEBU. 3 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
PS51216. NEBULIN. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms exist.
Isoform 2 (identifier: Q9DC07-1) [UniParc]FASTAAdd to basket
Also known as: LIM-Nebulette, LIM-NBEL, LNBEL

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNPQCARCGK VVYPTEKVNC LDKYWHKGCF HCEVCKMALN MNNYKGYEKK
60 70 80 90 100
PYCNAHYPKQ SFTTVADTPE NLRLKQQSEL QSQVKYKRDF EESKGRGFSI
110 120 130 140 150
VTDTPELQRL KRTQEQISNV KYHEDFEKTK GRGFTPVVDD PVTERVRKST
160 170 180 190 200
QVVSDAAYKG VQPHVVEMDR RPGIIVAPVL PGAYQQSHSQ GYGYMHQTSV
210 220 230 240 250
SSMRSMQPPA HLRTYRAMYD YSAQDEDEVS FRDGDYIVNV QPIDDGWMYG
260 270
TVQRTGRTGM LPANYIEFVN
Length:270
Mass (Da):31,113
Last modified:June 1, 2001 - v1
Checksum:i1CC9E10C04E49E4D
GO
Isoform 1 (identifier: Q0II04-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q0II04.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:452
Mass (Da):52,201
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004645 mRNA. Translation: BAB23436.1.
AL935297 Genomic DNA. No translation available.
AL772218, AL845498, AL954131 Genomic DNA. Translation: CAM14501.1.
AL845498, AL772218, AL954131 Genomic DNA. Translation: CAM17882.1.
AL954131, AL772218, AL845498 Genomic DNA. Translation: CAM21080.1.
CH466542 Genomic DNA. Translation: EDL08088.1.
BC025863 mRNA. Translation: AAH25863.1.
CCDSiCCDS15706.1. [Q9DC07-1]
RefSeqiNP_083033.1. NM_028757.2. [Q9DC07-1]
UniGeneiMm.120298.
Mm.256298.
Mm.404039.
Mm.440498.

Genome annotation databases

EnsembliENSMUST00000028080; ENSMUSP00000028080; ENSMUSG00000053702. [Q9DC07-1]
GeneIDi74103.
KEGGimmu:74103.
UCSCiuc008ila.1. mouse. [Q9DC07-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004645 mRNA. Translation: BAB23436.1.
AL935297 Genomic DNA. No translation available.
AL772218, AL845498, AL954131 Genomic DNA. Translation: CAM14501.1.
AL845498, AL772218, AL954131 Genomic DNA. Translation: CAM17882.1.
AL954131, AL772218, AL845498 Genomic DNA. Translation: CAM21080.1.
CH466542 Genomic DNA. Translation: EDL08088.1.
BC025863 mRNA. Translation: AAH25863.1.
CCDSiCCDS15706.1. [Q9DC07-1]
RefSeqiNP_083033.1. NM_028757.2. [Q9DC07-1]
UniGeneiMm.120298.
Mm.256298.
Mm.404039.
Mm.440498.

3D structure databases

ProteinModelPortaliQ9DC07.
SMRiQ9DC07.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9DC07. 1 interactor.
MINTiMINT-4128419.
STRINGi10090.ENSMUSP00000028080.

PTM databases

iPTMnetiQ9DC07.

Proteomic databases

MaxQBiQ9DC07.
PaxDbiQ9DC07.
PeptideAtlasiQ9DC07.
PRIDEiQ9DC07.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028080; ENSMUSP00000028080; ENSMUSG00000053702. [Q9DC07-1]
GeneIDi74103.
KEGGimmu:74103.
UCSCiuc008ila.1. mouse. [Q9DC07-1]

Organism-specific databases

CTDi10529.
MGIiMGI:1921353. Nebl.

Phylogenomic databases

eggNOGiKOG1702. Eukaryota.
ENOG4111GQ8. LUCA.
GeneTreeiENSGT00530000062924.
HOGENOMiHOG000006616.
HOVERGENiHBG054636.
OMAiRKAEMEQ.
PhylomeDBiQ9DC07.

Miscellaneous databases

ChiTaRSiNebl. mouse.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000053702.
ExpressionAtlasiQ9DC07. baseline and differential.
GenevisibleiQ9DC07. MM.

Family and domain databases

Gene3Di2.10.110.10. 1 hit.
InterProiIPR000900. Nebulin_repeat.
IPR001452. SH3_domain.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 1 hit.
PF00880. Nebulin. 2 hits.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00132. LIM. 1 hit.
SM00227. NEBU. 3 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
PS51216. NEBULIN. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLNEBL_MOUSE
AccessioniPrimary (citable) accession number: Q9DC07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.