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Protein

Eukaryotic translation initiation factor 3 subunit K

Gene

Eif3k

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-72649. Translation initiation complex formation.
R-MMU-72689. Formation of a pool of free 40S subunits.
R-MMU-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-MMU-72702. Ribosomal scanning and start codon recognition.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit KUniRule annotation
Short name:
eIF3kUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 12UniRule annotation
eIF-3 p25UniRule annotation
Gene namesi
Name:Eif3k
Synonyms:Eif3s12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1921080. Eif3k.

Subcellular locationi

  • Nucleus UniRule annotation
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation
ChainiPRO_00001235472 – 218Eukaryotic translation initiation factor 3 subunit KAdd BLAST217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineUniRule annotationBy similarity1
Modified residuei28PhosphothreonineBy similarity1
Modified residuei217PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9DBZ5.
PaxDbiQ9DBZ5.
PeptideAtlasiQ9DBZ5.
PRIDEiQ9DBZ5.
TopDownProteomicsiQ9DBZ5-1. [Q9DBZ5-1]

PTM databases

iPTMnetiQ9DBZ5.
PhosphoSitePlusiQ9DBZ5.
SwissPalmiQ9DBZ5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000053565.
ExpressionAtlasiQ9DBZ5. baseline and differential.
GenevisibleiQ9DBZ5. MM.

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon. Interacts with ALKBH4, IFIT1 and IFIT2.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi216289. 3 interactors.
IntActiQ9DBZ5. 2 interactors.
MINTiMINT-4098437.
STRINGi10090.ENSMUSP00000066038.

Structurei

3D structure databases

ProteinModelPortaliQ9DBZ5.
SMRiQ9DBZ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eIF-3 subunit K family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3252. Eukaryota.
ENOG410ZSE4. LUCA.
GeneTreeiENSGT00390000009409.
HOGENOMiHOG000008222.
HOVERGENiHBG052081.
InParanoidiQ9DBZ5.
KOiK15028.
OMAiYVQDQAK.
OrthoDBiEOG091G0IXT.
PhylomeDBiQ9DBZ5.
TreeFamiTF314893.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.25.40.250. 1 hit.
HAMAPiMF_03010. eIF3k. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR033464. CSN8_PSD8_EIF3K.
IPR009374. eIF3k.
IPR016020. Transl_init_fac_sub12_N_euk.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13022. PTHR13022. 1 hit.
PfamiPF10075. CSN8_PSD8_EIF3K. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF48371. SSF48371. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9DBZ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV
60 70 80 90 100
LKLYQFNPAF FQTTVTAQIL LKALTNLPHT DFTLCKCMID QAHQEERPIR
110 120 130 140 150
QILYLGDLLE TCHFQAFWQA LDENMDLLEG ITGFEDSVRK FICHVVGITY
160 170 180 190 200
QHIDRWLLAE MLGDLTDNQL KVWMSKYGWS ADESGQVFIC SQEESIKPKN
210
IVEKIDFDSV SSIMASSQ
Length:218
Mass (Da):25,087
Last modified:June 1, 2001 - v1
Checksum:i84173F6E77F8C294
GO
Isoform 2 (identifier: Q9DBZ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     21-53: Missing.

Note: No experimental confirmation available.
Show »
Length:185
Mass (Da):21,307
Checksum:i509E5FB991EAAEB9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01342521 – 53Missing in isoform 2. 1 PublicationAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004664 mRNA. Translation: BAB23454.1.
BC027638 mRNA. Translation: AAH27638.1.
BC091749 mRNA. Translation: AAH91749.1.
CCDSiCCDS21062.1. [Q9DBZ5-1]
RefSeqiNP_001272871.1. NM_001285942.1. [Q9DBZ5-2]
NP_082935.1. NM_028659.3. [Q9DBZ5-1]
UniGeneiMm.29714.

Genome annotation databases

EnsembliENSMUST00000066070; ENSMUSP00000066038; ENSMUSG00000053565. [Q9DBZ5-1]
ENSMUST00000207683; ENSMUSP00000146940; ENSMUSG00000053565. [Q9DBZ5-2]
GeneIDi73830.
KEGGimmu:73830.
UCSCiuc009gai.2. mouse. [Q9DBZ5-1]
uc009gaj.2. mouse. [Q9DBZ5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004664 mRNA. Translation: BAB23454.1.
BC027638 mRNA. Translation: AAH27638.1.
BC091749 mRNA. Translation: AAH91749.1.
CCDSiCCDS21062.1. [Q9DBZ5-1]
RefSeqiNP_001272871.1. NM_001285942.1. [Q9DBZ5-2]
NP_082935.1. NM_028659.3. [Q9DBZ5-1]
UniGeneiMm.29714.

3D structure databases

ProteinModelPortaliQ9DBZ5.
SMRiQ9DBZ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216289. 3 interactors.
IntActiQ9DBZ5. 2 interactors.
MINTiMINT-4098437.
STRINGi10090.ENSMUSP00000066038.

PTM databases

iPTMnetiQ9DBZ5.
PhosphoSitePlusiQ9DBZ5.
SwissPalmiQ9DBZ5.

Proteomic databases

EPDiQ9DBZ5.
PaxDbiQ9DBZ5.
PeptideAtlasiQ9DBZ5.
PRIDEiQ9DBZ5.
TopDownProteomicsiQ9DBZ5-1. [Q9DBZ5-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000066070; ENSMUSP00000066038; ENSMUSG00000053565. [Q9DBZ5-1]
ENSMUST00000207683; ENSMUSP00000146940; ENSMUSG00000053565. [Q9DBZ5-2]
GeneIDi73830.
KEGGimmu:73830.
UCSCiuc009gai.2. mouse. [Q9DBZ5-1]
uc009gaj.2. mouse. [Q9DBZ5-2]

Organism-specific databases

CTDi27335.
MGIiMGI:1921080. Eif3k.

Phylogenomic databases

eggNOGiKOG3252. Eukaryota.
ENOG410ZSE4. LUCA.
GeneTreeiENSGT00390000009409.
HOGENOMiHOG000008222.
HOVERGENiHBG052081.
InParanoidiQ9DBZ5.
KOiK15028.
OMAiYVQDQAK.
OrthoDBiEOG091G0IXT.
PhylomeDBiQ9DBZ5.
TreeFamiTF314893.

Enzyme and pathway databases

ReactomeiR-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-72649. Translation initiation complex formation.
R-MMU-72689. Formation of a pool of free 40S subunits.
R-MMU-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-MMU-72702. Ribosomal scanning and start codon recognition.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

PROiQ9DBZ5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000053565.
ExpressionAtlasiQ9DBZ5. baseline and differential.
GenevisibleiQ9DBZ5. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.25.40.250. 1 hit.
HAMAPiMF_03010. eIF3k. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR033464. CSN8_PSD8_EIF3K.
IPR009374. eIF3k.
IPR016020. Transl_init_fac_sub12_N_euk.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13022. PTHR13022. 1 hit.
PfamiPF10075. CSN8_PSD8_EIF3K. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3K_MOUSE
AccessioniPrimary (citable) accession number: Q9DBZ5
Secondary accession number(s): Q58EU9, Q8K3A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.